Pseudomonas phage vB_PaeM_USP_25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5U151|A0A7D5U151_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_USP_25 OX=2743939 GN=vBPaeMUSP25_55 PE=4 SV=1
MM1 pKa = 7.97SDD3 pKa = 3.27TQEE6 pKa = 3.95KK7 pKa = 8.99ATIEE11 pKa = 4.23RR12 pKa = 11.84DD13 pKa = 3.25WKK15 pKa = 9.77PSAEE19 pKa = 3.54FRR21 pKa = 11.84YY22 pKa = 9.93FIFDD26 pKa = 3.66PEE28 pKa = 4.07QGEE31 pKa = 4.13FVYY34 pKa = 10.43FRR36 pKa = 11.84TAEE39 pKa = 4.26DD40 pKa = 3.89RR41 pKa = 11.84DD42 pKa = 3.61QSADD46 pKa = 4.04GIIQSYY52 pKa = 10.74LDD54 pKa = 4.73DD55 pKa = 4.3GWDD58 pKa = 3.36EE59 pKa = 3.88AVEE62 pKa = 4.2NIVAGEE68 pKa = 4.17VTHH71 pKa = 6.8TCDD74 pKa = 3.52KK75 pKa = 11.21VNVQEE80 pKa = 5.07RR81 pKa = 11.84PPEE84 pKa = 4.08DD85 pKa = 4.26EE86 pKa = 4.76IDD88 pKa = 3.95GEE90 pKa = 5.67GYY92 pKa = 10.61DD93 pKa = 4.32QDD95 pKa = 3.55GNYY98 pKa = 8.44WAEE101 pKa = 3.87EE102 pKa = 3.45WSYY105 pKa = 11.08RR106 pKa = 11.84CGYY109 pKa = 9.5EE110 pKa = 4.28LIAVAPEE117 pKa = 3.85AATQSDD123 pKa = 4.57GEE125 pKa = 4.35QPP127 pKa = 3.24

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5Q4C7|A0A7D5Q4C7_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_USP_25 OX=2743939 GN=vBPaeMUSP25_30B PE=4 SV=1
MM1 pKa = 6.37TQPRR5 pKa = 11.84IDD7 pKa = 4.73SIRR10 pKa = 11.84MTYY13 pKa = 10.37RR14 pKa = 11.84SDD16 pKa = 3.52PPRR19 pKa = 11.84KK20 pKa = 9.39KK21 pKa = 10.1PQAGDD26 pKa = 3.13IRR28 pKa = 11.84ITGGKK33 pKa = 7.81TMIRR37 pKa = 11.84QQVKK41 pKa = 8.77HH42 pKa = 6.22NGMYY46 pKa = 9.77VVSGGRR52 pKa = 11.84PVWEE56 pKa = 3.94WVEE59 pKa = 4.06KK60 pKa = 10.91GSAEE64 pKa = 4.43DD65 pKa = 3.42RR66 pKa = 11.84TAKK69 pKa = 10.29RR70 pKa = 3.5

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

19620

42

4899

297.3

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.314 ± 0.538

0.79 ± 0.235

6.157 ± 0.302

6.896 ± 0.368

2.961 ± 0.146

7.783 ± 0.425

2.003 ± 0.205

4.521 ± 0.191

4.424 ± 0.31

7.477 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.206

3.053 ± 0.178

5.413 ± 0.433

4.924 ± 0.284

7.12 ± 0.237

5.433 ± 0.228

5.53 ± 0.208

6.488 ± 0.246

1.498 ± 0.179

2.63 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski