Colwellia ponticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S3NY46|A0A5S3NY46_9GAMM DNA polymerase I OS=Colwellia ponticola OX=2304625 GN=polA PE=3 SV=1
MM1 pKa = 7.72KK2 pKa = 9.08KK3 pKa = 8.34TLLTVALLSTLSTAAFANQAGDD25 pKa = 3.26ILIRR29 pKa = 11.84GGATMVSPDD38 pKa = 3.73SGQSAILLGGSNATGASMSVDD59 pKa = 4.03DD60 pKa = 4.4NTQLGLNFVYY70 pKa = 10.31FFDD73 pKa = 4.57SNWAIEE79 pKa = 4.34LLAATPFTHH88 pKa = 7.87DD89 pKa = 3.95ATLHH93 pKa = 6.65DD94 pKa = 4.14PTAALGGALGVPGGLDD110 pKa = 3.37GAKK113 pKa = 9.98LANIKK118 pKa = 9.88HH119 pKa = 6.18LPPTLSALYY128 pKa = 10.78YY129 pKa = 10.08FDD131 pKa = 4.11TGTAFKK137 pKa = 9.51PYY139 pKa = 9.82VGAGINYY146 pKa = 7.95TIFFDD151 pKa = 4.66EE152 pKa = 4.65KK153 pKa = 10.91FEE155 pKa = 4.45PAPQTLGLSNLEE167 pKa = 4.03LDD169 pKa = 3.72GSFGYY174 pKa = 9.95SVQVGADD181 pKa = 3.43YY182 pKa = 11.48DD183 pKa = 5.08LGDD186 pKa = 3.5NWSVNVSARR195 pKa = 11.84YY196 pKa = 9.4IDD198 pKa = 3.57ISTDD202 pKa = 3.02VTFDD206 pKa = 3.28VGGDD210 pKa = 3.85SIGSATVDD218 pKa = 3.63VDD220 pKa = 3.44PMVYY224 pKa = 10.27SVMLGYY230 pKa = 8.95TFF232 pKa = 5.14

Molecular weight: 24.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S3PVX4|A0A5S3PVX4_9GAMM M23 family metallopeptidase OS=Colwellia ponticola OX=2304625 GN=FCS21_01825 PE=4 SV=1
MM1 pKa = 7.44LNIFVGRR8 pKa = 11.84ALARR12 pKa = 11.84QILFVGRR19 pKa = 11.84PLARR23 pKa = 11.84QTLFVGRR30 pKa = 11.84ALARR34 pKa = 11.84QLLFVGRR41 pKa = 11.84ALARR45 pKa = 11.84QTLFVGRR52 pKa = 11.84ALARR56 pKa = 11.84QLLFVGRR63 pKa = 11.84ASARR67 pKa = 11.84QVTKK71 pKa = 10.63AVNKK75 pKa = 10.18APQSS79 pKa = 3.55

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3200

0

3200

1042663

12

2628

325.8

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.666 ± 0.047

1.006 ± 0.014

5.452 ± 0.034

5.711 ± 0.034

4.175 ± 0.028

6.295 ± 0.046

2.209 ± 0.018

7.111 ± 0.033

5.958 ± 0.031

10.512 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.022

4.959 ± 0.034

3.565 ± 0.025

4.721 ± 0.039

3.926 ± 0.03

6.809 ± 0.031

5.714 ± 0.024

6.709 ± 0.033

1.073 ± 0.015

3.028 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski