Gonium pectorale (Green alga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Chlamydomonadales; Volvocaceae; Gonium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150H2N5|A0A150H2N5_GONPE Prolyl aminopeptidase OS=Gonium pectorale OX=33097 GN=GPECTOR_2g980 PE=4 SV=1
MM1 pKa = 6.94NTIEE5 pKa = 5.37DD6 pKa = 4.12DD7 pKa = 4.23CAHH10 pKa = 6.74HH11 pKa = 6.95ALLPEE16 pKa = 4.33GHH18 pKa = 5.91STLYY22 pKa = 10.74DD23 pKa = 3.69ALPSFDD29 pKa = 4.61EE30 pKa = 4.9DD31 pKa = 3.48LSEE34 pKa = 4.74DD35 pKa = 3.92VIVPLSTPPEE45 pKa = 4.16TEE47 pKa = 4.11PVDD50 pKa = 3.89EE51 pKa = 4.39THH53 pKa = 6.87GGASSSLPATPAEE66 pKa = 4.54RR67 pKa = 11.84PCTFCPASLPLPPASGPSRR86 pKa = 11.84VTLVLDD92 pKa = 4.75LDD94 pKa = 3.93GTLIASEE101 pKa = 4.46EE102 pKa = 3.97LNAANSAFLWNPHH115 pKa = 6.02CGARR119 pKa = 11.84DD120 pKa = 3.56PDD122 pKa = 3.94YY123 pKa = 10.69EE124 pKa = 4.68AMGRR128 pKa = 11.84RR129 pKa = 11.84VWLRR133 pKa = 11.84PGVRR137 pKa = 11.84DD138 pKa = 3.57FLAAVRR144 pKa = 11.84PHH146 pKa = 6.39FEE148 pKa = 3.74IVLFTAATQNWAAAAIEE165 pKa = 3.91QLDD168 pKa = 3.7PSAYY172 pKa = 10.42LFDD175 pKa = 4.07VMLHH179 pKa = 6.58RR180 pKa = 11.84DD181 pKa = 3.46HH182 pKa = 6.48TTSDD186 pKa = 3.71LMWDD190 pKa = 3.71YY191 pKa = 11.88VKK193 pKa = 10.88DD194 pKa = 3.95LSRR197 pKa = 11.84LGRR200 pKa = 11.84DD201 pKa = 3.24LARR204 pKa = 11.84VVIVDD209 pKa = 4.46DD210 pKa = 4.08NPLMFMYY217 pKa = 10.53QPDD220 pKa = 3.79NALHH224 pKa = 6.07IGAYY228 pKa = 8.4EE229 pKa = 4.0AASAGGPDD237 pKa = 3.41NVLEE241 pKa = 4.27RR242 pKa = 11.84VAEE245 pKa = 4.07LLVSKK250 pKa = 10.31VAVAEE255 pKa = 4.15DD256 pKa = 3.48VRR258 pKa = 11.84EE259 pKa = 4.04VLGPLARR266 pKa = 11.84AKK268 pKa = 10.6SCITSTVNAKK278 pKa = 9.7AAPGTAAEE286 pKa = 4.55PLAATASCPAADD298 pKa = 4.29GADD301 pKa = 3.5SAVAAGAAPGDD312 pKa = 3.96GSASTEE318 pKa = 4.0AEE320 pKa = 3.79FDD322 pKa = 3.69GSGAAADD329 pKa = 4.29TDD331 pKa = 4.35TSCDD335 pKa = 3.31AADD338 pKa = 4.15ASNSGAEE345 pKa = 4.21CHH347 pKa = 5.88GAAPEE352 pKa = 4.14GVEE355 pKa = 4.43GEE357 pKa = 4.62EE358 pKa = 4.44EE359 pKa = 4.29VQQDD363 pKa = 4.03EE364 pKa = 4.58QEE366 pKa = 4.31HH367 pKa = 6.14CAGAGDD373 pKa = 4.28EE374 pKa = 4.2EE375 pKa = 5.01LEE377 pKa = 4.46AVDD380 pKa = 5.35SEE382 pKa = 4.58DD383 pKa = 3.55AAHH386 pKa = 6.17VKK388 pKa = 10.25HH389 pKa = 6.93DD390 pKa = 4.3EE391 pKa = 4.15EE392 pKa = 4.33EE393 pKa = 4.22ALPGGDD399 pKa = 3.92LRR401 pKa = 11.84CTEE404 pKa = 4.21GADD407 pKa = 5.0AAFDD411 pKa = 3.64WAGEE415 pKa = 4.09SDD417 pKa = 3.78VPEE420 pKa = 4.58IFDD423 pKa = 3.39EE424 pKa = 4.83HH425 pKa = 6.48GAAACLGADD434 pKa = 4.82SSWQQQSCGGYY445 pKa = 10.56AVDD448 pKa = 5.15APAGEE453 pKa = 4.87DD454 pKa = 3.73SQDD457 pKa = 3.44SAAEE461 pKa = 3.99EE462 pKa = 4.09QPQQHH467 pKa = 6.29NSTTEE472 pKa = 3.81SDD474 pKa = 3.29GHH476 pKa = 7.47DD477 pKa = 4.18DD478 pKa = 5.15FDD480 pKa = 6.55ALMDD484 pKa = 4.44DD485 pKa = 5.75AIAEE489 pKa = 4.31YY490 pKa = 10.77DD491 pKa = 3.21SDD493 pKa = 5.35AYY495 pKa = 10.72DD496 pKa = 3.32GQYY499 pKa = 11.3DD500 pKa = 3.44VDD502 pKa = 3.5IPYY505 pKa = 10.45DD506 pKa = 3.76AEE508 pKa = 4.53CPAGTAAAVASDD520 pKa = 3.46GGAANRR526 pKa = 11.84ASDD529 pKa = 4.61DD530 pKa = 3.86DD531 pKa = 3.9VAEE534 pKa = 5.26AGLQDD539 pKa = 4.9PLRR542 pKa = 11.84QPPVGMVLLADD553 pKa = 4.04LHH555 pKa = 6.62PCLGSNTSGVSPTALLGSKK574 pKa = 9.96RR575 pKa = 11.84ARR577 pKa = 11.84DD578 pKa = 3.47EE579 pKa = 4.81DD580 pKa = 4.28GEE582 pKa = 4.42EE583 pKa = 4.27ACNAAAPHH591 pKa = 6.12CRR593 pKa = 11.84MRR595 pKa = 11.84PAGFACSALEE605 pKa = 4.04TVQVGSCEE613 pKa = 3.93LL614 pKa = 3.47

Molecular weight: 63.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150GNU4|A0A150GNU4_GONPE Uncharacterized protein OS=Gonium pectorale OX=33097 GN=GPECTOR_12g418 PE=4 SV=1
MM1 pKa = 7.58AATASMVALVTSMASVAALMAVAALVTAALVTAALVMAALVTAALVTVALVSAALVAAALVTAALMTAALVTAALVTAALMTAALVTAALVTAALVTAALVTAALVTAALVSAALVTAALVTAALGTAALVMAALVMAALVTAALVTAALVTPALVKK158 pKa = 10.49AALVTPAALVTVVVAPVVAAAASVAALVVARR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84WRR193 pKa = 11.84RR194 pKa = 11.84WWRR197 pKa = 11.84RR198 pKa = 11.84PRR200 pKa = 11.84WWPRR204 pKa = 11.84WWPRR208 pKa = 11.84RR209 pKa = 11.84WRR211 pKa = 3.63

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16224

0

16224

8125833

54

8881

500.9

52.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.459 ± 0.051

1.462 ± 0.01

4.791 ± 0.013

5.552 ± 0.025

2.402 ± 0.013

10.339 ± 0.029

2.039 ± 0.008

2.369 ± 0.014

2.783 ± 0.019

9.655 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.979 ± 0.008

2.115 ± 0.014

6.855 ± 0.024

3.94 ± 0.019

6.696 ± 0.019

7.064 ± 0.018

4.615 ± 0.015

6.586 ± 0.018

1.302 ± 0.007

1.996 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski