Tuber borchii (White truffle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Tuberaceae; Tuber

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6ZW64|A0A2T6ZW64_TUBBO Uncharacterized protein OS=Tuber borchii OX=42251 GN=B9Z19DRAFT_794141 PE=4 SV=1
MM1 pKa = 7.44SISLEE6 pKa = 4.21GEE8 pKa = 4.12HH9 pKa = 6.77KK10 pKa = 10.52CIEE13 pKa = 4.16RR14 pKa = 11.84STEE17 pKa = 4.03CDD19 pKa = 3.33CPICGEE25 pKa = 4.19YY26 pKa = 10.09MFTSTQTVVFMTCGHH41 pKa = 6.78SIHH44 pKa = 5.81QRR46 pKa = 11.84CYY48 pKa = 7.74YY49 pKa = 11.25DD50 pKa = 3.76HH51 pKa = 7.08MKK53 pKa = 9.99TSYY56 pKa = 10.56RR57 pKa = 11.84CPTCARR63 pKa = 11.84TIINMEE69 pKa = 4.04SQFRR73 pKa = 11.84ALDD76 pKa = 3.93LEE78 pKa = 5.09IEE80 pKa = 4.4TQPLPEE86 pKa = 4.99PYY88 pKa = 10.45KK89 pKa = 10.63NWRR92 pKa = 11.84CLIGCNDD99 pKa = 4.28CSAKK103 pKa = 10.91SNVLFHH109 pKa = 7.02FLGLKK114 pKa = 10.26CEE116 pKa = 4.12NCKK119 pKa = 10.66SYY121 pKa = 9.31NTNQIRR127 pKa = 11.84ILRR130 pKa = 11.84PEE132 pKa = 4.66DD133 pKa = 3.75GQDD136 pKa = 3.23GGGGNMSPSAIPRR149 pKa = 11.84IPEE152 pKa = 3.78RR153 pKa = 11.84TTSSNSLLGGQANSPPRR170 pKa = 11.84LAPAEE175 pKa = 4.3GEE177 pKa = 4.05AALANANRR185 pKa = 11.84AIAAAGEE192 pKa = 4.54AIMEE196 pKa = 4.41LDD198 pKa = 3.56GVEE201 pKa = 5.34DD202 pKa = 5.68LIIDD206 pKa = 4.63DD207 pKa = 3.81GWEE210 pKa = 4.22TEE212 pKa = 4.43DD213 pKa = 4.03SADD216 pKa = 3.64YY217 pKa = 11.13TDD219 pKa = 5.59IDD221 pKa = 5.53DD222 pKa = 6.3DD223 pKa = 5.33DD224 pKa = 5.09DD225 pKa = 5.47LSGFGGDD232 pKa = 3.0GVEE235 pKa = 4.48AVEE238 pKa = 4.31EE239 pKa = 4.34DD240 pKa = 4.0SEE242 pKa = 5.0EE243 pKa = 4.12EE244 pKa = 5.17DD245 pKa = 5.07GDD247 pKa = 5.24GDD249 pKa = 6.12DD250 pKa = 6.46DD251 pKa = 7.16DD252 pKa = 7.69DD253 pKa = 7.74DD254 pKa = 7.55DD255 pKa = 7.67DD256 pKa = 7.73DD257 pKa = 7.02DD258 pKa = 7.08DD259 pKa = 5.77LIQLIGHH266 pKa = 6.78RR267 pKa = 4.35

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6ZHB4|A0A2T6ZHB4_TUBBO TFIIF_beta domain-containing protein OS=Tuber borchii OX=42251 GN=B9Z19DRAFT_1132400 PE=4 SV=1
MM1 pKa = 7.38FRR3 pKa = 11.84PILRR7 pKa = 11.84PMLNQSVFRR16 pKa = 11.84PALLGKK22 pKa = 9.31VIRR25 pKa = 11.84PALVLPISPRR35 pKa = 11.84PVPAPVVLFARR46 pKa = 11.84RR47 pKa = 11.84GSSMFLLPNTNLIVLTPPSPPPKK70 pKa = 9.96WMTAALLKK78 pKa = 10.55SSPRR82 pKa = 11.84SMGSFNQLRR91 pKa = 11.84MISKK95 pKa = 9.27WLVTSTSPVSS105 pKa = 3.26

Molecular weight: 11.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12324

0

12324

4666832

49

5527

378.7

41.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.727 ± 0.018

1.279 ± 0.009

5.205 ± 0.016

6.654 ± 0.028

3.712 ± 0.015

7.383 ± 0.023

2.336 ± 0.01

5.095 ± 0.017

5.308 ± 0.022

8.908 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.009

3.668 ± 0.013

6.31 ± 0.03

3.593 ± 0.02

6.244 ± 0.016

8.361 ± 0.025

5.939 ± 0.02

6.079 ± 0.017

1.356 ± 0.008

2.71 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski