Amino acid dipepetide frequency for Paenisporosarcina sp. K2R23-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.945AlaAla: 5.945 ± 0.121	0.5AlaCys: 0.5 ± 0.026	3.607AlaAsp: 3.607 ± 0.088	4.919AlaGlu: 4.919 ± 0.1	3.504AlaPhe: 3.504 ± 0.092	5.122AlaGly: 5.122 ± 0.106	1.425AlaHis: 1.425 ± 0.046	6.507AlaIle: 6.507 ± 0.112	5.086AlaLys: 5.086 ± 0.095	7.293AlaLeu: 7.293 ± 0.12	2.29AlaMet: 2.29 ± 0.063	3.116AlaAsn: 3.116 ± 0.063	2.153AlaPro: 2.153 ± 0.065	2.4AlaGln: 2.4 ± 0.057	2.86AlaArg: 2.86 ± 0.074	4.785AlaSer: 4.785 ± 0.082	4.306AlaThr: 4.306 ± 0.081	5.371AlaVal: 5.371 ± 0.099	0.654AlaTrp: 0.654 ± 0.034	2.446AlaTyr: 2.446 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.373CysAla: 0.373 ± 0.025	0.064CysCys: 0.064 ± 0.01	0.328CysAsp: 0.328 ± 0.022	0.377CysGlu: 0.377 ± 0.023	0.218CysPhe: 0.218 ± 0.017	0.546CysGly: 0.546 ± 0.027	0.173CysHis: 0.173 ± 0.018	0.39CysIle: 0.39 ± 0.021	0.257CysLys: 0.257 ± 0.02	0.495CysLeu: 0.495 ± 0.028	0.149CysMet: 0.149 ± 0.013	0.214CysAsn: 0.214 ± 0.018	0.279CysPro: 0.279 ± 0.019	0.191CysGln: 0.191 ± 0.019	0.245CysArg: 0.245 ± 0.018	0.342CysSer: 0.342 ± 0.024	0.356CysThr: 0.356 ± 0.023	0.362CysVal: 0.362 ± 0.026	0.061CysTrp: 0.061 ± 0.008	0.177CysTyr: 0.177 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.057AspAla: 4.057 ± 0.091	0.313AspCys: 0.313 ± 0.019	2.481AspAsp: 2.481 ± 0.069	4.485AspGlu: 4.485 ± 0.089	2.537AspPhe: 2.537 ± 0.061	3.626AspGly: 3.626 ± 0.084	1.168AspHis: 1.168 ± 0.037	3.844AspIle: 3.844 ± 0.083	2.571AspLys: 2.571 ± 0.069	4.844AspLeu: 4.844 ± 0.083	1.445AspMet: 1.445 ± 0.045	1.621AspAsn: 1.621 ± 0.05	1.939AspPro: 1.939 ± 0.063	2.069AspGln: 2.069 ± 0.058	2.365AspArg: 2.365 ± 0.057	2.864AspSer: 2.864 ± 0.065	2.632AspThr: 2.632 ± 0.058	4.326AspVal: 4.326 ± 0.079	0.585AspTrp: 0.585 ± 0.03	1.992AspTyr: 1.992 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	5.658GluAla: 5.658 ± 0.113	0.305GluCys: 0.305 ± 0.024	3.916GluAsp: 3.916 ± 0.092	7.478GluGlu: 7.478 ± 0.15	2.708GluPhe: 2.708 ± 0.062	4.186GluGly: 4.186 ± 0.085	1.549GluHis: 1.549 ± 0.044	5.436GluIle: 5.436 ± 0.088	6.632GluLys: 6.632 ± 0.092	7.506GluLeu: 7.506 ± 0.113	2.789GluMet: 2.789 ± 0.071	3.751GluAsn: 3.751 ± 0.076	1.879GluPro: 1.879 ± 0.056	3.357GluGln: 3.357 ± 0.078	3.638GluArg: 3.638 ± 0.072	4.069GluSer: 4.069 ± 0.083	4.472GluThr: 4.472 ± 0.085	5.404GluVal: 5.404 ± 0.093	0.988GluTrp: 0.988 ± 0.043	1.992GluTyr: 1.992 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	3.335PheAla: 3.335 ± 0.081	0.25PheCys: 0.25 ± 0.019	2.59PheAsp: 2.59 ± 0.061	3.053PheGlu: 3.053 ± 0.064	2.466PhePhe: 2.466 ± 0.079	3.514PheGly: 3.514 ± 0.076	1.13PheHis: 1.13 ± 0.041	3.522PheIle: 3.522 ± 0.085	1.814PheLys: 1.814 ± 0.046	4.644PheLeu: 4.644 ± 0.114	1.213PheMet: 1.213 ± 0.042	1.492PheAsn: 1.492 ± 0.05	1.745PhePro: 1.745 ± 0.05	1.994PheGln: 1.994 ± 0.058	1.737PheArg: 1.737 ± 0.049	3.201PheSer: 3.201 ± 0.08	2.72PheThr: 2.72 ± 0.072	3.573PheVal: 3.573 ± 0.083	0.482PheTrp: 0.482 ± 0.023	1.549PheTyr: 1.549 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.84GlyAla: 4.84 ± 0.103	0.443GlyCys: 0.443 ± 0.027	3.345GlyAsp: 3.345 ± 0.076	4.482GlyGlu: 4.482 ± 0.075	3.352GlyPhe: 3.352 ± 0.071	4.372GlyGly: 4.372 ± 0.101	1.385GlyHis: 1.385 ± 0.062	5.649GlyIle: 5.649 ± 0.094	4.984GlyLys: 4.984 ± 0.084	5.888GlyLeu: 5.888 ± 0.104	2.206GlyMet: 2.206 ± 0.054	2.779GlyAsn: 2.779 ± 0.072	1.651GlyPro: 1.651 ± 0.056	2.209GlyGln: 2.209 ± 0.082	2.599GlyArg: 2.599 ± 0.067	3.862GlySer: 3.862 ± 0.078	4.031GlyThr: 4.031 ± 0.08	4.901GlyVal: 4.901 ± 0.094	0.693GlyTrp: 0.693 ± 0.033	2.701GlyTyr: 2.701 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.642HisAla: 1.642 ± 0.052	0.173HisCys: 0.173 ± 0.017	1.102HisAsp: 1.102 ± 0.038	1.442HisGlu: 1.442 ± 0.045	1.183HisPhe: 1.183 ± 0.051	1.404HisGly: 1.404 ± 0.048	0.69HisHis: 0.69 ± 0.029	1.336HisIle: 1.336 ± 0.045	0.851HisLys: 0.851 ± 0.032	2.325HisLeu: 2.325 ± 0.062	0.55HisMet: 0.55 ± 0.023	0.618HisAsn: 0.618 ± 0.03	1.192HisPro: 1.192 ± 0.039	0.903HisGln: 0.903 ± 0.034	0.951HisArg: 0.951 ± 0.035	1.308HisSer: 1.308 ± 0.045	1.081HisThr: 1.081 ± 0.038	1.714HisVal: 1.714 ± 0.049	0.196HisTrp: 0.196 ± 0.018	0.806HisTyr: 0.806 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.378IleAla: 6.378 ± 0.101	0.465IleCys: 0.465 ± 0.029	4.305IleAsp: 4.305 ± 0.088	5.552IleGlu: 5.552 ± 0.092	3.089IlePhe: 3.089 ± 0.083	5.991IleGly: 5.991 ± 0.108	1.758IleHis: 1.758 ± 0.04	5.009IleIle: 5.009 ± 0.094	3.18IleLys: 3.18 ± 0.063	6.607IleLeu: 6.607 ± 0.115	1.679IleMet: 1.679 ± 0.046	2.322IleAsn: 2.322 ± 0.069	3.335IlePro: 3.335 ± 0.072	3.236IleGln: 3.236 ± 0.07	3.369IleArg: 3.369 ± 0.082	4.858IleSer: 4.858 ± 0.079	3.99IleThr: 3.99 ± 0.076	5.878IleVal: 5.878 ± 0.103	0.599IleTrp: 0.599 ± 0.034	2.085IleTyr: 2.085 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.464LysAla: 4.464 ± 0.081	0.286LysCys: 0.286 ± 0.021	3.285LysAsp: 3.285 ± 0.069	6.602LysGlu: 6.602 ± 0.116	1.946LysPhe: 1.946 ± 0.053	3.955LysGly: 3.955 ± 0.081	1.175LysHis: 1.175 ± 0.038	3.953LysIle: 3.953 ± 0.068	5.225LysLys: 5.225 ± 0.097	5.473LysLeu: 5.473 ± 0.096	2.314LysMet: 2.314 ± 0.065	2.766LysAsn: 2.766 ± 0.077	1.978LysPro: 1.978 ± 0.058	2.745LysGln: 2.745 ± 0.068	3.209LysArg: 3.209 ± 0.073	3.669LysSer: 3.669 ± 0.071	3.665LysThr: 3.665 ± 0.065	4.373LysVal: 4.373 ± 0.079	0.89LysTrp: 0.89 ± 0.035	1.748LysTyr: 1.748 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	7.64LeuAla: 7.64 ± 0.117	0.462LeuCys: 0.462 ± 0.026	4.794LeuAsp: 4.794 ± 0.081	7.056LeuGlu: 7.056 ± 0.106	4.843LeuPhe: 4.843 ± 0.111	5.99LeuGly: 5.99 ± 0.102	2.279LeuHis: 2.279 ± 0.064	6.167LeuIle: 6.167 ± 0.11	5.631LeuLys: 5.631 ± 0.109	10.459LeuLeu: 10.459 ± 0.187	2.504LeuMet: 2.504 ± 0.062	3.45LeuAsn: 3.45 ± 0.061	3.954LeuPro: 3.954 ± 0.078	4.222LeuGln: 4.222 ± 0.081	3.983LeuArg: 3.983 ± 0.085	6.976LeuSer: 6.976 ± 0.103	5.64LeuThr: 5.64 ± 0.101	6.866LeuVal: 6.866 ± 0.109	0.781LeuTrp: 0.781 ± 0.037	2.887LeuTyr: 2.887 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.036MetAla: 2.036 ± 0.057	0.122MetCys: 0.122 ± 0.013	1.663MetAsp: 1.663 ± 0.055	2.465MetGlu: 2.465 ± 0.062	1.13MetPhe: 1.13 ± 0.044	1.667MetGly: 1.667 ± 0.059	0.532MetHis: 0.532 ± 0.028	2.072MetIle: 2.072 ± 0.05	2.597MetLys: 2.597 ± 0.057	2.425MetLeu: 2.425 ± 0.054	0.946MetMet: 0.946 ± 0.039	1.586MetAsn: 1.586 ± 0.048	1.013MetPro: 1.013 ± 0.036	1.048MetGln: 1.048 ± 0.04	1.186MetArg: 1.186 ± 0.041	1.82MetSer: 1.82 ± 0.064	2.034MetThr: 2.034 ± 0.049	1.781MetVal: 1.781 ± 0.05	0.215MetTrp: 0.215 ± 0.017	0.844MetTyr: 0.844 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.941AsnAla: 2.941 ± 0.075	0.219AsnCys: 0.219 ± 0.016	2.153AsnAsp: 2.153 ± 0.056	3.62AsnGlu: 3.62 ± 0.085	1.598AsnPhe: 1.598 ± 0.047	3.22AsnGly: 3.22 ± 0.072	0.835AsnHis: 0.835 ± 0.037	2.495AsnIle: 2.495 ± 0.069	2.357AsnLys: 2.357 ± 0.06	3.376AsnLeu: 3.376 ± 0.07	1.094AsnMet: 1.094 ± 0.035	1.477AsnAsn: 1.477 ± 0.046	1.895AsnPro: 1.895 ± 0.05	1.475AsnGln: 1.475 ± 0.047	2.132AsnArg: 2.132 ± 0.064	2.072AsnSer: 2.072 ± 0.062	1.995AsnThr: 1.995 ± 0.058	3.104AsnVal: 3.104 ± 0.073	0.496AsnTrp: 0.496 ± 0.028	1.433AsnTyr: 1.433 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.347ProAla: 2.347 ± 0.059	0.182ProCys: 0.182 ± 0.015	1.827ProAsp: 1.827 ± 0.051	2.942ProGlu: 2.942 ± 0.06	1.988ProPhe: 1.988 ± 0.054	2.132ProGly: 2.132 ± 0.058	0.759ProHis: 0.759 ± 0.034	2.956ProIle: 2.956 ± 0.069	2.167ProLys: 2.167 ± 0.063	3.366ProLeu: 3.366 ± 0.072	0.898ProMet: 0.898 ± 0.04	1.559ProAsn: 1.559 ± 0.05	0.965ProPro: 0.965 ± 0.052	1.044ProGln: 1.044 ± 0.041	1.02ProArg: 1.02 ± 0.043	2.617ProSer: 2.617 ± 0.073	2.441ProThr: 2.441 ± 0.068	2.84ProVal: 2.84 ± 0.065	0.34ProTrp: 0.34 ± 0.026	1.291ProTyr: 1.291 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.092GlnAla: 3.092 ± 0.064	0.137GlnCys: 0.137 ± 0.012	1.793GlnAsp: 1.793 ± 0.041	3.134GlnGlu: 3.134 ± 0.065	1.72GlnPhe: 1.72 ± 0.044	2.174GlnGly: 2.174 ± 0.086	0.898GlnHis: 0.898 ± 0.038	2.296GlnIle: 2.296 ± 0.055	2.562GlnLys: 2.562 ± 0.059	4.447GlnLeu: 4.447 ± 0.093	1.165GlnMet: 1.165 ± 0.038	1.499GlnAsn: 1.499 ± 0.047	1.277GlnPro: 1.277 ± 0.05	1.913GlnGln: 1.913 ± 0.064	1.509GlnArg: 1.509 ± 0.055	2.438GlnSer: 2.438 ± 0.062	2.284GlnThr: 2.284 ± 0.056	2.645GlnVal: 2.645 ± 0.061	0.349GlnTrp: 0.349 ± 0.024	1.132GlnTyr: 1.132 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.887ArgAla: 2.887 ± 0.069	0.196ArgCys: 0.196 ± 0.017	2.323ArgAsp: 2.323 ± 0.06	3.455ArgGlu: 3.455 ± 0.081	1.909ArgPhe: 1.909 ± 0.053	2.479ArgGly: 2.479 ± 0.07	0.88ArgHis: 0.88 ± 0.037	3.145ArgIle: 3.145 ± 0.065	3.19ArgLys: 3.19 ± 0.066	4.159ArgLeu: 4.159 ± 0.081	1.357ArgMet: 1.357 ± 0.042	1.811ArgAsn: 1.811 ± 0.054	1.372ArgPro: 1.372 ± 0.049	1.709ArgGln: 1.709 ± 0.05	1.82ArgArg: 1.82 ± 0.059	2.308ArgSer: 2.308 ± 0.054	2.14ArgThr: 2.14 ± 0.059	2.979ArgVal: 2.979 ± 0.067	0.404ArgTrp: 0.404 ± 0.026	1.478ArgTyr: 1.478 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	4.124SerAla: 4.124 ± 0.075	0.348SerCys: 0.348 ± 0.024	3.025SerAsp: 3.025 ± 0.066	4.224SerGlu: 4.224 ± 0.095	3.426SerPhe: 3.426 ± 0.083	4.284SerGly: 4.284 ± 0.085	1.257SerHis: 1.257 ± 0.048	5.533SerIle: 5.533 ± 0.097	3.873SerLys: 3.873 ± 0.08	6.117SerLeu: 6.117 ± 0.1	1.882SerMet: 1.882 ± 0.053	2.583SerAsn: 2.583 ± 0.054	2.326SerPro: 2.326 ± 0.063	1.991SerGln: 1.991 ± 0.053	2.333SerArg: 2.333 ± 0.06	4.464SerSer: 4.464 ± 0.102	3.788SerThr: 3.788 ± 0.088	4.576SerVal: 4.576 ± 0.086	0.574SerTrp: 0.574 ± 0.027	2.399SerTyr: 2.399 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	4.123ThrAla: 4.123 ± 0.072	0.334ThrCys: 0.334 ± 0.022	2.881ThrAsp: 2.881 ± 0.072	3.852ThrGlu: 3.852 ± 0.087	2.97ThrPhe: 2.97 ± 0.066	4.029ThrGly: 4.029 ± 0.085	1.19ThrHis: 1.19 ± 0.038	4.985ThrIle: 4.985 ± 0.082	3.663ThrLys: 3.663 ± 0.077	5.562ThrLeu: 5.562 ± 0.095	1.46ThrMet: 1.46 ± 0.041	2.541ThrAsn: 2.541 ± 0.062	2.423ThrPro: 2.423 ± 0.063	1.523ThrGln: 1.523 ± 0.047	2.068ThrArg: 2.068 ± 0.054	3.943ThrSer: 3.943 ± 0.078	3.557ThrThr: 3.557 ± 0.082	4.18ThrVal: 4.18 ± 0.091	0.552ThrTrp: 0.552 ± 0.031	2.164ThrTyr: 2.164 ± 0.065	0.0ThrXaa: 0.0 ± 0.0
Val	5.482ValAla: 5.482 ± 0.098	0.488ValCys: 0.488 ± 0.028	3.891ValAsp: 3.891 ± 0.075	5.463ValGlu: 5.463 ± 0.088	3.268ValPhe: 3.268 ± 0.079	4.788ValGly: 4.788 ± 0.088	1.474ValHis: 1.474 ± 0.045	5.438ValIle: 5.438 ± 0.08	4.622ValLys: 4.622 ± 0.08	7.275ValLeu: 7.275 ± 0.107	2.009ValMet: 2.009 ± 0.059	2.954ValAsn: 2.954 ± 0.073	2.72ValPro: 2.72 ± 0.06	2.727ValGln: 2.727 ± 0.056	3.022ValArg: 3.022 ± 0.063	4.919ValSer: 4.919 ± 0.085	4.516ValThr: 4.516 ± 0.106	5.617ValVal: 5.617 ± 0.108	0.637ValTrp: 0.637 ± 0.03	2.185ValTyr: 2.185 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.542TrpAla: 0.542 ± 0.031	0.071TrpCys: 0.071 ± 0.01	0.486TrpAsp: 0.486 ± 0.025	0.679TrpGlu: 0.679 ± 0.034	0.443TrpPhe: 0.443 ± 0.026	0.563TrpGly: 0.563 ± 0.029	0.207TrpHis: 0.207 ± 0.016	0.822TrpIle: 0.822 ± 0.036	0.903TrpLys: 0.903 ± 0.035	1.002TrpLeu: 1.002 ± 0.042	0.393TrpMet: 0.393 ± 0.022	0.616TrpAsn: 0.616 ± 0.031	0.221TrpPro: 0.221 ± 0.02	0.388TrpGln: 0.388 ± 0.024	0.418TrpArg: 0.418 ± 0.027	0.647TrpSer: 0.647 ± 0.03	0.583TrpThr: 0.583 ± 0.026	0.599TrpVal: 0.599 ± 0.031	0.127TrpTrp: 0.127 ± 0.015	0.298TrpTyr: 0.298 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.384TyrAla: 2.384 ± 0.067	0.242TyrCys: 0.242 ± 0.021	1.969TyrAsp: 1.969 ± 0.079	2.435TyrGlu: 2.435 ± 0.062	1.725TyrPhe: 1.725 ± 0.049	2.328TyrGly: 2.328 ± 0.053	0.701TyrHis: 0.701 ± 0.031	2.132TyrIle: 2.132 ± 0.061	1.558TyrLys: 1.558 ± 0.049	3.32TyrLeu: 3.32 ± 0.075	0.838TyrMet: 0.838 ± 0.032	1.235TyrAsn: 1.235 ± 0.043	1.364TyrPro: 1.364 ± 0.05	1.319TyrGln: 1.319 ± 0.045	1.535TyrArg: 1.535 ± 0.047	1.966TyrSer: 1.966 ± 0.056	1.769TyrThr: 1.769 ± 0.058	2.406TyrVal: 2.406 ± 0.062	0.383TyrTrp: 0.383 ± 0.023	1.231TyrTyr: 1.231 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2474 proteins (715741 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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