Amino acid dipepetide frequency for Aureobasidium pullulans EXF-150

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.22AlaAla: 9.22 ± 0.054	1.159AlaCys: 1.159 ± 0.016	4.314AlaAsp: 4.314 ± 0.029	5.139AlaGlu: 5.139 ± 0.038	3.304AlaPhe: 3.304 ± 0.031	5.617AlaGly: 5.617 ± 0.04	1.842AlaHis: 1.842 ± 0.021	4.37AlaIle: 4.37 ± 0.03	4.209AlaLys: 4.209 ± 0.034	7.852AlaLeu: 7.852 ± 0.05	2.076AlaMet: 2.076 ± 0.018	3.164AlaAsn: 3.164 ± 0.026	4.906AlaPro: 4.906 ± 0.045	3.674AlaGln: 3.674 ± 0.032	4.809AlaArg: 4.809 ± 0.034	7.59AlaSer: 7.59 ± 0.05	5.437AlaThr: 5.437 ± 0.036	5.322AlaVal: 5.322 ± 0.033	1.202AlaTrp: 1.202 ± 0.016	2.312AlaTyr: 2.312 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	0.98CysAla: 0.98 ± 0.015	0.27CysCys: 0.27 ± 0.009	0.7CysAsp: 0.7 ± 0.013	0.602CysGlu: 0.602 ± 0.011	0.589CysPhe: 0.589 ± 0.012	0.95CysGly: 0.95 ± 0.016	0.345CysHis: 0.345 ± 0.008	0.744CysIle: 0.744 ± 0.012	0.594CysLys: 0.594 ± 0.012	1.304CysLeu: 1.304 ± 0.014	0.292CysMet: 0.292 ± 0.007	0.478CysAsn: 0.478 ± 0.011	0.669CysPro: 0.669 ± 0.012	0.467CysGln: 0.467 ± 0.009	0.745CysArg: 0.745 ± 0.013	0.955CysSer: 0.955 ± 0.014	0.748CysThr: 0.748 ± 0.014	0.811CysVal: 0.811 ± 0.012	0.205CysTrp: 0.205 ± 0.006	0.364CysTyr: 0.364 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	4.894AspAla: 4.894 ± 0.032	0.658AspCys: 0.658 ± 0.012	4.374AspAsp: 4.374 ± 0.044	4.528AspGlu: 4.528 ± 0.039	2.312AspPhe: 2.312 ± 0.022	3.783AspGly: 3.783 ± 0.031	1.3AspHis: 1.3 ± 0.017	3.052AspIle: 3.052 ± 0.028	2.578AspLys: 2.578 ± 0.023	5.117AspLeu: 5.117 ± 0.037	1.438AspMet: 1.438 ± 0.02	2.025AspAsn: 2.025 ± 0.019	3.134AspPro: 3.134 ± 0.028	2.076AspGln: 2.076 ± 0.017	2.934AspArg: 2.934 ± 0.028	4.1AspSer: 4.1 ± 0.031	3.096AspThr: 3.096 ± 0.023	3.745AspVal: 3.745 ± 0.027	0.929AspTrp: 0.929 ± 0.013	1.665AspTyr: 1.665 ± 0.016	0.0AspXaa: 0.0 ± 0.0
Glu	5.296GluAla: 5.296 ± 0.032	0.596GluCys: 0.596 ± 0.011	4.384GluAsp: 4.384 ± 0.037	5.253GluGlu: 5.253 ± 0.048	1.88GluPhe: 1.88 ± 0.021	3.597GluGly: 3.597 ± 0.026	1.485GluHis: 1.485 ± 0.017	2.932GluIle: 2.932 ± 0.028	3.793GluLys: 3.793 ± 0.034	4.999GluLeu: 4.999 ± 0.046	1.524GluMet: 1.524 ± 0.017	2.211GluAsn: 2.211 ± 0.021	2.555GluPro: 2.555 ± 0.025	2.67GluGln: 2.67 ± 0.025	3.676GluArg: 3.676 ± 0.037	4.058GluSer: 4.058 ± 0.029	3.4GluThr: 3.4 ± 0.029	3.464GluVal: 3.464 ± 0.028	0.863GluTrp: 0.863 ± 0.014	1.662GluTyr: 1.662 ± 0.017	0.0GluXaa: 0.0 ± 0.0
Phe	3.315PheAla: 3.315 ± 0.029	0.591PheCys: 0.591 ± 0.012	2.36PheAsp: 2.36 ± 0.022	2.227PheGlu: 2.227 ± 0.022	1.663PhePhe: 1.663 ± 0.021	2.955PheGly: 2.955 ± 0.03	0.875PheHis: 0.875 ± 0.013	1.782PheIle: 1.782 ± 0.024	1.557PheLys: 1.557 ± 0.017	3.364PheLeu: 3.364 ± 0.026	0.81PheMet: 0.81 ± 0.013	1.487PheAsn: 1.487 ± 0.019	1.835PhePro: 1.835 ± 0.02	1.314PheGln: 1.314 ± 0.016	1.865PheArg: 1.865 ± 0.018	3.066PheSer: 3.066 ± 0.028	2.198PheThr: 2.198 ± 0.019	2.472PheVal: 2.472 ± 0.024	0.668PheTrp: 0.668 ± 0.012	1.111PheTyr: 1.111 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.16GlyAla: 5.16 ± 0.039	0.901GlyCys: 0.901 ± 0.013	3.415GlyAsp: 3.415 ± 0.025	3.362GlyGlu: 3.362 ± 0.026	2.794GlyPhe: 2.794 ± 0.028	5.298GlyGly: 5.298 ± 0.052	1.617GlyHis: 1.617 ± 0.017	3.432GlyIle: 3.432 ± 0.031	3.422GlyLys: 3.422 ± 0.028	5.845GlyLeu: 5.845 ± 0.035	1.636GlyMet: 1.636 ± 0.023	2.524GlyAsn: 2.524 ± 0.027	2.989GlyPro: 2.989 ± 0.027	2.53GlyGln: 2.53 ± 0.026	3.765GlyArg: 3.765 ± 0.036	5.357GlySer: 5.357 ± 0.036	3.793GlyThr: 3.793 ± 0.032	4.25GlyVal: 4.25 ± 0.034	1.14GlyTrp: 1.14 ± 0.016	2.123GlyTyr: 2.123 ± 0.024	0.001GlyXaa: 0.001 ± 0.0
His	1.95HisAla: 1.95 ± 0.02	0.345HisCys: 0.345 ± 0.009	1.449HisAsp: 1.449 ± 0.019	1.433HisGlu: 1.433 ± 0.017	0.93HisPhe: 0.93 ± 0.011	1.658HisGly: 1.658 ± 0.019	0.881HisHis: 0.881 ± 0.015	1.24HisIle: 1.24 ± 0.019	1.025HisLys: 1.025 ± 0.014	2.301HisLeu: 2.301 ± 0.022	0.542HisMet: 0.542 ± 0.012	0.956HisAsn: 0.956 ± 0.014	1.573HisPro: 1.573 ± 0.017	1.054HisGln: 1.054 ± 0.015	1.433HisArg: 1.433 ± 0.017	1.821HisSer: 1.821 ± 0.02	1.378HisThr: 1.378 ± 0.016	1.5HisVal: 1.5 ± 0.017	0.368HisTrp: 0.368 ± 0.009	0.702HisTyr: 0.702 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.393IleAla: 4.393 ± 0.036	0.78IleCys: 0.78 ± 0.012	2.938IleAsp: 2.938 ± 0.027	2.875IleGlu: 2.875 ± 0.025	1.997IlePhe: 1.997 ± 0.025	3.143IleGly: 3.143 ± 0.032	1.212IleHis: 1.212 ± 0.015	2.534IleIle: 2.534 ± 0.026	2.385IleLys: 2.385 ± 0.024	4.371IleLeu: 4.371 ± 0.033	1.088IleMet: 1.088 ± 0.014	1.962IleAsn: 1.962 ± 0.017	2.868IlePro: 2.868 ± 0.024	1.857IleGln: 1.857 ± 0.019	2.653IleArg: 2.653 ± 0.025	3.949IleSer: 3.949 ± 0.027	2.972IleThr: 2.972 ± 0.025	3.149IleVal: 3.149 ± 0.028	0.743IleTrp: 0.743 ± 0.012	1.417IleTyr: 1.417 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.501LysAla: 4.501 ± 0.035	0.504LysCys: 0.504 ± 0.012	3.027LysAsp: 3.027 ± 0.026	3.4LysGlu: 3.4 ± 0.03	1.477LysPhe: 1.477 ± 0.016	2.946LysGly: 2.946 ± 0.028	1.287LysHis: 1.287 ± 0.016	2.359LysIle: 2.359 ± 0.021	3.485LysLys: 3.485 ± 0.04	4.074LysLeu: 4.074 ± 0.03	1.064LysMet: 1.064 ± 0.015	1.853LysAsn: 1.853 ± 0.019	2.776LysPro: 2.776 ± 0.024	2.109LysGln: 2.109 ± 0.022	3.368LysArg: 3.368 ± 0.027	3.66LysSer: 3.66 ± 0.029	3.086LysThr: 3.086 ± 0.029	2.91LysVal: 2.91 ± 0.028	0.673LysTrp: 0.673 ± 0.011	1.342LysTyr: 1.342 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	7.742LeuAla: 7.742 ± 0.045	1.271LeuCys: 1.271 ± 0.015	5.295LeuAsp: 5.295 ± 0.036	5.353LeuGlu: 5.353 ± 0.041	3.316LeuPhe: 3.316 ± 0.03	5.617LeuGly: 5.617 ± 0.04	2.211LeuHis: 2.211 ± 0.022	3.935LeuIle: 3.935 ± 0.03	4.149LeuLys: 4.149 ± 0.029	8.162LeuLeu: 8.162 ± 0.06	1.835LeuMet: 1.835 ± 0.019	3.249LeuAsn: 3.249 ± 0.026	5.439LeuPro: 5.439 ± 0.031	3.835LeuGln: 3.835 ± 0.032	5.51LeuArg: 5.51 ± 0.037	7.291LeuSer: 7.291 ± 0.047	4.872LeuThr: 4.872 ± 0.033	5.26LeuVal: 5.26 ± 0.032	1.177LeuTrp: 1.177 ± 0.014	2.361LeuTyr: 2.361 ± 0.022	0.0LeuXaa: 0.0 ± 0.0
Met	2.253MetAla: 2.253 ± 0.023	0.274MetCys: 0.274 ± 0.007	1.316MetAsp: 1.316 ± 0.016	1.24MetGlu: 1.24 ± 0.015	0.823MetPhe: 0.823 ± 0.014	1.434MetGly: 1.434 ± 0.019	0.554MetHis: 0.554 ± 0.009	1.078MetIle: 1.078 ± 0.014	0.996MetLys: 0.996 ± 0.013	1.978MetLeu: 1.978 ± 0.021	0.622MetMet: 0.622 ± 0.012	0.868MetAsn: 0.868 ± 0.013	1.399MetPro: 1.399 ± 0.017	0.984MetGln: 0.984 ± 0.015	1.277MetArg: 1.277 ± 0.015	2.09MetSer: 2.09 ± 0.02	1.365MetThr: 1.365 ± 0.015	1.332MetVal: 1.332 ± 0.017	0.272MetTrp: 0.272 ± 0.007	0.584MetTyr: 0.584 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.34AsnAla: 3.34 ± 0.028	0.444AsnCys: 0.444 ± 0.01	2.135AsnAsp: 2.135 ± 0.021	1.994AsnGlu: 1.994 ± 0.018	1.454AsnPhe: 1.454 ± 0.018	2.817AsnGly: 2.817 ± 0.029	0.934AsnHis: 0.934 ± 0.014	2.177AsnIle: 2.177 ± 0.022	1.78AsnLys: 1.78 ± 0.017	3.372AsnLeu: 3.372 ± 0.025	0.928AsnMet: 0.928 ± 0.013	1.759AsnAsn: 1.759 ± 0.022	2.376AsnPro: 2.376 ± 0.021	1.423AsnGln: 1.423 ± 0.017	1.885AsnArg: 1.885 ± 0.018	2.861AsnSer: 2.861 ± 0.027	2.485AsnThr: 2.485 ± 0.022	2.402AsnVal: 2.402 ± 0.021	0.548AsnTrp: 0.548 ± 0.01	1.107AsnTyr: 1.107 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.189ProAla: 5.189 ± 0.04	0.533ProCys: 0.533 ± 0.012	3.05ProAsp: 3.05 ± 0.028	3.696ProGlu: 3.696 ± 0.03	2.026ProPhe: 2.026 ± 0.02	3.446ProGly: 3.446 ± 0.029	1.307ProHis: 1.307 ± 0.017	2.473ProIle: 2.473 ± 0.023	2.673ProLys: 2.673 ± 0.031	4.555ProLeu: 4.555 ± 0.033	1.118ProMet: 1.118 ± 0.016	2.127ProAsn: 2.127 ± 0.023	4.59ProPro: 4.59 ± 0.068	2.555ProGln: 2.555 ± 0.029	3.161ProArg: 3.161 ± 0.027	5.979ProSer: 5.979 ± 0.041	4.042ProThr: 4.042 ± 0.035	3.471ProVal: 3.471 ± 0.027	0.712ProTrp: 0.712 ± 0.013	1.503ProTyr: 1.503 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.674GlnAla: 3.674 ± 0.033	0.46GlnCys: 0.46 ± 0.01	2.314GlnAsp: 2.314 ± 0.025	2.491GlnGlu: 2.491 ± 0.025	1.252GlnPhe: 1.252 ± 0.016	2.454GlnGly: 2.454 ± 0.022	1.23GlnHis: 1.23 ± 0.017	1.947GlnIle: 1.947 ± 0.021	2.142GlnLys: 2.142 ± 0.025	3.42GlnLeu: 3.42 ± 0.029	0.952GlnMet: 0.952 ± 0.015	1.665GlnAsn: 1.665 ± 0.019	2.596GlnPro: 2.596 ± 0.028	2.814GlnGln: 2.814 ± 0.059	2.642GlnArg: 2.642 ± 0.029	3.312GlnSer: 3.312 ± 0.027	2.492GlnThr: 2.492 ± 0.022	2.172GlnVal: 2.172 ± 0.021	0.572GlnTrp: 0.572 ± 0.012	1.201GlnTyr: 1.201 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	4.492ArgAla: 4.492 ± 0.033	0.737ArgCys: 0.737 ± 0.014	3.155ArgAsp: 3.155 ± 0.03	3.507ArgGlu: 3.507 ± 0.032	2.072ArgPhe: 2.072 ± 0.021	3.364ArgGly: 3.364 ± 0.03	1.55ArgHis: 1.55 ± 0.017	2.795ArgIle: 2.795 ± 0.024	3.468ArgLys: 3.468 ± 0.031	5.259ArgLeu: 5.259 ± 0.036	1.353ArgMet: 1.353 ± 0.014	2.259ArgAsn: 2.259 ± 0.022	3.374ArgPro: 3.374 ± 0.03	2.652ArgGln: 2.652 ± 0.025	4.604ArgArg: 4.604 ± 0.045	4.627ArgSer: 4.627 ± 0.037	3.235ArgThr: 3.235 ± 0.029	3.17ArgVal: 3.17 ± 0.028	0.876ArgTrp: 0.876 ± 0.012	1.593ArgTyr: 1.593 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	7.101SerAla: 7.101 ± 0.051	0.968SerCys: 0.968 ± 0.015	4.373SerAsp: 4.373 ± 0.033	4.078SerGlu: 4.078 ± 0.03	3.085SerPhe: 3.085 ± 0.026	5.389SerGly: 5.389 ± 0.038	1.983SerHis: 1.983 ± 0.02	4.118SerIle: 4.118 ± 0.031	3.837SerLys: 3.837 ± 0.031	7.047SerLeu: 7.047 ± 0.045	1.802SerMet: 1.802 ± 0.021	3.192SerAsn: 3.192 ± 0.029	5.324SerPro: 5.324 ± 0.047	3.291SerGln: 3.291 ± 0.027	4.868SerArg: 4.868 ± 0.038	9.197SerSer: 9.197 ± 0.096	6.0SerThr: 6.0 ± 0.049	4.661SerVal: 4.661 ± 0.039	1.137SerTrp: 1.137 ± 0.017	2.141SerTyr: 2.141 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	5.431ThrAla: 5.431 ± 0.033	0.826ThrCys: 0.826 ± 0.013	2.901ThrAsp: 2.901 ± 0.026	3.048ThrGlu: 3.048 ± 0.026	2.341ThrPhe: 2.341 ± 0.022	4.072ThrGly: 4.072 ± 0.032	1.385ThrHis: 1.385 ± 0.016	3.243ThrIle: 3.243 ± 0.025	2.8ThrLys: 2.8 ± 0.029	5.372ThrLeu: 5.372 ± 0.036	1.225ThrMet: 1.225 ± 0.016	2.322ThrAsn: 2.322 ± 0.024	4.325ThrPro: 4.325 ± 0.034	2.298ThrGln: 2.298 ± 0.024	3.15ThrArg: 3.15 ± 0.024	5.776ThrSer: 5.776 ± 0.05	4.62ThrThr: 4.62 ± 0.038	3.728ThrVal: 3.728 ± 0.03	0.874ThrTrp: 0.874 ± 0.012	1.689ThrTyr: 1.689 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	5.221ValAla: 5.221 ± 0.039	0.847ValCys: 0.847 ± 0.012	3.669ValAsp: 3.669 ± 0.03	3.794ValGlu: 3.794 ± 0.029	2.447ValPhe: 2.447 ± 0.024	3.826ValGly: 3.826 ± 0.031	1.395ValHis: 1.395 ± 0.018	2.888ValIle: 2.888 ± 0.029	3.043ValLys: 3.043 ± 0.027	5.476ValLeu: 5.476 ± 0.038	1.385ValMet: 1.385 ± 0.016	2.256ValAsn: 2.256 ± 0.022	3.393ValPro: 3.393 ± 0.022	2.435ValGln: 2.435 ± 0.021	3.331ValArg: 3.331 ± 0.026	4.693ValSer: 4.693 ± 0.034	3.498ValThr: 3.498 ± 0.035	4.206ValVal: 4.206 ± 0.037	0.873ValTrp: 0.873 ± 0.014	1.758ValTyr: 1.758 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	1.1TrpAla: 1.1 ± 0.014	0.216TrpCys: 0.216 ± 0.007	0.851TrpAsp: 0.851 ± 0.013	0.783TrpGlu: 0.783 ± 0.014	0.562TrpPhe: 0.562 ± 0.011	0.881TrpGly: 0.881 ± 0.016	0.372TrpHis: 0.372 ± 0.009	0.793TrpIle: 0.793 ± 0.013	0.805TrpLys: 0.805 ± 0.012	1.374TrpLeu: 1.374 ± 0.017	0.391TrpMet: 0.391 ± 0.01	0.654TrpAsn: 0.654 ± 0.01	0.59TrpPro: 0.59 ± 0.01	0.622TrpGln: 0.622 ± 0.011	0.912TrpArg: 0.912 ± 0.014	1.147TrpSer: 1.147 ± 0.017	0.967TrpThr: 0.967 ± 0.014	0.808TrpVal: 0.808 ± 0.013	0.276TrpTrp: 0.276 ± 0.008	0.444TrpTyr: 0.444 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.267TyrAla: 2.267 ± 0.022	0.43TyrCys: 0.43 ± 0.009	1.715TyrAsp: 1.715 ± 0.018	1.495TyrGlu: 1.495 ± 0.019	1.191TyrPhe: 1.191 ± 0.016	2.104TyrGly: 2.104 ± 0.025	0.772TyrHis: 0.772 ± 0.013	1.397TyrIle: 1.397 ± 0.018	1.162TyrLys: 1.162 ± 0.014	2.638TyrLeu: 2.638 ± 0.025	0.656TyrMet: 0.656 ± 0.011	1.211TyrAsn: 1.211 ± 0.017	1.498TyrPro: 1.498 ± 0.02	1.143TyrGln: 1.143 ± 0.016	1.552TyrArg: 1.552 ± 0.018	2.066TyrSer: 2.066 ± 0.022	1.741TyrThr: 1.741 ± 0.02	1.602TyrVal: 1.602 ± 0.018	0.441TyrTrp: 0.441 ± 0.011	0.961TyrTyr: 0.961 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11844 proteins (5256702 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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