Amino acid dipepetide frequency for Paenibacillus sp. UNC496MF

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.241AlaAla: 14.241 ± 0.174
0.932AlaCys: 0.932 ± 0.019
6.273AlaAsp: 6.273 ± 0.073
7.061AlaGlu: 7.061 ± 0.096
4.426AlaPhe: 4.426 ± 0.048
9.529AlaGly: 9.529 ± 0.092
1.773AlaHis: 1.773 ± 0.033
5.539AlaIle: 5.539 ± 0.059
4.881AlaLys: 4.881 ± 0.058
10.34AlaLeu: 10.34 ± 0.086
2.829AlaMet: 2.829 ± 0.039
3.201AlaAsn: 3.201 ± 0.043
3.999AlaPro: 3.999 ± 0.056
3.04AlaGln: 3.04 ± 0.043
5.532AlaArg: 5.532 ± 0.067
6.189AlaSer: 6.189 ± 0.055
4.031AlaThr: 4.031 ± 0.059
8.103AlaVal: 8.103 ± 0.069
1.382AlaTrp: 1.382 ± 0.03
3.396AlaTyr: 3.396 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.723CysAla: 0.723 ± 0.02
0.103CysCys: 0.103 ± 0.007
0.363CysAsp: 0.363 ± 0.014
0.417CysGlu: 0.417 ± 0.015
0.321CysPhe: 0.321 ± 0.012
0.853CysGly: 0.853 ± 0.022
0.183CysHis: 0.183 ± 0.01
0.373CysIle: 0.373 ± 0.012
0.258CysLys: 0.258 ± 0.012
0.745CysLeu: 0.745 ± 0.022
0.184CysMet: 0.184 ± 0.008
0.211CysAsn: 0.211 ± 0.01
0.326CysPro: 0.326 ± 0.015
0.18CysGln: 0.18 ± 0.009
0.492CysArg: 0.492 ± 0.015
0.488CysSer: 0.488 ± 0.017
0.345CysThr: 0.345 ± 0.014
0.459CysVal: 0.459 ± 0.016
0.099CysTrp: 0.099 ± 0.006
0.225CysTyr: 0.225 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
6.212AspAla: 6.212 ± 0.071
0.375AspCys: 0.375 ± 0.016
2.835AspAsp: 2.835 ± 0.042
3.777AspGlu: 3.777 ± 0.045
2.123AspPhe: 2.123 ± 0.034
5.325AspGly: 5.325 ± 0.068
1.026AspHis: 1.026 ± 0.02
3.055AspIle: 3.055 ± 0.043
2.437AspLys: 2.437 ± 0.043
4.723AspLeu: 4.723 ± 0.051
1.39AspMet: 1.39 ± 0.029
1.719AspAsn: 1.719 ± 0.029
2.783AspPro: 2.783 ± 0.039
1.526AspGln: 1.526 ± 0.03
3.555AspArg: 3.555 ± 0.039
2.302AspSer: 2.302 ± 0.038
2.305AspThr: 2.305 ± 0.037
4.085AspVal: 4.085 ± 0.045
0.938AspTrp: 0.938 ± 0.022
2.179AspTyr: 2.179 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
7.512GluAla: 7.512 ± 0.089
0.308GluCys: 0.308 ± 0.012
2.941GluAsp: 2.941 ± 0.042
4.35GluGlu: 4.35 ± 0.057
1.821GluPhe: 1.821 ± 0.032
4.361GluGly: 4.361 ± 0.049
1.331GluHis: 1.331 ± 0.028
3.358GluIle: 3.358 ± 0.049
2.861GluLys: 2.861 ± 0.046
7.134GluLeu: 7.134 ± 0.068
1.612GluMet: 1.612 ± 0.027
1.799GluAsn: 1.799 ± 0.03
2.664GluPro: 2.664 ± 0.042
2.8GluGln: 2.8 ± 0.041
5.024GluArg: 5.024 ± 0.074
2.981GluSer: 2.981 ± 0.037
3.445GluThr: 3.445 ± 0.046
3.503GluVal: 3.503 ± 0.048
0.889GluTrp: 0.889 ± 0.021
1.544GluTyr: 1.544 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.216PheAla: 4.216 ± 0.053
0.335PheCys: 0.335 ± 0.015
2.435PheAsp: 2.435 ± 0.037
2.277PheGlu: 2.277 ± 0.034
1.744PhePhe: 1.744 ± 0.04
3.657PheGly: 3.657 ± 0.048
0.87PheHis: 0.87 ± 0.022
2.3PheIle: 2.3 ± 0.04
1.781PheLys: 1.781 ± 0.029
3.686PheLeu: 3.686 ± 0.054
1.064PheMet: 1.064 ± 0.02
1.391PheAsn: 1.391 ± 0.029
1.656PhePro: 1.656 ± 0.031
1.204PheGln: 1.204 ± 0.025
2.476PheArg: 2.476 ± 0.033
2.4PheSer: 2.4 ± 0.038
2.113PheThr: 2.113 ± 0.031
3.164PheVal: 3.164 ± 0.043
0.551PheTrp: 0.551 ± 0.017
1.395PheTyr: 1.395 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
7.981GlyAla: 7.981 ± 0.109
0.779GlyCys: 0.779 ± 0.019
4.251GlyAsp: 4.251 ± 0.052
4.994GlyGlu: 4.994 ± 0.058
3.517GlyPhe: 3.517 ± 0.044
7.418GlyGly: 7.418 ± 0.094
1.57GlyHis: 1.57 ± 0.029
5.3GlyIle: 5.3 ± 0.053
4.365GlyLys: 4.365 ± 0.059
7.981GlyLeu: 7.981 ± 0.075
2.467GlyMet: 2.467 ± 0.035
2.658GlyAsn: 2.658 ± 0.05
2.566GlyPro: 2.566 ± 0.052
2.662GlyGln: 2.662 ± 0.039
4.902GlyArg: 4.902 ± 0.07
5.048GlySer: 5.048 ± 0.067
4.803GlyThr: 4.803 ± 0.053
5.457GlyVal: 5.457 ± 0.049
1.262GlyTrp: 1.262 ± 0.025
3.047GlyTyr: 3.047 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.022HisAla: 2.022 ± 0.031
0.191HisCys: 0.191 ± 0.009
1.098HisAsp: 1.098 ± 0.022
1.178HisGlu: 1.178 ± 0.025
0.95HisPhe: 0.95 ± 0.022
1.788HisGly: 1.788 ± 0.032
0.513HisHis: 0.513 ± 0.018
1.039HisIle: 1.039 ± 0.024
0.63HisLys: 0.63 ± 0.016
1.884HisLeu: 1.884 ± 0.034
0.499HisMet: 0.499 ± 0.014
0.568HisAsn: 0.568 ± 0.019
1.283HisPro: 1.283 ± 0.028
0.613HisGln: 0.613 ± 0.019
1.279HisArg: 1.279 ± 0.026
0.89HisSer: 0.89 ± 0.025
0.875HisThr: 0.875 ± 0.022
1.47HisVal: 1.47 ± 0.027
0.319HisTrp: 0.319 ± 0.014
0.881HisTyr: 0.881 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.479IleAla: 6.479 ± 0.059
0.457IleCys: 0.457 ± 0.014
3.474IleAsp: 3.474 ± 0.044
3.453IleGlu: 3.453 ± 0.043
1.856IlePhe: 1.856 ± 0.039
5.28IleGly: 5.28 ± 0.055
1.148IleHis: 1.148 ± 0.024
2.827IleIle: 2.827 ± 0.053
2.128IleLys: 2.128 ± 0.037
4.543IleLeu: 4.543 ± 0.057
1.293IleMet: 1.293 ± 0.028
1.805IleAsn: 1.805 ± 0.036
2.709IlePro: 2.709 ± 0.037
1.795IleGln: 1.795 ± 0.031
3.694IleArg: 3.694 ± 0.048
3.024IleSer: 3.024 ± 0.047
2.742IleThr: 2.742 ± 0.045
4.934IleVal: 4.934 ± 0.051
0.603IleTrp: 0.603 ± 0.018
1.609IleTyr: 1.609 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.523LysAla: 4.523 ± 0.054
0.186LysCys: 0.186 ± 0.01
2.632LysAsp: 2.632 ± 0.045
3.128LysGlu: 3.128 ± 0.045
1.317LysPhe: 1.317 ± 0.025
3.116LysGly: 3.116 ± 0.045
0.996LysHis: 0.996 ± 0.024
2.262LysIle: 2.262 ± 0.037
2.325LysLys: 2.325 ± 0.045
5.009LysLeu: 5.009 ± 0.057
1.172LysMet: 1.172 ± 0.025
1.515LysAsn: 1.515 ± 0.035
2.462LysPro: 2.462 ± 0.034
2.006LysGln: 2.006 ± 0.029
3.138LysArg: 3.138 ± 0.043
2.378LysSer: 2.378 ± 0.037
2.544LysThr: 2.544 ± 0.04
2.717LysVal: 2.717 ± 0.044
0.655LysTrp: 0.655 ± 0.018
1.35LysTyr: 1.35 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
10.713LeuAla: 10.713 ± 0.097
0.8LeuCys: 0.8 ± 0.022
5.26LeuAsp: 5.26 ± 0.057
5.625LeuGlu: 5.625 ± 0.07
4.456LeuPhe: 4.456 ± 0.065
7.237LeuGly: 7.237 ± 0.064
2.053LeuHis: 2.053 ± 0.03
5.429LeuIle: 5.429 ± 0.062
4.558LeuLys: 4.558 ± 0.052
11.204LeuLeu: 11.204 ± 0.114
2.424LeuMet: 2.424 ± 0.036
3.395LeuAsn: 3.395 ± 0.047
5.0LeuPro: 5.0 ± 0.046
3.522LeuGln: 3.522 ± 0.038
6.34LeuArg: 6.34 ± 0.072
6.169LeuSer: 6.169 ± 0.07
5.673LeuThr: 5.673 ± 0.05
6.384LeuVal: 6.384 ± 0.065
1.068LeuTrp: 1.068 ± 0.022
3.191LeuTyr: 3.191 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.54MetAla: 2.54 ± 0.033
0.137MetCys: 0.137 ± 0.008
1.427MetAsp: 1.427 ± 0.024
1.56MetGlu: 1.56 ± 0.026
0.95MetPhe: 0.95 ± 0.022
1.631MetGly: 1.631 ± 0.035
0.497MetHis: 0.497 ± 0.019
1.577MetIle: 1.577 ± 0.033
1.674MetLys: 1.674 ± 0.03
2.887MetLeu: 2.887 ± 0.041
0.766MetMet: 0.766 ± 0.022
1.29MetAsn: 1.29 ± 0.026
1.265MetPro: 1.265 ± 0.026
0.868MetGln: 0.868 ± 0.022
1.617MetArg: 1.617 ± 0.036
1.551MetSer: 1.551 ± 0.025
1.783MetThr: 1.783 ± 0.028
1.409MetVal: 1.409 ± 0.028
0.229MetTrp: 0.229 ± 0.01
0.677MetTyr: 0.677 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.555AsnAla: 3.555 ± 0.047
0.212AsnCys: 0.212 ± 0.011
1.768AsnAsp: 1.768 ± 0.033
2.089AsnGlu: 2.089 ± 0.03
1.199AsnPhe: 1.199 ± 0.027
3.506AsnGly: 3.506 ± 0.056
0.681AsnHis: 0.681 ± 0.017
1.65AsnIle: 1.65 ± 0.034
1.495AsnLys: 1.495 ± 0.028
2.878AsnLeu: 2.878 ± 0.043
0.846AsnMet: 0.846 ± 0.021
1.224AsnAsn: 1.224 ± 0.035
1.885AsnPro: 1.885 ± 0.031
1.122AsnGln: 1.122 ± 0.027
2.07AsnArg: 2.07 ± 0.033
1.461AsnSer: 1.461 ± 0.031
1.613AsnThr: 1.613 ± 0.033
2.516AsnVal: 2.516 ± 0.038
0.471AsnTrp: 0.471 ± 0.015
1.16AsnTyr: 1.16 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
5.091ProAla: 5.091 ± 0.07
0.256ProCys: 0.256 ± 0.012
3.153ProAsp: 3.153 ± 0.043
3.468ProGlu: 3.468 ± 0.047
2.065ProPhe: 2.065 ± 0.027
3.832ProGly: 3.832 ± 0.047
0.944ProHis: 0.944 ± 0.024
2.395ProIle: 2.395 ± 0.033
1.736ProLys: 1.736 ± 0.028
4.309ProLeu: 4.309 ± 0.049
1.093ProMet: 1.093 ± 0.027
1.59ProAsn: 1.59 ± 0.037
1.641ProPro: 1.641 ± 0.036
1.4ProGln: 1.4 ± 0.036
1.993ProArg: 1.993 ± 0.035
2.608ProSer: 2.608 ± 0.034
1.912ProThr: 1.912 ± 0.036
3.497ProVal: 3.497 ± 0.044
0.581ProTrp: 0.581 ± 0.018
1.553ProTyr: 1.553 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.801GlnAla: 3.801 ± 0.05
0.152GlnCys: 0.152 ± 0.009
1.547GlnAsp: 1.547 ± 0.027
1.96GlnGlu: 1.96 ± 0.035
1.336GlnPhe: 1.336 ± 0.025
2.496GlnGly: 2.496 ± 0.054
0.673GlnHis: 0.673 ± 0.019
1.847GlnIle: 1.847 ± 0.031
1.341GlnLys: 1.341 ± 0.027
3.732GlnLeu: 3.732 ± 0.047
0.844GlnMet: 0.844 ± 0.021
1.041GlnAsn: 1.041 ± 0.021
1.588GlnPro: 1.588 ± 0.036
1.411GlnGln: 1.411 ± 0.036
1.896GlnArg: 1.896 ± 0.036
1.88GlnSer: 1.88 ± 0.036
1.832GlnThr: 1.832 ± 0.029
2.083GlnVal: 2.083 ± 0.035
0.48GlnTrp: 0.48 ± 0.015
1.126GlnTyr: 1.126 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
5.393ArgAla: 5.393 ± 0.07
0.417ArgCys: 0.417 ± 0.016
3.096ArgAsp: 3.096 ± 0.042
4.435ArgGlu: 4.435 ± 0.063
2.736ArgPhe: 2.736 ± 0.045
4.17ArgGly: 4.17 ± 0.058
1.344ArgHis: 1.344 ± 0.028
3.966ArgIle: 3.966 ± 0.049
2.94ArgLys: 2.94 ± 0.042
6.698ArgLeu: 6.698 ± 0.075
1.947ArgMet: 1.947 ± 0.034
1.958ArgAsn: 1.958 ± 0.032
2.541ArgPro: 2.541 ± 0.039
2.228ArgGln: 2.228 ± 0.035
4.131ArgArg: 4.131 ± 0.058
3.348ArgSer: 3.348 ± 0.043
3.242ArgThr: 3.242 ± 0.044
3.68ArgVal: 3.68 ± 0.049
0.822ArgTrp: 0.822 ± 0.023
2.178ArgTyr: 2.178 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
5.574SerAla: 5.574 ± 0.061
0.381SerCys: 0.381 ± 0.014
2.882SerAsp: 2.882 ± 0.042
3.018SerGlu: 3.018 ± 0.039
2.617SerPhe: 2.617 ± 0.037
5.45SerGly: 5.45 ± 0.062
1.051SerHis: 1.051 ± 0.023
3.13SerIle: 3.13 ± 0.04
2.401SerLys: 2.401 ± 0.04
5.682SerLeu: 5.682 ± 0.063
1.474SerMet: 1.474 ± 0.027
1.8SerAsn: 1.8 ± 0.037
2.568SerPro: 2.568 ± 0.039
1.588SerGln: 1.588 ± 0.032
3.214SerArg: 3.214 ± 0.043
3.282SerSer: 3.282 ± 0.051
2.586SerThr: 2.586 ± 0.035
4.165SerVal: 4.165 ± 0.053
0.788SerTrp: 0.788 ± 0.022
1.921SerTyr: 1.921 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
5.236ThrAla: 5.236 ± 0.058
0.319ThrCys: 0.319 ± 0.012
2.864ThrAsp: 2.864 ± 0.043
2.803ThrGlu: 2.803 ± 0.039
2.286ThrPhe: 2.286 ± 0.038
4.685ThrGly: 4.685 ± 0.072
0.936ThrHis: 0.936 ± 0.025
3.313ThrIle: 3.313 ± 0.039
2.11ThrLys: 2.11 ± 0.033
5.104ThrLeu: 5.104 ± 0.056
1.345ThrMet: 1.345 ± 0.025
1.834ThrAsn: 1.834 ± 0.034
2.576ThrPro: 2.576 ± 0.041
1.328ThrGln: 1.328 ± 0.028
2.364ThrArg: 2.364 ± 0.037
2.741ThrSer: 2.741 ± 0.043
2.678ThrThr: 2.678 ± 0.049
4.451ThrVal: 4.451 ± 0.063
0.618ThrTrp: 0.618 ± 0.019
1.796ThrTyr: 1.796 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
6.411ValAla: 6.411 ± 0.061
0.634ValCys: 0.634 ± 0.018
3.686ValAsp: 3.686 ± 0.041
3.83ValGlu: 3.83 ± 0.045
2.93ValPhe: 2.93 ± 0.045
4.905ValGly: 4.905 ± 0.048
1.421ValHis: 1.421 ± 0.024
4.083ValIle: 4.083 ± 0.047
3.478ValLys: 3.478 ± 0.044
7.201ValLeu: 7.201 ± 0.069
1.852ValMet: 1.852 ± 0.03
2.591ValAsn: 2.591 ± 0.038
3.578ValPro: 3.578 ± 0.049
2.292ValGln: 2.292 ± 0.035
4.405ValArg: 4.405 ± 0.053
4.334ValSer: 4.334 ± 0.052
4.376ValThr: 4.376 ± 0.067
5.42ValVal: 5.42 ± 0.064
0.893ValTrp: 0.893 ± 0.026
2.397ValTyr: 2.397 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.015TrpAla: 1.015 ± 0.022
0.116TrpCys: 0.116 ± 0.007
0.726TrpAsp: 0.726 ± 0.022
0.712TrpGlu: 0.712 ± 0.017
0.576TrpPhe: 0.576 ± 0.018
0.932TrpGly: 0.932 ± 0.026
0.324TrpHis: 0.324 ± 0.014
0.803TrpIle: 0.803 ± 0.019
0.641TrpLys: 0.641 ± 0.018
1.535TrpLeu: 1.535 ± 0.032
0.395TrpMet: 0.395 ± 0.014
0.63TrpAsn: 0.63 ± 0.019
0.474TrpPro: 0.474 ± 0.017
0.518TrpGln: 0.518 ± 0.017
0.828TrpArg: 0.828 ± 0.022
0.845TrpSer: 0.845 ± 0.021
0.876TrpThr: 0.876 ± 0.022
0.799TrpVal: 0.799 ± 0.019
0.19TrpTrp: 0.19 ± 0.01
0.41TrpTyr: 0.41 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.459TyrAla: 3.459 ± 0.047
0.258TyrCys: 0.258 ± 0.012
1.958TyrAsp: 1.958 ± 0.043
2.101TyrGlu: 2.101 ± 0.036
1.492TyrPhe: 1.492 ± 0.028
2.86TyrGly: 2.86 ± 0.037
0.637TyrHis: 0.637 ± 0.017
1.605TyrIle: 1.605 ± 0.03
1.392TyrLys: 1.392 ± 0.032
3.226TyrLeu: 3.226 ± 0.042
0.84TyrMet: 0.84 ± 0.022
1.181TyrAsn: 1.181 ± 0.025
1.555TyrPro: 1.555 ± 0.032
0.962TyrGln: 0.962 ± 0.023
2.322TyrArg: 2.322 ± 0.035
1.653TyrSer: 1.653 ± 0.032
1.609TyrThr: 1.609 ± 0.03
2.422TyrVal: 2.422 ± 0.034
0.503TyrTrp: 0.503 ± 0.016
1.301TyrTyr: 1.301 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.002
Statistics based on 6505 proteins (2093348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski