Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U8MYG3|A0A1U8MYG3_GOSHI receptor-like serine/threonine-protein kinase At3g01300 isoform X1 OS=Gossypium hirsutum OX=3635 GN=LOC107942688 PE=3 SV=1
MM1 pKa = 7.6AGAASAQEE9 pKa = 4.02TLRR12 pKa = 11.84IGTEE16 pKa = 3.79GAYY19 pKa = 9.89PPFNYY24 pKa = 9.72TNAAGEE30 pKa = 4.54LIGFDD35 pKa = 3.31IDD37 pKa = 3.63IANALCEE44 pKa = 4.17EE45 pKa = 4.24MQVTCEE51 pKa = 4.12FVRR54 pKa = 11.84SDD56 pKa = 3.09WDD58 pKa = 4.15GIIPGLQNNRR68 pKa = 11.84FDD70 pKa = 5.59AIIASMSITPEE81 pKa = 3.53RR82 pKa = 11.84AEE84 pKa = 3.51QVLFTEE90 pKa = 5.57KK91 pKa = 10.74YY92 pKa = 8.51YY93 pKa = 11.0NSPPAIAVPTDD104 pKa = 3.52SEE106 pKa = 4.29LTEE109 pKa = 4.29ATDD112 pKa = 3.3EE113 pKa = 4.3ALAGLSIGVQTGTTHH128 pKa = 7.64AEE130 pKa = 3.51AAQAYY135 pKa = 8.1FPSADD140 pKa = 3.13VRR142 pKa = 11.84IYY144 pKa = 8.87PTAEE148 pKa = 3.85EE149 pKa = 4.18YY150 pKa = 10.83QLDD153 pKa = 4.05IEE155 pKa = 4.45NGRR158 pKa = 11.84LDD160 pKa = 4.3AVMDD164 pKa = 4.11DD165 pKa = 4.46VIVLGQFIDD174 pKa = 3.88EE175 pKa = 5.01DD176 pKa = 3.79AASCCKK182 pKa = 10.24VLQTLPVDD190 pKa = 3.69PAIFGPGAGIALRR203 pKa = 11.84QGEE206 pKa = 4.28EE207 pKa = 3.68ALAEE211 pKa = 4.1RR212 pKa = 11.84FNAAIQAIRR221 pKa = 11.84EE222 pKa = 4.03NGTYY226 pKa = 10.74AEE228 pKa = 4.18INDD231 pKa = 4.08RR232 pKa = 11.84YY233 pKa = 10.68FDD235 pKa = 3.78FDD237 pKa = 4.08VYY239 pKa = 11.58GDD241 pKa = 3.51

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8HN64|A0A1U8HN64_GOSHI uncharacterized protein LOC107887767 OS=Gossypium hirsutum OX=3635 GN=LOC107887767 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60813

15363

76176

33397352

25

6711

438.4

48.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.569 ± 0.009

1.895 ± 0.004

5.296 ± 0.005

6.538 ± 0.012

4.222 ± 0.006

6.433 ± 0.009

2.368 ± 0.004

5.327 ± 0.006

6.278 ± 0.008

9.761 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.004

4.602 ± 0.006

4.895 ± 0.009

3.794 ± 0.006

5.216 ± 0.007

9.068 ± 0.014

4.85 ± 0.005

6.417 ± 0.006

1.265 ± 0.003

2.769 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski