Paenisporosarcina sp. K2R23-3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Paenisporosarcina; unclassified Paenisporosarcina

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2474 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385YU24|A0A385YU24_9BACL Pseudouridine synthase OS=Paenisporosarcina sp. K2R23-3 OX=2320858 GN=D3873_04520 PE=3 SV=1
MM1 pKa = 7.26TSNFIVQGDD10 pKa = 4.01SVYY13 pKa = 10.7KK14 pKa = 10.47SEE16 pKa = 5.37EE17 pKa = 4.08IVTDD21 pKa = 3.68TQTVSITSYY30 pKa = 10.64YY31 pKa = 10.77DD32 pKa = 3.03QATHH36 pKa = 6.57IRR38 pKa = 11.84LSTDD42 pKa = 2.57KK43 pKa = 10.44EE44 pKa = 4.71TILSNGTDD52 pKa = 3.37VATVTARR59 pKa = 11.84LYY61 pKa = 10.69NYY63 pKa = 8.66EE64 pKa = 3.68GQYY67 pKa = 10.52QVGSNDD73 pKa = 3.23SVTFSIDD80 pKa = 2.97GAEE83 pKa = 4.1QTLSLIDD90 pKa = 3.67GQVSIEE96 pKa = 4.14VTSDD100 pKa = 3.23VVDD103 pKa = 5.66DD104 pKa = 4.78ILITCHH110 pKa = 6.31APNVRR115 pKa = 11.84IGEE118 pKa = 4.32VVIRR122 pKa = 11.84AQTT125 pKa = 3.1

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385YW03|A0A385YW03_9BACL Elongation factor G-binding protein OS=Paenisporosarcina sp. K2R23-3 OX=2320858 GN=D3873_11735 PE=4 SV=1
MM1 pKa = 7.23LTGSVPMLRR10 pKa = 11.84KK11 pKa = 9.35SVPMLRR17 pKa = 11.84QSVPMLRR24 pKa = 11.84GSVPMKK30 pKa = 9.75RR31 pKa = 11.84QSVLMLTGSVPMLKK45 pKa = 10.03QSVPMKK51 pKa = 9.43RR52 pKa = 11.84QSVPMLRR59 pKa = 11.84QSVLMLKK66 pKa = 10.25QIVLMLTGSVPMVRR80 pKa = 11.84QSVRR84 pKa = 11.84MHH86 pKa = 6.33IKK88 pKa = 9.73VV89 pKa = 3.1

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2474

0

2474

715741

30

1492

289.3

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.429 ± 0.046

0.56 ± 0.014

5.176 ± 0.04

7.771 ± 0.056

4.602 ± 0.041

6.637 ± 0.051

2.147 ± 0.028

7.368 ± 0.044

6.372 ± 0.047

9.658 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.918 ± 0.024

3.999 ± 0.04

3.568 ± 0.025

3.846 ± 0.038

4.2 ± 0.037

6.313 ± 0.038

5.8 ± 0.035

7.349 ± 0.046

1.001 ± 0.016

3.284 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski