Amino acid dipepetide frequency for Paramecium bursaria Chlorella virus MT325 (PBCV-MT325)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.852AlaAla: 4.852 ± 0.246
1.179AlaCys: 1.179 ± 0.098
2.972AlaAsp: 2.972 ± 0.163
2.743AlaGlu: 2.743 ± 0.17
3.06AlaPhe: 3.06 ± 0.139
4.899AlaGly: 4.899 ± 0.501
1.409AlaHis: 1.409 ± 0.127
4.428AlaIle: 4.428 ± 0.158
3.74AlaLys: 3.74 ± 0.23
5.6AlaLeu: 5.6 ± 0.241
2.015AlaMet: 2.015 ± 0.133
3.552AlaAsn: 3.552 ± 0.368
4.596AlaPro: 4.596 ± 0.494
1.793AlaGln: 1.793 ± 0.103
3.572AlaArg: 3.572 ± 0.174
5.614AlaSer: 5.614 ± 0.296
3.801AlaThr: 3.801 ± 0.185
4.044AlaVal: 4.044 ± 0.18
0.728AlaTrp: 0.728 ± 0.069
2.157AlaTyr: 2.157 ± 0.158
0.0AlaXaa: 0.0 ± 0.0
Cys
1.307CysAla: 1.307 ± 0.087
0.64CysCys: 0.64 ± 0.082
0.903CysAsp: 0.903 ± 0.08
0.613CysGlu: 0.613 ± 0.068
1.092CysPhe: 1.092 ± 0.089
1.2CysGly: 1.2 ± 0.114
0.714CysHis: 0.714 ± 0.085
1.267CysIle: 1.267 ± 0.108
1.004CysLys: 1.004 ± 0.114
1.82CysLeu: 1.82 ± 0.137
0.613CysMet: 0.613 ± 0.074
0.755CysAsn: 0.755 ± 0.077
1.24CysPro: 1.24 ± 0.102
0.795CysGln: 0.795 ± 0.088
1.072CysArg: 1.072 ± 0.112
1.692CysSer: 1.692 ± 0.142
0.923CysThr: 0.923 ± 0.085
1.388CysVal: 1.388 ± 0.114
0.364CysTrp: 0.364 ± 0.063
0.445CysTyr: 0.445 ± 0.053
0.0CysXaa: 0.0 ± 0.0
Asp
3.451AspAla: 3.451 ± 0.151
0.667AspCys: 0.667 ± 0.065
3.06AspAsp: 3.06 ± 0.225
2.44AspGlu: 2.44 ± 0.191
2.096AspPhe: 2.096 ± 0.144
3.228AspGly: 3.228 ± 0.215
0.762AspHis: 0.762 ± 0.078
4.178AspIle: 4.178 ± 0.199
2.285AspLys: 2.285 ± 0.112
3.302AspLeu: 3.302 ± 0.175
1.213AspMet: 1.213 ± 0.099
2.467AspAsn: 2.467 ± 0.156
2.184AspPro: 2.184 ± 0.14
0.863AspGln: 0.863 ± 0.093
1.914AspArg: 1.914 ± 0.123
2.736AspSer: 2.736 ± 0.155
3.457AspThr: 3.457 ± 0.194
3.976AspVal: 3.976 ± 0.175
0.627AspTrp: 0.627 ± 0.077
1.321AspTyr: 1.321 ± 0.12
0.0AspXaa: 0.0 ± 0.0
Glu
2.662GluAla: 2.662 ± 0.15
0.802GluCys: 0.802 ± 0.091
2.601GluAsp: 2.601 ± 0.229
2.77GluGlu: 2.77 ± 0.27
2.089GluPhe: 2.089 ± 0.128
2.002GluGly: 2.002 ± 0.136
1.287GluHis: 1.287 ± 0.1
2.979GluIle: 2.979 ± 0.168
3.221GluLys: 3.221 ± 0.236
3.477GluLeu: 3.477 ± 0.185
1.476GluMet: 1.476 ± 0.116
2.325GluAsn: 2.325 ± 0.134
1.833GluPro: 1.833 ± 0.169
1.341GluGln: 1.341 ± 0.103
2.359GluArg: 2.359 ± 0.158
2.75GluSer: 2.75 ± 0.152
2.979GluThr: 2.979 ± 0.168
2.804GluVal: 2.804 ± 0.169
0.499GluTrp: 0.499 ± 0.056
1.745GluTyr: 1.745 ± 0.124
0.0GluXaa: 0.0 ± 0.0
Phe
3.471PheAla: 3.471 ± 0.185
1.254PheCys: 1.254 ± 0.105
2.136PheAsp: 2.136 ± 0.141
2.21PheGlu: 2.21 ± 0.156
2.911PhePhe: 2.911 ± 0.174
3.39PheGly: 3.39 ± 0.257
1.159PheHis: 1.159 ± 0.097
2.723PheIle: 2.723 ± 0.142
2.285PheLys: 2.285 ± 0.128
5.129PheLeu: 5.129 ± 0.258
1.348PheMet: 1.348 ± 0.101
1.9PheAsn: 1.9 ± 0.129
3.242PhePro: 3.242 ± 0.268
1.429PheGln: 1.429 ± 0.114
1.88PheArg: 1.88 ± 0.116
4.468PheSer: 4.468 ± 0.234
3.006PheThr: 3.006 ± 0.188
3.693PheVal: 3.693 ± 0.162
0.815PheTrp: 0.815 ± 0.146
1.348PheTyr: 1.348 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
4.798GlyAla: 4.798 ± 0.442
0.97GlyCys: 0.97 ± 0.086
3.006GlyAsp: 3.006 ± 0.194
2.372GlyGlu: 2.372 ± 0.17
3.417GlyPhe: 3.417 ± 0.257
4.61GlyGly: 4.61 ± 0.339
1.28GlyHis: 1.28 ± 0.111
3.996GlyIle: 3.996 ± 0.19
4.327GlyLys: 4.327 ± 0.229
4.778GlyLeu: 4.778 ± 0.241
1.665GlyMet: 1.665 ± 0.143
5.331GlyAsn: 5.331 ± 0.835
2.029GlyPro: 2.029 ± 0.175
1.927GlyGln: 1.927 ± 0.188
3.242GlyArg: 3.242 ± 0.242
5.23GlySer: 5.23 ± 0.379
3.956GlyThr: 3.956 ± 0.255
4.872GlyVal: 4.872 ± 0.435
0.654GlyTrp: 0.654 ± 0.072
1.894GlyTyr: 1.894 ± 0.14
0.0GlyXaa: 0.0 ± 0.0
His
1.348HisAla: 1.348 ± 0.11
0.593HisCys: 0.593 ± 0.067
1.024HisAsp: 1.024 ± 0.091
0.937HisGlu: 0.937 ± 0.101
1.125HisPhe: 1.125 ± 0.094
1.577HisGly: 1.577 ± 0.132
0.984HisHis: 0.984 ± 0.106
1.617HisIle: 1.617 ± 0.129
1.274HisLys: 1.274 ± 0.106
2.291HisLeu: 2.291 ± 0.154
0.708HisMet: 0.708 ± 0.07
0.694HisAsn: 0.694 ± 0.08
1.072HisPro: 1.072 ± 0.098
0.829HisGln: 0.829 ± 0.063
1.51HisArg: 1.51 ± 0.144
1.631HisSer: 1.631 ± 0.127
1.2HisThr: 1.2 ± 0.119
1.59HisVal: 1.59 ± 0.128
0.384HisTrp: 0.384 ± 0.058
0.505HisTyr: 0.505 ± 0.057
0.0HisXaa: 0.0 ± 0.0
Ile
4.576IleAla: 4.576 ± 0.239
1.382IleCys: 1.382 ± 0.114
3.154IleAsp: 3.154 ± 0.157
2.467IleGlu: 2.467 ± 0.165
3.309IlePhe: 3.309 ± 0.186
4.111IleGly: 4.111 ± 0.398
1.382IleHis: 1.382 ± 0.113
4.199IleIle: 4.199 ± 0.261
3.255IleLys: 3.255 ± 0.168
5.58IleLeu: 5.58 ± 0.22
1.9IleMet: 1.9 ± 0.127
2.662IleAsn: 2.662 ± 0.197
3.424IlePro: 3.424 ± 0.185
1.887IleGln: 1.887 ± 0.138
3.012IleArg: 3.012 ± 0.143
5.425IleSer: 5.425 ± 0.202
4.111IleThr: 4.111 ± 0.222
4.758IleVal: 4.758 ± 0.251
0.842IleTrp: 0.842 ± 0.09
1.867IleTyr: 1.867 ± 0.12
0.0IleXaa: 0.0 ± 0.0
Lys
3.309LysAla: 3.309 ± 0.226
1.125LysCys: 1.125 ± 0.1
2.662LysAsp: 2.662 ± 0.149
3.329LysGlu: 3.329 ± 0.216
2.635LysPhe: 2.635 ± 0.138
2.534LysGly: 2.534 ± 0.163
1.422LysHis: 1.422 ± 0.104
3.424LysIle: 3.424 ± 0.178
5.519LysLys: 5.519 ± 0.291
4.583LysLeu: 4.583 ± 0.226
2.325LysMet: 2.325 ± 0.174
3.632LysAsn: 3.632 ± 0.176
3.626LysPro: 3.626 ± 0.383
1.813LysGln: 1.813 ± 0.137
2.804LysArg: 2.804 ± 0.161
4.279LysSer: 4.279 ± 0.261
3.889LysThr: 3.889 ± 0.198
3.43LysVal: 3.43 ± 0.248
0.573LysTrp: 0.573 ± 0.057
2.561LysTyr: 2.561 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
6.059LeuAla: 6.059 ± 0.251
1.961LeuCys: 1.961 ± 0.143
3.949LeuAsp: 3.949 ± 0.187
4.367LeuGlu: 4.367 ± 0.219
4.279LeuPhe: 4.279 ± 0.195
5.6LeuGly: 5.6 ± 0.268
2.231LeuHis: 2.231 ± 0.178
4.199LeuIle: 4.199 ± 0.203
4.327LeuLys: 4.327 ± 0.188
8.485LeuLeu: 8.485 ± 0.324
2.305LeuMet: 2.305 ± 0.129
3.531LeuAsn: 3.531 ± 0.141
5.445LeuPro: 5.445 ± 0.307
2.581LeuGln: 2.581 ± 0.138
4.664LeuArg: 4.664 ± 0.201
6.847LeuSer: 6.847 ± 0.277
4.893LeuThr: 4.893 ± 0.216
6.396LeuVal: 6.396 ± 0.237
1.294LeuTrp: 1.294 ± 0.109
2.595LeuTyr: 2.595 ± 0.118
0.0LeuXaa: 0.0 ± 0.0
Met
1.961MetAla: 1.961 ± 0.117
0.633MetCys: 0.633 ± 0.067
1.146MetAsp: 1.146 ± 0.105
1.186MetGlu: 1.186 ± 0.078
1.975MetPhe: 1.975 ± 0.142
1.55MetGly: 1.55 ± 0.175
0.458MetHis: 0.458 ± 0.058
1.53MetIle: 1.53 ± 0.103
1.685MetLys: 1.685 ± 0.122
3.167MetLeu: 3.167 ± 0.178
1.22MetMet: 1.22 ± 0.15
1.355MetAsn: 1.355 ± 0.093
1.678MetPro: 1.678 ± 0.12
0.809MetGln: 0.809 ± 0.079
1.981MetArg: 1.981 ± 0.169
2.945MetSer: 2.945 ± 0.175
2.184MetThr: 2.184 ± 0.143
1.907MetVal: 1.907 ± 0.14
0.371MetTrp: 0.371 ± 0.059
1.368MetTyr: 1.368 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.199
0.876AsnCys: 0.876 ± 0.084
2.177AsnAsp: 2.177 ± 0.151
1.698AsnGlu: 1.698 ± 0.105
2.446AsnPhe: 2.446 ± 0.156
3.296AsnGly: 3.296 ± 0.208
1.065AsnHis: 1.065 ± 0.089
4.347AsnIle: 4.347 ± 0.418
2.467AsnLys: 2.467 ± 0.156
3.835AsnLeu: 3.835 ± 0.176
1.307AsnMet: 1.307 ± 0.088
2.649AsnAsn: 2.649 ± 0.309
2.143AsnPro: 2.143 ± 0.117
1.193AsnGln: 1.193 ± 0.095
2.035AsnArg: 2.035 ± 0.149
3.531AsnSer: 3.531 ± 0.223
3.767AsnThr: 3.767 ± 0.346
5.695AsnVal: 5.695 ± 0.79
0.532AsnTrp: 0.532 ± 0.059
1.287AsnTyr: 1.287 ± 0.102
0.0AsnXaa: 0.0 ± 0.0
Pro
4.421ProAla: 4.421 ± 0.463
0.984ProCys: 0.984 ± 0.093
2.21ProAsp: 2.21 ± 0.143
2.891ProGlu: 2.891 ± 0.245
1.988ProPhe: 1.988 ± 0.156
3.592ProGly: 3.592 ± 0.271
1.206ProHis: 1.206 ± 0.107
2.595ProIle: 2.595 ± 0.16
3.781ProLys: 3.781 ± 0.425
4.023ProLeu: 4.023 ± 0.207
2.062ProMet: 2.062 ± 0.189
1.948ProAsn: 1.948 ± 0.129
2.884ProPro: 2.884 ± 0.232
1.415ProGln: 1.415 ± 0.128
2.959ProArg: 2.959 ± 0.188
4.603ProSer: 4.603 ± 0.244
3.922ProThr: 3.922 ± 0.369
4.529ProVal: 4.529 ± 0.311
0.553ProTrp: 0.553 ± 0.076
1.314ProTyr: 1.314 ± 0.114
0.0ProXaa: 0.0 ± 0.0
Gln
1.469GlnAla: 1.469 ± 0.104
0.546GlnCys: 0.546 ± 0.071
1.038GlnAsp: 1.038 ± 0.084
1.462GlnGlu: 1.462 ± 0.105
1.267GlnPhe: 1.267 ± 0.096
1.442GlnGly: 1.442 ± 0.119
0.836GlnHis: 0.836 ± 0.077
1.604GlnIle: 1.604 ± 0.115
2.089GlnLys: 2.089 ± 0.176
2.608GlnLeu: 2.608 ± 0.139
1.065GlnMet: 1.065 ± 0.1
1.651GlnAsn: 1.651 ± 0.139
1.159GlnPro: 1.159 ± 0.109
1.307GlnGln: 1.307 ± 0.147
1.833GlnArg: 1.833 ± 0.139
1.995GlnSer: 1.995 ± 0.148
1.874GlnThr: 1.874 ± 0.128
1.954GlnVal: 1.954 ± 0.14
0.431GlnTrp: 0.431 ± 0.059
1.078GlnTyr: 1.078 ± 0.086
0.0GlnXaa: 0.0 ± 0.0
Arg
2.999ArgAla: 2.999 ± 0.151
1.132ArgCys: 1.132 ± 0.15
2.264ArgAsp: 2.264 ± 0.119
2.136ArgGlu: 2.136 ± 0.13
2.042ArgPhe: 2.042 ± 0.147
3.201ArgGly: 3.201 ± 0.205
1.341ArgHis: 1.341 ± 0.114
3.161ArgIle: 3.161 ± 0.17
3.424ArgLys: 3.424 ± 0.257
4.212ArgLeu: 4.212 ± 0.215
1.921ArgMet: 1.921 ± 0.162
2.48ArgAsn: 2.48 ± 0.141
2.817ArgPro: 2.817 ± 0.229
1.698ArgGln: 1.698 ± 0.122
3.457ArgArg: 3.457 ± 0.246
4.239ArgSer: 4.239 ± 0.241
3.316ArgThr: 3.316 ± 0.175
3.976ArgVal: 3.976 ± 0.183
0.708ArgTrp: 0.708 ± 0.074
1.577ArgTyr: 1.577 ± 0.101
0.0ArgXaa: 0.0 ± 0.0
Ser
5.23SerAla: 5.23 ± 0.258
1.719SerCys: 1.719 ± 0.121
3.019SerAsp: 3.019 ± 0.143
2.965SerGlu: 2.965 ± 0.136
4.306SerPhe: 4.306 ± 0.204
5.937SerGly: 5.937 ± 0.393
1.692SerHis: 1.692 ± 0.133
5.29SerIle: 5.29 ± 0.184
4.394SerLys: 4.394 ± 0.216
7.036SerLeu: 7.036 ± 0.318
2.635SerMet: 2.635 ± 0.151
3.68SerAsn: 3.68 ± 0.267
4.529SerPro: 4.529 ± 0.309
2.015SerGln: 2.015 ± 0.159
4.738SerArg: 4.738 ± 0.217
8.613SerSer: 8.613 ± 0.499
5.432SerThr: 5.432 ± 0.258
5.317SerVal: 5.317 ± 0.214
0.991SerTrp: 0.991 ± 0.101
2.184SerTyr: 2.184 ± 0.164
0.0SerXaa: 0.0 ± 0.0
Thr
3.902ThrAla: 3.902 ± 0.217
1.166ThrCys: 1.166 ± 0.094
2.46ThrAsp: 2.46 ± 0.155
2.372ThrGlu: 2.372 ± 0.145
3.579ThrPhe: 3.579 ± 0.254
4.798ThrGly: 4.798 ± 0.353
1.24ThrHis: 1.24 ± 0.101
4.104ThrIle: 4.104 ± 0.225
3.713ThrLys: 3.713 ± 0.201
5.803ThrLeu: 5.803 ± 0.22
1.84ThrMet: 1.84 ± 0.129
2.938ThrAsn: 2.938 ± 0.186
4.536ThrPro: 4.536 ± 0.389
1.745ThrGln: 1.745 ± 0.128
3.329ThrArg: 3.329 ± 0.156
5.472ThrSer: 5.472 ± 0.241
4.306ThrThr: 4.306 ± 0.243
3.808ThrVal: 3.808 ± 0.193
0.775ThrTrp: 0.775 ± 0.072
2.157ThrTyr: 2.157 ± 0.121
0.0ThrXaa: 0.0 ± 0.0
Val
4.576ValAla: 4.576 ± 0.186
1.409ValCys: 1.409 ± 0.118
3.781ValAsp: 3.781 ± 0.167
3.039ValGlu: 3.039 ± 0.176
3.787ValPhe: 3.787 ± 0.179
4.832ValGly: 4.832 ± 0.478
1.496ValHis: 1.496 ± 0.132
4.724ValIle: 4.724 ± 0.196
4.097ValLys: 4.097 ± 0.209
6.827ValLeu: 6.827 ± 0.253
2.231ValMet: 2.231 ± 0.131
3.424ValAsn: 3.424 ± 0.2
3.895ValPro: 3.895 ± 0.251
1.914ValGln: 1.914 ± 0.135
3.383ValArg: 3.383 ± 0.196
6.423ValSer: 6.423 ± 0.366
4.017ValThr: 4.017 ± 0.258
5.749ValVal: 5.749 ± 0.31
0.809ValTrp: 0.809 ± 0.074
2.339ValTyr: 2.339 ± 0.152
0.0ValXaa: 0.0 ± 0.0
Trp
0.829TrpAla: 0.829 ± 0.091
0.323TrpCys: 0.323 ± 0.047
0.58TrpAsp: 0.58 ± 0.064
0.559TrpGlu: 0.559 ± 0.059
0.728TrpPhe: 0.728 ± 0.071
0.728TrpGly: 0.728 ± 0.097
0.263TrpHis: 0.263 ± 0.042
0.62TrpIle: 0.62 ± 0.065
0.923TrpLys: 0.923 ± 0.078
0.842TrpLeu: 0.842 ± 0.082
0.418TrpMet: 0.418 ± 0.055
0.768TrpAsn: 0.768 ± 0.085
0.404TrpPro: 0.404 ± 0.057
0.431TrpGln: 0.431 ± 0.057
0.775TrpArg: 0.775 ± 0.084
1.099TrpSer: 1.099 ± 0.091
0.755TrpThr: 0.755 ± 0.086
0.647TrpVal: 0.647 ± 0.065
0.195TrpTrp: 0.195 ± 0.037
0.512TrpTyr: 0.512 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.359TyrAla: 2.359 ± 0.176
0.485TyrCys: 0.485 ± 0.059
2.082TyrAsp: 2.082 ± 0.134
1.442TyrGlu: 1.442 ± 0.098
1.665TyrPhe: 1.665 ± 0.091
1.826TyrGly: 1.826 ± 0.14
0.667TyrHis: 0.667 ± 0.068
2.318TyrIle: 2.318 ± 0.129
1.665TyrLys: 1.665 ± 0.118
2.608TyrLeu: 2.608 ± 0.135
0.836TyrMet: 0.836 ± 0.071
1.779TyrAsn: 1.779 ± 0.152
1.146TyrPro: 1.146 ± 0.126
0.923TyrGln: 0.923 ± 0.077
1.584TyrArg: 1.584 ± 0.136
2.13TyrSer: 2.13 ± 0.132
2.264TyrThr: 2.264 ± 0.135
2.217TyrVal: 2.217 ± 0.142
0.256TyrTrp: 0.256 ± 0.063
0.863TyrTyr: 0.863 ± 0.083
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 841 proteins (148385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski