Tetrasphaera japonica T1-X7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Tetrasphaera; Tetrasphaera japonica

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077M2R6|A0A077M2R6_9MICO Putative nitrate reductase delta subunit (NarJ) OS=Tetrasphaera japonica T1-X7 OX=1194083 GN=BN12_300022 PE=4 SV=1
MM1 pKa = 7.81PGGGFGGAGGGLPGGRR17 pKa = 11.84TQGGTGTSNGTGSQGGTSNGTGSSDD42 pKa = 3.13GTGAQGGTSNGTGQTQQGQGTTGGATTGGPGGGMGGLLNASTPNTEE88 pKa = 3.81VVAALTANADD98 pKa = 3.57DD99 pKa = 4.33YY100 pKa = 10.58TWVAAAIGSQNAAGLQLASQEE121 pKa = 4.03PVMAIGGFNGSDD133 pKa = 3.71PSPTLAQFEE142 pKa = 5.01AYY144 pKa = 9.84VKK146 pKa = 10.42AGKK149 pKa = 8.87IHH151 pKa = 6.34YY152 pKa = 9.16FLASSIGEE160 pKa = 4.05GGGNGTASQITQWVEE175 pKa = 3.53DD176 pKa = 4.12NYY178 pKa = 10.12TQVTIGGTTFYY189 pKa = 11.31DD190 pKa = 3.77LTQPLSSSSSSSSTGSSTTGG210 pKa = 2.89

Molecular weight: 19.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077LT34|A0A077LT34_9MICO Transcriptional regulator GntR family OS=Tetrasphaera japonica T1-X7 OX=1194083 GN=BN12_1060008 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4595

0

4595

1426106

11

2276

310.4

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.227 ± 0.05

0.776 ± 0.01

6.269 ± 0.028

5.245 ± 0.033

2.666 ± 0.023

9.349 ± 0.028

2.301 ± 0.017

3.508 ± 0.026

1.656 ± 0.026

10.102 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.815 ± 0.015

1.617 ± 0.018

5.881 ± 0.032

2.546 ± 0.018

8.14 ± 0.043

5.511 ± 0.029

6.387 ± 0.031

9.498 ± 0.036

1.546 ± 0.015

1.961 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski