Amino acid dipepetide frequency for Synechococcus phage S-B64

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.387AlaAla: 6.387 ± 0.76
0.626AlaCys: 0.626 ± 0.143
4.119AlaAsp: 4.119 ± 0.371
4.588AlaGlu: 4.588 ± 0.416
2.972AlaPhe: 2.972 ± 0.332
6.699AlaGly: 6.699 ± 0.585
0.912AlaHis: 0.912 ± 0.162
4.04AlaIle: 4.04 ± 0.342
4.666AlaLys: 4.666 ± 0.493
5.213AlaLeu: 5.213 ± 0.469
1.434AlaMet: 1.434 ± 0.201
3.988AlaAsn: 3.988 ± 0.467
2.789AlaPro: 2.789 ± 0.311
2.737AlaGln: 2.737 ± 0.346
3.024AlaArg: 3.024 ± 0.326
4.849AlaSer: 4.849 ± 0.459
5.787AlaThr: 5.787 ± 0.69
4.145AlaVal: 4.145 ± 0.334
0.6AlaTrp: 0.6 ± 0.147
2.268AlaTyr: 2.268 ± 0.227
0.0AlaXaa: 0.0 ± 0.0
Cys
0.6CysAla: 0.6 ± 0.113
0.078CysCys: 0.078 ± 0.047
0.964CysAsp: 0.964 ± 0.159
0.678CysGlu: 0.678 ± 0.134
0.521CysPhe: 0.521 ± 0.129
0.652CysGly: 0.652 ± 0.16
0.261CysHis: 0.261 ± 0.08
0.6CysIle: 0.6 ± 0.147
0.678CysLys: 0.678 ± 0.144
0.521CysLeu: 0.521 ± 0.135
0.339CysMet: 0.339 ± 0.095
0.469CysAsn: 0.469 ± 0.118
0.365CysPro: 0.365 ± 0.093
0.339CysGln: 0.339 ± 0.088
0.469CysArg: 0.469 ± 0.121
0.547CysSer: 0.547 ± 0.133
0.365CysThr: 0.365 ± 0.092
0.469CysVal: 0.469 ± 0.108
0.104CysTrp: 0.104 ± 0.055
0.313CysTyr: 0.313 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
5.161AspAla: 5.161 ± 0.456
0.73AspCys: 0.73 ± 0.152
4.562AspAsp: 4.562 ± 0.366
4.431AspGlu: 4.431 ± 0.452
3.363AspPhe: 3.363 ± 0.336
5.839AspGly: 5.839 ± 0.598
1.121AspHis: 1.121 ± 0.185
3.258AspIle: 3.258 ± 0.293
3.702AspLys: 3.702 ± 0.383
5.396AspLeu: 5.396 ± 0.441
1.303AspMet: 1.303 ± 0.205
3.18AspAsn: 3.18 ± 0.342
3.519AspPro: 3.519 ± 0.357
2.19AspGln: 2.19 ± 0.218
2.763AspArg: 2.763 ± 0.234
3.649AspSer: 3.649 ± 0.319
4.04AspThr: 4.04 ± 0.324
4.562AspVal: 4.562 ± 0.392
1.017AspTrp: 1.017 ± 0.179
2.998AspTyr: 2.998 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
3.884GluAla: 3.884 ± 0.395
0.573GluCys: 0.573 ± 0.123
4.51GluAsp: 4.51 ± 0.433
6.074GluGlu: 6.074 ± 0.657
3.571GluPhe: 3.571 ± 0.34
4.145GluGly: 4.145 ± 0.405
1.017GluHis: 1.017 ± 0.162
4.64GluIle: 4.64 ± 0.415
4.822GluLys: 4.822 ± 0.559
4.484GluLeu: 4.484 ± 0.365
2.32GluMet: 2.32 ± 0.324
3.232GluAsn: 3.232 ± 0.3
1.356GluPro: 1.356 ± 0.201
2.294GluGln: 2.294 ± 0.252
2.841GluArg: 2.841 ± 0.34
3.91GluSer: 3.91 ± 0.393
3.962GluThr: 3.962 ± 0.452
4.64GluVal: 4.64 ± 0.339
0.886GluTrp: 0.886 ± 0.152
2.763GluTyr: 2.763 ± 0.288
0.0GluXaa: 0.0 ± 0.0
Phe
2.998PheAla: 2.998 ± 0.262
0.417PheCys: 0.417 ± 0.093
3.728PheAsp: 3.728 ± 0.283
2.607PheGlu: 2.607 ± 0.295
2.033PhePhe: 2.033 ± 0.231
2.476PheGly: 2.476 ± 0.284
0.886PheHis: 0.886 ± 0.208
2.737PheIle: 2.737 ± 0.322
2.685PheLys: 2.685 ± 0.295
3.102PheLeu: 3.102 ± 0.348
1.121PheMet: 1.121 ± 0.199
2.789PheAsn: 2.789 ± 0.257
1.825PhePro: 1.825 ± 0.21
1.434PheGln: 1.434 ± 0.207
1.668PheArg: 1.668 ± 0.218
3.285PheSer: 3.285 ± 0.338
3.128PheThr: 3.128 ± 0.297
2.633PheVal: 2.633 ± 0.33
0.652PheTrp: 0.652 ± 0.153
1.981PheTyr: 1.981 ± 0.217
0.0PheXaa: 0.0 ± 0.0
Gly
5.552GlyAla: 5.552 ± 0.585
0.678GlyCys: 0.678 ± 0.153
4.822GlyAsp: 4.822 ± 0.439
4.145GlyGlu: 4.145 ± 0.309
3.128GlyPhe: 3.128 ± 0.293
7.012GlyGly: 7.012 ± 0.984
0.991GlyHis: 0.991 ± 0.2
3.988GlyIle: 3.988 ± 0.385
3.78GlyLys: 3.78 ± 0.449
4.849GlyLeu: 4.849 ± 0.405
1.851GlyMet: 1.851 ± 0.231
4.405GlyAsn: 4.405 ± 0.509
1.955GlyPro: 1.955 ± 0.295
2.711GlyGln: 2.711 ± 0.317
3.285GlyArg: 3.285 ± 0.295
5.083GlySer: 5.083 ± 0.481
6.204GlyThr: 6.204 ± 0.744
4.822GlyVal: 4.822 ± 0.443
1.121GlyTrp: 1.121 ± 0.172
2.946GlyTyr: 2.946 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
0.652HisAla: 0.652 ± 0.119
0.261HisCys: 0.261 ± 0.073
0.912HisAsp: 0.912 ± 0.176
1.251HisGlu: 1.251 ± 0.239
0.886HisPhe: 0.886 ± 0.171
1.121HisGly: 1.121 ± 0.186
0.469HisHis: 0.469 ± 0.179
1.017HisIle: 1.017 ± 0.17
0.938HisLys: 0.938 ± 0.17
1.173HisLeu: 1.173 ± 0.196
0.391HisMet: 0.391 ± 0.12
0.73HisAsn: 0.73 ± 0.157
0.964HisPro: 0.964 ± 0.192
0.521HisGln: 0.521 ± 0.135
0.834HisArg: 0.834 ± 0.167
0.704HisSer: 0.704 ± 0.121
0.938HisThr: 0.938 ± 0.171
0.912HisVal: 0.912 ± 0.195
0.182HisTrp: 0.182 ± 0.081
0.834HisTyr: 0.834 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
3.936IleAla: 3.936 ± 0.354
0.495IleCys: 0.495 ± 0.11
4.822IleAsp: 4.822 ± 0.375
4.431IleGlu: 4.431 ± 0.323
2.45IlePhe: 2.45 ± 0.284
3.858IleGly: 3.858 ± 0.365
0.704IleHis: 0.704 ± 0.155
3.232IleIle: 3.232 ± 0.314
3.832IleLys: 3.832 ± 0.362
4.275IleLeu: 4.275 ± 0.366
0.86IleMet: 0.86 ± 0.165
3.91IleAsn: 3.91 ± 0.338
2.659IlePro: 2.659 ± 0.34
2.815IleGln: 2.815 ± 0.334
2.502IleArg: 2.502 ± 0.22
4.301IleSer: 4.301 ± 0.374
4.849IleThr: 4.849 ± 0.535
3.623IleVal: 3.623 ± 0.38
0.782IleTrp: 0.782 ± 0.183
2.502IleTyr: 2.502 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
3.754LysAla: 3.754 ± 0.513
0.886LysCys: 0.886 ± 0.183
3.728LysAsp: 3.728 ± 0.379
4.353LysGlu: 4.353 ± 0.566
2.737LysPhe: 2.737 ± 0.325
3.441LysGly: 3.441 ± 0.366
1.095LysHis: 1.095 ± 0.22
4.171LysIle: 4.171 ± 0.377
5.657LysLys: 5.657 ± 0.727
4.953LysLeu: 4.953 ± 0.503
1.694LysMet: 1.694 ± 0.259
3.05LysAsn: 3.05 ± 0.305
2.138LysPro: 2.138 ± 0.281
2.737LysGln: 2.737 ± 0.35
2.502LysArg: 2.502 ± 0.308
4.249LysSer: 4.249 ± 0.459
3.545LysThr: 3.545 ± 0.339
4.379LysVal: 4.379 ± 0.358
0.86LysTrp: 0.86 ± 0.181
3.467LysTyr: 3.467 ± 0.327
0.0LysXaa: 0.0 ± 0.0
Leu
5.631LeuAla: 5.631 ± 0.409
0.573LeuCys: 0.573 ± 0.129
5.292LeuAsp: 5.292 ± 0.347
4.744LeuGlu: 4.744 ± 0.393
2.815LeuPhe: 2.815 ± 0.319
4.405LeuGly: 4.405 ± 0.329
1.329LeuHis: 1.329 ± 0.205
4.301LeuIle: 4.301 ± 0.386
4.875LeuLys: 4.875 ± 0.509
5.318LeuLeu: 5.318 ± 0.531
1.199LeuMet: 1.199 ± 0.243
4.796LeuAsn: 4.796 ± 0.387
3.102LeuPro: 3.102 ± 0.285
2.841LeuGln: 2.841 ± 0.232
3.415LeuArg: 3.415 ± 0.314
4.849LeuSer: 4.849 ± 0.412
5.031LeuThr: 5.031 ± 0.393
4.796LeuVal: 4.796 ± 0.34
0.573LeuTrp: 0.573 ± 0.171
3.415LeuTyr: 3.415 ± 0.287
0.0LeuXaa: 0.0 ± 0.0
Met
1.642MetAla: 1.642 ± 0.259
0.235MetCys: 0.235 ± 0.085
1.017MetAsp: 1.017 ± 0.17
1.46MetGlu: 1.46 ± 0.224
0.73MetPhe: 0.73 ± 0.15
1.382MetGly: 1.382 ± 0.237
0.339MetHis: 0.339 ± 0.121
1.382MetIle: 1.382 ± 0.234
2.033MetLys: 2.033 ± 0.328
1.877MetLeu: 1.877 ± 0.238
0.443MetMet: 0.443 ± 0.11
1.225MetAsn: 1.225 ± 0.191
1.225MetPro: 1.225 ± 0.196
0.964MetGln: 0.964 ± 0.169
0.834MetArg: 0.834 ± 0.17
1.799MetSer: 1.799 ± 0.282
1.72MetThr: 1.72 ± 0.273
1.173MetVal: 1.173 ± 0.178
0.313MetTrp: 0.313 ± 0.084
0.756MetTyr: 0.756 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.285AsnAla: 3.285 ± 0.418
0.443AsnCys: 0.443 ± 0.108
3.232AsnAsp: 3.232 ± 0.314
3.363AsnGlu: 3.363 ± 0.254
2.607AsnPhe: 2.607 ± 0.239
4.51AsnGly: 4.51 ± 0.503
0.938AsnHis: 0.938 ± 0.16
4.014AsnIle: 4.014 ± 0.385
3.076AsnLys: 3.076 ± 0.367
4.536AsnLeu: 4.536 ± 0.43
1.017AsnMet: 1.017 ± 0.174
3.258AsnAsn: 3.258 ± 0.309
3.024AsnPro: 3.024 ± 0.299
2.268AsnGln: 2.268 ± 0.236
2.581AsnArg: 2.581 ± 0.207
3.649AsnSer: 3.649 ± 0.375
4.014AsnThr: 4.014 ± 0.637
4.014AsnVal: 4.014 ± 0.34
0.652AsnTrp: 0.652 ± 0.136
2.581AsnTyr: 2.581 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
3.076ProAla: 3.076 ± 0.314
0.313ProCys: 0.313 ± 0.088
2.633ProAsp: 2.633 ± 0.298
2.763ProGlu: 2.763 ± 0.286
1.903ProPhe: 1.903 ± 0.235
3.337ProGly: 3.337 ± 0.348
0.652ProHis: 0.652 ± 0.135
1.955ProIle: 1.955 ± 0.267
2.294ProLys: 2.294 ± 0.303
2.085ProLeu: 2.085 ± 0.245
0.73ProMet: 0.73 ± 0.147
2.32ProAsn: 2.32 ± 0.246
1.616ProPro: 1.616 ± 0.271
1.434ProGln: 1.434 ± 0.183
1.408ProArg: 1.408 ± 0.2
3.05ProSer: 3.05 ± 0.257
3.024ProThr: 3.024 ± 0.29
2.633ProVal: 2.633 ± 0.264
0.652ProTrp: 0.652 ± 0.133
1.747ProTyr: 1.747 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
2.502GlnAla: 2.502 ± 0.247
0.261GlnCys: 0.261 ± 0.082
2.372GlnAsp: 2.372 ± 0.209
2.45GlnGlu: 2.45 ± 0.286
1.564GlnPhe: 1.564 ± 0.23
2.242GlnGly: 2.242 ± 0.261
0.652GlnHis: 0.652 ± 0.168
2.92GlnIle: 2.92 ± 0.277
2.555GlnLys: 2.555 ± 0.385
3.18GlnLeu: 3.18 ± 0.343
0.912GlnMet: 0.912 ± 0.153
1.929GlnAsn: 1.929 ± 0.209
1.199GlnPro: 1.199 ± 0.175
1.434GlnGln: 1.434 ± 0.24
1.773GlnArg: 1.773 ± 0.224
2.164GlnSer: 2.164 ± 0.243
1.903GlnThr: 1.903 ± 0.235
2.867GlnVal: 2.867 ± 0.322
0.469GlnTrp: 0.469 ± 0.126
1.642GlnTyr: 1.642 ± 0.204
0.0GlnXaa: 0.0 ± 0.0
Arg
3.18ArgAla: 3.18 ± 0.327
0.417ArgCys: 0.417 ± 0.095
2.45ArgAsp: 2.45 ± 0.228
2.711ArgGlu: 2.711 ± 0.333
1.694ArgPhe: 1.694 ± 0.199
3.076ArgGly: 3.076 ± 0.281
0.704ArgHis: 0.704 ± 0.132
3.154ArgIle: 3.154 ± 0.357
2.815ArgLys: 2.815 ± 0.351
3.441ArgLeu: 3.441 ± 0.347
1.199ArgMet: 1.199 ± 0.195
2.085ArgAsn: 2.085 ± 0.245
1.356ArgPro: 1.356 ± 0.205
1.825ArgGln: 1.825 ± 0.21
2.32ArgArg: 2.32 ± 0.352
2.033ArgSer: 2.033 ± 0.218
2.398ArgThr: 2.398 ± 0.324
3.441ArgVal: 3.441 ± 0.304
0.443ArgTrp: 0.443 ± 0.118
2.19ArgTyr: 2.19 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
5.605SerAla: 5.605 ± 0.421
0.573SerCys: 0.573 ± 0.143
4.431SerAsp: 4.431 ± 0.366
3.936SerGlu: 3.936 ± 0.372
3.258SerPhe: 3.258 ± 0.268
5.552SerGly: 5.552 ± 0.508
1.017SerHis: 1.017 ± 0.194
3.754SerIle: 3.754 ± 0.512
3.597SerLys: 3.597 ± 0.304
4.431SerLeu: 4.431 ± 0.415
1.486SerMet: 1.486 ± 0.227
3.78SerAsn: 3.78 ± 0.401
2.45SerPro: 2.45 ± 0.318
2.085SerGln: 2.085 ± 0.256
2.372SerArg: 2.372 ± 0.211
4.145SerSer: 4.145 ± 0.453
4.484SerThr: 4.484 ± 0.502
4.666SerVal: 4.666 ± 0.388
0.626SerTrp: 0.626 ± 0.123
2.424SerTyr: 2.424 ± 0.274
0.0SerXaa: 0.0 ± 0.0
Thr
5.683ThrAla: 5.683 ± 0.581
0.443ThrCys: 0.443 ± 0.121
3.806ThrAsp: 3.806 ± 0.474
4.067ThrGlu: 4.067 ± 0.298
3.441ThrPhe: 3.441 ± 0.326
5.787ThrGly: 5.787 ± 0.689
0.912ThrHis: 0.912 ± 0.175
4.353ThrIle: 4.353 ± 0.394
3.441ThrLys: 3.441 ± 0.339
6.334ThrLeu: 6.334 ± 0.676
1.199ThrMet: 1.199 ± 0.201
3.91ThrAsn: 3.91 ± 0.561
3.441ThrPro: 3.441 ± 0.331
2.372ThrGln: 2.372 ± 0.23
2.476ThrArg: 2.476 ± 0.311
4.379ThrSer: 4.379 ± 0.489
5.318ThrThr: 5.318 ± 0.838
5.135ThrVal: 5.135 ± 0.602
0.6ThrTrp: 0.6 ± 0.122
2.633ThrTyr: 2.633 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
5.083ValAla: 5.083 ± 0.472
0.521ValCys: 0.521 ± 0.105
5.422ValAsp: 5.422 ± 0.539
4.458ValGlu: 4.458 ± 0.367
2.529ValPhe: 2.529 ± 0.24
4.562ValGly: 4.562 ± 0.467
0.652ValHis: 0.652 ± 0.144
3.858ValIle: 3.858 ± 0.373
4.484ValLys: 4.484 ± 0.398
4.145ValLeu: 4.145 ± 0.334
1.512ValMet: 1.512 ± 0.213
4.249ValAsn: 4.249 ± 0.36
2.45ValPro: 2.45 ± 0.249
2.059ValGln: 2.059 ± 0.194
3.024ValArg: 3.024 ± 0.317
4.77ValSer: 4.77 ± 0.353
5.657ValThr: 5.657 ± 0.54
4.536ValVal: 4.536 ± 0.386
0.756ValTrp: 0.756 ± 0.114
2.555ValTyr: 2.555 ± 0.29
0.0ValXaa: 0.0 ± 0.0
Trp
0.782TrpAla: 0.782 ± 0.118
0.104TrpCys: 0.104 ± 0.048
0.652TrpAsp: 0.652 ± 0.122
0.886TrpGlu: 0.886 ± 0.181
0.495TrpPhe: 0.495 ± 0.142
0.626TrpGly: 0.626 ± 0.131
0.365TrpHis: 0.365 ± 0.107
0.678TrpIle: 0.678 ± 0.096
0.782TrpLys: 0.782 ± 0.174
0.886TrpLeu: 0.886 ± 0.188
0.547TrpMet: 0.547 ± 0.137
0.834TrpAsn: 0.834 ± 0.156
0.339TrpPro: 0.339 ± 0.122
0.365TrpGln: 0.365 ± 0.093
0.521TrpArg: 0.521 ± 0.149
0.626TrpSer: 0.626 ± 0.14
0.756TrpThr: 0.756 ± 0.14
0.886TrpVal: 0.886 ± 0.172
0.235TrpTrp: 0.235 ± 0.084
0.678TrpTyr: 0.678 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.45TyrAla: 2.45 ± 0.225
0.704TyrCys: 0.704 ± 0.138
3.311TyrAsp: 3.311 ± 0.338
2.529TyrGlu: 2.529 ± 0.309
1.486TyrPhe: 1.486 ± 0.203
2.607TyrGly: 2.607 ± 0.276
0.678TyrHis: 0.678 ± 0.159
2.789TyrIle: 2.789 ± 0.258
2.685TyrLys: 2.685 ± 0.309
3.102TyrLeu: 3.102 ± 0.288
0.964TyrMet: 0.964 ± 0.185
2.92TyrAsn: 2.92 ± 0.283
1.851TyrPro: 1.851 ± 0.244
1.564TyrGln: 1.564 ± 0.193
2.32TyrArg: 2.32 ± 0.318
2.659TyrSer: 2.659 ± 0.267
2.607TyrThr: 2.607 ± 0.206
2.946TyrVal: 2.946 ± 0.293
0.521TyrTrp: 0.521 ± 0.128
1.799TyrTyr: 1.799 ± 0.229
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 156 proteins (38363 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski