Amino acid dipepetide frequency for Burkholderia phage phiE058

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.517AlaAla: 19.517 ± 2.138
1.215AlaCys: 1.215 ± 0.323
7.149AlaAsp: 7.149 ± 0.695
9.008AlaGlu: 9.008 ± 1.647
4.075AlaPhe: 4.075 ± 0.549
9.866AlaGly: 9.866 ± 0.932
1.43AlaHis: 1.43 ± 0.266
5.862AlaIle: 5.862 ± 0.723
4.432AlaLys: 4.432 ± 0.532
10.58AlaLeu: 10.58 ± 0.971
4.432AlaMet: 4.432 ± 0.529
4.289AlaAsn: 4.289 ± 0.692
5.648AlaPro: 5.648 ± 0.631
6.577AlaGln: 6.577 ± 0.827
9.008AlaArg: 9.008 ± 1.062
8.15AlaSer: 8.15 ± 0.871
6.649AlaThr: 6.649 ± 0.895
8.007AlaVal: 8.007 ± 0.869
2.073AlaTrp: 2.073 ± 0.393
2.788AlaTyr: 2.788 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.284
0.143CysCys: 0.143 ± 0.147
0.715CysAsp: 0.715 ± 0.24
0.357CysGlu: 0.357 ± 0.165
0.071CysPhe: 0.071 ± 0.089
1.501CysGly: 1.501 ± 0.293
0.357CysHis: 0.357 ± 0.169
0.429CysIle: 0.429 ± 0.193
0.429CysLys: 0.429 ± 0.165
0.643CysLeu: 0.643 ± 0.201
0.214CysMet: 0.214 ± 0.143
0.286CysAsn: 0.286 ± 0.163
0.286CysPro: 0.286 ± 0.151
0.214CysGln: 0.214 ± 0.123
0.786CysArg: 0.786 ± 0.269
0.357CysSer: 0.357 ± 0.153
0.429CysThr: 0.429 ± 0.192
0.858CysVal: 0.858 ± 0.276
0.143CysTrp: 0.143 ± 0.107
0.214CysTyr: 0.214 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
8.722AspAla: 8.722 ± 1.139
0.357AspCys: 0.357 ± 0.138
3.074AspAsp: 3.074 ± 0.51
4.575AspGlu: 4.575 ± 0.696
1.501AspPhe: 1.501 ± 0.326
5.719AspGly: 5.719 ± 0.508
1.287AspHis: 1.287 ± 0.251
2.574AspIle: 2.574 ± 0.378
1.573AspLys: 1.573 ± 0.269
4.861AspLeu: 4.861 ± 0.599
1.43AspMet: 1.43 ± 0.378
2.717AspAsn: 2.717 ± 0.455
3.146AspPro: 3.146 ± 0.416
1.501AspGln: 1.501 ± 0.38
3.789AspArg: 3.789 ± 0.435
2.645AspSer: 2.645 ± 0.417
2.788AspThr: 2.788 ± 0.334
3.432AspVal: 3.432 ± 0.453
1.215AspTrp: 1.215 ± 0.297
0.5AspTyr: 0.5 ± 0.211
0.0AspXaa: 0.0 ± 0.0
Glu
7.578GluAla: 7.578 ± 1.127
0.286GluCys: 0.286 ± 0.145
2.431GluAsp: 2.431 ± 0.441
3.86GluGlu: 3.86 ± 0.795
2.002GluPhe: 2.002 ± 0.335
3.146GluGly: 3.146 ± 0.429
1.43GluHis: 1.43 ± 0.359
4.218GluIle: 4.218 ± 0.55
2.216GluLys: 2.216 ± 0.506
4.647GluLeu: 4.647 ± 0.662
1.43GluMet: 1.43 ± 0.368
1.358GluAsn: 1.358 ± 0.313
2.502GluPro: 2.502 ± 0.473
3.289GluGln: 3.289 ± 0.59
4.647GluArg: 4.647 ± 1.205
3.146GluSer: 3.146 ± 0.43
2.931GluThr: 2.931 ± 0.494
3.074GluVal: 3.074 ± 0.466
0.929GluTrp: 0.929 ± 0.215
1.215GluTyr: 1.215 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
4.003PheAla: 4.003 ± 0.387
0.286PheCys: 0.286 ± 0.128
2.86PheAsp: 2.86 ± 0.378
2.073PheGlu: 2.073 ± 0.339
1.001PhePhe: 1.001 ± 0.256
3.503PheGly: 3.503 ± 0.419
0.5PheHis: 0.5 ± 0.207
1.215PheIle: 1.215 ± 0.282
1.144PheLys: 1.144 ± 0.38
2.073PheLeu: 2.073 ± 0.445
0.643PheMet: 0.643 ± 0.2
1.287PheAsn: 1.287 ± 0.383
1.644PhePro: 1.644 ± 0.279
0.429PheGln: 0.429 ± 0.177
1.787PheArg: 1.787 ± 0.347
1.573PheSer: 1.573 ± 0.295
2.002PheThr: 2.002 ± 0.468
2.002PheVal: 2.002 ± 0.41
0.715PheTrp: 0.715 ± 0.22
0.572PheTyr: 0.572 ± 0.357
0.0PheXaa: 0.0 ± 0.0
Gly
9.079GlyAla: 9.079 ± 0.974
1.358GlyCys: 1.358 ± 0.357
5.505GlyAsp: 5.505 ± 0.886
3.503GlyGlu: 3.503 ± 0.463
3.646GlyPhe: 3.646 ± 0.392
7.792GlyGly: 7.792 ± 1.056
0.786GlyHis: 0.786 ± 0.268
4.075GlyIle: 4.075 ± 0.569
3.074GlyLys: 3.074 ± 0.503
5.362GlyLeu: 5.362 ± 0.575
2.359GlyMet: 2.359 ± 0.367
3.146GlyAsn: 3.146 ± 0.405
2.502GlyPro: 2.502 ± 0.357
2.86GlyGln: 2.86 ± 0.382
5.362GlyArg: 5.362 ± 0.635
4.504GlySer: 4.504 ± 0.708
5.505GlyThr: 5.505 ± 0.764
7.506GlyVal: 7.506 ± 0.976
0.786GlyTrp: 0.786 ± 0.206
3.289GlyTyr: 3.289 ± 0.463
0.0GlyXaa: 0.0 ± 0.0
His
2.431HisAla: 2.431 ± 0.4
0.286HisCys: 0.286 ± 0.148
1.001HisAsp: 1.001 ± 0.244
0.715HisGlu: 0.715 ± 0.233
0.929HisPhe: 0.929 ± 0.296
1.859HisGly: 1.859 ± 0.373
0.5HisHis: 0.5 ± 0.175
0.858HisIle: 0.858 ± 0.319
0.643HisLys: 0.643 ± 0.182
1.358HisLeu: 1.358 ± 0.328
0.357HisMet: 0.357 ± 0.148
0.572HisAsn: 0.572 ± 0.22
1.287HisPro: 1.287 ± 0.411
0.357HisGln: 0.357 ± 0.167
1.144HisArg: 1.144 ± 0.379
0.572HisSer: 0.572 ± 0.209
0.858HisThr: 0.858 ± 0.25
1.716HisVal: 1.716 ± 0.284
0.357HisTrp: 0.357 ± 0.19
0.286HisTyr: 0.286 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
7.006IleAla: 7.006 ± 0.725
0.286IleCys: 0.286 ± 0.157
3.074IleAsp: 3.074 ± 0.5
3.932IleGlu: 3.932 ± 0.53
1.358IlePhe: 1.358 ± 0.336
4.218IleGly: 4.218 ± 0.551
0.5IleHis: 0.5 ± 0.157
2.002IleIle: 2.002 ± 0.361
2.359IleLys: 2.359 ± 0.372
2.502IleLeu: 2.502 ± 0.34
0.429IleMet: 0.429 ± 0.161
1.93IleAsn: 1.93 ± 0.364
3.074IlePro: 3.074 ± 0.41
2.288IleGln: 2.288 ± 0.445
3.074IleArg: 3.074 ± 0.479
2.931IleSer: 2.931 ± 0.504
4.289IleThr: 4.289 ± 0.493
3.86IleVal: 3.86 ± 0.547
0.858IleTrp: 0.858 ± 0.301
0.929IleTyr: 0.929 ± 0.262
0.0IleXaa: 0.0 ± 0.0
Lys
5.576LysAla: 5.576 ± 0.638
0.214LysCys: 0.214 ± 0.13
1.501LysAsp: 1.501 ± 0.374
1.573LysGlu: 1.573 ± 0.416
1.215LysPhe: 1.215 ± 0.304
1.93LysGly: 1.93 ± 0.369
1.001LysHis: 1.001 ± 0.311
2.431LysIle: 2.431 ± 0.413
2.002LysLys: 2.002 ± 0.374
3.074LysLeu: 3.074 ± 0.518
0.786LysMet: 0.786 ± 0.267
1.573LysAsn: 1.573 ± 0.353
2.359LysPro: 2.359 ± 0.415
1.501LysGln: 1.501 ± 0.327
2.431LysArg: 2.431 ± 0.432
1.501LysSer: 1.501 ± 0.269
2.645LysThr: 2.645 ± 0.426
2.502LysVal: 2.502 ± 0.407
1.001LysTrp: 1.001 ± 0.282
0.786LysTyr: 0.786 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
10.438LeuAla: 10.438 ± 0.769
0.643LeuCys: 0.643 ± 0.213
3.574LeuAsp: 3.574 ± 0.487
3.86LeuGlu: 3.86 ± 0.565
2.359LeuPhe: 2.359 ± 0.404
5.219LeuGly: 5.219 ± 0.628
1.573LeuHis: 1.573 ± 0.381
4.289LeuIle: 4.289 ± 0.467
3.289LeuLys: 3.289 ± 0.666
6.148LeuLeu: 6.148 ± 0.702
1.358LeuMet: 1.358 ± 0.292
2.788LeuAsn: 2.788 ± 0.482
3.574LeuPro: 3.574 ± 0.503
3.646LeuGln: 3.646 ± 0.602
4.79LeuArg: 4.79 ± 0.589
5.219LeuSer: 5.219 ± 0.768
6.434LeuThr: 6.434 ± 0.71
4.647LeuVal: 4.647 ± 0.601
0.715LeuTrp: 0.715 ± 0.207
1.644LeuTyr: 1.644 ± 0.339
0.0LeuXaa: 0.0 ± 0.0
Met
3.289MetAla: 3.289 ± 0.476
0.071MetCys: 0.071 ± 0.077
1.716MetAsp: 1.716 ± 0.367
0.643MetGlu: 0.643 ± 0.192
0.572MetPhe: 0.572 ± 0.205
1.215MetGly: 1.215 ± 0.315
0.643MetHis: 0.643 ± 0.246
0.715MetIle: 0.715 ± 0.26
1.43MetLys: 1.43 ± 0.262
2.288MetLeu: 2.288 ± 0.34
0.643MetMet: 0.643 ± 0.203
0.858MetAsn: 0.858 ± 0.252
1.501MetPro: 1.501 ± 0.284
1.072MetGln: 1.072 ± 0.372
2.216MetArg: 2.216 ± 0.455
2.502MetSer: 2.502 ± 0.349
1.287MetThr: 1.287 ± 0.277
1.287MetVal: 1.287 ± 0.305
0.071MetTrp: 0.071 ± 0.063
0.214MetTyr: 0.214 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
4.218AsnAla: 4.218 ± 0.507
0.357AsnCys: 0.357 ± 0.155
2.073AsnAsp: 2.073 ± 0.369
1.644AsnGlu: 1.644 ± 0.309
1.144AsnPhe: 1.144 ± 0.215
4.003AsnGly: 4.003 ± 0.612
0.429AsnHis: 0.429 ± 0.175
1.93AsnIle: 1.93 ± 0.379
0.786AsnLys: 0.786 ± 0.325
2.431AsnLeu: 2.431 ± 0.395
1.072AsnMet: 1.072 ± 0.301
1.287AsnAsn: 1.287 ± 0.323
2.788AsnPro: 2.788 ± 0.473
1.43AsnGln: 1.43 ± 0.306
2.002AsnArg: 2.002 ± 0.389
2.574AsnSer: 2.574 ± 0.557
2.431AsnThr: 2.431 ± 0.478
3.289AsnVal: 3.289 ± 0.488
0.929AsnTrp: 0.929 ± 0.246
1.072AsnTyr: 1.072 ± 0.375
0.0AsnXaa: 0.0 ± 0.0
Pro
7.649ProAla: 7.649 ± 0.81
0.286ProCys: 0.286 ± 0.144
2.788ProAsp: 2.788 ± 0.503
3.003ProGlu: 3.003 ± 0.457
1.215ProPhe: 1.215 ± 0.323
4.432ProGly: 4.432 ± 0.551
1.287ProHis: 1.287 ± 0.346
2.931ProIle: 2.931 ± 0.336
1.859ProLys: 1.859 ± 0.426
3.36ProLeu: 3.36 ± 0.58
0.929ProMet: 0.929 ± 0.261
2.645ProAsn: 2.645 ± 0.427
2.574ProPro: 2.574 ± 0.508
2.073ProGln: 2.073 ± 0.369
3.217ProArg: 3.217 ± 0.56
2.431ProSer: 2.431 ± 0.578
4.218ProThr: 4.218 ± 0.463
3.503ProVal: 3.503 ± 0.491
0.5ProTrp: 0.5 ± 0.174
1.573ProTyr: 1.573 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
5.219GlnAla: 5.219 ± 0.795
0.5GlnCys: 0.5 ± 0.18
1.501GlnAsp: 1.501 ± 0.417
1.859GlnGlu: 1.859 ± 0.459
1.501GlnPhe: 1.501 ± 0.318
3.574GlnGly: 3.574 ± 0.548
1.072GlnHis: 1.072 ± 0.295
2.073GlnIle: 2.073 ± 0.43
1.287GlnLys: 1.287 ± 0.283
3.574GlnLeu: 3.574 ± 0.528
1.787GlnMet: 1.787 ± 0.425
2.145GlnAsn: 2.145 ± 0.467
2.574GlnPro: 2.574 ± 0.492
2.86GlnGln: 2.86 ± 0.549
2.645GlnArg: 2.645 ± 0.495
2.502GlnSer: 2.502 ± 0.41
2.288GlnThr: 2.288 ± 0.348
3.217GlnVal: 3.217 ± 0.517
0.715GlnTrp: 0.715 ± 0.207
1.215GlnTyr: 1.215 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
9.508ArgAla: 9.508 ± 1.178
0.786ArgCys: 0.786 ± 0.261
4.075ArgAsp: 4.075 ± 0.595
4.504ArgGlu: 4.504 ± 1.154
2.216ArgPhe: 2.216 ± 0.425
4.003ArgGly: 4.003 ± 0.568
1.43ArgHis: 1.43 ± 0.342
3.717ArgIle: 3.717 ± 0.447
2.645ArgLys: 2.645 ± 0.495
4.718ArgLeu: 4.718 ± 0.562
1.43ArgMet: 1.43 ± 0.319
2.073ArgAsn: 2.073 ± 0.34
2.931ArgPro: 2.931 ± 0.441
2.216ArgGln: 2.216 ± 0.426
4.504ArgArg: 4.504 ± 0.675
2.931ArgSer: 2.931 ± 0.425
3.217ArgThr: 3.217 ± 0.477
3.432ArgVal: 3.432 ± 0.486
1.144ArgTrp: 1.144 ± 0.294
2.002ArgTyr: 2.002 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
7.292SerAla: 7.292 ± 1.308
0.786SerCys: 0.786 ± 0.245
3.646SerAsp: 3.646 ± 0.494
2.288SerGlu: 2.288 ± 0.394
1.716SerPhe: 1.716 ± 0.386
5.719SerGly: 5.719 ± 0.986
1.144SerHis: 1.144 ± 0.288
3.432SerIle: 3.432 ± 0.448
1.93SerLys: 1.93 ± 0.313
3.789SerLeu: 3.789 ± 0.844
1.644SerMet: 1.644 ± 0.337
2.288SerAsn: 2.288 ± 0.401
2.574SerPro: 2.574 ± 0.506
3.003SerGln: 3.003 ± 0.508
2.788SerArg: 2.788 ± 0.425
3.717SerSer: 3.717 ± 0.909
4.361SerThr: 4.361 ± 0.451
4.718SerVal: 4.718 ± 0.81
0.071SerTrp: 0.071 ± 0.065
1.072SerTyr: 1.072 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
7.292ThrAla: 7.292 ± 0.663
0.286ThrCys: 0.286 ± 0.14
3.289ThrAsp: 3.289 ± 0.489
2.645ThrGlu: 2.645 ± 0.514
1.644ThrPhe: 1.644 ± 0.411
6.005ThrGly: 6.005 ± 0.655
0.786ThrHis: 0.786 ± 0.255
3.86ThrIle: 3.86 ± 0.648
2.431ThrLys: 2.431 ± 0.541
5.219ThrLeu: 5.219 ± 0.604
0.858ThrMet: 0.858 ± 0.314
2.288ThrAsn: 2.288 ± 0.445
5.076ThrPro: 5.076 ± 0.632
3.003ThrGln: 3.003 ± 0.409
3.289ThrArg: 3.289 ± 0.426
3.717ThrSer: 3.717 ± 0.612
3.717ThrThr: 3.717 ± 0.546
5.29ThrVal: 5.29 ± 0.674
1.001ThrTrp: 1.001 ± 0.274
1.358ThrTyr: 1.358 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
7.578ValAla: 7.578 ± 0.806
0.5ValCys: 0.5 ± 0.179
5.004ValAsp: 5.004 ± 0.589
3.789ValGlu: 3.789 ± 0.489
1.787ValPhe: 1.787 ± 0.351
5.076ValGly: 5.076 ± 0.633
1.573ValHis: 1.573 ± 0.315
2.502ValIle: 2.502 ± 0.418
2.359ValLys: 2.359 ± 0.363
5.648ValLeu: 5.648 ± 0.619
1.93ValMet: 1.93 ± 0.342
2.502ValAsn: 2.502 ± 0.41
4.146ValPro: 4.146 ± 0.69
4.432ValGln: 4.432 ± 0.643
4.003ValArg: 4.003 ± 0.639
4.361ValSer: 4.361 ± 0.573
4.79ValThr: 4.79 ± 0.728
4.218ValVal: 4.218 ± 0.618
0.858ValTrp: 0.858 ± 0.244
1.716ValTyr: 1.716 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
1.215TrpAla: 1.215 ± 0.245
0.286TrpCys: 0.286 ± 0.145
1.072TrpAsp: 1.072 ± 0.217
1.144TrpGlu: 1.144 ± 0.264
0.429TrpPhe: 0.429 ± 0.156
0.929TrpGly: 0.929 ± 0.285
0.214TrpHis: 0.214 ± 0.155
0.929TrpIle: 0.929 ± 0.251
0.929TrpLys: 0.929 ± 0.286
1.501TrpLeu: 1.501 ± 0.376
0.0TrpMet: 0.0 ± 0.0
0.786TrpAsn: 0.786 ± 0.226
0.572TrpPro: 0.572 ± 0.167
0.715TrpGln: 0.715 ± 0.2
0.715TrpArg: 0.715 ± 0.233
1.001TrpSer: 1.001 ± 0.226
0.929TrpThr: 0.929 ± 0.285
0.572TrpVal: 0.572 ± 0.212
0.214TrpTrp: 0.214 ± 0.128
0.5TrpTyr: 0.5 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.431TyrAla: 2.431 ± 0.389
0.286TyrCys: 0.286 ± 0.146
1.358TyrAsp: 1.358 ± 0.351
1.358TyrGlu: 1.358 ± 0.283
0.929TyrPhe: 0.929 ± 0.22
2.288TyrGly: 2.288 ± 0.474
0.143TyrHis: 0.143 ± 0.106
0.858TyrIle: 0.858 ± 0.269
0.786TyrLys: 0.786 ± 0.233
2.431TyrLeu: 2.431 ± 0.411
0.429TyrMet: 0.429 ± 0.16
1.072TyrAsn: 1.072 ± 0.258
1.716TyrPro: 1.716 ± 0.378
0.643TyrGln: 0.643 ± 0.23
1.287TyrArg: 1.287 ± 0.27
1.644TyrSer: 1.644 ± 0.401
1.287TyrThr: 1.287 ± 0.261
1.644TyrVal: 1.644 ± 0.315
0.357TyrTrp: 0.357 ± 0.146
0.643TyrTyr: 0.643 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (13989 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski