Cohaesibacter sp. CAU 1516

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Cohaesibacteraceae; Cohaesibacter; unclassified Cohaesibacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R8YG09|A0A5R8YG09_9RHIZ LysR family transcriptional regulator OS=Cohaesibacter sp. CAU 1516 OX=2576038 GN=FDK21_06130 PE=3 SV=1
MM1 pKa = 7.38CTLCGIGLPDD11 pKa = 3.36FHH13 pKa = 6.98GQTFTPLSGSEE24 pKa = 4.11SGDD27 pKa = 3.24GLTASTASVSDD38 pKa = 4.04PGEE41 pKa = 3.78VWKK44 pKa = 10.93GVLHH48 pKa = 5.34DD49 pKa = 4.23TKK51 pKa = 10.32WAVSSLQFSFPTSASFYY68 pKa = 9.1ASTYY72 pKa = 10.57GSEE75 pKa = 4.16QPDD78 pKa = 3.64LGFEE82 pKa = 4.38ALNSVQKK89 pKa = 10.81DD90 pKa = 3.7AVRR93 pKa = 11.84SLYY96 pKa = 10.94NEE98 pKa = 3.93IEE100 pKa = 4.35SFTDD104 pKa = 3.34LTFTEE109 pKa = 4.69VTEE112 pKa = 4.46SSTTHH117 pKa = 6.11AEE119 pKa = 3.69LRR121 pKa = 11.84FAMTLNNTPTAAGYY135 pKa = 9.45FPYY138 pKa = 10.74GSDD141 pKa = 3.32EE142 pKa = 5.84AGDD145 pKa = 3.04SWYY148 pKa = 10.9RR149 pKa = 11.84NDD151 pKa = 5.02GNYY154 pKa = 10.4DD155 pKa = 3.5DD156 pKa = 5.42PRR158 pKa = 11.84LGSYY162 pKa = 10.22AYY164 pKa = 10.61NEE166 pKa = 4.26GFMHH170 pKa = 7.38EE171 pKa = 4.27IGHH174 pKa = 5.52TLGLTHH180 pKa = 6.53GHH182 pKa = 7.27DD183 pKa = 3.53SGNPFGALPSNVDD196 pKa = 3.55SNEE199 pKa = 3.78FSVMTYY205 pKa = 9.61RR206 pKa = 11.84DD207 pKa = 3.54HH208 pKa = 9.04VGDD211 pKa = 4.07SFTDD215 pKa = 3.84PSEE218 pKa = 4.22NEE220 pKa = 3.7IGGNAQSYY228 pKa = 8.41MMYY231 pKa = 10.38DD232 pKa = 3.56IAALQYY238 pKa = 9.99MYY240 pKa = 10.93GADD243 pKa = 3.61YY244 pKa = 10.71SASGNVWSGDD254 pKa = 3.23TVYY257 pKa = 10.79TFDD260 pKa = 4.44PSSGQMSINGAGQGTPVEE278 pKa = 3.97NRR280 pKa = 11.84IFRR283 pKa = 11.84TIWDD287 pKa = 3.81GNGVDD292 pKa = 4.87TYY294 pKa = 11.69DD295 pKa = 3.5LSNYY299 pKa = 7.11TTDD302 pKa = 5.02LSINLNPGEE311 pKa = 3.99WSIFSQAQLADD322 pKa = 3.99LNGGSRR328 pKa = 11.84SDD330 pKa = 3.42YY331 pKa = 10.33LARR334 pKa = 11.84GNVANALLFEE344 pKa = 5.07GDD346 pKa = 3.68LRR348 pKa = 11.84SLIEE352 pKa = 3.89NANGGRR358 pKa = 11.84GNDD361 pKa = 3.89TIIGNQGSNEE371 pKa = 3.63LRR373 pKa = 11.84GNGGNDD379 pKa = 3.02NLQGGDD385 pKa = 3.58GRR387 pKa = 11.84DD388 pKa = 3.11KK389 pKa = 11.05AIYY392 pKa = 9.35SGAYY396 pKa = 8.81SEE398 pKa = 4.75YY399 pKa = 10.72SITEE403 pKa = 4.33NIDD406 pKa = 3.05GSFTIAHH413 pKa = 6.24TGGDD417 pKa = 3.66RR418 pKa = 11.84SDD420 pKa = 3.64GTDD423 pKa = 2.88TVRR426 pKa = 11.84DD427 pKa = 3.03IEE429 pKa = 4.37YY430 pKa = 10.61AVFSDD435 pKa = 4.76RR436 pKa = 11.84EE437 pKa = 4.06VALAPLTGPLDD448 pKa = 3.76VVFLQDD454 pKa = 3.68LTASFIDD461 pKa = 4.56DD462 pKa = 4.3LPSMRR467 pKa = 11.84AAVDD471 pKa = 4.4DD472 pKa = 3.94IAAVIQNAYY481 pKa = 9.75ANSRR485 pKa = 11.84FAVTSFRR492 pKa = 11.84DD493 pKa = 3.29TGDD496 pKa = 3.25SYY498 pKa = 11.57VYY500 pKa = 10.16RR501 pKa = 11.84VEE503 pKa = 5.22ADD505 pKa = 3.68FTQSTSSLVSTYY517 pKa = 11.25NSMNATGGGDD527 pKa = 3.36TPEE530 pKa = 4.32AQLTALLQASTDD542 pKa = 3.32SSLSYY547 pKa = 10.7QSGSTRR553 pKa = 11.84IFILATDD560 pKa = 4.2AGYY563 pKa = 10.87HH564 pKa = 5.52NYY566 pKa = 10.38EE567 pKa = 4.68SIASIAALMASNDD580 pKa = 3.99IIPIFAVASGEE591 pKa = 4.03VSTYY595 pKa = 10.89QNLANEE601 pKa = 4.92LGTGVVVTIEE611 pKa = 3.99SDD613 pKa = 3.51SADD616 pKa = 3.42FADD619 pKa = 4.41SIRR622 pKa = 11.84YY623 pKa = 8.74ALASIDD629 pKa = 4.27AEE631 pKa = 4.29VTEE634 pKa = 5.34AGTSSGDD641 pKa = 3.39TLAGGDD647 pKa = 3.98TADD650 pKa = 4.69GIYY653 pKa = 10.89GLGGDD658 pKa = 4.55DD659 pKa = 3.95TISGNGGDD667 pKa = 4.76DD668 pKa = 3.98LLDD671 pKa = 4.19GGSGNDD677 pKa = 3.55SLLGGDD683 pKa = 5.79DD684 pKa = 3.77NDD686 pKa = 3.7QLLGGSGNDD695 pKa = 3.19NLLGGEE701 pKa = 4.31GNDD704 pKa = 3.41RR705 pKa = 11.84LVGGDD710 pKa = 3.79GDD712 pKa = 5.31DD713 pKa = 3.72VLLGDD718 pKa = 4.83GDD720 pKa = 4.19SSSSLFPSSLSLNSLFTLGFDD741 pKa = 4.28EE742 pKa = 6.54DD743 pKa = 4.02IQNSTTQAHH752 pKa = 5.82ATVQGTGSGSVNYY765 pKa = 10.4YY766 pKa = 10.38SFTVAAAGLSVVLDD780 pKa = 3.6IDD782 pKa = 4.36YY783 pKa = 11.27ADD785 pKa = 3.79QNIGASDD792 pKa = 3.87FDD794 pKa = 3.81SWIVLYY800 pKa = 10.66DD801 pKa = 3.35SSGNQLSYY809 pKa = 11.55SDD811 pKa = 5.12DD812 pKa = 4.35DD813 pKa = 4.36YY814 pKa = 12.03GGDD817 pKa = 3.58PGSVDD822 pKa = 2.98SHH824 pKa = 7.41DD825 pKa = 4.53SYY827 pKa = 11.7LSHH830 pKa = 6.96TLGSAGTYY838 pKa = 8.34YY839 pKa = 10.81VAVGSYY845 pKa = 10.72SSLSPIPVGGTYY857 pKa = 9.85EE858 pKa = 3.96LHH860 pKa = 6.29VSVNGAIGEE869 pKa = 4.29PPTVGAGNDD878 pKa = 3.52VLNGGEE884 pKa = 4.8GADD887 pKa = 3.91SMAGGAGNDD896 pKa = 3.23VYY898 pKa = 11.62YY899 pKa = 10.71VDD901 pKa = 4.66NVGDD905 pKa = 4.13TIEE908 pKa = 4.31EE909 pKa = 4.1NADD912 pKa = 3.39NGNDD916 pKa = 3.5LVFSYY921 pKa = 10.8ISLALRR927 pKa = 11.84DD928 pKa = 3.65HH929 pKa = 6.07SQNLEE934 pKa = 3.99HH935 pKa = 7.29LSLLGPGNINGTGNGLGNTITGNSGNNVLNGAYY968 pKa = 10.32GNDD971 pKa = 3.54VLRR974 pKa = 11.84GGAGNDD980 pKa = 2.97IFKK983 pKa = 10.82DD984 pKa = 3.59DD985 pKa = 3.65VGADD989 pKa = 3.5RR990 pKa = 11.84MIGGSGNDD998 pKa = 3.14VYY1000 pKa = 11.67YY1001 pKa = 10.74VDD1003 pKa = 4.18NVGDD1007 pKa = 3.81RR1008 pKa = 11.84VEE1010 pKa = 4.31EE1011 pKa = 4.29SAGSGTDD1018 pKa = 3.1QVFSYY1023 pKa = 10.89ISFALRR1029 pKa = 11.84DD1030 pKa = 3.43HH1031 pKa = 5.98SQYY1034 pKa = 11.19LEE1036 pKa = 4.02HH1037 pKa = 7.28LSLLGSGNTNGTGNAQHH1054 pKa = 5.94NTITGNSGNNILNGAGGNDD1073 pKa = 3.45ILRR1076 pKa = 11.84GGAGNDD1082 pKa = 2.91IFKK1085 pKa = 11.1DD1086 pKa = 3.56NIGADD1091 pKa = 3.49RR1092 pKa = 11.84MIGGTGNDD1100 pKa = 3.08VYY1102 pKa = 11.54YY1103 pKa = 10.84VDD1105 pKa = 4.03NGGDD1109 pKa = 3.35RR1110 pKa = 11.84VEE1112 pKa = 4.5EE1113 pKa = 4.28SAGSGTDD1120 pKa = 3.1QVFSYY1125 pKa = 10.89ISFALRR1131 pKa = 11.84DD1132 pKa = 3.43HH1133 pKa = 5.98SQYY1136 pKa = 11.19LEE1138 pKa = 4.02HH1139 pKa = 7.28LSLLGSGDD1147 pKa = 3.36INGTGNGLGNTITGNAGNNVLNGAYY1172 pKa = 10.33GNDD1175 pKa = 3.89LLRR1178 pKa = 11.84GGAGNDD1184 pKa = 2.99IFKK1187 pKa = 10.6DD1188 pKa = 3.6DD1189 pKa = 3.9NGADD1193 pKa = 3.51RR1194 pKa = 11.84MIGGTGNDD1202 pKa = 3.14VYY1204 pKa = 11.59YY1205 pKa = 10.67VDD1207 pKa = 4.18NVGDD1211 pKa = 3.81RR1212 pKa = 11.84VEE1214 pKa = 4.31EE1215 pKa = 4.29SAGSGTDD1222 pKa = 3.11QVFSYY1227 pKa = 11.57VSFTLRR1233 pKa = 11.84NHH1235 pKa = 5.25SQYY1238 pKa = 11.15LEE1240 pKa = 4.06HH1241 pKa = 7.32LSLLGAGNINGTGNGLNNIITGNSGNNVLNGAYY1274 pKa = 10.05GQDD1277 pKa = 3.29ILRR1280 pKa = 11.84GGAGNDD1286 pKa = 2.87IFFDD1290 pKa = 4.39DD1291 pKa = 4.24SGNDD1295 pKa = 2.82IFTGGTGADD1304 pKa = 3.11TFIFQRR1310 pKa = 11.84SGEE1313 pKa = 3.99FDD1315 pKa = 3.81IITDD1319 pKa = 4.24FEE1321 pKa = 5.29DD1322 pKa = 5.41GIDD1325 pKa = 3.86TIRR1328 pKa = 11.84IATGSNTFSQVKK1340 pKa = 10.12ISDD1343 pKa = 4.21DD1344 pKa = 3.71GADD1347 pKa = 3.76TIVQFDD1353 pKa = 3.88GSTVVLNNFNHH1364 pKa = 6.4TLLGADD1370 pKa = 4.08DD1371 pKa = 4.45FSFVV1375 pKa = 3.42

Molecular weight: 143.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R8YD88|A0A5R8YD88_9RHIZ Uncharacterized protein OS=Cohaesibacter sp. CAU 1516 OX=2576038 GN=FDK21_00855 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3998

0

3998

1310659

24

2583

327.8

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.855 ± 0.044

0.966 ± 0.012

6.099 ± 0.037

5.916 ± 0.038

3.947 ± 0.025

7.961 ± 0.038

2.17 ± 0.02

5.9 ± 0.029

4.37 ± 0.031

10.247 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 0.02

3.063 ± 0.023

4.602 ± 0.03

3.528 ± 0.025

5.806 ± 0.037

6.017 ± 0.033

5.164 ± 0.03

6.911 ± 0.032

1.245 ± 0.014

2.36 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski