Schleiferia thermophila str. Yellowstone

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Schleiferiaceae; Schleiferia; Schleiferia thermophila

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085L0G3|A0A085L0G3_9FLAO Uncharacterized protein OS=Schleiferia thermophila str. Yellowstone OX=1453500 GN=AT05_10210 PE=4 SV=1
MM1 pKa = 7.1NHH3 pKa = 5.37QAALCSCTSDD13 pKa = 3.04AFVVLNFSLMLPPQKK28 pKa = 10.0EE29 pKa = 3.8AAFFIPVQDD38 pKa = 4.07FADD41 pKa = 4.04LEE43 pKa = 4.63SS44 pKa = 3.58

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085L5W9|A0A085L5W9_9FLAO Uncharacterized protein OS=Schleiferia thermophila str. Yellowstone OX=1453500 GN=AT05_02235 PE=4 SV=1
MM1 pKa = 7.25IAAGGRR7 pKa = 11.84STAPIRR13 pKa = 11.84LAVRR17 pKa = 11.84AWHH20 pKa = 7.04IGLRR24 pKa = 11.84WLAPLRR30 pKa = 11.84LLARR34 pKa = 11.84EE35 pKa = 4.02PHH37 pKa = 6.14SPPAAAHH44 pKa = 5.35IRR46 pKa = 11.84LRR48 pKa = 11.84PGACTMRR55 pKa = 11.84QGFNDD60 pKa = 3.01IDD62 pKa = 3.96KK63 pKa = 9.94PPKK66 pKa = 9.95SVLFSTLPAIWLSPADD82 pKa = 3.52DD83 pKa = 4.37CFFNALVDD91 pKa = 5.15RR92 pKa = 11.84IQSADD97 pKa = 3.71KK98 pKa = 8.68TWKK101 pKa = 10.44HH102 pKa = 5.97SGQPSLICTAHH113 pKa = 7.55LIQFCSSHH121 pKa = 6.73RR122 pKa = 11.84NLVSNNPLSIQPKK135 pKa = 7.58PHH137 pKa = 6.5IFSSRR142 pKa = 11.84HH143 pKa = 4.21GQNFSTPVEE152 pKa = 3.85ALKK155 pKa = 10.59IHH157 pKa = 5.97ITSNLIYY164 pKa = 10.23LPYY167 pKa = 9.77MPAAKK172 pKa = 9.83KK173 pKa = 9.06STVIFSSITRR183 pKa = 11.84SYY185 pKa = 10.78NN186 pKa = 2.96

Molecular weight: 20.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2301

0

2301

783700

29

2724

340.6

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.731 ± 0.051

0.803 ± 0.016

4.983 ± 0.037

6.083 ± 0.067

5.243 ± 0.041

6.597 ± 0.049

2.184 ± 0.027

7.712 ± 0.048

6.199 ± 0.071

9.861 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.023

5.19 ± 0.062

4.242 ± 0.037

3.691 ± 0.03

4.782 ± 0.04

6.399 ± 0.05

5.549 ± 0.063

6.246 ± 0.039

1.211 ± 0.021

4.103 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski