Amino acid dipepetide frequency for Bacillus phage Chotacabras

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.168AlaAla: 3.168 ± 0.491
0.379AlaCys: 0.379 ± 0.091
3.706AlaAsp: 3.706 ± 0.316
4.164AlaGlu: 4.164 ± 0.32
2.331AlaPhe: 2.331 ± 0.228
3.746AlaGly: 3.746 ± 0.337
0.797AlaHis: 0.797 ± 0.124
3.925AlaIle: 3.925 ± 0.32
4.742AlaLys: 4.742 ± 0.357
5.121AlaLeu: 5.121 ± 0.381
1.913AlaMet: 1.913 ± 0.23
2.869AlaAsn: 2.869 ± 0.259
2.212AlaPro: 2.212 ± 0.276
2.032AlaGln: 2.032 ± 0.257
2.849AlaArg: 2.849 ± 0.262
3.288AlaSer: 3.288 ± 0.32
4.005AlaThr: 4.005 ± 0.47
3.587AlaVal: 3.587 ± 0.314
0.638AlaTrp: 0.638 ± 0.104
2.491AlaTyr: 2.491 ± 0.247
0.0AlaXaa: 0.0 ± 0.0
Cys
0.379CysAla: 0.379 ± 0.074
0.12CysCys: 0.12 ± 0.062
0.578CysAsp: 0.578 ± 0.132
0.797CysGlu: 0.797 ± 0.16
0.279CysPhe: 0.279 ± 0.078
0.697CysGly: 0.697 ± 0.149
0.239CysHis: 0.239 ± 0.073
0.598CysIle: 0.598 ± 0.116
0.737CysLys: 0.737 ± 0.133
0.578CysLeu: 0.578 ± 0.112
0.319CysMet: 0.319 ± 0.101
0.399CysAsn: 0.399 ± 0.095
0.319CysPro: 0.319 ± 0.084
0.159CysGln: 0.159 ± 0.054
0.299CysArg: 0.299 ± 0.082
0.458CysSer: 0.458 ± 0.098
0.478CysThr: 0.478 ± 0.097
0.458CysVal: 0.458 ± 0.077
0.12CysTrp: 0.12 ± 0.054
0.598CysTyr: 0.598 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
3.726AspAla: 3.726 ± 0.304
0.558AspCys: 0.558 ± 0.116
3.447AspAsp: 3.447 ± 0.317
5.22AspGlu: 5.22 ± 0.399
2.909AspPhe: 2.909 ± 0.253
3.945AspGly: 3.945 ± 0.4
0.518AspHis: 0.518 ± 0.089
5.579AspIle: 5.579 ± 0.354
5.918AspLys: 5.918 ± 0.381
5.101AspLeu: 5.101 ± 0.303
2.052AspMet: 2.052 ± 0.232
3.666AspAsn: 3.666 ± 0.299
1.474AspPro: 1.474 ± 0.259
0.857AspGln: 0.857 ± 0.143
2.531AspArg: 2.531 ± 0.208
3.527AspSer: 3.527 ± 0.222
3.746AspThr: 3.746 ± 0.363
4.224AspVal: 4.224 ± 0.277
1.056AspTrp: 1.056 ± 0.129
3.826AspTyr: 3.826 ± 0.227
0.0AspXaa: 0.0 ± 0.0
Glu
4.722GluAla: 4.722 ± 0.34
0.658GluCys: 0.658 ± 0.138
5.579GluAsp: 5.579 ± 0.446
9.823GluGlu: 9.823 ± 0.951
3.248GluPhe: 3.248 ± 0.252
4.842GluGly: 4.842 ± 0.277
1.893GluHis: 1.893 ± 0.242
5.161GluIle: 5.161 ± 0.365
6.316GluLys: 6.316 ± 0.48
8.209GluLeu: 8.209 ± 0.52
2.75GluMet: 2.75 ± 0.287
3.905GluAsn: 3.905 ± 0.267
1.833GluPro: 1.833 ± 0.219
3.168GluGln: 3.168 ± 0.335
3.646GluArg: 3.646 ± 0.272
3.128GluSer: 3.128 ± 0.282
3.806GluThr: 3.806 ± 0.253
7.054GluVal: 7.054 ± 0.444
1.415GluTrp: 1.415 ± 0.176
4.045GluTyr: 4.045 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
1.953PheAla: 1.953 ± 0.196
0.399PheCys: 0.399 ± 0.085
2.809PheAsp: 2.809 ± 0.218
3.009PheGlu: 3.009 ± 0.223
1.176PhePhe: 1.176 ± 0.179
2.052PheGly: 2.052 ± 0.207
0.717PheHis: 0.717 ± 0.124
2.969PheIle: 2.969 ± 0.258
3.288PheLys: 3.288 ± 0.241
3.128PheLeu: 3.128 ± 0.287
1.076PheMet: 1.076 ± 0.142
2.491PheAsn: 2.491 ± 0.211
1.295PhePro: 1.295 ± 0.149
1.016PheGln: 1.016 ± 0.152
1.574PheArg: 1.574 ± 0.161
2.75PheSer: 2.75 ± 0.311
2.949PheThr: 2.949 ± 0.28
2.67PheVal: 2.67 ± 0.27
0.299PheTrp: 0.299 ± 0.074
1.893PheTyr: 1.893 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
3.567GlyAla: 3.567 ± 0.405
0.677GlyCys: 0.677 ± 0.143
3.746GlyAsp: 3.746 ± 0.341
4.682GlyGlu: 4.682 ± 0.325
2.929GlyPhe: 2.929 ± 0.243
5.32GlyGly: 5.32 ± 0.783
1.335GlyHis: 1.335 ± 0.173
4.324GlyIle: 4.324 ± 0.357
5.559GlyLys: 5.559 ± 0.376
4.583GlyLeu: 4.583 ± 0.321
1.793GlyMet: 1.793 ± 0.199
3.308GlyAsn: 3.308 ± 0.329
0.0GlyPro: 0.0 ± 0.0
2.072GlyGln: 2.072 ± 0.269
2.351GlyArg: 2.351 ± 0.204
3.866GlySer: 3.866 ± 0.408
3.945GlyThr: 3.945 ± 0.433
5.061GlyVal: 5.061 ± 0.306
0.956GlyTrp: 0.956 ± 0.132
3.288GlyTyr: 3.288 ± 0.282
0.0GlyXaa: 0.0 ± 0.0
His
0.936HisAla: 0.936 ± 0.142
0.179HisCys: 0.179 ± 0.068
1.235HisAsp: 1.235 ± 0.13
1.275HisGlu: 1.275 ± 0.168
0.677HisPhe: 0.677 ± 0.12
1.016HisGly: 1.016 ± 0.129
0.319HisHis: 0.319 ± 0.086
1.315HisIle: 1.315 ± 0.192
1.295HisLys: 1.295 ± 0.176
1.375HisLeu: 1.375 ± 0.185
0.498HisMet: 0.498 ± 0.097
1.036HisAsn: 1.036 ± 0.133
0.638HisPro: 0.638 ± 0.12
0.558HisGln: 0.558 ± 0.128
0.956HisArg: 0.956 ± 0.131
1.275HisSer: 1.275 ± 0.183
1.036HisThr: 1.036 ± 0.135
1.574HisVal: 1.574 ± 0.208
0.159HisTrp: 0.159 ± 0.063
0.757HisTyr: 0.757 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
4.204IleAla: 4.204 ± 0.311
0.478IleCys: 0.478 ± 0.104
5.081IleAsp: 5.081 ± 0.337
6.217IleGlu: 6.217 ± 0.419
2.132IlePhe: 2.132 ± 0.218
3.607IleGly: 3.607 ± 0.277
0.936IleHis: 0.936 ± 0.138
3.925IleIle: 3.925 ± 0.293
6.316IleLys: 6.316 ± 0.326
4.742IleLeu: 4.742 ± 0.293
1.773IleMet: 1.773 ± 0.214
3.866IleAsn: 3.866 ± 0.32
2.212IlePro: 2.212 ± 0.234
2.152IleGln: 2.152 ± 0.208
2.471IleArg: 2.471 ± 0.226
3.866IleSer: 3.866 ± 0.246
4.523IleThr: 4.523 ± 0.391
4.244IleVal: 4.244 ± 0.299
0.578IleTrp: 0.578 ± 0.101
2.69IleTyr: 2.69 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
5.101LysAla: 5.101 ± 0.356
0.658LysCys: 0.658 ± 0.158
5.201LysAsp: 5.201 ± 0.325
8.309LysGlu: 8.309 ± 0.697
3.029LysPhe: 3.029 ± 0.266
5.559LysGly: 5.559 ± 0.34
1.514LysHis: 1.514 ± 0.174
4.423LysIle: 4.423 ± 0.27
6.834LysLys: 6.834 ± 0.472
6.316LysLeu: 6.316 ± 0.373
2.63LysMet: 2.63 ± 0.227
4.184LysAsn: 4.184 ± 0.304
2.272LysPro: 2.272 ± 0.251
3.407LysGln: 3.407 ± 0.315
3.547LysArg: 3.547 ± 0.283
3.885LysSer: 3.885 ± 0.286
4.085LysThr: 4.085 ± 0.252
5.978LysVal: 5.978 ± 0.312
0.976LysTrp: 0.976 ± 0.133
3.766LysTyr: 3.766 ± 0.3
0.0LysXaa: 0.0 ± 0.0
Leu
4.842LeuAla: 4.842 ± 0.358
0.558LeuCys: 0.558 ± 0.117
5.619LeuAsp: 5.619 ± 0.367
7.193LeuGlu: 7.193 ± 0.487
2.869LeuPhe: 2.869 ± 0.26
4.742LeuGly: 4.742 ± 0.305
1.813LeuHis: 1.813 ± 0.171
4.742LeuIle: 4.742 ± 0.346
5.878LeuLys: 5.878 ± 0.406
5.858LeuLeu: 5.858 ± 0.427
2.192LeuMet: 2.192 ± 0.229
4.184LeuAsn: 4.184 ± 0.246
2.61LeuPro: 2.61 ± 0.233
2.491LeuGln: 2.491 ± 0.213
3.726LeuArg: 3.726 ± 0.334
4.882LeuSer: 4.882 ± 0.296
5.181LeuThr: 5.181 ± 0.327
5.24LeuVal: 5.24 ± 0.369
0.857LeuTrp: 0.857 ± 0.142
3.607LeuTyr: 3.607 ± 0.234
0.0LeuXaa: 0.0 ± 0.0
Met
1.973MetAla: 1.973 ± 0.2
0.179MetCys: 0.179 ± 0.061
1.793MetAsp: 1.793 ± 0.229
2.351MetGlu: 2.351 ± 0.235
1.215MetPhe: 1.215 ± 0.165
1.255MetGly: 1.255 ± 0.205
0.438MetHis: 0.438 ± 0.092
1.674MetIle: 1.674 ± 0.197
2.77MetLys: 2.77 ± 0.207
2.351MetLeu: 2.351 ± 0.244
0.697MetMet: 0.697 ± 0.116
1.873MetAsn: 1.873 ± 0.197
0.618MetPro: 0.618 ± 0.113
0.917MetGln: 0.917 ± 0.139
1.355MetArg: 1.355 ± 0.153
2.032MetSer: 2.032 ± 0.193
2.272MetThr: 2.272 ± 0.199
1.734MetVal: 1.734 ± 0.202
0.458MetTrp: 0.458 ± 0.092
1.275MetTyr: 1.275 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.188AsnAla: 3.188 ± 0.273
0.418AsnCys: 0.418 ± 0.16
3.188AsnAsp: 3.188 ± 0.221
3.666AsnGlu: 3.666 ± 0.28
1.813AsnPhe: 1.813 ± 0.212
4.324AsnGly: 4.324 ± 0.428
0.956AsnHis: 0.956 ± 0.13
3.487AsnIle: 3.487 ± 0.283
4.663AsnLys: 4.663 ± 0.319
4.025AsnLeu: 4.025 ± 0.271
2.112AsnMet: 2.112 ± 0.194
3.347AsnAsn: 3.347 ± 0.305
2.272AsnPro: 2.272 ± 0.282
1.753AsnGln: 1.753 ± 0.211
2.79AsnArg: 2.79 ± 0.237
2.57AsnSer: 2.57 ± 0.297
3.228AsnThr: 3.228 ± 0.264
3.686AsnVal: 3.686 ± 0.262
0.658AsnTrp: 0.658 ± 0.112
2.829AsnTyr: 2.829 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
1.953ProAla: 1.953 ± 0.338
0.199ProCys: 0.199 ± 0.059
1.634ProAsp: 1.634 ± 0.165
2.65ProGlu: 2.65 ± 0.24
1.355ProPhe: 1.355 ± 0.171
0.737ProGly: 0.737 ± 0.14
0.598ProHis: 0.598 ± 0.109
1.714ProIle: 1.714 ± 0.215
2.092ProLys: 2.092 ± 0.167
2.291ProLeu: 2.291 ± 0.213
0.857ProMet: 0.857 ± 0.162
1.534ProAsn: 1.534 ± 0.185
0.897ProPro: 0.897 ± 0.172
1.076ProGln: 1.076 ± 0.169
1.196ProArg: 1.196 ± 0.136
1.833ProSer: 1.833 ± 0.206
2.052ProThr: 2.052 ± 0.261
1.953ProVal: 1.953 ± 0.223
0.259ProTrp: 0.259 ± 0.071
1.594ProTyr: 1.594 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
2.511GlnAla: 2.511 ± 0.261
0.339GlnCys: 0.339 ± 0.082
1.833GlnAsp: 1.833 ± 0.233
2.71GlnGlu: 2.71 ± 0.21
1.136GlnPhe: 1.136 ± 0.175
2.252GlnGly: 2.252 ± 0.242
0.737GlnHis: 0.737 ± 0.132
1.993GlnIle: 1.993 ± 0.189
2.471GlnLys: 2.471 ± 0.232
2.531GlnLeu: 2.531 ± 0.208
0.956GlnMet: 0.956 ± 0.148
1.674GlnAsn: 1.674 ± 0.217
1.156GlnPro: 1.156 ± 0.159
1.534GlnGln: 1.534 ± 0.208
1.215GlnArg: 1.215 ± 0.148
2.012GlnSer: 2.012 ± 0.235
1.893GlnThr: 1.893 ± 0.194
2.172GlnVal: 2.172 ± 0.219
0.458GlnTrp: 0.458 ± 0.093
1.275GlnTyr: 1.275 ± 0.125
0.0GlnXaa: 0.0 ± 0.0
Arg
2.112ArgAla: 2.112 ± 0.22
0.339ArgCys: 0.339 ± 0.096
2.65ArgAsp: 2.65 ± 0.274
4.005ArgGlu: 4.005 ± 0.29
2.072ArgPhe: 2.072 ± 0.188
3.029ArgGly: 3.029 ± 0.23
0.697ArgHis: 0.697 ± 0.109
2.869ArgIle: 2.869 ± 0.276
3.108ArgLys: 3.108 ± 0.29
3.866ArgLeu: 3.866 ± 0.277
1.196ArgMet: 1.196 ± 0.154
2.232ArgAsn: 2.232 ± 0.199
1.036ArgPro: 1.036 ± 0.136
1.773ArgGln: 1.773 ± 0.205
1.494ArgArg: 1.494 ± 0.178
1.694ArgSer: 1.694 ± 0.182
2.351ArgThr: 2.351 ± 0.225
2.71ArgVal: 2.71 ± 0.257
0.538ArgTrp: 0.538 ± 0.11
2.192ArgTyr: 2.192 ± 0.228
0.0ArgXaa: 0.0 ± 0.0
Ser
3.168SerAla: 3.168 ± 0.35
0.458SerCys: 0.458 ± 0.106
3.527SerAsp: 3.527 ± 0.27
3.686SerGlu: 3.686 ± 0.365
2.67SerPhe: 2.67 ± 0.297
4.105SerGly: 4.105 ± 0.385
0.917SerHis: 0.917 ± 0.167
3.965SerIle: 3.965 ± 0.271
4.264SerLys: 4.264 ± 0.302
4.463SerLeu: 4.463 ± 0.296
1.574SerMet: 1.574 ± 0.156
3.049SerAsn: 3.049 ± 0.295
1.455SerPro: 1.455 ± 0.188
1.455SerGln: 1.455 ± 0.161
2.192SerArg: 2.192 ± 0.225
3.288SerSer: 3.288 ± 0.354
3.746SerThr: 3.746 ± 0.332
3.706SerVal: 3.706 ± 0.319
0.857SerTrp: 0.857 ± 0.145
2.77SerTyr: 2.77 ± 0.227
0.0SerXaa: 0.0 ± 0.0
Thr
3.367ThrAla: 3.367 ± 0.281
0.618ThrCys: 0.618 ± 0.119
3.706ThrAsp: 3.706 ± 0.354
4.025ThrGlu: 4.025 ± 0.263
2.79ThrPhe: 2.79 ± 0.233
4.344ThrGly: 4.344 ± 0.354
1.136ThrHis: 1.136 ± 0.148
3.806ThrIle: 3.806 ± 0.355
4.663ThrLys: 4.663 ± 0.277
5.001ThrLeu: 5.001 ± 0.331
1.455ThrMet: 1.455 ± 0.208
3.607ThrAsn: 3.607 ± 0.334
2.451ThrPro: 2.451 ± 0.286
1.933ThrGln: 1.933 ± 0.171
2.59ThrArg: 2.59 ± 0.206
3.726ThrSer: 3.726 ± 0.369
3.646ThrThr: 3.646 ± 0.324
5.161ThrVal: 5.161 ± 0.443
0.757ThrTrp: 0.757 ± 0.147
3.427ThrTyr: 3.427 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
3.885ValAla: 3.885 ± 0.297
0.598ValCys: 0.598 ± 0.119
4.563ValAsp: 4.563 ± 0.311
6.217ValGlu: 6.217 ± 0.422
2.869ValPhe: 2.869 ± 0.251
4.344ValGly: 4.344 ± 0.272
1.355ValHis: 1.355 ± 0.149
4.423ValIle: 4.423 ± 0.301
5.46ValLys: 5.46 ± 0.39
4.942ValLeu: 4.942 ± 0.352
1.654ValMet: 1.654 ± 0.166
3.945ValAsn: 3.945 ± 0.305
2.252ValPro: 2.252 ± 0.217
2.272ValGln: 2.272 ± 0.216
2.73ValArg: 2.73 ± 0.199
4.144ValSer: 4.144 ± 0.315
5.22ValThr: 5.22 ± 0.372
5.32ValVal: 5.32 ± 0.34
0.697ValTrp: 0.697 ± 0.131
3.487ValTyr: 3.487 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
0.538TrpAla: 0.538 ± 0.094
0.219TrpCys: 0.219 ± 0.058
0.797TrpAsp: 0.797 ± 0.133
1.116TrpGlu: 1.116 ± 0.136
0.498TrpPhe: 0.498 ± 0.103
0.757TrpGly: 0.757 ± 0.127
0.438TrpHis: 0.438 ± 0.09
0.956TrpIle: 0.956 ± 0.147
0.877TrpLys: 0.877 ± 0.145
1.016TrpLeu: 1.016 ± 0.138
0.399TrpMet: 0.399 ± 0.098
0.777TrpAsn: 0.777 ± 0.158
0.0TrpPro: 0.0 ± 0.0
0.359TrpGln: 0.359 ± 0.099
0.498TrpArg: 0.498 ± 0.088
0.658TrpSer: 0.658 ± 0.112
0.677TrpThr: 0.677 ± 0.14
1.076TrpVal: 1.076 ± 0.157
0.179TrpTrp: 0.179 ± 0.056
0.677TrpTyr: 0.677 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.67TyrAla: 2.67 ± 0.182
0.578TyrCys: 0.578 ± 0.119
3.188TyrAsp: 3.188 ± 0.248
4.085TyrGlu: 4.085 ± 0.285
1.514TyrPhe: 1.514 ± 0.173
2.69TyrGly: 2.69 ± 0.239
0.757TyrHis: 0.757 ± 0.122
4.164TyrIle: 4.164 ± 0.325
4.423TyrLys: 4.423 ± 0.349
3.587TyrLeu: 3.587 ± 0.276
1.315TyrMet: 1.315 ± 0.157
3.049TyrAsn: 3.049 ± 0.189
1.474TyrPro: 1.474 ± 0.168
1.873TyrGln: 1.873 ± 0.215
1.953TyrArg: 1.953 ± 0.197
2.431TyrSer: 2.431 ± 0.219
3.367TyrThr: 3.367 ± 0.264
2.77TyrVal: 2.77 ± 0.288
0.598TyrTrp: 0.598 ± 0.108
2.192TyrTyr: 2.192 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 284 proteins (50188 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski