Corynebacterium phage phi16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8D5N6|A0A1P8D5N6_9CAUD Uncharacterized protein OS=Corynebacterium phage phi16 OX=1927022 GN=phi16_gp014 PE=4 SV=1
MM1 pKa = 6.3TTQAIATIIWNNDD14 pKa = 2.49SDD16 pKa = 4.36NLIEE20 pKa = 4.15SHH22 pKa = 6.67EE23 pKa = 4.5MIRR26 pKa = 11.84DD27 pKa = 3.62DD28 pKa = 5.37NGNDD32 pKa = 3.52TIAKK36 pKa = 8.79IVTSTGHH43 pKa = 5.64IAAVGTEE50 pKa = 3.75EE51 pKa = 4.35DD52 pKa = 4.15AEE54 pKa = 4.52GEE56 pKa = 4.25YY57 pKa = 9.69LTYY60 pKa = 10.56SIYY63 pKa = 11.09APGDD67 pKa = 3.3EE68 pKa = 4.62TMTDD72 pKa = 3.75GPITTDD78 pKa = 2.86GFEE81 pKa = 4.06ITSEE85 pKa = 3.77EE86 pKa = 4.18DD87 pKa = 3.43AADD90 pKa = 3.59HH91 pKa = 6.24LAEE94 pKa = 4.67ILSKK98 pKa = 10.83FF99 pKa = 3.56

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8D5T6|A0A1P8D5T6_9CAUD Uncharacterized protein OS=Corynebacterium phage phi16 OX=1927022 GN=phi16_gp090 PE=4 SV=1
MM1 pKa = 7.29GLVEE5 pKa = 4.9VVATRR10 pKa = 11.84NILFGQKK17 pKa = 8.09ITVRR21 pKa = 11.84ANYY24 pKa = 7.98YY25 pKa = 7.66VCSRR29 pKa = 11.84FGSFLGNRR37 pKa = 11.84RR38 pKa = 11.84FFLGKK43 pKa = 10.55GGVLTLKK50 pKa = 10.04MKK52 pKa = 9.83TKK54 pKa = 10.07HH55 pKa = 5.83LQVGDD60 pKa = 4.81RR61 pKa = 11.84ITMDD65 pKa = 3.47QLVMGARR72 pKa = 11.84DD73 pKa = 3.36WTKK76 pKa = 10.5IPWEE80 pKa = 3.97WAA82 pKa = 3.19

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

17841

43

2151

176.6

19.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.669 ± 0.583

0.465 ± 0.08

6.737 ± 0.282

7.808 ± 0.276

3.352 ± 0.153

7.062 ± 0.353

2.259 ± 0.212

5.796 ± 0.177

5.358 ± 0.198

8.066 ± 0.177

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.105

3.447 ± 0.142

4.63 ± 0.312

3.643 ± 0.181

6.238 ± 0.331

5.885 ± 0.163

5.936 ± 0.241

6.754 ± 0.194

2.007 ± 0.116

2.18 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski