Amino acid dipepetide frequency for Arthrobacter sp. Bz4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.295AlaAla: 18.295 ± 0.219
0.735AlaCys: 0.735 ± 0.029
7.38AlaAsp: 7.38 ± 0.093
7.556AlaGlu: 7.556 ± 0.103
3.529AlaPhe: 3.529 ± 0.069
11.585AlaGly: 11.585 ± 0.141
2.315AlaHis: 2.315 ± 0.055
5.188AlaIle: 5.188 ± 0.077
3.036AlaLys: 3.036 ± 0.067
12.772AlaLeu: 12.772 ± 0.137
2.654AlaMet: 2.654 ± 0.059
2.604AlaAsn: 2.604 ± 0.053
5.657AlaPro: 5.657 ± 0.088
3.966AlaGln: 3.966 ± 0.07
7.479AlaArg: 7.479 ± 0.103
6.987AlaSer: 6.987 ± 0.091
6.613AlaThr: 6.613 ± 0.083
11.093AlaVal: 11.093 ± 0.146
1.502AlaTrp: 1.502 ± 0.039
2.156AlaTyr: 2.156 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.633CysAla: 0.633 ± 0.026
0.057CysCys: 0.057 ± 0.008
0.369CysAsp: 0.369 ± 0.021
0.305CysGlu: 0.305 ± 0.021
0.177CysPhe: 0.177 ± 0.014
0.681CysGly: 0.681 ± 0.029
0.139CysHis: 0.139 ± 0.013
0.238CysIle: 0.238 ± 0.017
0.107CysLys: 0.107 ± 0.011
0.565CysLeu: 0.565 ± 0.024
0.103CysMet: 0.103 ± 0.01
0.133CysAsn: 0.133 ± 0.011
0.392CysPro: 0.392 ± 0.023
0.163CysGln: 0.163 ± 0.013
0.46CysArg: 0.46 ± 0.024
0.423CysSer: 0.423 ± 0.025
0.399CysThr: 0.399 ± 0.02
0.434CysVal: 0.434 ± 0.025
0.095CysTrp: 0.095 ± 0.011
0.127CysTyr: 0.127 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.152AspAla: 7.152 ± 0.105
0.315AspCys: 0.315 ± 0.022
3.925AspAsp: 3.925 ± 0.078
3.794AspGlu: 3.794 ± 0.085
1.864AspPhe: 1.864 ± 0.047
5.744AspGly: 5.744 ± 0.085
1.321AspHis: 1.321 ± 0.037
2.503AspIle: 2.503 ± 0.054
1.243AspLys: 1.243 ± 0.04
6.292AspLeu: 6.292 ± 0.082
0.877AspMet: 0.877 ± 0.03
1.261AspAsn: 1.261 ± 0.04
3.886AspPro: 3.886 ± 0.076
2.053AspGln: 2.053 ± 0.047
3.936AspArg: 3.936 ± 0.064
3.133AspSer: 3.133 ± 0.059
3.035AspThr: 3.035 ± 0.062
4.901AspVal: 4.901 ± 0.077
0.892AspTrp: 0.892 ± 0.03
1.335AspTyr: 1.335 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.807GluAla: 6.807 ± 0.111
0.285GluCys: 0.285 ± 0.017
3.055GluAsp: 3.055 ± 0.064
3.336GluGlu: 3.336 ± 0.083
1.723GluPhe: 1.723 ± 0.043
4.072GluGly: 4.072 ± 0.073
1.507GluHis: 1.507 ± 0.04
2.747GluIle: 2.747 ± 0.052
1.586GluLys: 1.586 ± 0.051
6.684GluLeu: 6.684 ± 0.104
1.009GluMet: 1.009 ± 0.037
1.494GluAsn: 1.494 ± 0.048
2.967GluPro: 2.967 ± 0.066
2.388GluGln: 2.388 ± 0.059
4.284GluArg: 4.284 ± 0.078
3.272GluSer: 3.272 ± 0.066
3.263GluThr: 3.263 ± 0.062
4.507GluVal: 4.507 ± 0.075
0.751GluTrp: 0.751 ± 0.027
1.126GluTyr: 1.126 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.844PheAla: 3.844 ± 0.073
0.247PheCys: 0.247 ± 0.018
2.266PheAsp: 2.266 ± 0.044
1.812PheGlu: 1.812 ± 0.045
1.157PhePhe: 1.157 ± 0.04
3.311PheGly: 3.311 ± 0.056
0.64PheHis: 0.64 ± 0.028
1.434PheIle: 1.434 ± 0.044
0.674PheLys: 0.674 ± 0.031
3.027PheLeu: 3.027 ± 0.057
0.553PheMet: 0.553 ± 0.025
0.906PheAsn: 0.906 ± 0.033
1.422PhePro: 1.422 ± 0.041
0.824PheGln: 0.824 ± 0.027
1.771PheArg: 1.771 ± 0.045
2.052PheSer: 2.052 ± 0.048
2.265PheThr: 2.265 ± 0.047
2.527PheVal: 2.527 ± 0.052
0.494PheTrp: 0.494 ± 0.023
0.682PheTyr: 0.682 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
9.695GlyAla: 9.695 ± 0.128
0.666GlyCys: 0.666 ± 0.03
4.618GlyAsp: 4.618 ± 0.077
4.522GlyGlu: 4.522 ± 0.078
3.219GlyPhe: 3.219 ± 0.06
7.257GlyGly: 7.257 ± 0.111
1.88GlyHis: 1.88 ± 0.041
4.651GlyIle: 4.651 ± 0.073
2.652GlyLys: 2.652 ± 0.067
8.939GlyLeu: 8.939 ± 0.11
2.037GlyMet: 2.037 ± 0.05
2.156GlyAsn: 2.156 ± 0.048
4.012GlyPro: 4.012 ± 0.068
2.908GlyGln: 2.908 ± 0.058
5.724GlyArg: 5.724 ± 0.085
5.905GlySer: 5.905 ± 0.088
5.737GlyThr: 5.737 ± 0.084
7.359GlyVal: 7.359 ± 0.094
1.588GlyTrp: 1.588 ± 0.044
2.292GlyTyr: 2.292 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.242HisAla: 2.242 ± 0.053
0.155HisCys: 0.155 ± 0.011
1.206HisAsp: 1.206 ± 0.03
1.05HisGlu: 1.05 ± 0.035
0.649HisPhe: 0.649 ± 0.026
1.935HisGly: 1.935 ± 0.052
0.684HisHis: 0.684 ± 0.032
0.805HisIle: 0.805 ± 0.028
0.389HisLys: 0.389 ± 0.02
2.392HisLeu: 2.392 ± 0.048
0.317HisMet: 0.317 ± 0.019
0.502HisAsn: 0.502 ± 0.023
1.494HisPro: 1.494 ± 0.042
0.8HisGln: 0.8 ± 0.03
1.744HisArg: 1.744 ± 0.05
1.275HisSer: 1.275 ± 0.035
1.241HisThr: 1.241 ± 0.038
1.406HisVal: 1.406 ± 0.043
0.308HisTrp: 0.308 ± 0.017
0.498HisTyr: 0.498 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.035IleAla: 6.035 ± 0.081
0.323IleCys: 0.323 ± 0.017
3.225IleAsp: 3.225 ± 0.066
2.697IleGlu: 2.697 ± 0.048
1.446IlePhe: 1.446 ± 0.048
4.393IleGly: 4.393 ± 0.077
0.875IleHis: 0.875 ± 0.03
2.153IleIle: 2.153 ± 0.058
1.093IleLys: 1.093 ± 0.035
4.362IleLeu: 4.362 ± 0.073
0.759IleMet: 0.759 ± 0.034
1.303IleAsn: 1.303 ± 0.039
2.464IlePro: 2.464 ± 0.06
1.269IleGln: 1.269 ± 0.035
2.861IleArg: 2.861 ± 0.051
2.87IleSer: 2.87 ± 0.065
2.935IleThr: 2.935 ± 0.06
3.674IleVal: 3.674 ± 0.065
0.552IleTrp: 0.552 ± 0.027
0.875IleTyr: 0.875 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
2.961LysAla: 2.961 ± 0.07
0.102LysCys: 0.102 ± 0.01
1.422LysAsp: 1.422 ± 0.043
1.267LysGlu: 1.267 ± 0.044
0.665LysPhe: 0.665 ± 0.03
1.919LysGly: 1.919 ± 0.05
0.543LysHis: 0.543 ± 0.027
1.188LysIle: 1.188 ± 0.037
0.9LysLys: 0.9 ± 0.039
2.371LysLeu: 2.371 ± 0.051
0.529LysMet: 0.529 ± 0.025
0.696LysAsn: 0.696 ± 0.032
1.501LysPro: 1.501 ± 0.046
0.799LysGln: 0.799 ± 0.032
1.694LysArg: 1.694 ± 0.048
1.559LysSer: 1.559 ± 0.049
1.549LysThr: 1.549 ± 0.04
2.019LysVal: 2.019 ± 0.048
0.298LysTrp: 0.298 ± 0.017
0.552LysTyr: 0.552 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
13.83LeuAla: 13.83 ± 0.158
0.597LeuCys: 0.597 ± 0.025
6.337LeuAsp: 6.337 ± 0.092
5.624LeuGlu: 5.624 ± 0.087
3.073LeuPhe: 3.073 ± 0.059
9.073LeuGly: 9.073 ± 0.107
2.03LeuHis: 2.03 ± 0.054
4.99LeuIle: 4.99 ± 0.082
2.439LeuLys: 2.439 ± 0.057
10.751LeuLeu: 10.751 ± 0.136
1.987LeuMet: 1.987 ± 0.042
2.521LeuAsn: 2.521 ± 0.048
5.755LeuPro: 5.755 ± 0.086
3.139LeuGln: 3.139 ± 0.061
7.176LeuArg: 7.176 ± 0.104
6.365LeuSer: 6.365 ± 0.089
6.829LeuThr: 6.829 ± 0.085
8.818LeuVal: 8.818 ± 0.116
1.256LeuTrp: 1.256 ± 0.047
1.788LeuTyr: 1.788 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.426MetAla: 2.426 ± 0.056
0.11MetCys: 0.11 ± 0.01
1.034MetAsp: 1.034 ± 0.035
0.878MetGlu: 0.878 ± 0.033
0.576MetPhe: 0.576 ± 0.026
1.58MetGly: 1.58 ± 0.043
0.363MetHis: 0.363 ± 0.023
1.019MetIle: 1.019 ± 0.038
0.604MetLys: 0.604 ± 0.027
1.923MetLeu: 1.923 ± 0.057
0.399MetMet: 0.399 ± 0.021
0.595MetAsn: 0.595 ± 0.025
1.08MetPro: 1.08 ± 0.034
0.559MetGln: 0.559 ± 0.023
1.23MetArg: 1.23 ± 0.037
1.468MetSer: 1.468 ± 0.041
1.633MetThr: 1.633 ± 0.042
1.665MetVal: 1.665 ± 0.04
0.2MetTrp: 0.2 ± 0.015
0.315MetTyr: 0.315 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.693AsnAla: 2.693 ± 0.059
0.147AsnCys: 0.147 ± 0.011
1.368AsnAsp: 1.368 ± 0.044
1.27AsnGlu: 1.27 ± 0.036
0.792AsnPhe: 0.792 ± 0.029
2.225AsnGly: 2.225 ± 0.046
0.584AsnHis: 0.584 ± 0.027
1.117AsnIle: 1.117 ± 0.036
0.573AsnLys: 0.573 ± 0.027
2.457AsnLeu: 2.457 ± 0.047
0.399AsnMet: 0.399 ± 0.022
0.702AsnAsn: 0.702 ± 0.029
1.873AsnPro: 1.873 ± 0.043
0.842AsnGln: 0.842 ± 0.03
1.7AsnArg: 1.7 ± 0.043
1.45AsnSer: 1.45 ± 0.043
1.345AsnThr: 1.345 ± 0.038
1.847AsnVal: 1.847 ± 0.047
0.404AsnTrp: 0.404 ± 0.019
0.6AsnTyr: 0.6 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
7.114ProAla: 7.114 ± 0.096
0.22ProCys: 0.22 ± 0.017
3.728ProAsp: 3.728 ± 0.068
3.971ProGlu: 3.971 ± 0.069
1.673ProPhe: 1.673 ± 0.04
5.231ProGly: 5.231 ± 0.089
1.11ProHis: 1.11 ± 0.033
1.971ProIle: 1.971 ± 0.045
1.21ProLys: 1.21 ± 0.043
4.906ProLeu: 4.906 ± 0.081
1.032ProMet: 1.032 ± 0.033
1.155ProAsn: 1.155 ± 0.033
2.399ProPro: 2.399 ± 0.068
1.655ProGln: 1.655 ± 0.045
2.986ProArg: 2.986 ± 0.058
3.297ProSer: 3.297 ± 0.063
3.275ProThr: 3.275 ± 0.063
4.746ProVal: 4.746 ± 0.073
0.804ProTrp: 0.804 ± 0.028
0.96ProTyr: 0.96 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.758GlnAla: 3.758 ± 0.07
0.156GlnCys: 0.156 ± 0.012
1.709GlnAsp: 1.709 ± 0.045
1.744GlnGlu: 1.744 ± 0.048
0.969GlnPhe: 0.969 ± 0.033
2.369GlnGly: 2.369 ± 0.049
0.755GlnHis: 0.755 ± 0.028
1.615GlnIle: 1.615 ± 0.045
0.768GlnLys: 0.768 ± 0.033
4.077GlnLeu: 4.077 ± 0.073
0.67GlnMet: 0.67 ± 0.031
0.703GlnAsn: 0.703 ± 0.027
1.82GlnPro: 1.82 ± 0.042
1.494GlnGln: 1.494 ± 0.045
2.582GlnArg: 2.582 ± 0.055
1.761GlnSer: 1.761 ± 0.043
1.789GlnThr: 1.789 ± 0.047
2.605GlnVal: 2.605 ± 0.055
0.592GlnTrp: 0.592 ± 0.026
0.633GlnTyr: 0.633 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
6.926ArgAla: 6.926 ± 0.099
0.399ArgCys: 0.399 ± 0.02
3.645ArgAsp: 3.645 ± 0.067
3.821ArgGlu: 3.821 ± 0.064
2.406ArgPhe: 2.406 ± 0.049
4.662ArgGly: 4.662 ± 0.066
1.532ArgHis: 1.532 ± 0.042
3.427ArgIle: 3.427 ± 0.054
1.859ArgLys: 1.859 ± 0.047
7.174ArgLeu: 7.174 ± 0.11
1.572ArgMet: 1.572 ± 0.037
1.757ArgAsn: 1.757 ± 0.043
3.371ArgPro: 3.371 ± 0.07
2.375ArgGln: 2.375 ± 0.045
5.721ArgArg: 5.721 ± 0.105
4.409ArgSer: 4.409 ± 0.064
4.135ArgThr: 4.135 ± 0.067
5.132ArgVal: 5.132 ± 0.088
1.141ArgTrp: 1.141 ± 0.039
1.535ArgTyr: 1.535 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.245SerAla: 7.245 ± 0.087
0.363SerCys: 0.363 ± 0.02
3.252SerAsp: 3.252 ± 0.066
3.216SerGlu: 3.216 ± 0.071
2.053SerPhe: 2.053 ± 0.051
6.065SerGly: 6.065 ± 0.088
1.202SerHis: 1.202 ± 0.036
2.682SerIle: 2.682 ± 0.051
1.417SerLys: 1.417 ± 0.039
6.157SerLeu: 6.157 ± 0.083
1.394SerMet: 1.394 ± 0.04
1.457SerAsn: 1.457 ± 0.045
3.398SerPro: 3.398 ± 0.072
1.907SerGln: 1.907 ± 0.046
4.092SerArg: 4.092 ± 0.063
4.042SerSer: 4.042 ± 0.07
4.057SerThr: 4.057 ± 0.066
5.101SerVal: 5.101 ± 0.08
0.937SerTrp: 0.937 ± 0.032
1.375SerTyr: 1.375 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
7.65ThrAla: 7.65 ± 0.101
0.335ThrCys: 0.335 ± 0.019
3.522ThrAsp: 3.522 ± 0.064
3.314ThrGlu: 3.314 ± 0.062
1.912ThrPhe: 1.912 ± 0.049
6.136ThrGly: 6.136 ± 0.086
1.202ThrHis: 1.202 ± 0.037
2.859ThrIle: 2.859 ± 0.062
1.406ThrLys: 1.406 ± 0.033
6.069ThrLeu: 6.069 ± 0.085
1.105ThrMet: 1.105 ± 0.036
1.388ThrAsn: 1.388 ± 0.04
3.791ThrPro: 3.791 ± 0.072
1.635ThrGln: 1.635 ± 0.044
3.52ThrArg: 3.52 ± 0.055
3.772ThrSer: 3.772 ± 0.06
3.856ThrThr: 3.856 ± 0.069
5.73ThrVal: 5.73 ± 0.094
0.716ThrTrp: 0.716 ± 0.027
1.296ThrTyr: 1.296 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.072ValAla: 10.072 ± 0.11
0.535ValCys: 0.535 ± 0.023
5.308ValAsp: 5.308 ± 0.083
4.896ValGlu: 4.896 ± 0.072
2.706ValPhe: 2.706 ± 0.058
6.716ValGly: 6.716 ± 0.093
1.71ValHis: 1.71 ± 0.041
4.186ValIle: 4.186 ± 0.064
1.835ValLys: 1.835 ± 0.042
9.318ValLeu: 9.318 ± 0.124
1.633ValMet: 1.633 ± 0.038
2.096ValAsn: 2.096 ± 0.052
4.459ValPro: 4.459 ± 0.068
2.458ValGln: 2.458 ± 0.045
5.353ValArg: 5.353 ± 0.081
5.21ValSer: 5.21 ± 0.074
5.284ValThr: 5.284 ± 0.074
8.154ValVal: 8.154 ± 0.116
0.993ValTrp: 0.993 ± 0.032
1.499ValTyr: 1.499 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.473TrpAla: 1.473 ± 0.048
0.091TrpCys: 0.091 ± 0.01
0.815TrpAsp: 0.815 ± 0.031
0.663TrpGlu: 0.663 ± 0.027
0.532TrpPhe: 0.532 ± 0.026
0.981TrpGly: 0.981 ± 0.032
0.364TrpHis: 0.364 ± 0.021
0.698TrpIle: 0.698 ± 0.03
0.358TrpLys: 0.358 ± 0.021
1.82TrpLeu: 1.82 ± 0.049
0.322TrpMet: 0.322 ± 0.018
0.465TrpAsn: 0.465 ± 0.026
0.602TrpPro: 0.602 ± 0.029
0.593TrpGln: 0.593 ± 0.028
1.073TrpArg: 1.073 ± 0.04
0.816TrpSer: 0.816 ± 0.028
0.812TrpThr: 0.812 ± 0.027
1.107TrpVal: 1.107 ± 0.035
0.343TrpTrp: 0.343 ± 0.021
0.262TrpTyr: 0.262 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.247TyrAla: 2.247 ± 0.044
0.162TyrCys: 0.162 ± 0.013
1.279TyrAsp: 1.279 ± 0.04
1.128TyrGlu: 1.128 ± 0.04
0.787TyrPhe: 0.787 ± 0.03
1.885TyrGly: 1.885 ± 0.046
0.335TyrHis: 0.335 ± 0.02
0.697TyrIle: 0.697 ± 0.025
0.387TyrLys: 0.387 ± 0.022
2.322TyrLeu: 2.322 ± 0.048
0.303TyrMet: 0.303 ± 0.021
0.495TyrAsn: 0.495 ± 0.024
1.097TyrPro: 1.097 ± 0.036
0.741TyrGln: 0.741 ± 0.028
1.615TyrArg: 1.615 ± 0.047
1.345TyrSer: 1.345 ± 0.042
1.158TyrThr: 1.158 ± 0.037
1.597TyrVal: 1.597 ± 0.044
0.325TyrTrp: 0.325 ± 0.018
0.483TyrTyr: 0.483 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2959 proteins (946945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski