Pseudodesulfovibrio piezophilus (strain DSM 21447 / JCM 15486 / C1TLV30) (Desulfovibrio piezophilus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Pseudodesulfovibrio; Pseudodesulfovibrio piezophilus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1WKU8|M1WKU8_PSEP2 Formate dehydrogenase subunit alpha OS=Pseudodesulfovibrio piezophilus (strain DSM 21447 / JCM 15486 / C1TLV30) OX=1322246 GN=fdhA PE=3 SV=1
MM1 pKa = 7.3TRR3 pKa = 11.84FSVQATSSSPHH14 pKa = 7.43IEE16 pKa = 3.98FDD18 pKa = 3.58PEE20 pKa = 4.15TMSFEE25 pKa = 4.54IKK27 pKa = 10.62GEE29 pKa = 4.17SYY31 pKa = 10.87PEE33 pKa = 4.03NCWAFYY39 pKa = 11.2GPMFDD44 pKa = 3.1WLEE47 pKa = 4.16TFFSGINGDD56 pKa = 3.22RR57 pKa = 11.84VEE59 pKa = 4.44INMEE63 pKa = 3.57ILYY66 pKa = 10.36FNSSSSKK73 pKa = 9.34TFMDD77 pKa = 4.61FFDD80 pKa = 4.29MLDD83 pKa = 3.79DD84 pKa = 4.59QAEE87 pKa = 4.0QGNNIVVNWRR97 pKa = 11.84YY98 pKa = 10.0HH99 pKa = 5.48EE100 pKa = 4.58EE101 pKa = 3.99NEE103 pKa = 4.34SAMEE107 pKa = 4.31CGEE110 pKa = 4.02EE111 pKa = 4.02FMEE114 pKa = 5.46DD115 pKa = 3.42VSCIKK120 pKa = 10.63FNLVEE125 pKa = 4.81FNDD128 pKa = 3.79EE129 pKa = 4.47SEE131 pKa = 4.38CC132 pKa = 4.72

Molecular weight: 15.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1WL06|M1WL06_PSEP2 Hydrogenase maturation factor OS=Pseudodesulfovibrio piezophilus (strain DSM 21447 / JCM 15486 / C1TLV30) OX=1322246 GN=hypD PE=3 SV=1
MM1 pKa = 7.7PKK3 pKa = 9.95IKK5 pKa = 9.47TRR7 pKa = 11.84RR8 pKa = 11.84AAAKK12 pKa = 9.74RR13 pKa = 11.84FSKK16 pKa = 9.7TATGKK21 pKa = 10.1FKK23 pKa = 10.74RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.07NLRR30 pKa = 11.84HH31 pKa = 6.24ILTKK35 pKa = 10.67KK36 pKa = 7.68NAKK39 pKa = 9.22RR40 pKa = 11.84KK41 pKa = 9.18RR42 pKa = 11.84RR43 pKa = 11.84LGQSTTVDD51 pKa = 3.67STNMKK56 pKa = 9.79AVRR59 pKa = 11.84RR60 pKa = 11.84QLPNGG65 pKa = 3.51

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3342

0

3342

1080842

20

2728

323.4

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.611 ± 0.049

1.316 ± 0.018

5.606 ± 0.031

6.71 ± 0.039

4.258 ± 0.028

7.75 ± 0.04

2.209 ± 0.02

6.155 ± 0.033

5.257 ± 0.042

10.211 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.021

3.376 ± 0.023

4.392 ± 0.031

3.352 ± 0.024

5.415 ± 0.034

6.043 ± 0.033

5.46 ± 0.036

7.015 ± 0.035

1.148 ± 0.017

2.721 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski