Amino acid dipepetide frequency for Naegleria fowleri (Brain eating amoeba)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.672AlaAla: 2.672 ± 0.024
0.862AlaCys: 0.862 ± 0.013
1.826AlaAsp: 1.826 ± 0.02
2.455AlaGlu: 2.455 ± 0.022
2.215AlaPhe: 2.215 ± 0.023
1.836AlaGly: 1.836 ± 0.02
1.023AlaHis: 1.023 ± 0.011
3.167AlaIle: 3.167 ± 0.023
3.184AlaLys: 3.184 ± 0.025
4.882AlaLeu: 4.882 ± 0.029
1.14AlaMet: 1.14 ± 0.014
2.442AlaAsn: 2.442 ± 0.022
1.626AlaPro: 1.626 ± 0.015
1.915AlaGln: 1.915 ± 0.022
1.743AlaArg: 1.743 ± 0.019
4.78AlaSer: 4.78 ± 0.032
3.195AlaThr: 3.195 ± 0.023
2.633AlaVal: 2.633 ± 0.02
0.367AlaTrp: 0.367 ± 0.012
1.333AlaTyr: 1.333 ± 0.014
0.0AlaXaa: 0.0 ± 0.0
Cys
0.705CysAla: 0.705 ± 0.011
0.473CysCys: 0.473 ± 0.01
0.828CysAsp: 0.828 ± 0.012
0.924CysGlu: 0.924 ± 0.014
0.89CysPhe: 0.89 ± 0.014
0.936CysGly: 0.936 ± 0.015
0.428CysHis: 0.428 ± 0.009
1.06CysIle: 1.06 ± 0.013
1.065CysLys: 1.065 ± 0.012
1.503CysLeu: 1.503 ± 0.016
0.397CysMet: 0.397 ± 0.008
0.869CysAsn: 0.869 ± 0.017
0.597CysPro: 0.597 ± 0.012
0.573CysGln: 0.573 ± 0.009
0.58CysArg: 0.58 ± 0.009
1.493CysSer: 1.493 ± 0.023
0.832CysThr: 0.832 ± 0.011
1.036CysVal: 1.036 ± 0.013
0.153CysTrp: 0.153 ± 0.005
0.577CysTyr: 0.577 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
2.287AspAla: 2.287 ± 0.019
0.731AspCys: 0.731 ± 0.011
3.994AspAsp: 3.994 ± 0.035
4.626AspGlu: 4.626 ± 0.03
2.512AspPhe: 2.512 ± 0.02
2.289AspGly: 2.289 ± 0.022
1.621AspHis: 1.621 ± 0.016
3.426AspIle: 3.426 ± 0.026
3.004AspLys: 3.004 ± 0.023
4.809AspLeu: 4.809 ± 0.028
1.234AspMet: 1.234 ± 0.012
2.466AspAsn: 2.466 ± 0.021
2.017AspPro: 2.017 ± 0.017
2.077AspGln: 2.077 ± 0.018
1.891AspArg: 1.891 ± 0.018
4.215AspSer: 4.215 ± 0.027
2.569AspThr: 2.569 ± 0.02
2.95AspVal: 2.95 ± 0.023
0.504AspTrp: 0.504 ± 0.009
1.832AspTyr: 1.832 ± 0.017
0.001AspXaa: 0.001 ± 0.0
Glu
2.813GluAla: 2.813 ± 0.028
0.946GluCys: 0.946 ± 0.012
3.883GluAsp: 3.883 ± 0.027
7.322GluGlu: 7.322 ± 0.065
2.824GluPhe: 2.824 ± 0.02
2.435GluGly: 2.435 ± 0.021
1.613GluHis: 1.613 ± 0.015
4.416GluIle: 4.416 ± 0.031
6.48GluLys: 6.48 ± 0.052
6.05GluLeu: 6.05 ± 0.042
2.012GluMet: 2.012 ± 0.019
4.452GluAsn: 4.452 ± 0.029
1.693GluPro: 1.693 ± 0.016
3.403GluGln: 3.403 ± 0.026
3.195GluArg: 3.195 ± 0.026
4.772GluSer: 4.772 ± 0.028
3.558GluThr: 3.558 ± 0.026
2.904GluVal: 2.904 ± 0.021
0.679GluTrp: 0.679 ± 0.01
2.3GluTyr: 2.3 ± 0.019
0.001GluXaa: 0.001 ± 0.0
Phe
2.1PheAla: 2.1 ± 0.019
0.779PheCys: 0.779 ± 0.013
2.658PheAsp: 2.658 ± 0.021
3.154PheGlu: 3.154 ± 0.022
2.131PhePhe: 2.131 ± 0.024
2.529PheGly: 2.529 ± 0.021
1.231PheHis: 1.231 ± 0.014
3.137PheIle: 3.137 ± 0.028
3.169PheLys: 3.169 ± 0.022
4.114PheLeu: 4.114 ± 0.032
1.157PheMet: 1.157 ± 0.011
2.692PheAsn: 2.692 ± 0.022
1.659PhePro: 1.659 ± 0.016
1.775PheGln: 1.775 ± 0.017
1.648PheArg: 1.648 ± 0.017
4.523PheSer: 4.523 ± 0.027
2.573PheThr: 2.573 ± 0.022
2.968PheVal: 2.968 ± 0.028
0.412PheTrp: 0.412 ± 0.009
1.691PheTyr: 1.691 ± 0.018
0.001PheXaa: 0.001 ± 0.0
Gly
2.143GlyAla: 2.143 ± 0.023
0.741GlyCys: 0.741 ± 0.012
2.334GlyAsp: 2.334 ± 0.019
2.434GlyGlu: 2.434 ± 0.018
2.195GlyPhe: 2.195 ± 0.02
3.392GlyGly: 3.392 ± 0.045
1.065GlyHis: 1.065 ± 0.014
2.87GlyIle: 2.87 ± 0.021
2.977GlyLys: 2.977 ± 0.021
4.659GlyLeu: 4.659 ± 0.209
1.102GlyMet: 1.102 ± 0.015
2.574GlyAsn: 2.574 ± 0.023
1.169GlyPro: 1.169 ± 0.071
1.472GlyGln: 1.472 ± 0.016
1.687GlyArg: 1.687 ± 0.021
4.043GlySer: 4.043 ± 0.034
2.514GlyThr: 2.514 ± 0.024
2.781GlyVal: 2.781 ± 0.025
0.415GlyTrp: 0.415 ± 0.008
1.569GlyTyr: 1.569 ± 0.017
0.001GlyXaa: 0.001 ± 0.0
His
1.214HisAla: 1.214 ± 0.014
0.451HisCys: 0.451 ± 0.009
1.5HisAsp: 1.5 ± 0.017
1.696HisGlu: 1.696 ± 0.016
1.231HisPhe: 1.231 ± 0.014
1.143HisGly: 1.143 ± 0.014
2.2HisHis: 2.2 ± 0.032
1.428HisIle: 1.428 ± 0.014
1.345HisLys: 1.345 ± 0.015
2.565HisLeu: 2.565 ± 0.02
0.545HisMet: 0.545 ± 0.008
1.454HisAsn: 1.454 ± 0.017
1.39HisPro: 1.39 ± 0.017
1.55HisGln: 1.55 ± 0.016
1.059HisArg: 1.059 ± 0.012
2.863HisSer: 2.863 ± 0.025
1.432HisThr: 1.432 ± 0.016
1.638HisVal: 1.638 ± 0.016
0.213HisTrp: 0.213 ± 0.005
0.988HisTyr: 0.988 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
3.256IleAla: 3.256 ± 0.024
1.076IleCys: 1.076 ± 0.012
3.492IleAsp: 3.492 ± 0.023
4.517IleGlu: 4.517 ± 0.029
2.883IlePhe: 2.883 ± 0.027
2.991IleGly: 2.991 ± 0.024
1.916IleHis: 1.916 ± 0.017
4.16IleIle: 4.16 ± 0.027
3.713IleLys: 3.713 ± 0.024
6.103IleLeu: 6.103 ± 0.04
1.427IleMet: 1.427 ± 0.013
3.011IleAsn: 3.011 ± 0.023
3.242IlePro: 3.242 ± 0.022
3.091IleGln: 3.091 ± 0.021
2.598IleArg: 2.598 ± 0.02
5.879IleSer: 5.879 ± 0.032
3.677IleThr: 3.677 ± 0.025
3.852IleVal: 3.852 ± 0.025
0.472IleTrp: 0.472 ± 0.009
2.272IleTyr: 2.272 ± 0.021
0.0IleXaa: 0.0 ± 0.0
Lys
3.201LysAla: 3.201 ± 0.028
1.023LysCys: 1.023 ± 0.016
3.709LysAsp: 3.709 ± 0.03
5.85LysGlu: 5.85 ± 0.046
2.801LysPhe: 2.801 ± 0.02
2.553LysGly: 2.553 ± 0.023
1.794LysHis: 1.794 ± 0.018
4.556LysIle: 4.556 ± 0.027
7.754LysLys: 7.754 ± 0.06
6.378LysLeu: 6.378 ± 0.043
1.725LysMet: 1.725 ± 0.016
4.249LysAsn: 4.249 ± 0.024
2.677LysPro: 2.677 ± 0.069
3.948LysGln: 3.948 ± 0.032
3.767LysArg: 3.767 ± 0.022
5.486LysSer: 5.486 ± 0.033
4.249LysThr: 4.249 ± 0.028
3.442LysVal: 3.442 ± 0.025
0.603LysTrp: 0.603 ± 0.01
2.691LysTyr: 2.691 ± 0.023
0.002LysXaa: 0.002 ± 0.0
Leu
4.226LeuAla: 4.226 ± 0.025
1.507LeuCys: 1.507 ± 0.014
4.585LeuAsp: 4.585 ± 0.03
6.257LeuGlu: 6.257 ± 0.048
4.824LeuPhe: 4.824 ± 0.034
4.728LeuGly: 4.728 ± 0.211
2.216LeuHis: 2.216 ± 0.02
5.483LeuIle: 5.483 ± 0.031
7.336LeuLys: 7.336 ± 0.051
9.361LeuLeu: 9.361 ± 0.054
2.225LeuMet: 2.225 ± 0.02
5.607LeuAsn: 5.607 ± 0.034
3.652LeuPro: 3.652 ± 0.023
4.283LeuGln: 4.283 ± 0.028
3.869LeuArg: 3.869 ± 0.029
8.779LeuSer: 8.779 ± 0.046
5.047LeuThr: 5.047 ± 0.03
4.852LeuVal: 4.852 ± 0.032
0.736LeuTrp: 0.736 ± 0.011
3.072LeuTyr: 3.072 ± 0.025
0.0LeuXaa: 0.0 ± 0.0
Met
1.152MetAla: 1.152 ± 0.013
0.321MetCys: 0.321 ± 0.007
1.404MetAsp: 1.404 ± 0.014
1.629MetGlu: 1.629 ± 0.016
1.034MetPhe: 1.034 ± 0.013
0.949MetGly: 0.949 ± 0.012
0.458MetHis: 0.458 ± 0.008
1.747MetIle: 1.747 ± 0.016
2.22MetLys: 2.22 ± 0.02
1.986MetLeu: 1.986 ± 0.018
1.058MetMet: 1.058 ± 0.016
2.027MetAsn: 2.027 ± 0.019
0.746MetPro: 0.746 ± 0.011
0.914MetGln: 0.914 ± 0.012
0.931MetArg: 0.931 ± 0.011
2.137MetSer: 2.137 ± 0.018
1.581MetThr: 1.581 ± 0.015
1.253MetVal: 1.253 ± 0.015
0.184MetTrp: 0.184 ± 0.005
0.757MetTyr: 0.757 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.861AsnAla: 2.861 ± 0.021
0.902AsnCys: 0.902 ± 0.015
3.51AsnAsp: 3.51 ± 0.026
4.038AsnGlu: 4.038 ± 0.027
2.486AsnPhe: 2.486 ± 0.019
2.881AsnGly: 2.881 ± 0.025
2.078AsnHis: 2.078 ± 0.022
4.17AsnIle: 4.17 ± 0.029
3.393AsnLys: 3.393 ± 0.024
4.885AsnLeu: 4.885 ± 0.031
1.458AsnMet: 1.458 ± 0.013
6.013AsnAsn: 6.013 ± 0.057
2.488AsnPro: 2.488 ± 0.021
2.608AsnGln: 2.608 ± 0.022
2.206AsnArg: 2.206 ± 0.02
6.078AsnSer: 6.078 ± 0.043
4.174AsnThr: 4.174 ± 0.034
3.414AsnVal: 3.414 ± 0.025
0.435AsnTrp: 0.435 ± 0.009
2.09AsnTyr: 2.09 ± 0.019
0.001AsnXaa: 0.001 ± 0.0
Pro
1.341ProAla: 1.341 ± 0.013
0.479ProCys: 0.479 ± 0.009
1.27ProAsp: 1.27 ± 0.013
2.098ProGlu: 2.098 ± 0.018
2.033ProPhe: 2.033 ± 0.018
1.249ProGly: 1.249 ± 0.07
1.151ProHis: 1.151 ± 0.013
2.592ProIle: 2.592 ± 0.019
2.857ProLys: 2.857 ± 0.068
3.734ProLeu: 3.734 ± 0.024
0.823ProMet: 0.823 ± 0.011
2.605ProAsn: 2.605 ± 0.027
2.983ProPro: 2.983 ± 0.05
2.221ProGln: 2.221 ± 0.026
1.373ProArg: 1.373 ± 0.017
5.379ProSer: 5.379 ± 0.04
3.069ProThr: 3.069 ± 0.027
1.972ProVal: 1.972 ± 0.019
0.245ProTrp: 0.245 ± 0.006
1.353ProTyr: 1.353 ± 0.014
0.001ProXaa: 0.001 ± 0.0
Gln
2.05GlnAla: 2.05 ± 0.019
0.639GlnCys: 0.639 ± 0.012
2.019GlnAsp: 2.019 ± 0.016
3.266GlnGlu: 3.266 ± 0.029
2.073GlnPhe: 2.073 ± 0.018
1.427GlnGly: 1.427 ± 0.015
1.652GlnHis: 1.652 ± 0.018
2.68GlnIle: 2.68 ± 0.02
3.289GlnLys: 3.289 ± 0.031
4.284GlnLeu: 4.284 ± 0.033
1.131GlnMet: 1.131 ± 0.013
2.669GlnAsn: 2.669 ± 0.025
2.179GlnPro: 2.179 ± 0.027
5.145GlnGln: 5.145 ± 0.061
1.917GlnArg: 1.917 ± 0.017
3.561GlnSer: 3.561 ± 0.025
2.659GlnThr: 2.659 ± 0.021
2.477GlnVal: 2.477 ± 0.021
0.366GlnTrp: 0.366 ± 0.007
1.609GlnTyr: 1.609 ± 0.014
0.001GlnXaa: 0.001 ± 0.0
Arg
1.806ArgAla: 1.806 ± 0.017
0.565ArgCys: 0.565 ± 0.01
2.045ArgAsp: 2.045 ± 0.019
2.965ArgGlu: 2.965 ± 0.027
1.843ArgPhe: 1.843 ± 0.017
1.756ArgGly: 1.756 ± 0.019
0.941ArgHis: 0.941 ± 0.013
2.601ArgIle: 2.601 ± 0.021
3.84ArgLys: 3.84 ± 0.027
3.45ArgLeu: 3.45 ± 0.024
1.116ArgMet: 1.116 ± 0.012
2.682ArgAsn: 2.682 ± 0.021
1.318ArgPro: 1.318 ± 0.016
1.564ArgGln: 1.564 ± 0.017
2.238ArgArg: 2.238 ± 0.023
3.127ArgSer: 3.127 ± 0.021
2.076ArgThr: 2.076 ± 0.018
2.341ArgVal: 2.341 ± 0.019
0.361ArgTrp: 0.361 ± 0.007
1.371ArgTyr: 1.371 ± 0.015
0.0ArgXaa: 0.0 ± 0.0
Ser
4.012SerAla: 4.012 ± 0.028
1.462SerCys: 1.462 ± 0.017
4.332SerAsp: 4.332 ± 0.026
5.295SerGlu: 5.295 ± 0.028
4.315SerPhe: 4.315 ± 0.031
4.146SerGly: 4.146 ± 0.035
2.556SerHis: 2.556 ± 0.022
5.844SerIle: 5.844 ± 0.034
6.188SerLys: 6.188 ± 0.034
8.716SerLeu: 8.716 ± 0.04
2.092SerMet: 2.092 ± 0.018
6.594SerAsn: 6.594 ± 0.04
4.467SerPro: 4.467 ± 0.04
4.265SerGln: 4.265 ± 0.028
3.231SerArg: 3.231 ± 0.021
16.863SerSer: 16.863 ± 0.124
7.253SerThr: 7.253 ± 0.048
4.702SerVal: 4.702 ± 0.03
0.604SerTrp: 0.604 ± 0.009
2.532SerTyr: 2.532 ± 0.02
0.001SerXaa: 0.001 ± 0.0
Thr
2.807ThrAla: 2.807 ± 0.025
1.104ThrCys: 1.104 ± 0.017
2.45ThrAsp: 2.45 ± 0.021
3.085ThrGlu: 3.085 ± 0.022
2.827ThrPhe: 2.827 ± 0.02
2.338ThrGly: 2.338 ± 0.02
1.469ThrHis: 1.469 ± 0.015
4.122ThrIle: 4.122 ± 0.028
3.942ThrLys: 3.942 ± 0.03
5.748ThrLeu: 5.748 ± 0.033
1.327ThrMet: 1.327 ± 0.015
4.179ThrAsn: 4.179 ± 0.03
3.35ThrPro: 3.35 ± 0.033
2.447ThrGln: 2.447 ± 0.017
2.23ThrArg: 2.23 ± 0.017
7.304ThrSer: 7.304 ± 0.048
7.823ThrThr: 7.823 ± 0.093
2.963ThrVal: 2.963 ± 0.026
0.417ThrTrp: 0.417 ± 0.008
1.539ThrTyr: 1.539 ± 0.017
0.001ThrXaa: 0.001 ± 0.0
Val
2.842ValAla: 2.842 ± 0.024
1.003ValCys: 1.003 ± 0.022
2.964ValAsp: 2.964 ± 0.023
3.543ValGlu: 3.543 ± 0.025
2.675ValPhe: 2.675 ± 0.025
2.471ValGly: 2.471 ± 0.025
1.294ValHis: 1.294 ± 0.015
3.509ValIle: 3.509 ± 0.026
3.933ValLys: 3.933 ± 0.026
5.259ValLeu: 5.259 ± 0.033
1.44ValMet: 1.44 ± 0.015
3.087ValAsn: 3.087 ± 0.023
2.12ValPro: 2.12 ± 0.02
2.164ValGln: 2.164 ± 0.017
2.068ValArg: 2.068 ± 0.018
4.597ValSer: 4.597 ± 0.027
3.2ValThr: 3.2 ± 0.026
3.834ValVal: 3.834 ± 0.028
0.506ValTrp: 0.506 ± 0.012
1.72ValTyr: 1.72 ± 0.018
0.0ValXaa: 0.0 ± 0.0
Trp
0.318TrpAla: 0.318 ± 0.012
0.147TrpCys: 0.147 ± 0.005
0.418TrpAsp: 0.418 ± 0.008
0.44TrpGlu: 0.44 ± 0.008
0.417TrpPhe: 0.417 ± 0.008
0.317TrpGly: 0.317 ± 0.006
0.155TrpHis: 0.155 ± 0.005
0.66TrpIle: 0.66 ± 0.011
0.835TrpLys: 0.835 ± 0.011
0.703TrpLeu: 0.703 ± 0.012
0.283TrpMet: 0.283 ± 0.006
0.664TrpAsn: 0.664 ± 0.01
0.197TrpPro: 0.197 ± 0.005
0.252TrpGln: 0.252 ± 0.006
0.407TrpArg: 0.407 ± 0.009
0.622TrpSer: 0.622 ± 0.01
0.457TrpThr: 0.457 ± 0.009
0.423TrpVal: 0.423 ± 0.013
0.104TrpTrp: 0.104 ± 0.004
0.306TrpTyr: 0.306 ± 0.007
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.487TyrAla: 1.487 ± 0.018
0.71TyrCys: 0.71 ± 0.01
1.837TyrAsp: 1.837 ± 0.017
2.156TyrGlu: 2.156 ± 0.02
1.891TyrPhe: 1.891 ± 0.019
1.772TyrGly: 1.772 ± 0.022
1.043TyrHis: 1.043 ± 0.014
1.999TyrIle: 1.999 ± 0.021
1.931TyrLys: 1.931 ± 0.016
3.452TyrLeu: 3.452 ± 0.027
0.794TyrMet: 0.794 ± 0.011
1.832TyrAsn: 1.832 ± 0.018
1.252TyrPro: 1.252 ± 0.017
1.427TyrGln: 1.427 ± 0.016
1.368TyrArg: 1.368 ± 0.016
2.968TyrSer: 2.968 ± 0.023
1.534TyrThr: 1.534 ± 0.016
1.798TyrVal: 1.798 ± 0.017
0.349TyrTrp: 0.349 ± 0.007
1.575TyrTyr: 1.575 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.001XaaGlu: 0.001 ± 0.0
0.001XaaPhe: 0.001 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.0
0.002XaaLys: 0.002 ± 0.001
0.001XaaLeu: 0.001 ± 0.0
0.001XaaMet: 0.001 ± 0.0
0.001XaaAsn: 0.001 ± 0.0
0.001XaaPro: 0.001 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.0
0.001XaaSer: 0.001 ± 0.0
0.001XaaThr: 0.001 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.032XaaXaa: 0.032 ± 0.003
Statistics based on 13596 proteins (7262538 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski