Amino acid dipepetide frequency for Corynebacterium phage phi16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.715AlaAla: 11.715 ± 1.352
0.336AlaCys: 0.336 ± 0.133
5.437AlaAsp: 5.437 ± 0.627
9.081AlaGlu: 9.081 ± 0.865
2.803AlaPhe: 2.803 ± 0.464
6.783AlaGly: 6.783 ± 0.704
2.13AlaHis: 2.13 ± 0.343
4.989AlaIle: 4.989 ± 0.727
4.877AlaLys: 4.877 ± 0.635
8.128AlaLeu: 8.128 ± 0.694
2.354AlaMet: 2.354 ± 0.347
2.859AlaAsn: 2.859 ± 0.386
3.756AlaPro: 3.756 ± 0.464
4.204AlaGln: 4.204 ± 0.805
5.998AlaArg: 5.998 ± 0.789
5.605AlaSer: 5.605 ± 0.606
5.774AlaThr: 5.774 ± 0.645
5.717AlaVal: 5.717 ± 0.475
2.186AlaTrp: 2.186 ± 0.37
1.962AlaTyr: 1.962 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.168CysAla: 0.168 ± 0.099
0.0CysCys: 0.0 ± 0.0
0.392CysAsp: 0.392 ± 0.139
0.28CysGlu: 0.28 ± 0.182
0.168CysPhe: 0.168 ± 0.088
0.561CysGly: 0.561 ± 0.192
0.168CysHis: 0.168 ± 0.139
0.28CysIle: 0.28 ± 0.134
0.168CysLys: 0.168 ± 0.098
0.224CysLeu: 0.224 ± 0.102
0.168CysMet: 0.168 ± 0.101
0.112CysAsn: 0.112 ± 0.072
0.168CysPro: 0.168 ± 0.095
0.168CysGln: 0.168 ± 0.095
0.392CysArg: 0.392 ± 0.148
0.448CysSer: 0.448 ± 0.159
0.392CysThr: 0.392 ± 0.181
0.224CysVal: 0.224 ± 0.105
0.112CysTrp: 0.112 ± 0.074
0.056CysTyr: 0.056 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
6.166AspAla: 6.166 ± 0.655
0.224AspCys: 0.224 ± 0.129
4.821AspAsp: 4.821 ± 0.739
5.213AspGlu: 5.213 ± 0.574
2.466AspPhe: 2.466 ± 0.367
5.774AspGly: 5.774 ± 0.423
1.794AspHis: 1.794 ± 0.324
3.531AspIle: 3.531 ± 0.314
2.466AspLys: 2.466 ± 0.352
5.774AspLeu: 5.774 ± 0.566
1.345AspMet: 1.345 ± 0.198
2.018AspAsn: 2.018 ± 0.294
3.868AspPro: 3.868 ± 0.505
2.859AspGln: 2.859 ± 0.352
4.821AspArg: 4.821 ± 0.586
4.092AspSer: 4.092 ± 0.416
2.747AspThr: 2.747 ± 0.415
4.428AspVal: 4.428 ± 0.589
1.065AspTrp: 1.065 ± 0.24
1.906AspTyr: 1.906 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
6.726GluAla: 6.726 ± 0.714
0.448GluCys: 0.448 ± 0.147
4.989GluAsp: 4.989 ± 0.477
7.399GluGlu: 7.399 ± 0.879
2.466GluPhe: 2.466 ± 0.437
4.596GluGly: 4.596 ± 0.509
1.738GluHis: 1.738 ± 0.323
4.821GluIle: 4.821 ± 0.475
5.381GluLys: 5.381 ± 0.688
6.11GluLeu: 6.11 ± 0.593
2.747GluMet: 2.747 ± 0.346
3.307GluAsn: 3.307 ± 0.427
2.186GluPro: 2.186 ± 0.365
2.915GluGln: 2.915 ± 0.328
3.924GluArg: 3.924 ± 0.518
4.709GluSer: 4.709 ± 0.62
4.316GluThr: 4.316 ± 0.442
5.549GluVal: 5.549 ± 0.509
1.626GluTrp: 1.626 ± 0.283
2.13GluTyr: 2.13 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
3.195PheAla: 3.195 ± 0.39
0.168PheCys: 0.168 ± 0.091
2.635PheAsp: 2.635 ± 0.39
1.794PheGlu: 1.794 ± 0.258
0.953PhePhe: 0.953 ± 0.223
2.298PheGly: 2.298 ± 0.545
1.177PheHis: 1.177 ± 0.315
1.738PheIle: 1.738 ± 0.299
1.738PheLys: 1.738 ± 0.351
2.242PheLeu: 2.242 ± 0.377
1.177PheMet: 1.177 ± 0.313
1.065PheAsn: 1.065 ± 0.198
1.57PhePro: 1.57 ± 0.242
0.897PheGln: 0.897 ± 0.192
1.962PheArg: 1.962 ± 0.317
2.41PheSer: 2.41 ± 0.443
3.083PheThr: 3.083 ± 0.388
2.186PheVal: 2.186 ± 0.409
0.448PheTrp: 0.448 ± 0.166
0.785PheTyr: 0.785 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
5.493GlyAla: 5.493 ± 0.898
0.448GlyCys: 0.448 ± 0.195
4.484GlyAsp: 4.484 ± 0.471
3.98GlyGlu: 3.98 ± 0.475
2.915GlyPhe: 2.915 ± 0.393
5.157GlyGly: 5.157 ± 0.795
1.457GlyHis: 1.457 ± 0.304
4.036GlyIle: 4.036 ± 0.877
3.475GlyLys: 3.475 ± 0.35
6.614GlyLeu: 6.614 ± 0.666
2.074GlyMet: 2.074 ± 0.293
2.41GlyAsn: 2.41 ± 0.384
2.915GlyPro: 2.915 ± 0.429
2.186GlyGln: 2.186 ± 0.39
5.213GlyArg: 5.213 ± 0.692
4.148GlySer: 4.148 ± 0.415
4.148GlyThr: 4.148 ± 0.56
6.166GlyVal: 6.166 ± 0.503
1.794GlyTrp: 1.794 ± 0.481
1.513GlyTyr: 1.513 ± 0.283
0.0GlyXaa: 0.0 ± 0.0
His
1.289HisAla: 1.289 ± 0.261
0.0HisCys: 0.0 ± 0.0
1.233HisAsp: 1.233 ± 0.28
1.513HisGlu: 1.513 ± 0.303
0.785HisPhe: 0.785 ± 0.184
1.345HisGly: 1.345 ± 0.348
0.897HisHis: 0.897 ± 0.261
1.289HisIle: 1.289 ± 0.317
0.561HisLys: 0.561 ± 0.152
2.13HisLeu: 2.13 ± 0.445
0.561HisMet: 0.561 ± 0.164
0.729HisAsn: 0.729 ± 0.208
1.794HisPro: 1.794 ± 0.323
0.897HisGln: 0.897 ± 0.243
1.682HisArg: 1.682 ± 0.419
1.233HisSer: 1.233 ± 0.258
1.289HisThr: 1.289 ± 0.291
1.85HisVal: 1.85 ± 0.332
0.729HisTrp: 0.729 ± 0.211
0.785HisTyr: 0.785 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
5.774IleAla: 5.774 ± 0.669
0.504IleCys: 0.504 ± 0.158
4.428IleAsp: 4.428 ± 0.475
4.989IleGlu: 4.989 ± 0.537
2.074IlePhe: 2.074 ± 0.301
4.372IleGly: 4.372 ± 0.554
0.841IleHis: 0.841 ± 0.189
2.747IleIle: 2.747 ± 0.361
2.578IleLys: 2.578 ± 0.404
4.26IleLeu: 4.26 ± 0.395
1.289IleMet: 1.289 ± 0.286
2.41IleAsn: 2.41 ± 0.385
3.027IlePro: 3.027 ± 0.478
1.794IleGln: 1.794 ± 0.408
3.419IleArg: 3.419 ± 0.347
3.98IleSer: 3.98 ± 0.533
4.26IleThr: 4.26 ± 0.534
3.531IleVal: 3.531 ± 0.399
0.504IleTrp: 0.504 ± 0.126
1.177IleTyr: 1.177 ± 0.224
0.0IleXaa: 0.0 ± 0.0
Lys
5.661LysAla: 5.661 ± 0.586
0.112LysCys: 0.112 ± 0.086
3.083LysAsp: 3.083 ± 0.49
4.316LysGlu: 4.316 ± 0.51
2.186LysPhe: 2.186 ± 0.37
2.41LysGly: 2.41 ± 0.398
1.233LysHis: 1.233 ± 0.272
3.419LysIle: 3.419 ± 0.414
3.7LysLys: 3.7 ± 0.434
4.877LysLeu: 4.877 ± 0.589
1.289LysMet: 1.289 ± 0.235
2.466LysAsn: 2.466 ± 0.432
2.803LysPro: 2.803 ± 0.529
1.57LysGln: 1.57 ± 0.321
3.195LysArg: 3.195 ± 0.455
2.186LysSer: 2.186 ± 0.351
3.307LysThr: 3.307 ± 0.394
3.7LysVal: 3.7 ± 0.527
1.121LysTrp: 1.121 ± 0.228
0.953LysTyr: 0.953 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
8.184LeuAla: 8.184 ± 0.731
0.448LeuCys: 0.448 ± 0.163
7.007LeuAsp: 7.007 ± 0.558
6.166LeuGlu: 6.166 ± 0.523
2.074LeuPhe: 2.074 ± 0.4
6.054LeuGly: 6.054 ± 0.84
1.794LeuHis: 1.794 ± 0.44
4.596LeuIle: 4.596 ± 0.485
4.709LeuLys: 4.709 ± 0.403
5.717LeuLeu: 5.717 ± 0.547
2.41LeuMet: 2.41 ± 0.351
2.578LeuAsn: 2.578 ± 0.337
3.531LeuPro: 3.531 ± 0.451
2.522LeuGln: 2.522 ± 0.357
5.101LeuArg: 5.101 ± 0.547
4.933LeuSer: 4.933 ± 0.539
4.989LeuThr: 4.989 ± 0.526
4.933LeuVal: 4.933 ± 0.544
1.794LeuTrp: 1.794 ± 0.273
1.121LeuTyr: 1.121 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
2.971MetAla: 2.971 ± 0.337
0.112MetCys: 0.112 ± 0.112
1.345MetAsp: 1.345 ± 0.263
1.513MetGlu: 1.513 ± 0.221
0.448MetPhe: 0.448 ± 0.166
1.289MetGly: 1.289 ± 0.403
0.561MetHis: 0.561 ± 0.177
1.401MetIle: 1.401 ± 0.265
2.186MetLys: 2.186 ± 0.364
1.962MetLeu: 1.962 ± 0.315
0.953MetMet: 0.953 ± 0.234
0.953MetAsn: 0.953 ± 0.237
1.289MetPro: 1.289 ± 0.286
1.065MetGln: 1.065 ± 0.182
1.233MetArg: 1.233 ± 0.312
2.466MetSer: 2.466 ± 0.318
2.354MetThr: 2.354 ± 0.432
1.738MetVal: 1.738 ± 0.305
0.617MetTrp: 0.617 ± 0.216
0.617MetTyr: 0.617 ± 0.15
0.0MetXaa: 0.0 ± 0.0
Asn
3.419AsnAla: 3.419 ± 0.537
0.112AsnCys: 0.112 ± 0.084
2.242AsnAsp: 2.242 ± 0.414
2.41AsnGlu: 2.41 ± 0.344
1.177AsnPhe: 1.177 ± 0.233
3.475AsnGly: 3.475 ± 0.509
0.729AsnHis: 0.729 ± 0.199
1.738AsnIle: 1.738 ± 0.277
1.289AsnLys: 1.289 ± 0.274
3.363AsnLeu: 3.363 ± 0.355
0.617AsnMet: 0.617 ± 0.215
0.897AsnAsn: 0.897 ± 0.192
2.298AsnPro: 2.298 ± 0.432
1.682AsnGln: 1.682 ± 0.316
1.794AsnArg: 1.794 ± 0.259
2.074AsnSer: 2.074 ± 0.297
1.794AsnThr: 1.794 ± 0.327
2.186AsnVal: 2.186 ± 0.387
0.392AsnTrp: 0.392 ± 0.138
0.785AsnTyr: 0.785 ± 0.23
0.0AsnXaa: 0.0 ± 0.0
Pro
3.307ProAla: 3.307 ± 0.501
0.0ProCys: 0.0 ± 0.0
3.812ProAsp: 3.812 ± 0.628
4.877ProGlu: 4.877 ± 0.549
1.121ProPhe: 1.121 ± 0.27
3.924ProGly: 3.924 ± 0.65
1.57ProHis: 1.57 ± 0.354
2.803ProIle: 2.803 ± 0.481
3.139ProLys: 3.139 ± 0.566
2.915ProLeu: 2.915 ± 0.394
1.009ProMet: 1.009 ± 0.223
1.738ProAsn: 1.738 ± 0.284
2.635ProPro: 2.635 ± 0.514
1.289ProGln: 1.289 ± 0.239
2.466ProArg: 2.466 ± 0.474
2.354ProSer: 2.354 ± 0.359
2.242ProThr: 2.242 ± 0.333
3.027ProVal: 3.027 ± 0.38
1.457ProTrp: 1.457 ± 0.222
0.617ProTyr: 0.617 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
3.812GlnAla: 3.812 ± 0.468
0.168GlnCys: 0.168 ± 0.095
2.074GlnAsp: 2.074 ± 0.286
2.859GlnGlu: 2.859 ± 0.378
1.401GlnPhe: 1.401 ± 0.237
2.186GlnGly: 2.186 ± 0.445
0.617GlnHis: 0.617 ± 0.179
2.522GlnIle: 2.522 ± 0.435
2.018GlnLys: 2.018 ± 0.251
3.756GlnLeu: 3.756 ± 0.529
1.121GlnMet: 1.121 ± 0.192
1.233GlnAsn: 1.233 ± 0.247
1.345GlnPro: 1.345 ± 0.251
1.345GlnGln: 1.345 ± 0.234
2.242GlnArg: 2.242 ± 0.409
1.962GlnSer: 1.962 ± 0.288
1.513GlnThr: 1.513 ± 0.268
2.747GlnVal: 2.747 ± 0.454
0.841GlnTrp: 0.841 ± 0.313
0.673GlnTyr: 0.673 ± 0.227
0.0GlnXaa: 0.0 ± 0.0
Arg
5.493ArgAla: 5.493 ± 0.479
0.28ArgCys: 0.28 ± 0.118
4.484ArgAsp: 4.484 ± 0.566
5.717ArgGlu: 5.717 ± 0.678
2.522ArgPhe: 2.522 ± 0.359
3.195ArgGly: 3.195 ± 0.419
1.794ArgHis: 1.794 ± 0.35
4.092ArgIle: 4.092 ± 0.64
3.812ArgLys: 3.812 ± 0.49
4.765ArgLeu: 4.765 ± 0.468
1.906ArgMet: 1.906 ± 0.384
2.074ArgAsn: 2.074 ± 0.352
2.242ArgPro: 2.242 ± 0.462
2.018ArgGln: 2.018 ± 0.282
5.269ArgArg: 5.269 ± 0.887
3.812ArgSer: 3.812 ± 0.436
3.083ArgThr: 3.083 ± 0.47
3.7ArgVal: 3.7 ± 0.366
1.009ArgTrp: 1.009 ± 0.222
1.065ArgTyr: 1.065 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
5.774SerAla: 5.774 ± 0.906
0.336SerCys: 0.336 ± 0.148
3.7SerAsp: 3.7 ± 0.497
4.036SerGlu: 4.036 ± 0.472
1.906SerPhe: 1.906 ± 0.355
5.101SerGly: 5.101 ± 0.658
0.841SerHis: 0.841 ± 0.258
3.027SerIle: 3.027 ± 0.427
3.139SerLys: 3.139 ± 0.408
4.933SerLeu: 4.933 ± 0.583
1.85SerMet: 1.85 ± 0.384
2.074SerAsn: 2.074 ± 0.333
2.803SerPro: 2.803 ± 0.492
2.691SerGln: 2.691 ± 0.35
3.756SerArg: 3.756 ± 0.36
3.027SerSer: 3.027 ± 0.334
3.756SerThr: 3.756 ± 0.502
3.756SerVal: 3.756 ± 0.375
1.121SerTrp: 1.121 ± 0.257
1.233SerTyr: 1.233 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
7.287ThrAla: 7.287 ± 0.962
0.112ThrCys: 0.112 ± 0.079
3.251ThrAsp: 3.251 ± 0.424
4.204ThrGlu: 4.204 ± 0.551
2.298ThrPhe: 2.298 ± 0.339
4.484ThrGly: 4.484 ± 0.675
1.177ThrHis: 1.177 ± 0.245
3.98ThrIle: 3.98 ± 0.478
2.859ThrLys: 2.859 ± 0.468
3.98ThrLeu: 3.98 ± 0.534
1.794ThrMet: 1.794 ± 0.278
2.018ThrAsn: 2.018 ± 0.346
3.587ThrPro: 3.587 ± 0.457
1.682ThrGln: 1.682 ± 0.381
2.803ThrArg: 2.803 ± 0.371
2.859ThrSer: 2.859 ± 0.388
3.924ThrThr: 3.924 ± 0.461
4.204ThrVal: 4.204 ± 0.518
0.953ThrTrp: 0.953 ± 0.204
1.906ThrTyr: 1.906 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
6.839ValAla: 6.839 ± 0.536
0.504ValCys: 0.504 ± 0.19
5.437ValAsp: 5.437 ± 0.653
4.765ValGlu: 4.765 ± 0.564
2.13ValPhe: 2.13 ± 0.283
4.54ValGly: 4.54 ± 0.516
1.401ValHis: 1.401 ± 0.311
3.924ValIle: 3.924 ± 0.444
3.531ValLys: 3.531 ± 0.397
5.101ValLeu: 5.101 ± 0.628
1.513ValMet: 1.513 ± 0.237
2.242ValAsn: 2.242 ± 0.301
2.691ValPro: 2.691 ± 0.369
3.083ValGln: 3.083 ± 0.4
3.924ValArg: 3.924 ± 0.541
3.587ValSer: 3.587 ± 0.419
4.316ValThr: 4.316 ± 0.519
5.157ValVal: 5.157 ± 0.541
1.401ValTrp: 1.401 ± 0.239
1.457ValTyr: 1.457 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
1.401TrpAla: 1.401 ± 0.273
0.28TrpCys: 0.28 ± 0.114
1.233TrpAsp: 1.233 ± 0.313
1.738TrpGlu: 1.738 ± 0.281
0.673TrpPhe: 0.673 ± 0.171
1.009TrpGly: 1.009 ± 0.202
0.168TrpHis: 0.168 ± 0.095
1.626TrpIle: 1.626 ± 0.362
1.009TrpLys: 1.009 ± 0.201
2.186TrpLeu: 2.186 ± 0.364
0.448TrpMet: 0.448 ± 0.136
1.009TrpAsn: 1.009 ± 0.19
0.729TrpPro: 0.729 ± 0.186
0.729TrpGln: 0.729 ± 0.163
1.457TrpArg: 1.457 ± 0.288
1.121TrpSer: 1.121 ± 0.203
1.009TrpThr: 1.009 ± 0.232
1.289TrpVal: 1.289 ± 0.279
0.448TrpTrp: 0.448 ± 0.158
0.504TrpTyr: 0.504 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.018TyrAla: 2.018 ± 0.367
0.056TyrCys: 0.056 ± 0.054
1.289TyrAsp: 1.289 ± 0.303
1.233TyrGlu: 1.233 ± 0.337
0.953TyrPhe: 0.953 ± 0.262
2.074TyrGly: 2.074 ± 0.364
0.504TyrHis: 0.504 ± 0.145
1.401TyrIle: 1.401 ± 0.252
0.953TyrLys: 0.953 ± 0.212
1.626TyrLeu: 1.626 ± 0.352
0.392TyrMet: 0.392 ± 0.135
0.28TyrAsn: 0.28 ± 0.107
1.065TyrPro: 1.065 ± 0.244
0.841TyrGln: 0.841 ± 0.197
1.738TyrArg: 1.738 ± 0.292
1.85TyrSer: 1.85 ± 0.366
1.065TyrThr: 1.065 ± 0.271
1.457TyrVal: 1.457 ± 0.349
0.448TyrTrp: 0.448 ± 0.136
0.561TyrTyr: 0.561 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (17841 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski