Magnaporthiopsis poae (strain ATCC 64411 / 73-15) (Kentucky bluegrass fungus) (Magnaporthe poae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Magnaporthales; Magnaporthaceae; Magnaporthiopsis; Magnaporthiopsis poae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4ED00|A0A0C4ED00_MAGP6 Uncharacterized protein OS=Magnaporthiopsis poae (strain ATCC 64411 / 73-15) OX=644358 GN=MAPG_10591 PE=4 SV=1
MM1 pKa = 7.08EE2 pKa = 5.73HH3 pKa = 6.95SSGMWYY9 pKa = 10.07DD10 pKa = 3.4GRR12 pKa = 11.84EE13 pKa = 4.05LDD15 pKa = 5.06SGTLEE20 pKa = 4.1NVDD23 pKa = 3.92YY24 pKa = 11.14QHH26 pKa = 6.95VDD28 pKa = 3.52SPCGRR33 pKa = 11.84SGALFMCYY41 pKa = 10.07YY42 pKa = 10.71DD43 pKa = 3.35GRR45 pKa = 11.84LYY47 pKa = 10.96ADD49 pKa = 4.32SEE51 pKa = 4.52ACYY54 pKa = 10.58SDD56 pKa = 4.64EE57 pKa = 6.36DD58 pKa = 4.79EE59 pKa = 5.87DD60 pKa = 6.47DD61 pKa = 3.81EE62 pKa = 6.57DD63 pKa = 5.4LDD65 pKa = 5.06EE66 pKa = 6.09DD67 pKa = 3.96VLLYY71 pKa = 11.2GEE73 pKa = 5.66DD74 pKa = 3.57EE75 pKa = 4.85ADD77 pKa = 3.54YY78 pKa = 11.55DD79 pKa = 5.19DD80 pKa = 6.78DD81 pKa = 5.38NGTDD85 pKa = 3.53GAATNEE91 pKa = 3.94YY92 pKa = 10.63DD93 pKa = 5.01DD94 pKa = 4.16EE95 pKa = 4.7QEE97 pKa = 4.63EE98 pKa = 4.09EE99 pKa = 4.4DD100 pKa = 4.85ADD102 pKa = 4.21SEE104 pKa = 4.84SPDD107 pKa = 4.3DD108 pKa = 4.67EE109 pKa = 4.37EE110 pKa = 4.43LLYY113 pKa = 10.24MRR115 pKa = 11.84YY116 pKa = 10.11CSDD119 pKa = 3.92DD120 pKa = 3.29AAKK123 pKa = 10.28RR124 pKa = 11.84PAVVEE129 pKa = 4.23HH130 pKa = 6.31EE131 pKa = 5.02LEE133 pKa = 3.97FDD135 pKa = 3.44MSKK138 pKa = 11.13LSLDD142 pKa = 3.46QFKK145 pKa = 10.88FEE147 pKa = 5.13HH148 pKa = 6.69NMMSSPLDD156 pKa = 3.88PNDD159 pKa = 3.17LHH161 pKa = 6.46GRR163 pKa = 11.84LMLVQGRR170 pKa = 11.84EE171 pKa = 4.09GG172 pKa = 3.17

Molecular weight: 19.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4DL31|A0A0C4DL31_MAGP6 Uncharacterized protein OS=Magnaporthiopsis poae (strain ATCC 64411 / 73-15) OX=644358 GN=MAPG_00466 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11206

97

11303

4899822

31

6263

433.5

47.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.884 ± 0.025

1.341 ± 0.009

5.748 ± 0.019

5.724 ± 0.027

3.445 ± 0.016

7.625 ± 0.024

2.413 ± 0.01

4.072 ± 0.015

4.397 ± 0.024

8.65 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.009

3.182 ± 0.012

6.675 ± 0.028

3.976 ± 0.02

6.874 ± 0.023

8.009 ± 0.026

5.706 ± 0.022

6.22 ± 0.016

1.471 ± 0.009

2.424 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski