Amino acid dipepetide frequency for Rhodobacteraceae bacterium AsT-22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.904AlaAla: 15.904 ± 0.17	1.074AlaCys: 1.074 ± 0.031	6.742AlaAsp: 6.742 ± 0.077	8.125AlaGlu: 8.125 ± 0.108	4.147AlaPhe: 4.147 ± 0.061	10.629AlaGly: 10.629 ± 0.099	2.579AlaHis: 2.579 ± 0.05	5.951AlaIle: 5.951 ± 0.072	3.438AlaLys: 3.438 ± 0.065	13.729AlaLeu: 13.729 ± 0.134	3.927AlaMet: 3.927 ± 0.058	2.636AlaAsn: 2.636 ± 0.042	5.848AlaPro: 5.848 ± 0.074	4.264AlaGln: 4.264 ± 0.065	10.081AlaArg: 10.081 ± 0.114	5.294AlaSer: 5.294 ± 0.071	5.706AlaThr: 5.706 ± 0.07	8.337AlaVal: 8.337 ± 0.098	1.473AlaTrp: 1.473 ± 0.037	2.443AlaTyr: 2.443 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.06CysAla: 1.06 ± 0.033	0.108CysCys: 0.108 ± 0.01	0.657CysAsp: 0.657 ± 0.023	0.458CysGlu: 0.458 ± 0.017	0.303CysPhe: 0.303 ± 0.016	0.957CysGly: 0.957 ± 0.034	0.317CysHis: 0.317 ± 0.016	0.423CysIle: 0.423 ± 0.019	0.202CysLys: 0.202 ± 0.012	0.836CysLeu: 0.836 ± 0.025	0.158CysMet: 0.158 ± 0.01	0.216CysAsn: 0.216 ± 0.013	0.566CysPro: 0.566 ± 0.021	0.21CysGln: 0.21 ± 0.014	0.549CysArg: 0.549 ± 0.021	0.437CysSer: 0.437 ± 0.02	0.466CysThr: 0.466 ± 0.02	0.632CysVal: 0.632 ± 0.026	0.102CysTrp: 0.102 ± 0.01	0.219CysTyr: 0.219 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.199AspAla: 7.199 ± 0.078	0.552AspCys: 0.552 ± 0.019	3.589AspAsp: 3.589 ± 0.071	3.796AspGlu: 3.796 ± 0.064	2.411AspPhe: 2.411 ± 0.046	5.436AspGly: 5.436 ± 0.073	1.521AspHis: 1.521 ± 0.043	3.19AspIle: 3.19 ± 0.046	1.613AspLys: 1.613 ± 0.033	6.29AspLeu: 6.29 ± 0.083	1.798AspMet: 1.798 ± 0.04	1.256AspAsn: 1.256 ± 0.033	3.868AspPro: 3.868 ± 0.065	1.679AspGln: 1.679 ± 0.039	4.521AspArg: 4.521 ± 0.065	2.097AspSer: 2.097 ± 0.037	3.022AspThr: 3.022 ± 0.049	4.318AspVal: 4.318 ± 0.068	1.191AspTrp: 1.191 ± 0.032	1.586AspTyr: 1.586 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	8.139GluAla: 8.139 ± 0.103	0.412GluCys: 0.412 ± 0.018	3.431GluAsp: 3.431 ± 0.056	3.743GluGlu: 3.743 ± 0.076	1.871GluPhe: 1.871 ± 0.04	5.024GluGly: 5.024 ± 0.069	1.163GluHis: 1.163 ± 0.036	3.931GluIle: 3.931 ± 0.055	2.273GluLys: 2.273 ± 0.055	5.055GluLeu: 5.055 ± 0.067	2.08GluMet: 2.08 ± 0.042	1.821GluAsn: 1.821 ± 0.036	2.664GluPro: 2.664 ± 0.038	1.89GluGln: 1.89 ± 0.037	4.658GluArg: 4.658 ± 0.065	2.488GluSer: 2.488 ± 0.047	4.151GluThr: 4.151 ± 0.063	4.378GluVal: 4.378 ± 0.061	0.764GluTrp: 0.764 ± 0.026	1.067GluTyr: 1.067 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.449PheAla: 4.449 ± 0.067	0.423PheCys: 0.423 ± 0.02	2.829PheAsp: 2.829 ± 0.047	2.313PheGlu: 2.313 ± 0.043	1.468PhePhe: 1.468 ± 0.037	3.71PheGly: 3.71 ± 0.063	0.821PheHis: 0.821 ± 0.027	1.678PheIle: 1.678 ± 0.038	0.838PheLys: 0.838 ± 0.026	3.477PheLeu: 3.477 ± 0.051	0.892PheMet: 0.892 ± 0.026	1.043PheAsn: 1.043 ± 0.028	1.633PhePro: 1.633 ± 0.037	0.987PheGln: 0.987 ± 0.026	2.326PheArg: 2.326 ± 0.048	2.084PheSer: 2.084 ± 0.039	2.034PheThr: 2.034 ± 0.04	2.593PheVal: 2.593 ± 0.056	0.607PheTrp: 0.607 ± 0.021	0.913PheTyr: 0.913 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.16GlyAla: 10.16 ± 0.104	0.882GlyCys: 0.882 ± 0.032	4.983GlyAsp: 4.983 ± 0.072	5.166GlyGlu: 5.166 ± 0.072	3.853GlyPhe: 3.853 ± 0.06	7.683GlyGly: 7.683 ± 0.114	2.14GlyHis: 2.14 ± 0.049	4.48GlyIle: 4.48 ± 0.064	2.989GlyLys: 2.989 ± 0.054	9.512GlyLeu: 9.512 ± 0.105	2.858GlyMet: 2.858 ± 0.055	2.034GlyAsn: 2.034 ± 0.042	3.887GlyPro: 3.887 ± 0.057	3.149GlyGln: 3.149 ± 0.048	6.272GlyArg: 6.272 ± 0.079	4.047GlySer: 4.047 ± 0.085	4.526GlyThr: 4.526 ± 0.064	6.589GlyVal: 6.589 ± 0.08	1.541GlyTrp: 1.541 ± 0.037	2.286GlyTyr: 2.286 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.522HisAla: 2.522 ± 0.049	0.264HisCys: 0.264 ± 0.015	1.469HisAsp: 1.469 ± 0.034	1.161HisGlu: 1.161 ± 0.031	0.868HisPhe: 0.868 ± 0.028	2.156HisGly: 2.156 ± 0.046	0.583HisHis: 0.583 ± 0.023	1.005HisIle: 1.005 ± 0.029	0.502HisLys: 0.502 ± 0.02	2.238HisLeu: 2.238 ± 0.042	0.557HisMet: 0.557 ± 0.02	0.457HisAsn: 0.457 ± 0.019	1.478HisPro: 1.478 ± 0.033	0.561HisGln: 0.561 ± 0.022	1.399HisArg: 1.399 ± 0.028	0.952HisSer: 0.952 ± 0.03	0.855HisThr: 0.855 ± 0.03	1.737HisVal: 1.737 ± 0.035	0.382HisTrp: 0.382 ± 0.019	0.576HisTyr: 0.576 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.125IleAla: 7.125 ± 0.079	0.617IleCys: 0.617 ± 0.024	3.485IleAsp: 3.485 ± 0.05	3.853IleGlu: 3.853 ± 0.057	1.751IlePhe: 1.751 ± 0.046	4.824IleGly: 4.824 ± 0.069	1.001IleHis: 1.001 ± 0.028	2.256IleIle: 2.256 ± 0.051	1.355IleLys: 1.355 ± 0.033	4.905IleLeu: 4.905 ± 0.079	1.162IleMet: 1.162 ± 0.031	1.303IleAsn: 1.303 ± 0.033	2.418IlePro: 2.418 ± 0.05	1.131IleGln: 1.131 ± 0.027	3.418IleArg: 3.418 ± 0.051	2.843IleSer: 2.843 ± 0.046	2.929IleThr: 2.929 ± 0.052	3.909IleVal: 3.909 ± 0.064	0.727IleTrp: 0.727 ± 0.028	1.111IleTyr: 1.111 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.615LysAla: 3.615 ± 0.061	0.182LysCys: 0.182 ± 0.011	1.555LysAsp: 1.555 ± 0.038	1.507LysGlu: 1.507 ± 0.037	0.808LysPhe: 0.808 ± 0.025	2.629LysGly: 2.629 ± 0.041	0.579LysHis: 0.579 ± 0.023	1.518LysIle: 1.518 ± 0.034	1.008LysLys: 1.008 ± 0.03	2.792LysLeu: 2.792 ± 0.049	0.815LysMet: 0.815 ± 0.029	0.705LysAsn: 0.705 ± 0.023	1.808LysPro: 1.808 ± 0.04	0.844LysGln: 0.844 ± 0.031	2.257LysArg: 2.257 ± 0.043	1.7LysSer: 1.7 ± 0.038	1.869LysThr: 1.869 ± 0.038	2.087LysVal: 2.087 ± 0.045	0.397LysTrp: 0.397 ± 0.02	0.611LysTyr: 0.611 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.22LeuAla: 13.22 ± 0.14	0.933LeuCys: 0.933 ± 0.025	6.392LeuAsp: 6.392 ± 0.087	5.394LeuGlu: 5.394 ± 0.068	3.567LeuPhe: 3.567 ± 0.067	8.735LeuGly: 8.735 ± 0.106	1.93LeuHis: 1.93 ± 0.04	5.27LeuIle: 5.27 ± 0.085	2.969LeuLys: 2.969 ± 0.058	8.612LeuLeu: 8.612 ± 0.115	2.741LeuMet: 2.741 ± 0.053	2.609LeuAsn: 2.609 ± 0.05	5.486LeuPro: 5.486 ± 0.075	2.459LeuGln: 2.459 ± 0.047	7.278LeuArg: 7.278 ± 0.091	6.398LeuSer: 6.398 ± 0.089	5.649LeuThr: 5.649 ± 0.07	6.874LeuVal: 6.874 ± 0.092	1.315LeuTrp: 1.315 ± 0.033	1.937LeuTyr: 1.937 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	3.631MetAla: 3.631 ± 0.052	0.189MetCys: 0.189 ± 0.014	1.374MetAsp: 1.374 ± 0.032	1.437MetGlu: 1.437 ± 0.036	0.838MetPhe: 0.838 ± 0.027	2.487MetGly: 2.487 ± 0.051	0.506MetHis: 0.506 ± 0.021	1.715MetIle: 1.715 ± 0.039	1.101MetLys: 1.101 ± 0.034	2.775MetLeu: 2.775 ± 0.045	0.824MetMet: 0.824 ± 0.03	0.851MetAsn: 0.851 ± 0.027	1.551MetPro: 1.551 ± 0.036	1.048MetGln: 1.048 ± 0.028	2.152MetArg: 2.152 ± 0.043	1.811MetSer: 1.811 ± 0.038	2.069MetThr: 2.069 ± 0.044	1.978MetVal: 1.978 ± 0.036	0.259MetTrp: 0.259 ± 0.016	0.344MetTyr: 0.344 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.094AsnAla: 3.094 ± 0.056	0.253AsnCys: 0.253 ± 0.014	1.429AsnAsp: 1.429 ± 0.033	1.198AsnGlu: 1.198 ± 0.029	0.935AsnPhe: 0.935 ± 0.03	2.235AsnGly: 2.235 ± 0.051	0.508AsnHis: 0.508 ± 0.016	1.333AsnIle: 1.333 ± 0.032	0.609AsnLys: 0.609 ± 0.023	2.491AsnLeu: 2.491 ± 0.045	0.688AsnMet: 0.688 ± 0.022	0.606AsnAsn: 0.606 ± 0.024	1.916AsnPro: 1.916 ± 0.035	0.636AsnGln: 0.636 ± 0.021	1.785AsnArg: 1.785 ± 0.034	0.994AsnSer: 0.994 ± 0.03	1.28AsnThr: 1.28 ± 0.028	1.758AsnVal: 1.758 ± 0.038	0.439AsnTrp: 0.439 ± 0.018	0.592AsnTyr: 0.592 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.517ProAla: 5.517 ± 0.064	0.382ProCys: 0.382 ± 0.017	4.198ProAsp: 4.198 ± 0.074	4.442ProGlu: 4.442 ± 0.057	1.964ProPhe: 1.964 ± 0.038	5.115ProGly: 5.115 ± 0.062	1.177ProHis: 1.177 ± 0.03	2.256ProIle: 2.256 ± 0.046	1.509ProLys: 1.509 ± 0.038	4.648ProLeu: 4.648 ± 0.065	1.384ProMet: 1.384 ± 0.035	1.273ProAsn: 1.273 ± 0.029	2.48ProPro: 2.48 ± 0.052	1.592ProGln: 1.592 ± 0.037	3.107ProArg: 3.107 ± 0.053	2.346ProSer: 2.346 ± 0.046	2.357ProThr: 2.357 ± 0.042	4.438ProVal: 4.438 ± 0.061	0.681ProTrp: 0.681 ± 0.024	1.122ProTyr: 1.122 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.808GlnAla: 3.808 ± 0.06	0.192GlnCys: 0.192 ± 0.012	1.582GlnAsp: 1.582 ± 0.037	1.569GlnGlu: 1.569 ± 0.035	1.055GlnPhe: 1.055 ± 0.028	2.601GlnGly: 2.601 ± 0.044	0.568GlnHis: 0.568 ± 0.017	1.832GlnIle: 1.832 ± 0.037	1.004GlnLys: 1.004 ± 0.031	2.553GlnLeu: 2.553 ± 0.046	0.981GlnMet: 0.981 ± 0.028	0.845GlnAsn: 0.845 ± 0.026	1.536GlnPro: 1.536 ± 0.037	0.94GlnGln: 0.94 ± 0.03	2.134GlnArg: 2.134 ± 0.038	1.745GlnSer: 1.745 ± 0.037	1.682GlnThr: 1.682 ± 0.037	2.285GlnVal: 2.285 ± 0.039	0.382GlnTrp: 0.382 ± 0.016	0.571GlnTyr: 0.571 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.075ArgAla: 9.075 ± 0.105	0.506ArgCys: 0.506 ± 0.021	4.685ArgAsp: 4.685 ± 0.058	4.187ArgGlu: 4.187 ± 0.067	2.956ArgPhe: 2.956 ± 0.053	5.044ArgGly: 5.044 ± 0.066	1.728ArgHis: 1.728 ± 0.042	4.117ArgIle: 4.117 ± 0.056	2.228ArgLys: 2.228 ± 0.046	7.808ArgLeu: 7.808 ± 0.1	2.208ArgMet: 2.208 ± 0.04	1.837ArgAsn: 1.837 ± 0.039	3.557ArgPro: 3.557 ± 0.06	2.262ArgGln: 2.262 ± 0.042	5.361ArgArg: 5.361 ± 0.075	3.253ArgSer: 3.253 ± 0.055	3.01ArgThr: 3.01 ± 0.054	5.175ArgVal: 5.175 ± 0.069	0.957ArgTrp: 0.957 ± 0.031	1.554ArgTyr: 1.554 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.395SerAla: 5.395 ± 0.062	0.418SerCys: 0.418 ± 0.02	3.227SerAsp: 3.227 ± 0.05	2.969SerGlu: 2.969 ± 0.052	2.157SerPhe: 2.157 ± 0.04	5.355SerGly: 5.355 ± 0.071	1.111SerHis: 1.111 ± 0.034	2.355SerIle: 2.355 ± 0.049	1.365SerLys: 1.365 ± 0.032	4.757SerLeu: 4.757 ± 0.065	1.31SerMet: 1.31 ± 0.034	1.259SerAsn: 1.259 ± 0.036	2.477SerPro: 2.477 ± 0.044	1.422SerGln: 1.422 ± 0.036	3.282SerArg: 3.282 ± 0.053	2.296SerSer: 2.296 ± 0.052	2.415SerThr: 2.415 ± 0.045	3.703SerVal: 3.703 ± 0.078	0.667SerTrp: 0.667 ± 0.023	1.256SerTyr: 1.256 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	5.841ThrAla: 5.841 ± 0.063	0.504ThrCys: 0.504 ± 0.02	3.025ThrAsp: 3.025 ± 0.044	2.925ThrGlu: 2.925 ± 0.047	1.817ThrPhe: 1.817 ± 0.041	5.61ThrGly: 5.61 ± 0.073	1.164ThrHis: 1.164 ± 0.032	2.729ThrIle: 2.729 ± 0.046	1.2ThrLys: 1.2 ± 0.028	5.888ThrLeu: 5.888 ± 0.072	1.341ThrMet: 1.341 ± 0.036	1.202ThrAsn: 1.202 ± 0.03	3.388ThrPro: 3.388 ± 0.056	1.484ThrGln: 1.484 ± 0.033	3.884ThrArg: 3.884 ± 0.061	2.487ThrSer: 2.487 ± 0.051	2.734ThrThr: 2.734 ± 0.051	3.92ThrVal: 3.92 ± 0.06	0.7ThrTrp: 0.7 ± 0.025	1.198ThrTyr: 1.198 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	8.72ValAla: 8.72 ± 0.107	0.598ValCys: 0.598 ± 0.023	4.013ValAsp: 4.013 ± 0.057	4.731ValGlu: 4.731 ± 0.069	2.87ValPhe: 2.87 ± 0.049	5.61ValGly: 5.61 ± 0.101	1.422ValHis: 1.422 ± 0.034	4.472ValIle: 4.472 ± 0.073	2.111ValLys: 2.111 ± 0.051	7.498ValLeu: 7.498 ± 0.09	2.294ValMet: 2.294 ± 0.044	1.881ValAsn: 1.881 ± 0.039	3.622ValPro: 3.622 ± 0.057	2.071ValGln: 2.071 ± 0.042	4.305ValArg: 4.305 ± 0.061	4.101ValSer: 4.101 ± 0.061	4.534ValThr: 4.534 ± 0.064	5.622ValVal: 5.622 ± 0.075	0.899ValTrp: 0.899 ± 0.028	1.423ValTyr: 1.423 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.418TrpAla: 1.418 ± 0.035	0.145TrpCys: 0.145 ± 0.012	0.748TrpAsp: 0.748 ± 0.025	0.707TrpGlu: 0.707 ± 0.029	0.572TrpPhe: 0.572 ± 0.021	1.069TrpGly: 1.069 ± 0.032	0.349TrpHis: 0.349 ± 0.017	0.711TrpIle: 0.711 ± 0.023	0.441TrpLys: 0.441 ± 0.021	1.67TrpLeu: 1.67 ± 0.039	0.444TrpMet: 0.444 ± 0.018	0.428TrpAsn: 0.428 ± 0.02	0.719TrpPro: 0.719 ± 0.027	0.594TrpGln: 0.594 ± 0.021	1.154TrpArg: 1.154 ± 0.03	0.763TrpSer: 0.763 ± 0.023	0.693TrpThr: 0.693 ± 0.024	0.953TrpVal: 0.953 ± 0.022	0.234TrpTrp: 0.234 ± 0.012	0.263TrpTyr: 0.263 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.436TyrAla: 2.436 ± 0.044	0.241TyrCys: 0.241 ± 0.013	1.518TyrAsp: 1.518 ± 0.035	1.321TyrGlu: 1.321 ± 0.036	0.909TyrPhe: 0.909 ± 0.029	2.129TyrGly: 2.129 ± 0.047	0.553TyrHis: 0.553 ± 0.023	0.902TyrIle: 0.902 ± 0.028	0.529TyrLys: 0.529 ± 0.019	2.235TyrLeu: 2.235 ± 0.037	0.478TyrMet: 0.478 ± 0.018	0.516TyrAsn: 0.516 ± 0.019	1.084TyrPro: 1.084 ± 0.026	0.645TyrGln: 0.645 ± 0.024	1.585TyrArg: 1.585 ± 0.038	1.062TyrSer: 1.062 ± 0.03	1.059TyrThr: 1.059 ± 0.031	1.512TyrVal: 1.512 ± 0.028	0.358TyrTrp: 0.358 ± 0.017	0.543TyrTyr: 0.543 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4150 proteins (1294212 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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