Burkholderia phage phiE058

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B8E109|A0A3B8E109_9CAUD Uncharacterized protein OS=Burkholderia phage phiE058 OX=2321389 GN=phiE058_023 PE=4 SV=1
MM1 pKa = 7.8PVTISITEE9 pKa = 4.25SQVFTALRR17 pKa = 11.84SFLLGILPAGVEE29 pKa = 4.39VVKK32 pKa = 10.99AQGNGVGEE40 pKa = 4.12PVGGDD45 pKa = 3.11FVVMNSIASPRR56 pKa = 11.84IATNVEE62 pKa = 4.71GYY64 pKa = 8.43TDD66 pKa = 4.13PGTNPGTRR74 pKa = 11.84NSMQAIEE81 pKa = 5.56ARR83 pKa = 11.84IQLNVHH89 pKa = 6.4GPNSGDD95 pKa = 3.08NAAIISTLFRR105 pKa = 11.84DD106 pKa = 4.72EE107 pKa = 4.24YY108 pKa = 11.6ACIQFATVNPDD119 pKa = 2.96IQPLYY124 pKa = 11.0SEE126 pKa = 5.65IPRR129 pKa = 11.84QMPFINGEE137 pKa = 3.97NQFEE141 pKa = 4.18QRR143 pKa = 11.84WIIEE147 pKa = 3.91LALQYY152 pKa = 11.24NPITQTPQDD161 pKa = 3.74FADD164 pKa = 3.73EE165 pKa = 4.71VIPQIVSVDD174 pKa = 3.13AAYY177 pKa = 9.5PPGAA181 pKa = 4.3

Molecular weight: 19.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B8E1H6|A0A3B8E1H6_9CAUD Uncharacterized protein OS=Burkholderia phage phiE058 OX=2321389 GN=phiE058_040 PE=4 SV=1
MM1 pKa = 7.73LDD3 pKa = 2.99IFQVVTHH10 pKa = 6.2CLITLFKK17 pKa = 10.42HH18 pKa = 6.1RR19 pKa = 11.84NPIRR23 pKa = 11.84KK24 pKa = 8.45PRR26 pKa = 11.84IKK28 pKa = 10.71ANTPRR33 pKa = 11.84RR34 pKa = 11.84SPYY37 pKa = 10.34QRR39 pKa = 11.84GQDD42 pKa = 3.59GNEE45 pKa = 3.79TGDD48 pKa = 3.53RR49 pKa = 11.84TNHH52 pKa = 5.91GFCTYY57 pKa = 10.47KK58 pKa = 10.48PRR60 pKa = 11.84TGAILLVILTIHH72 pKa = 7.22LIEE75 pKa = 4.51PVNN78 pKa = 3.4

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13989

41

759

199.8

21.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.075 ± 0.513

0.922 ± 0.111

5.74 ± 0.292

5.633 ± 0.521

3.131 ± 0.172

8.199 ± 0.358

1.809 ± 0.195

5.09 ± 0.23

3.76 ± 0.257

7.62 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.137

3.789 ± 0.244

5.247 ± 0.269

4.411 ± 0.225

6.348 ± 0.448

5.797 ± 0.426

6.305 ± 0.307

6.877 ± 0.285

1.465 ± 0.12

2.359 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski