Amino acid dipepetide frequency for Bacteriophage vB_NpeS-2AV2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.097AlaAla: 6.097 ± 0.599
0.581AlaCys: 0.581 ± 0.136
4.234AlaAsp: 4.234 ± 0.348
5.395AlaGlu: 5.395 ± 0.556
2.274AlaPhe: 2.274 ± 0.205
4.427AlaGly: 4.427 ± 0.447
0.847AlaHis: 0.847 ± 0.148
5.903AlaIle: 5.903 ± 0.398
5.419AlaLys: 5.419 ± 0.51
6.75AlaLeu: 6.75 ± 0.505
1.669AlaMet: 1.669 ± 0.181
3.508AlaAsn: 3.508 ± 0.253
2.54AlaPro: 2.54 ± 0.295
3.145AlaGln: 3.145 ± 0.503
3.073AlaArg: 3.073 ± 0.253
4.693AlaSer: 4.693 ± 0.436
4.306AlaThr: 4.306 ± 0.518
4.935AlaVal: 4.935 ± 0.358
0.871AlaTrp: 0.871 ± 0.161
1.839AlaTyr: 1.839 ± 0.165
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.093
0.266CysCys: 0.266 ± 0.079
0.895CysAsp: 0.895 ± 0.153
0.895CysGlu: 0.895 ± 0.171
0.702CysPhe: 0.702 ± 0.146
0.944CysGly: 0.944 ± 0.19
0.315CysHis: 0.315 ± 0.089
0.46CysIle: 0.46 ± 0.103
0.968CysLys: 0.968 ± 0.209
1.089CysLeu: 1.089 ± 0.2
0.218CysMet: 0.218 ± 0.08
0.508CysAsn: 0.508 ± 0.111
0.508CysPro: 0.508 ± 0.124
0.532CysGln: 0.532 ± 0.162
0.387CysArg: 0.387 ± 0.093
0.798CysSer: 0.798 ± 0.159
0.46CysThr: 0.46 ± 0.122
0.653CysVal: 0.653 ± 0.142
0.194CysTrp: 0.194 ± 0.068
0.629CysTyr: 0.629 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
4.089AspAla: 4.089 ± 0.35
0.653AspCys: 0.653 ± 0.135
3.653AspAsp: 3.653 ± 0.279
3.484AspGlu: 3.484 ± 0.311
2.879AspPhe: 2.879 ± 0.261
4.089AspGly: 4.089 ± 0.367
0.798AspHis: 0.798 ± 0.174
4.064AspIle: 4.064 ± 0.403
4.863AspLys: 4.863 ± 0.387
5.952AspLeu: 5.952 ± 0.415
0.895AspMet: 0.895 ± 0.182
2.831AspAsn: 2.831 ± 0.256
2.492AspPro: 2.492 ± 0.4
2.226AspGln: 2.226 ± 0.292
3.29AspArg: 3.29 ± 0.274
3.919AspSer: 3.919 ± 0.328
3.339AspThr: 3.339 ± 0.334
3.823AspVal: 3.823 ± 0.363
0.919AspTrp: 0.919 ± 0.168
2.734AspTyr: 2.734 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
5.419GluAla: 5.419 ± 0.518
0.774GluCys: 0.774 ± 0.126
3.387GluAsp: 3.387 ± 0.32
3.46GluGlu: 3.46 ± 0.397
2.952GluPhe: 2.952 ± 0.312
3.024GluGly: 3.024 ± 0.272
0.895GluHis: 0.895 ± 0.173
5.081GluIle: 5.081 ± 0.352
4.137GluLys: 4.137 ± 0.425
7.79GluLeu: 7.79 ± 0.436
1.5GluMet: 1.5 ± 0.245
2.952GluAsn: 2.952 ± 0.346
2.976GluPro: 2.976 ± 0.406
3.0GluGln: 3.0 ± 0.459
3.532GluArg: 3.532 ± 0.295
4.379GluSer: 4.379 ± 0.358
3.871GluThr: 3.871 ± 0.392
4.96GluVal: 4.96 ± 0.375
0.653GluTrp: 0.653 ± 0.125
2.855GluTyr: 2.855 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
1.887PheAla: 1.887 ± 0.215
0.46PheCys: 0.46 ± 0.134
2.952PheAsp: 2.952 ± 0.282
2.129PheGlu: 2.129 ± 0.248
1.234PhePhe: 1.234 ± 0.181
2.419PheGly: 2.419 ± 0.261
0.508PheHis: 0.508 ± 0.108
2.323PheIle: 2.323 ± 0.259
2.855PheLys: 2.855 ± 0.301
3.242PheLeu: 3.242 ± 0.264
0.919PheMet: 0.919 ± 0.156
2.613PheAsn: 2.613 ± 0.261
1.814PhePro: 1.814 ± 0.233
1.79PheGln: 1.79 ± 0.182
1.548PheArg: 1.548 ± 0.206
2.903PheSer: 2.903 ± 0.195
3.193PheThr: 3.193 ± 0.277
2.105PheVal: 2.105 ± 0.241
0.315PheTrp: 0.315 ± 0.112
1.524PheTyr: 1.524 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
4.016GlyAla: 4.016 ± 0.346
0.992GlyCys: 0.992 ± 0.217
4.089GlyAsp: 4.089 ± 0.283
4.21GlyGlu: 4.21 ± 0.345
2.831GlyPhe: 2.831 ± 0.314
3.919GlyGly: 3.919 ± 0.314
0.992GlyHis: 0.992 ± 0.176
3.871GlyIle: 3.871 ± 0.28
4.306GlyLys: 4.306 ± 0.407
5.734GlyLeu: 5.734 ± 0.363
1.161GlyMet: 1.161 ± 0.179
3.121GlyAsn: 3.121 ± 0.288
0.0GlyPro: 0.0 ± 0.0
2.395GlyGln: 2.395 ± 0.277
2.71GlyArg: 2.71 ± 0.308
4.693GlySer: 4.693 ± 0.335
3.702GlyThr: 3.702 ± 0.398
4.04GlyVal: 4.04 ± 0.314
0.992GlyTrp: 0.992 ± 0.196
2.855GlyTyr: 2.855 ± 0.297
0.0GlyXaa: 0.0 ± 0.0
His
0.847HisAla: 0.847 ± 0.165
0.339HisCys: 0.339 ± 0.091
0.726HisAsp: 0.726 ± 0.166
0.895HisGlu: 0.895 ± 0.158
0.726HisPhe: 0.726 ± 0.138
0.726HisGly: 0.726 ± 0.126
0.339HisHis: 0.339 ± 0.094
0.847HisIle: 0.847 ± 0.145
1.185HisLys: 1.185 ± 0.186
1.621HisLeu: 1.621 ± 0.275
0.121HisMet: 0.121 ± 0.051
0.774HisAsn: 0.774 ± 0.125
0.823HisPro: 0.823 ± 0.153
0.895HisGln: 0.895 ± 0.166
0.919HisArg: 0.919 ± 0.17
0.871HisSer: 0.871 ± 0.189
0.798HisThr: 0.798 ± 0.164
0.653HisVal: 0.653 ± 0.133
0.169HisTrp: 0.169 ± 0.092
0.798HisTyr: 0.798 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
6.581IleAla: 6.581 ± 0.353
0.605IleCys: 0.605 ± 0.138
4.258IleAsp: 4.258 ± 0.332
4.331IleGlu: 4.331 ± 0.371
1.911IlePhe: 1.911 ± 0.249
3.484IleGly: 3.484 ± 0.39
0.871IleHis: 0.871 ± 0.182
2.855IleIle: 2.855 ± 0.253
4.911IleLys: 4.911 ± 0.339
4.379IleLeu: 4.379 ± 0.402
0.847IleMet: 0.847 ± 0.152
3.339IleAsn: 3.339 ± 0.277
2.637IlePro: 2.637 ± 0.326
2.564IleGln: 2.564 ± 0.227
2.903IleArg: 2.903 ± 0.28
4.887IleSer: 4.887 ± 0.439
4.137IleThr: 4.137 ± 0.365
3.556IleVal: 3.556 ± 0.258
0.556IleTrp: 0.556 ± 0.148
2.153IleTyr: 2.153 ± 0.281
0.0IleXaa: 0.0 ± 0.0
Lys
5.202LysAla: 5.202 ± 0.425
0.75LysCys: 0.75 ± 0.129
4.089LysAsp: 4.089 ± 0.449
4.935LysGlu: 4.935 ± 0.352
2.952LysPhe: 2.952 ± 0.295
3.871LysGly: 3.871 ± 0.377
1.04LysHis: 1.04 ± 0.182
3.943LysIle: 3.943 ± 0.278
5.25LysLys: 5.25 ± 0.505
7.887LysLeu: 7.887 ± 0.605
1.258LysMet: 1.258 ± 0.204
2.879LysAsn: 2.879 ± 0.264
3.871LysPro: 3.871 ± 0.387
3.677LysGln: 3.677 ± 0.387
3.726LysArg: 3.726 ± 0.422
5.661LysSer: 5.661 ± 0.39
4.452LysThr: 4.452 ± 0.499
5.202LysVal: 5.202 ± 0.403
0.653LysTrp: 0.653 ± 0.119
2.081LysTyr: 2.081 ± 0.235
0.0LysXaa: 0.0 ± 0.0
Leu
7.572LeuAla: 7.572 ± 0.535
0.847LeuCys: 0.847 ± 0.172
5.831LeuAsp: 5.831 ± 0.339
6.702LeuGlu: 6.702 ± 0.382
3.169LeuPhe: 3.169 ± 0.287
5.516LeuGly: 5.516 ± 0.424
1.5LeuHis: 1.5 ± 0.242
5.226LeuIle: 5.226 ± 0.394
6.556LeuLys: 6.556 ± 0.483
8.105LeuLeu: 8.105 ± 0.517
1.258LeuMet: 1.258 ± 0.193
5.274LeuAsn: 5.274 ± 0.418
4.524LeuPro: 4.524 ± 0.359
4.621LeuGln: 4.621 ± 0.411
4.089LeuArg: 4.089 ± 0.33
6.46LeuSer: 6.46 ± 0.33
6.193LeuThr: 6.193 ± 0.435
5.564LeuVal: 5.564 ± 0.331
0.992LeuTrp: 0.992 ± 0.181
2.637LeuTyr: 2.637 ± 0.251
0.0LeuXaa: 0.0 ± 0.0
Met
1.258MetAla: 1.258 ± 0.195
0.508MetCys: 0.508 ± 0.113
0.823MetAsp: 0.823 ± 0.155
1.064MetGlu: 1.064 ± 0.148
0.653MetPhe: 0.653 ± 0.135
0.992MetGly: 0.992 ± 0.171
0.169MetHis: 0.169 ± 0.082
1.282MetIle: 1.282 ± 0.183
1.282MetLys: 1.282 ± 0.252
1.839MetLeu: 1.839 ± 0.178
0.339MetMet: 0.339 ± 0.085
0.653MetAsn: 0.653 ± 0.13
0.798MetPro: 0.798 ± 0.146
0.774MetGln: 0.774 ± 0.128
0.75MetArg: 0.75 ± 0.163
1.79MetSer: 1.79 ± 0.24
1.258MetThr: 1.258 ± 0.19
1.016MetVal: 1.016 ± 0.142
0.073MetTrp: 0.073 ± 0.041
0.726MetTyr: 0.726 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.193AsnAla: 3.193 ± 0.254
0.944AsnCys: 0.944 ± 0.173
2.444AsnAsp: 2.444 ± 0.261
2.806AsnGlu: 2.806 ± 0.207
2.153AsnPhe: 2.153 ± 0.223
3.363AsnGly: 3.363 ± 0.286
0.605AsnHis: 0.605 ± 0.136
2.927AsnIle: 2.927 ± 0.251
3.726AsnLys: 3.726 ± 0.302
4.935AsnLeu: 4.935 ± 0.384
0.847AsnMet: 0.847 ± 0.143
2.516AsnAsn: 2.516 ± 0.227
3.29AsnPro: 3.29 ± 0.28
2.468AsnGln: 2.468 ± 0.306
2.153AsnArg: 2.153 ± 0.255
3.484AsnSer: 3.484 ± 0.458
2.613AsnThr: 2.613 ± 0.278
2.661AsnVal: 2.661 ± 0.24
0.677AsnTrp: 0.677 ± 0.116
2.008AsnTyr: 2.008 ± 0.221
0.0AsnXaa: 0.0 ± 0.0
Pro
2.734ProAla: 2.734 ± 0.313
0.532ProCys: 0.532 ± 0.134
3.169ProAsp: 3.169 ± 0.313
4.185ProGlu: 4.185 ± 0.471
1.403ProPhe: 1.403 ± 0.165
2.613ProGly: 2.613 ± 0.269
0.847ProHis: 0.847 ± 0.144
1.96ProIle: 1.96 ± 0.205
3.411ProLys: 3.411 ± 0.32
2.613ProLeu: 2.613 ± 0.244
0.653ProMet: 0.653 ± 0.138
2.202ProAsn: 2.202 ± 0.245
1.452ProPro: 1.452 ± 0.29
2.032ProGln: 2.032 ± 0.215
1.137ProArg: 1.137 ± 0.133
2.927ProSer: 2.927 ± 0.291
3.193ProThr: 3.193 ± 0.318
2.782ProVal: 2.782 ± 0.255
0.387ProTrp: 0.387 ± 0.103
1.282ProTyr: 1.282 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
3.556GlnAla: 3.556 ± 0.414
0.339GlnCys: 0.339 ± 0.093
2.056GlnAsp: 2.056 ± 0.2
3.702GlnGlu: 3.702 ± 0.398
1.694GlnPhe: 1.694 ± 0.225
2.419GlnGly: 2.419 ± 0.216
0.75GlnHis: 0.75 ± 0.141
3.774GlnIle: 3.774 ± 0.296
2.637GlnLys: 2.637 ± 0.326
5.177GlnLeu: 5.177 ± 0.603
0.895GlnMet: 0.895 ± 0.154
1.669GlnAsn: 1.669 ± 0.214
2.419GlnPro: 2.419 ± 0.377
3.411GlnGln: 3.411 ± 0.445
1.935GlnArg: 1.935 ± 0.252
3.121GlnSer: 3.121 ± 0.445
2.492GlnThr: 2.492 ± 0.299
3.024GlnVal: 3.024 ± 0.292
0.435GlnTrp: 0.435 ± 0.116
1.282GlnTyr: 1.282 ± 0.139
0.0GlnXaa: 0.0 ± 0.0
Arg
2.661ArgAla: 2.661 ± 0.199
0.508ArgCys: 0.508 ± 0.122
2.831ArgAsp: 2.831 ± 0.268
3.508ArgGlu: 3.508 ± 0.326
1.79ArgPhe: 1.79 ± 0.2
2.613ArgGly: 2.613 ± 0.278
0.871ArgHis: 0.871 ± 0.186
2.444ArgIle: 2.444 ± 0.254
3.774ArgLys: 3.774 ± 0.336
4.935ArgLeu: 4.935 ± 0.394
0.944ArgMet: 0.944 ± 0.178
2.25ArgAsn: 2.25 ± 0.209
1.282ArgPro: 1.282 ± 0.185
2.129ArgGln: 2.129 ± 0.246
2.468ArgArg: 2.468 ± 0.257
3.629ArgSer: 3.629 ± 0.275
2.274ArgThr: 2.274 ± 0.234
3.387ArgVal: 3.387 ± 0.287
0.508ArgTrp: 0.508 ± 0.12
1.694ArgTyr: 1.694 ± 0.21
0.0ArgXaa: 0.0 ± 0.0
Ser
4.379SerAla: 4.379 ± 0.372
0.484SerCys: 0.484 ± 0.103
5.129SerAsp: 5.129 ± 0.506
4.524SerGlu: 4.524 ± 0.349
3.097SerPhe: 3.097 ± 0.244
5.492SerGly: 5.492 ± 0.42
1.137SerHis: 1.137 ± 0.177
3.919SerIle: 3.919 ± 0.329
5.322SerLys: 5.322 ± 0.481
6.29SerLeu: 6.29 ± 0.435
1.161SerMet: 1.161 ± 0.18
3.847SerAsn: 3.847 ± 0.311
2.976SerPro: 2.976 ± 0.239
3.387SerGln: 3.387 ± 0.516
3.46SerArg: 3.46 ± 0.338
4.403SerSer: 4.403 ± 0.495
4.452SerThr: 4.452 ± 0.414
4.306SerVal: 4.306 ± 0.295
0.508SerTrp: 0.508 ± 0.105
2.032SerTyr: 2.032 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
5.177ThrAla: 5.177 ± 0.374
0.581ThrCys: 0.581 ± 0.144
3.435ThrAsp: 3.435 ± 0.326
3.847ThrGlu: 3.847 ± 0.366
2.177ThrPhe: 2.177 ± 0.301
3.702ThrGly: 3.702 ± 0.257
1.064ThrHis: 1.064 ± 0.178
4.403ThrIle: 4.403 ± 0.358
4.669ThrLys: 4.669 ± 0.429
5.395ThrLeu: 5.395 ± 0.311
0.677ThrMet: 0.677 ± 0.135
3.097ThrAsn: 3.097 ± 0.282
3.314ThrPro: 3.314 ± 0.355
3.024ThrGln: 3.024 ± 0.31
2.71ThrArg: 2.71 ± 0.327
4.161ThrSer: 4.161 ± 0.316
4.403ThrThr: 4.403 ± 0.536
4.016ThrVal: 4.016 ± 0.427
0.508ThrTrp: 0.508 ± 0.12
2.129ThrTyr: 2.129 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
5.129ValAla: 5.129 ± 0.324
0.847ValCys: 0.847 ± 0.154
4.161ValAsp: 4.161 ± 0.267
5.105ValGlu: 5.105 ± 0.309
2.177ValPhe: 2.177 ± 0.237
3.798ValGly: 3.798 ± 0.314
0.75ValHis: 0.75 ± 0.154
3.919ValIle: 3.919 ± 0.426
4.355ValLys: 4.355 ± 0.413
4.693ValLeu: 4.693 ± 0.343
1.573ValMet: 1.573 ± 0.237
3.605ValAsn: 3.605 ± 0.271
2.202ValPro: 2.202 ± 0.231
2.492ValGln: 2.492 ± 0.275
3.097ValArg: 3.097 ± 0.293
4.282ValSer: 4.282 ± 0.301
4.621ValThr: 4.621 ± 0.333
4.161ValVal: 4.161 ± 0.355
0.75ValTrp: 0.75 ± 0.157
1.911ValTyr: 1.911 ± 0.218
0.0ValXaa: 0.0 ± 0.0
Trp
0.532TrpAla: 0.532 ± 0.137
0.242TrpCys: 0.242 ± 0.078
0.944TrpAsp: 0.944 ± 0.202
0.702TrpGlu: 0.702 ± 0.129
0.339TrpPhe: 0.339 ± 0.096
0.895TrpGly: 0.895 ± 0.163
0.169TrpHis: 0.169 ± 0.063
0.556TrpIle: 0.556 ± 0.098
0.823TrpLys: 0.823 ± 0.134
1.064TrpLeu: 1.064 ± 0.17
0.29TrpMet: 0.29 ± 0.084
0.508TrpAsn: 0.508 ± 0.114
0.073TrpPro: 0.073 ± 0.039
0.484TrpGln: 0.484 ± 0.119
0.726TrpArg: 0.726 ± 0.14
0.653TrpSer: 0.653 ± 0.141
0.363TrpThr: 0.363 ± 0.091
1.089TrpVal: 1.089 ± 0.192
0.218TrpTrp: 0.218 ± 0.08
0.363TrpTyr: 0.363 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.839TyrAla: 1.839 ± 0.192
0.508TyrCys: 0.508 ± 0.105
1.911TyrAsp: 1.911 ± 0.186
1.718TyrGlu: 1.718 ± 0.19
1.621TyrPhe: 1.621 ± 0.23
2.323TyrGly: 2.323 ± 0.271
0.605TyrHis: 0.605 ± 0.129
2.008TyrIle: 2.008 ± 0.219
2.927TyrLys: 2.927 ± 0.361
3.145TyrLeu: 3.145 ± 0.284
0.798TyrMet: 0.798 ± 0.163
1.669TyrAsn: 1.669 ± 0.176
1.524TyrPro: 1.524 ± 0.219
1.742TyrGln: 1.742 ± 0.199
1.887TyrArg: 1.887 ± 0.239
2.589TyrSer: 2.589 ± 0.329
2.371TyrThr: 2.371 ± 0.209
1.694TyrVal: 1.694 ± 0.24
0.653TyrTrp: 0.653 ± 0.115
1.282TyrTyr: 1.282 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 182 proteins (41335 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski