Amino acid dipepetide frequency for Pseudomonas phage vB_PaeM_USP_25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.629AlaAla: 14.629 ± 1.469
0.765AlaCys: 0.765 ± 0.274
8.155AlaAsp: 8.155 ± 0.741
9.735AlaGlu: 9.735 ± 1.214
3.415AlaPhe: 3.415 ± 0.352
9.124AlaGly: 9.124 ± 0.976
2.447AlaHis: 2.447 ± 0.334
5.097AlaIle: 5.097 ± 0.538
6.269AlaLys: 6.269 ± 0.848
9.124AlaLeu: 9.124 ± 0.667
3.721AlaMet: 3.721 ± 0.467
3.823AlaAsn: 3.823 ± 0.426
6.524AlaPro: 6.524 ± 0.711
6.728AlaGln: 6.728 ± 0.651
8.206AlaArg: 8.206 ± 0.618
6.728AlaSer: 6.728 ± 0.671
6.83AlaThr: 6.83 ± 0.778
8.053AlaVal: 8.053 ± 0.539
1.427AlaTrp: 1.427 ± 0.261
2.345AlaTyr: 2.345 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
0.765CysAla: 0.765 ± 0.26
0.255CysCys: 0.255 ± 0.13
0.459CysAsp: 0.459 ± 0.187
0.357CysGlu: 0.357 ± 0.168
0.051CysPhe: 0.051 ± 0.06
1.121CysGly: 1.121 ± 0.372
0.255CysHis: 0.255 ± 0.139
0.357CysIle: 0.357 ± 0.234
0.612CysLys: 0.612 ± 0.27
0.714CysLeu: 0.714 ± 0.269
0.408CysMet: 0.408 ± 0.163
0.408CysAsn: 0.408 ± 0.21
0.255CysPro: 0.255 ± 0.124
0.306CysGln: 0.306 ± 0.158
0.255CysArg: 0.255 ± 0.152
0.561CysSer: 0.561 ± 0.191
0.204CysThr: 0.204 ± 0.116
0.306CysVal: 0.306 ± 0.16
0.102CysTrp: 0.102 ± 0.093
0.153CysTyr: 0.153 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
7.544AspAla: 7.544 ± 0.967
0.459AspCys: 0.459 ± 0.19
3.67AspAsp: 3.67 ± 0.478
3.568AspGlu: 3.568 ± 0.428
1.733AspPhe: 1.733 ± 0.303
5.964AspGly: 5.964 ± 0.623
1.019AspHis: 1.019 ± 0.244
3.262AspIle: 3.262 ± 0.383
2.956AspLys: 2.956 ± 0.466
4.791AspLeu: 4.791 ± 0.368
1.733AspMet: 1.733 ± 0.351
1.019AspAsn: 1.019 ± 0.216
3.313AspPro: 3.313 ± 0.478
3.211AspGln: 3.211 ± 0.365
3.517AspArg: 3.517 ± 0.391
3.568AspSer: 3.568 ± 0.4
3.313AspThr: 3.313 ± 0.5
3.568AspVal: 3.568 ± 0.486
1.529AspTrp: 1.529 ± 0.308
1.835AspTyr: 1.835 ± 0.467
0.0AspXaa: 0.0 ± 0.0
Glu
9.634GluAla: 9.634 ± 0.931
0.357GluCys: 0.357 ± 0.181
3.874GluAsp: 3.874 ± 0.458
3.211GluGlu: 3.211 ± 0.54
2.294GluPhe: 2.294 ± 0.257
5.301GluGly: 5.301 ± 0.478
0.968GluHis: 0.968 ± 0.223
3.16GluIle: 3.16 ± 0.361
2.6GluLys: 2.6 ± 0.535
6.677GluLeu: 6.677 ± 0.623
1.784GluMet: 1.784 ± 0.287
1.886GluAsn: 1.886 ± 0.323
3.007GluPro: 3.007 ± 0.76
4.027GluGln: 4.027 ± 0.478
6.066GluArg: 6.066 ± 0.7
3.925GluSer: 3.925 ± 0.321
3.109GluThr: 3.109 ± 0.483
3.619GluVal: 3.619 ± 0.555
1.019GluTrp: 1.019 ± 0.207
2.447GluTyr: 2.447 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
3.619PheAla: 3.619 ± 0.415
0.051PheCys: 0.051 ± 0.053
1.886PheAsp: 1.886 ± 0.41
1.733PheGlu: 1.733 ± 0.41
0.867PhePhe: 0.867 ± 0.196
3.109PheGly: 3.109 ± 0.545
0.714PheHis: 0.714 ± 0.233
1.121PheIle: 1.121 ± 0.189
1.529PheLys: 1.529 ± 0.291
2.039PheLeu: 2.039 ± 0.286
0.561PheMet: 0.561 ± 0.164
1.121PheAsn: 1.121 ± 0.202
1.682PhePro: 1.682 ± 0.363
1.274PheGln: 1.274 ± 0.259
1.58PheArg: 1.58 ± 0.302
2.09PheSer: 2.09 ± 0.299
1.835PheThr: 1.835 ± 0.299
1.631PheVal: 1.631 ± 0.314
0.408PheTrp: 0.408 ± 0.136
0.765PheTyr: 0.765 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
8.206GlyAla: 8.206 ± 0.692
0.816GlyCys: 0.816 ± 0.315
5.862GlyAsp: 5.862 ± 0.846
5.811GlyGlu: 5.811 ± 0.571
3.058GlyPhe: 3.058 ± 0.791
5.76GlyGly: 5.76 ± 0.708
1.121GlyHis: 1.121 ± 0.201
4.587GlyIle: 4.587 ± 0.579
2.956GlyLys: 2.956 ± 0.546
4.536GlyLeu: 4.536 ± 0.556
2.09GlyMet: 2.09 ± 0.379
2.345GlyAsn: 2.345 ± 0.449
2.549GlyPro: 2.549 ± 0.364
3.415GlyGln: 3.415 ± 0.404
5.352GlyArg: 5.352 ± 0.619
4.231GlySer: 4.231 ± 0.391
4.995GlyThr: 4.995 ± 0.596
5.862GlyVal: 5.862 ± 0.603
1.427GlyTrp: 1.427 ± 0.303
2.854GlyTyr: 2.854 ± 0.608
0.0GlyXaa: 0.0 ± 0.0
His
1.784HisAla: 1.784 ± 0.409
0.255HisCys: 0.255 ± 0.129
1.427HisAsp: 1.427 ± 0.21
1.019HisGlu: 1.019 ± 0.243
0.663HisPhe: 0.663 ± 0.279
1.733HisGly: 1.733 ± 0.299
0.459HisHis: 0.459 ± 0.135
0.714HisIle: 0.714 ± 0.214
0.765HisLys: 0.765 ± 0.195
1.223HisLeu: 1.223 ± 0.236
0.306HisMet: 0.306 ± 0.119
0.663HisAsn: 0.663 ± 0.199
1.427HisPro: 1.427 ± 0.354
1.121HisGln: 1.121 ± 0.194
2.141HisArg: 2.141 ± 0.382
0.867HisSer: 0.867 ± 0.227
1.019HisThr: 1.019 ± 0.163
1.172HisVal: 1.172 ± 0.266
0.459HisTrp: 0.459 ± 0.164
0.816HisTyr: 0.816 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
6.32IleAla: 6.32 ± 0.533
0.408IleCys: 0.408 ± 0.144
3.007IleAsp: 3.007 ± 0.376
4.078IleGlu: 4.078 ± 0.396
1.121IlePhe: 1.121 ± 0.223
3.568IleGly: 3.568 ± 0.347
0.968IleHis: 0.968 ± 0.21
1.529IleIle: 1.529 ± 0.245
1.937IleLys: 1.937 ± 0.301
2.396IleLeu: 2.396 ± 0.353
0.663IleMet: 0.663 ± 0.164
1.631IleAsn: 1.631 ± 0.405
3.058IlePro: 3.058 ± 0.473
1.886IleGln: 1.886 ± 0.354
3.109IleArg: 3.109 ± 0.475
2.6IleSer: 2.6 ± 0.473
2.752IleThr: 2.752 ± 0.438
3.058IleVal: 3.058 ± 0.41
0.408IleTrp: 0.408 ± 0.135
0.714IleTyr: 0.714 ± 0.203
0.0IleXaa: 0.0 ± 0.0
Lys
6.167LysAla: 6.167 ± 0.725
0.357LysCys: 0.357 ± 0.153
2.192LysAsp: 2.192 ± 0.38
2.803LysGlu: 2.803 ± 0.445
0.917LysPhe: 0.917 ± 0.256
3.415LysGly: 3.415 ± 0.402
0.765LysHis: 0.765 ± 0.25
1.784LysIle: 1.784 ± 0.439
2.345LysLys: 2.345 ± 0.353
3.619LysLeu: 3.619 ± 0.319
0.968LysMet: 0.968 ± 0.221
1.172LysAsn: 1.172 ± 0.287
3.007LysPro: 3.007 ± 0.399
2.243LysGln: 2.243 ± 0.368
3.109LysArg: 3.109 ± 0.543
1.937LysSer: 1.937 ± 0.285
2.752LysThr: 2.752 ± 0.411
3.364LysVal: 3.364 ± 0.39
0.306LysTrp: 0.306 ± 0.106
1.019LysTyr: 1.019 ± 0.245
0.0LysXaa: 0.0 ± 0.0
Leu
10.245LeuAla: 10.245 ± 0.782
0.765LeuCys: 0.765 ± 0.27
4.485LeuAsp: 4.485 ± 0.467
5.301LeuGlu: 5.301 ± 0.405
2.243LeuPhe: 2.243 ± 0.277
4.893LeuGly: 4.893 ± 0.508
1.886LeuHis: 1.886 ± 0.288
3.415LeuIle: 3.415 ± 0.528
2.905LeuLys: 2.905 ± 0.38
4.842LeuLeu: 4.842 ± 0.459
1.733LeuMet: 1.733 ± 0.296
2.345LeuAsn: 2.345 ± 0.376
4.485LeuPro: 4.485 ± 0.684
2.294LeuGln: 2.294 ± 0.327
6.422LeuArg: 6.422 ± 0.413
4.587LeuSer: 4.587 ± 0.418
4.485LeuThr: 4.485 ± 0.463
5.097LeuVal: 5.097 ± 0.633
0.917LeuTrp: 0.917 ± 0.181
1.427LeuTyr: 1.427 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
3.007MetAla: 3.007 ± 0.385
0.051MetCys: 0.051 ± 0.052
1.58MetAsp: 1.58 ± 0.341
0.917MetGlu: 0.917 ± 0.221
0.204MetPhe: 0.204 ± 0.145
2.039MetGly: 2.039 ± 0.371
0.917MetHis: 0.917 ± 0.241
0.917MetIle: 0.917 ± 0.217
1.682MetLys: 1.682 ± 0.396
1.58MetLeu: 1.58 ± 0.286
0.459MetMet: 0.459 ± 0.188
0.612MetAsn: 0.612 ± 0.137
2.141MetPro: 2.141 ± 0.309
1.121MetGln: 1.121 ± 0.236
2.345MetArg: 2.345 ± 0.556
1.58MetSer: 1.58 ± 0.268
2.09MetThr: 2.09 ± 0.341
1.631MetVal: 1.631 ± 0.39
0.459MetTrp: 0.459 ± 0.151
0.51MetTyr: 0.51 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.67AsnAla: 3.67 ± 0.456
0.204AsnCys: 0.204 ± 0.118
1.937AsnAsp: 1.937 ± 0.304
2.141AsnGlu: 2.141 ± 0.266
0.816AsnPhe: 0.816 ± 0.187
2.549AsnGly: 2.549 ± 0.364
0.204AsnHis: 0.204 ± 0.12
0.968AsnIle: 0.968 ± 0.193
1.274AsnLys: 1.274 ± 0.196
2.345AsnLeu: 2.345 ± 0.307
0.867AsnMet: 0.867 ± 0.212
1.121AsnAsn: 1.121 ± 0.327
1.631AsnPro: 1.631 ± 0.322
1.784AsnGln: 1.784 ± 0.294
1.835AsnArg: 1.835 ± 0.286
1.784AsnSer: 1.784 ± 0.374
1.937AsnThr: 1.937 ± 0.293
1.58AsnVal: 1.58 ± 0.287
0.561AsnTrp: 0.561 ± 0.17
1.325AsnTyr: 1.325 ± 0.298
0.0AsnXaa: 0.0 ± 0.0
Pro
6.728ProAla: 6.728 ± 0.623
0.459ProCys: 0.459 ± 0.244
3.058ProAsp: 3.058 ± 0.578
4.842ProGlu: 4.842 ± 0.436
1.58ProPhe: 1.58 ± 0.273
3.364ProGly: 3.364 ± 0.378
0.917ProHis: 0.917 ± 0.229
2.498ProIle: 2.498 ± 0.277
2.294ProLys: 2.294 ± 0.367
3.313ProLeu: 3.313 ± 0.562
1.325ProMet: 1.325 ± 0.274
1.682ProAsn: 1.682 ± 0.307
3.313ProPro: 3.313 ± 0.948
3.109ProGln: 3.109 ± 0.359
2.549ProArg: 2.549 ± 0.488
3.823ProSer: 3.823 ± 0.78
2.854ProThr: 2.854 ± 0.467
4.129ProVal: 4.129 ± 0.655
0.51ProTrp: 0.51 ± 0.241
1.784ProTyr: 1.784 ± 0.37
0.0ProXaa: 0.0 ± 0.0
Gln
7.391GlnAla: 7.391 ± 0.991
0.153GlnCys: 0.153 ± 0.113
2.141GlnAsp: 2.141 ± 0.272
3.619GlnGlu: 3.619 ± 0.512
1.529GlnPhe: 1.529 ± 0.255
3.466GlnGly: 3.466 ± 0.412
1.376GlnHis: 1.376 ± 0.235
2.243GlnIle: 2.243 ± 0.383
1.223GlnLys: 1.223 ± 0.251
4.18GlnLeu: 4.18 ± 0.45
1.529GlnMet: 1.529 ± 0.39
1.376GlnAsn: 1.376 ± 0.36
2.65GlnPro: 2.65 ± 0.516
3.313GlnGln: 3.313 ± 0.533
3.823GlnArg: 3.823 ± 0.397
2.447GlnSer: 2.447 ± 0.296
1.886GlnThr: 1.886 ± 0.304
2.956GlnVal: 2.956 ± 0.304
0.612GlnTrp: 0.612 ± 0.151
1.325GlnTyr: 1.325 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
8.41ArgAla: 8.41 ± 0.715
0.714ArgCys: 0.714 ± 0.267
4.231ArgAsp: 4.231 ± 0.539
5.352ArgGlu: 5.352 ± 0.741
2.243ArgPhe: 2.243 ± 0.419
3.721ArgGly: 3.721 ± 0.491
1.631ArgHis: 1.631 ± 0.322
3.721ArgIle: 3.721 ± 0.331
3.466ArgLys: 3.466 ± 0.453
6.575ArgLeu: 6.575 ± 0.531
1.886ArgMet: 1.886 ± 0.268
2.6ArgAsn: 2.6 ± 0.455
3.364ArgPro: 3.364 ± 0.55
3.976ArgGln: 3.976 ± 0.535
4.995ArgArg: 4.995 ± 0.477
3.364ArgSer: 3.364 ± 0.536
3.109ArgThr: 3.109 ± 0.427
4.231ArgVal: 4.231 ± 0.48
1.019ArgTrp: 1.019 ± 0.351
2.549ArgTyr: 2.549 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
5.556SerAla: 5.556 ± 0.505
0.357SerCys: 0.357 ± 0.158
3.772SerAsp: 3.772 ± 0.426
3.721SerGlu: 3.721 ± 0.403
2.141SerPhe: 2.141 ± 0.334
6.167SerGly: 6.167 ± 0.637
1.427SerHis: 1.427 ± 0.336
2.396SerIle: 2.396 ± 0.29
2.854SerLys: 2.854 ± 0.386
4.333SerLeu: 4.333 ± 0.425
1.274SerMet: 1.274 ± 0.199
1.529SerAsn: 1.529 ± 0.246
2.854SerPro: 2.854 ± 0.633
2.345SerGln: 2.345 ± 0.344
2.701SerArg: 2.701 ± 0.296
2.141SerSer: 2.141 ± 0.384
3.109SerThr: 3.109 ± 0.376
3.466SerVal: 3.466 ± 0.415
1.07SerTrp: 1.07 ± 0.279
1.121SerTyr: 1.121 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
6.677ThrAla: 6.677 ± 0.643
0.561ThrCys: 0.561 ± 0.248
3.568ThrAsp: 3.568 ± 0.514
3.364ThrGlu: 3.364 ± 0.416
1.784ThrPhe: 1.784 ± 0.309
5.352ThrGly: 5.352 ± 0.638
0.968ThrHis: 0.968 ± 0.221
2.294ThrIle: 2.294 ± 0.355
2.294ThrLys: 2.294 ± 0.257
3.925ThrLeu: 3.925 ± 0.424
1.325ThrMet: 1.325 ± 0.259
1.478ThrAsn: 1.478 ± 0.179
4.027ThrPro: 4.027 ± 0.461
2.141ThrGln: 2.141 ± 0.327
4.078ThrArg: 4.078 ± 0.376
2.549ThrSer: 2.549 ± 0.412
2.803ThrThr: 2.803 ± 0.404
3.925ThrVal: 3.925 ± 0.47
1.274ThrTrp: 1.274 ± 0.243
0.917ThrTyr: 0.917 ± 0.175
0.0ThrXaa: 0.0 ± 0.0
Val
8.563ValAla: 8.563 ± 0.663
0.561ValCys: 0.561 ± 0.234
3.262ValAsp: 3.262 ± 0.437
5.301ValGlu: 5.301 ± 0.625
1.682ValPhe: 1.682 ± 0.285
4.129ValGly: 4.129 ± 0.588
0.765ValHis: 0.765 ± 0.142
3.466ValIle: 3.466 ± 0.323
2.701ValLys: 2.701 ± 0.379
4.434ValLeu: 4.434 ± 0.514
1.937ValMet: 1.937 ± 0.334
2.447ValAsn: 2.447 ± 0.519
3.313ValPro: 3.313 ± 0.383
2.905ValGln: 2.905 ± 0.421
5.301ValArg: 5.301 ± 0.565
3.466ValSer: 3.466 ± 0.367
4.282ValThr: 4.282 ± 0.512
4.231ValVal: 4.231 ± 0.533
0.612ValTrp: 0.612 ± 0.172
1.529ValTyr: 1.529 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
1.121TrpAla: 1.121 ± 0.297
0.102TrpCys: 0.102 ± 0.095
1.121TrpAsp: 1.121 ± 0.173
0.765TrpGlu: 0.765 ± 0.181
0.255TrpPhe: 0.255 ± 0.105
1.121TrpGly: 1.121 ± 0.322
0.459TrpHis: 0.459 ± 0.17
0.51TrpIle: 0.51 ± 0.204
0.663TrpLys: 0.663 ± 0.177
1.427TrpLeu: 1.427 ± 0.275
0.459TrpMet: 0.459 ± 0.131
0.561TrpAsn: 0.561 ± 0.123
0.612TrpPro: 0.612 ± 0.172
0.816TrpGln: 0.816 ± 0.252
1.427TrpArg: 1.427 ± 0.289
1.019TrpSer: 1.019 ± 0.268
0.816TrpThr: 0.816 ± 0.232
1.121TrpVal: 1.121 ± 0.258
0.306TrpTrp: 0.306 ± 0.128
0.306TrpTyr: 0.306 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.109TyrAla: 3.109 ± 0.363
0.255TyrCys: 0.255 ± 0.154
1.886TyrAsp: 1.886 ± 0.281
1.325TyrGlu: 1.325 ± 0.266
1.019TyrPhe: 1.019 ± 0.284
1.937TyrGly: 1.937 ± 0.53
0.765TyrHis: 0.765 ± 0.195
1.172TyrIle: 1.172 ± 0.198
0.917TyrLys: 0.917 ± 0.186
2.701TyrLeu: 2.701 ± 0.538
0.765TyrMet: 0.765 ± 0.186
0.714TyrAsn: 0.714 ± 0.224
0.917TyrPro: 0.917 ± 0.162
1.223TyrGln: 1.223 ± 0.27
2.396TyrArg: 2.396 ± 0.441
1.07TyrSer: 1.07 ± 0.217
1.121TyrThr: 1.121 ± 0.339
1.886TyrVal: 1.886 ± 0.462
0.561TyrTrp: 0.561 ± 0.206
0.561TyrTyr: 0.561 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (19620 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski