Eucalyptus grandis (Flooded gum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A058ZYD9|A0A058ZYD9_EUCGR LRRNT_2 domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K00637 PE=4 SV=1
MM1 pKa = 7.4LGVVDD6 pKa = 5.4KK7 pKa = 11.08FPEE10 pKa = 4.69CSHH13 pKa = 6.38GFGFVTFDD21 pKa = 3.35EE22 pKa = 4.49KK23 pKa = 11.14QAMDD27 pKa = 3.8EE28 pKa = 4.91AIEE31 pKa = 4.26AMNGMDD37 pKa = 4.42LDD39 pKa = 3.59VRR41 pKa = 11.84NIIEE45 pKa = 4.55EE46 pKa = 4.16KK47 pKa = 10.62AQPQCSGGDD56 pKa = 3.31QDD58 pKa = 4.83VDD60 pKa = 4.23CSCKK64 pKa = 10.38CGPDD68 pKa = 3.73GDD70 pKa = 4.94SGTQGGSGNDD80 pKa = 3.55CC81 pKa = 4.07

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059C8X4|A0A059C8X4_EUCGR Uncharacterized protein (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E036382 PE=4 SV=1
MM1 pKa = 5.65VTRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AMVISRR21 pKa = 11.84SGKK24 pKa = 8.05MNGGGVSRR32 pKa = 11.84RR33 pKa = 11.84FSSRR37 pKa = 11.84PIPKK41 pKa = 9.67RR42 pKa = 11.84GQVKK46 pKa = 9.61VGIVVGLAHH55 pKa = 6.48SFVSIFSLHH64 pKa = 6.2LRR66 pKa = 11.84NISAQFSRR74 pKa = 4.36

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36664

7486

44150

16844312

8

4828

381.5

42.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.091 ± 0.012

1.936 ± 0.006

5.24 ± 0.008

6.321 ± 0.013

4.184 ± 0.008

6.881 ± 0.012

2.388 ± 0.006

5.229 ± 0.009

5.678 ± 0.01

10.242 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.005

4.145 ± 0.009

4.997 ± 0.011

3.476 ± 0.007

5.635 ± 0.009

8.81 ± 0.012

4.64 ± 0.007

6.545 ± 0.008

1.346 ± 0.004

2.768 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski