Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Spathaspora; Spathaspora passalidarum

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3AGF1|G3AGF1_SPAPN Acyl carrier protein OS=Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1) OX=619300 GN=SPAPADRAFT_58508 PE=3 SV=1
MM1 pKa = 7.24YY2 pKa = 10.63LLLILLQLLTFAATQEE18 pKa = 3.84ISGVFTSFNSLTWLNSNPNTFQTPASPAWIAEE50 pKa = 4.28LSWKK54 pKa = 10.0IDD56 pKa = 3.42GSVVSAGDD64 pKa = 3.72TFSLTMPCVFRR75 pKa = 11.84FYY77 pKa = 10.78TDD79 pKa = 3.05QDD81 pKa = 4.03SVNLRR86 pKa = 11.84VGSTNYY92 pKa = 8.08ATCDD96 pKa = 4.44FITGGISSTDD106 pKa = 3.3SEE108 pKa = 5.06LNCVLSDD115 pKa = 3.69EE116 pKa = 4.5VEE118 pKa = 4.04EE119 pKa = 4.98SILAVGKK126 pKa = 8.69LTFPIVFNIGRR137 pKa = 11.84STEE140 pKa = 4.78DD141 pKa = 2.78VDD143 pKa = 5.68LYY145 pKa = 11.35CSTFYY150 pKa = 10.65QGGLNTISFMDD161 pKa = 4.03GDD163 pKa = 4.08NTLSIDD169 pKa = 4.33VEE171 pKa = 4.48FQDD174 pKa = 4.69GTPSGDD180 pKa = 3.7PNGPMYY186 pKa = 10.25GARR189 pKa = 11.84MLPSSNRR196 pKa = 11.84WQNYY200 pKa = 6.84VLGGLCPGGLSTATIGITINDD221 pKa = 4.16DD222 pKa = 3.16VSTIDD227 pKa = 5.04CEE229 pKa = 4.27TANIRR234 pKa = 11.84LTNAVNDD241 pKa = 3.65WFYY244 pKa = 11.03PEE246 pKa = 4.13SFKK249 pKa = 10.98TFTTASKK256 pKa = 10.56LCRR259 pKa = 11.84SNSFMITYY267 pKa = 9.33WGVPDD272 pKa = 4.67GYY274 pKa = 10.65RR275 pKa = 11.84VFIEE279 pKa = 4.42AVINTDD285 pKa = 3.26GNPPRR290 pKa = 11.84VQYY293 pKa = 8.82TNKK296 pKa = 9.55YY297 pKa = 9.15LCPTTIDD304 pKa = 3.11NGVWVNWLPYY314 pKa = 10.61DD315 pKa = 3.82SVEE318 pKa = 3.95ADD320 pKa = 3.56GPGKK324 pKa = 10.38EE325 pKa = 4.35IITVTEE331 pKa = 3.92TWTGSDD337 pKa = 3.43TEE339 pKa = 4.77TITKK343 pKa = 10.43SFDD346 pKa = 3.19EE347 pKa = 5.6DD348 pKa = 3.5EE349 pKa = 5.14DD350 pKa = 3.89LTITIAVNIPIPTITLTEE368 pKa = 4.33TYY370 pKa = 10.86VGITTSYY377 pKa = 6.95TTYY380 pKa = 9.58TVEE383 pKa = 4.3PGEE386 pKa = 4.24TASVVVYY393 pKa = 10.51DD394 pKa = 4.68PVHH397 pKa = 4.66TTITEE402 pKa = 4.11EE403 pKa = 4.33SCWEE407 pKa = 3.89SDD409 pKa = 3.28EE410 pKa = 5.94SITTTIIDD418 pKa = 4.58PDD420 pKa = 3.79WASDD424 pKa = 3.64TILIVTPCEE433 pKa = 4.12VTSTEE438 pKa = 4.09QEE440 pKa = 3.92EE441 pKa = 4.93SSTKK445 pKa = 10.37VEE447 pKa = 5.54DD448 pKa = 3.74IPSDD452 pKa = 3.59DD453 pKa = 4.05TLDD456 pKa = 4.25DD457 pKa = 4.48DD458 pKa = 5.46SYY460 pKa = 11.7NPPEE464 pKa = 4.17IADD467 pKa = 3.62NASSEE472 pKa = 4.5EE473 pKa = 4.07ITSGEE478 pKa = 4.18VQSTEE483 pKa = 3.75ILSSEE488 pKa = 4.29EE489 pKa = 4.03VQSTEE494 pKa = 3.73ILSSEE499 pKa = 4.29EE500 pKa = 4.03VQSTEE505 pKa = 3.8ILSSEE510 pKa = 4.11EE511 pKa = 4.38SFSQDD516 pKa = 2.55STTVPYY522 pKa = 10.89GSTKK526 pKa = 7.91EE527 pKa = 4.31TEE529 pKa = 3.7ISTEE533 pKa = 3.69IVLEE537 pKa = 4.3EE538 pKa = 4.23TFSTEE543 pKa = 4.15DD544 pKa = 3.25TTPEE548 pKa = 3.74NNTWDD553 pKa = 4.1GLTDD557 pKa = 5.09DD558 pKa = 6.06DD559 pKa = 4.79SQQPSSNTKK568 pKa = 8.79NDD570 pKa = 3.39SNEE573 pKa = 4.28EE574 pKa = 4.08YY575 pKa = 10.4TSDD578 pKa = 4.12QIDD581 pKa = 3.41ISSSGSEE588 pKa = 3.86TSAKK592 pKa = 8.72QTTTEE597 pKa = 4.53TIPDD601 pKa = 3.42NHH603 pKa = 6.29TTDD606 pKa = 4.0IVQEE610 pKa = 4.21DD611 pKa = 4.52PGTEE615 pKa = 3.64ILSVEE620 pKa = 4.19NTTVRR625 pKa = 11.84VPDD628 pKa = 4.85DD629 pKa = 3.67NTTDD633 pKa = 3.27ISPSDD638 pKa = 3.85VICNSEE644 pKa = 4.1NEE646 pKa = 4.33ASSEE650 pKa = 4.01PNEE653 pKa = 3.71TRR655 pKa = 11.84TYY657 pKa = 10.59VSIVPTTKK665 pKa = 10.63SKK667 pKa = 11.36DD668 pKa = 3.5STITSAFSGYY678 pKa = 9.74EE679 pKa = 3.27QDD681 pKa = 4.15TITKK685 pKa = 9.5VITYY689 pKa = 7.28TTTICEE695 pKa = 4.51SDD697 pKa = 3.46TVTQVITYY705 pKa = 6.74TTIITNHH712 pKa = 6.63DD713 pKa = 4.59SIAKK717 pKa = 7.96TATEE721 pKa = 4.23MTTEE725 pKa = 3.99ATSFAVTTVGEE736 pKa = 4.34TRR738 pKa = 11.84TIIYY742 pKa = 9.54LQDD745 pKa = 3.32SMGTTNDD752 pKa = 3.15TGNNSSNTKK761 pKa = 9.86SDD763 pKa = 2.98SRR765 pKa = 11.84TASNNTGNNSSNTKK779 pKa = 9.92SYY781 pKa = 11.19SGTASNEE788 pKa = 4.14EE789 pKa = 3.93IQTTANSEE797 pKa = 4.18NNSEE801 pKa = 4.22HH802 pKa = 6.07TKK804 pKa = 10.56SEE806 pKa = 4.14SGQSDD811 pKa = 2.61ISEE814 pKa = 4.49YY815 pKa = 10.86YY816 pKa = 10.52SGGTEE821 pKa = 3.9SDD823 pKa = 3.19SGKK826 pKa = 10.47GNKK829 pKa = 9.52SVVPEE834 pKa = 4.16EE835 pKa = 4.09NQGTNSRR842 pKa = 11.84NNNSSTNSNNRR853 pKa = 11.84SDD855 pKa = 4.41DD856 pKa = 3.26QTTRR860 pKa = 11.84GSYY863 pKa = 8.49STSLQLVTSNGIGSTRR879 pKa = 11.84GTSTFSLLFTFVIAILYY896 pKa = 8.94

Molecular weight: 97.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3ATQ7|G3ATQ7_SPAPN WD_REPEATS_REGION domain-containing protein OS=Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1) OX=619300 GN=SPAPADRAFT_52383 PE=3 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 9.21QNRR21 pKa = 11.84PLPQWVRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.88LGII51 pKa = 4.0

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5973

0

5973

2693134

49

4210

450.9

50.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.585 ± 0.028

1.11 ± 0.01

5.896 ± 0.025

6.759 ± 0.039

4.453 ± 0.019

5.219 ± 0.033

2.212 ± 0.014

6.926 ± 0.022

6.96 ± 0.033

9.351 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.011

5.599 ± 0.025

4.68 ± 0.028

4.269 ± 0.032

4.115 ± 0.021

8.434 ± 0.032

5.96 ± 0.034

5.929 ± 0.024

1.025 ± 0.01

3.617 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski