Nitrospina gracilis (strain 3/211)

Taxonomy: cellular organisms; Bacteria; Nitrospinae/Tectomicrobia group; Nitrospinae; Nitrospinia; Nitrospinales; Nitrospinaceae; Nitrospina; Nitrospina gracilis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2962 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1YJS8|M1YJS8_NITG3 Putative Nitrite oxidoreductase gamma subunit (Modular protein) OS=Nitrospina gracilis (strain 3/211) OX=1266370 GN=NITGR_360061 PE=4 SV=1
MM1 pKa = 7.42GFLVSFSLDD10 pKa = 3.27HH11 pKa = 7.38RR12 pKa = 11.84DD13 pKa = 3.54TLILMHH19 pKa = 6.06VAGYY23 pKa = 10.73LDD25 pKa = 4.07DD26 pKa = 5.3LLWVLYY32 pKa = 8.79FSIASPPPFGSNAFADD48 pKa = 4.13GDD50 pKa = 4.07DD51 pKa = 3.52

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1YXS4|M1YXS4_NITG3 30S ribosomal protein S20 OS=Nitrospina gracilis (strain 3/211) OX=1266370 GN=rpsT PE=3 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.73MKK5 pKa = 9.76TNKK8 pKa = 9.68SAAKK12 pKa = 9.87RR13 pKa = 11.84FRR15 pKa = 11.84KK16 pKa = 8.37TGTGKK21 pKa = 9.67IRR23 pKa = 11.84RR24 pKa = 11.84NAAFTSHH31 pKa = 7.03ILTSKK36 pKa = 4.66TTKK39 pKa = 9.98RR40 pKa = 11.84KK41 pKa = 9.29RR42 pKa = 11.84NLRR45 pKa = 11.84RR46 pKa = 11.84SRR48 pKa = 11.84TLTSGDD54 pKa = 3.16AKK56 pKa = 10.45RR57 pKa = 11.84IKK59 pKa = 10.67ALLPNN64 pKa = 4.31

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2962

0

2962

888891

20

1989

300.1

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.855 ± 0.046

1.021 ± 0.016

5.586 ± 0.036

6.995 ± 0.043

4.51 ± 0.035

7.474 ± 0.04

2.376 ± 0.023

5.783 ± 0.039

5.685 ± 0.049

10.077 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.023

3.813 ± 0.032

4.793 ± 0.033

3.725 ± 0.03

5.808 ± 0.036

5.409 ± 0.031

5.22 ± 0.033

7.123 ± 0.034

1.241 ± 0.018

2.922 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski