Tortoise microvirus 78

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W7R4|A0A4P8W7R4_9VIRU Uncharacterized protein OS=Tortoise microvirus 78 OX=2583185 PE=4 SV=1
MM1 pKa = 7.64AIKK4 pKa = 10.69VGDD7 pKa = 3.83LNRR10 pKa = 11.84WKK12 pKa = 10.23QLKK15 pKa = 10.61LGDD18 pKa = 4.06ALALNSDD25 pKa = 3.65RR26 pKa = 11.84QRR28 pKa = 11.84QVRR31 pKa = 11.84LEE33 pKa = 4.26VNVDD37 pKa = 3.14QPTQVMAVRR46 pKa = 11.84GTDD49 pKa = 3.39VVLLGVVMGMDD60 pKa = 3.85VIEE63 pKa = 4.67FVTDD67 pKa = 4.01GPTEE71 pKa = 3.98VAFTTEE77 pKa = 3.84GQVFYY82 pKa = 8.05FTRR85 pKa = 11.84DD86 pKa = 3.17GDD88 pKa = 4.31SEE90 pKa = 4.75ALDD93 pKa = 3.61LVEE96 pKa = 4.48EE97 pKa = 4.49VSFATLANRR106 pKa = 11.84RR107 pKa = 11.84ARR109 pKa = 11.84NPQVEE114 pKa = 4.32LMFYY118 pKa = 11.13KK119 pKa = 10.24MNQNMEE125 pKa = 4.02RR126 pKa = 11.84RR127 pKa = 11.84LAQQADD133 pKa = 3.61DD134 pKa = 3.88FEE136 pKa = 6.1RR137 pKa = 11.84RR138 pKa = 11.84MAEE141 pKa = 3.67MGYY144 pKa = 10.61DD145 pKa = 3.72PDD147 pKa = 4.0TGEE150 pKa = 4.99VADD153 pKa = 5.5DD154 pKa = 4.79DD155 pKa = 5.74DD156 pKa = 6.45DD157 pKa = 4.26GTVEE161 pKa = 4.42GDD163 pKa = 3.23TAAAEE168 pKa = 4.04AAAGGISGTDD178 pKa = 3.24AEE180 pKa = 4.76ASGDD184 pKa = 3.58AARR187 pKa = 11.84APGGGAGTAA196 pKa = 3.64

Molecular weight: 21.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FW10|A0A4V1FW10_9VIRU Uncharacterized protein OS=Tortoise microvirus 78 OX=2583185 PE=4 SV=1
MM1 pKa = 7.8PSHH4 pKa = 6.94SSSEE8 pKa = 4.21SLSGSEE14 pKa = 4.74GGEE17 pKa = 4.01TTLGRR22 pKa = 11.84TARR25 pKa = 11.84EE26 pKa = 3.43RR27 pKa = 11.84GARR30 pKa = 11.84LASQAPVQPPRR41 pKa = 11.84APDD44 pKa = 3.71PVILAPTNPITPVNPAQEE62 pKa = 3.69AAARR66 pKa = 11.84RR67 pKa = 11.84VHH69 pKa = 6.16SLPGGTLRR77 pKa = 11.84GTQNNTTRR85 pKa = 11.84TVSTAKK91 pKa = 9.93VRR93 pKa = 11.84PDD95 pKa = 3.22DD96 pKa = 3.98AKK98 pKa = 11.3ARR100 pKa = 11.84TQVQPSKK107 pKa = 10.76AARR110 pKa = 11.84DD111 pKa = 3.48DD112 pKa = 3.88RR113 pKa = 11.84EE114 pKa = 3.69PDD116 pKa = 3.4KK117 pKa = 11.26RR118 pKa = 11.84WVCKK122 pKa = 10.29ARR124 pKa = 11.84PEE126 pKa = 4.32SNKK129 pKa = 10.04PKK131 pKa = 10.61GGGGGAKK138 pKa = 9.72RR139 pKa = 11.84AFIPWCSS146 pKa = 3.07

Molecular weight: 15.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1800

69

541

225.0

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.778 ± 0.846

0.111 ± 0.114

5.722 ± 0.642

6.056 ± 0.858

3.111 ± 0.459

10.389 ± 0.913

1.833 ± 0.51

3.167 ± 0.49

4.5 ± 0.408

7.556 ± 0.92

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.436

4.056 ± 0.828

6.944 ± 0.971

4.778 ± 0.245

6.5 ± 0.717

4.944 ± 0.718

4.944 ± 0.682

7.333 ± 1.265

2.5 ± 0.593

2.444 ± 0.723
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski