Amino acid dipepetide frequency for Burkholderia phage KL3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.415AlaAla: 18.415 ± 2.408
0.787AlaCys: 0.787 ± 0.371
8.421AlaAsp: 8.421 ± 0.934
5.981AlaGlu: 5.981 ± 0.537
3.699AlaPhe: 3.699 ± 0.429
9.601AlaGly: 9.601 ± 0.82
1.889AlaHis: 1.889 ± 0.367
4.171AlaIle: 4.171 ± 0.516
4.958AlaLys: 4.958 ± 0.667
12.749AlaLeu: 12.749 ± 1.41
3.777AlaMet: 3.777 ± 0.43
3.384AlaAsn: 3.384 ± 0.477
5.273AlaPro: 5.273 ± 0.644
3.62AlaGln: 3.62 ± 0.74
10.467AlaArg: 10.467 ± 0.992
5.981AlaSer: 5.981 ± 0.921
7.161AlaThr: 7.161 ± 0.771
7.397AlaVal: 7.397 ± 0.791
2.676AlaTrp: 2.676 ± 0.486
3.305AlaTyr: 3.305 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.866CysAla: 0.866 ± 0.298
0.079CysCys: 0.079 ± 0.061
0.315CysAsp: 0.315 ± 0.157
0.393CysGlu: 0.393 ± 0.163
0.472CysPhe: 0.472 ± 0.176
0.866CysGly: 0.866 ± 0.271
0.157CysHis: 0.157 ± 0.13
0.315CysIle: 0.315 ± 0.165
0.393CysLys: 0.393 ± 0.149
0.315CysLeu: 0.315 ± 0.166
0.236CysMet: 0.236 ± 0.127
0.393CysAsn: 0.393 ± 0.216
0.551CysPro: 0.551 ± 0.183
0.393CysGln: 0.393 ± 0.177
0.551CysArg: 0.551 ± 0.231
0.079CysSer: 0.079 ± 0.065
0.393CysThr: 0.393 ± 0.164
0.551CysVal: 0.551 ± 0.213
0.236CysTrp: 0.236 ± 0.135
0.236CysTyr: 0.236 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
8.499AspAla: 8.499 ± 0.941
0.393AspCys: 0.393 ± 0.168
5.037AspAsp: 5.037 ± 0.658
3.935AspGlu: 3.935 ± 0.635
2.597AspPhe: 2.597 ± 0.418
7.397AspGly: 7.397 ± 1.029
1.574AspHis: 1.574 ± 0.372
2.676AspIle: 2.676 ± 0.505
3.148AspLys: 3.148 ± 0.572
5.43AspLeu: 5.43 ± 0.859
1.653AspMet: 1.653 ± 0.409
1.18AspAsn: 1.18 ± 0.284
3.305AspPro: 3.305 ± 0.462
2.597AspGln: 2.597 ± 0.503
3.384AspArg: 3.384 ± 0.664
1.967AspSer: 1.967 ± 0.423
3.856AspThr: 3.856 ± 0.631
4.722AspVal: 4.722 ± 0.61
0.787AspTrp: 0.787 ± 0.292
1.889AspTyr: 1.889 ± 0.414
0.0AspXaa: 0.0 ± 0.0
Glu
4.171GluAla: 4.171 ± 0.608
1.023GluCys: 1.023 ± 0.334
1.653GluAsp: 1.653 ± 0.392
1.889GluGlu: 1.889 ± 0.452
2.125GluPhe: 2.125 ± 0.392
3.541GluGly: 3.541 ± 0.493
0.551GluHis: 0.551 ± 0.205
3.227GluIle: 3.227 ± 0.489
2.204GluLys: 2.204 ± 0.441
5.666GluLeu: 5.666 ± 0.605
1.417GluMet: 1.417 ± 0.341
2.361GluAsn: 2.361 ± 0.621
3.069GluPro: 3.069 ± 0.5
2.282GluGln: 2.282 ± 0.418
5.115GluArg: 5.115 ± 0.732
2.912GluSer: 2.912 ± 0.469
3.227GluThr: 3.227 ± 0.591
2.204GluVal: 2.204 ± 0.413
1.102GluTrp: 1.102 ± 0.28
1.731GluTyr: 1.731 ± 0.44
0.0GluXaa: 0.0 ± 0.0
Phe
4.958PheAla: 4.958 ± 0.533
0.079PheCys: 0.079 ± 0.061
2.754PheAsp: 2.754 ± 0.5
2.125PheGlu: 2.125 ± 0.352
1.417PhePhe: 1.417 ± 0.364
3.227PheGly: 3.227 ± 0.57
0.315PheHis: 0.315 ± 0.145
1.81PheIle: 1.81 ± 0.44
1.417PheLys: 1.417 ± 0.269
2.125PheLeu: 2.125 ± 0.37
0.472PheMet: 0.472 ± 0.147
0.787PheAsn: 0.787 ± 0.215
1.574PhePro: 1.574 ± 0.445
1.18PheGln: 1.18 ± 0.331
2.912PheArg: 2.912 ± 0.564
2.597PheSer: 2.597 ± 0.471
2.282PheThr: 2.282 ± 0.427
1.889PheVal: 1.889 ± 0.365
0.787PheTrp: 0.787 ± 0.234
0.866PheTyr: 0.866 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
8.735GlyAla: 8.735 ± 1.024
0.708GlyCys: 0.708 ± 0.218
5.273GlyAsp: 5.273 ± 0.659
3.777GlyGlu: 3.777 ± 0.466
4.25GlyPhe: 4.25 ± 0.804
8.106GlyGly: 8.106 ± 1.144
1.731GlyHis: 1.731 ± 0.414
3.463GlyIle: 3.463 ± 0.557
4.171GlyLys: 4.171 ± 0.666
6.374GlyLeu: 6.374 ± 1.019
2.518GlyMet: 2.518 ± 0.415
2.518GlyAsn: 2.518 ± 0.796
3.541GlyPro: 3.541 ± 0.549
1.731GlyGln: 1.731 ± 0.368
6.611GlyArg: 6.611 ± 0.676
3.541GlySer: 3.541 ± 0.594
5.902GlyThr: 5.902 ± 0.642
5.037GlyVal: 5.037 ± 0.687
1.81GlyTrp: 1.81 ± 0.357
2.046GlyTyr: 2.046 ± 0.413
0.0GlyXaa: 0.0 ± 0.0
His
2.518HisAla: 2.518 ± 0.404
0.157HisCys: 0.157 ± 0.112
1.417HisAsp: 1.417 ± 0.416
1.259HisGlu: 1.259 ± 0.332
0.315HisPhe: 0.315 ± 0.155
2.282HisGly: 2.282 ± 0.427
0.708HisHis: 0.708 ± 0.196
0.944HisIle: 0.944 ± 0.259
1.102HisLys: 1.102 ± 0.333
1.18HisLeu: 1.18 ± 0.326
0.157HisMet: 0.157 ± 0.132
0.551HisAsn: 0.551 ± 0.2
1.102HisPro: 1.102 ± 0.307
0.63HisGln: 0.63 ± 0.191
1.023HisArg: 1.023 ± 0.307
1.18HisSer: 1.18 ± 0.344
1.102HisThr: 1.102 ± 0.273
1.81HisVal: 1.81 ± 0.373
0.236HisTrp: 0.236 ± 0.141
0.708HisTyr: 0.708 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
6.217IleAla: 6.217 ± 0.607
0.157IleCys: 0.157 ± 0.1
4.879IleAsp: 4.879 ± 0.546
2.99IleGlu: 2.99 ± 0.553
1.102IlePhe: 1.102 ± 0.253
4.171IleGly: 4.171 ± 0.765
0.63IleHis: 0.63 ± 0.201
0.944IleIle: 0.944 ± 0.256
1.81IleLys: 1.81 ± 0.458
3.069IleLeu: 3.069 ± 0.421
0.866IleMet: 0.866 ± 0.223
1.731IleAsn: 1.731 ± 0.361
1.495IlePro: 1.495 ± 0.292
1.495IleGln: 1.495 ± 0.363
2.361IleArg: 2.361 ± 0.441
2.676IleSer: 2.676 ± 0.472
2.44IleThr: 2.44 ± 0.445
4.25IleVal: 4.25 ± 0.414
0.315IleTrp: 0.315 ± 0.133
0.63IleTyr: 0.63 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
4.564LysAla: 4.564 ± 0.584
0.315LysCys: 0.315 ± 0.125
2.204LysAsp: 2.204 ± 0.414
1.574LysGlu: 1.574 ± 0.436
1.18LysPhe: 1.18 ± 0.281
3.699LysGly: 3.699 ± 0.491
1.18LysHis: 1.18 ± 0.306
1.259LysIle: 1.259 ± 0.391
1.967LysLys: 1.967 ± 0.408
3.384LysLeu: 3.384 ± 0.585
0.944LysMet: 0.944 ± 0.391
1.18LysAsn: 1.18 ± 0.26
3.148LysPro: 3.148 ± 0.379
1.889LysGln: 1.889 ± 0.351
4.25LysArg: 4.25 ± 0.544
1.889LysSer: 1.889 ± 0.422
2.676LysThr: 2.676 ± 0.443
2.361LysVal: 2.361 ± 0.491
0.472LysTrp: 0.472 ± 0.166
0.944LysTyr: 0.944 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
11.726LeuAla: 11.726 ± 1.148
0.944LeuCys: 0.944 ± 0.301
5.981LeuAsp: 5.981 ± 0.73
4.722LeuGlu: 4.722 ± 0.686
2.99LeuPhe: 2.99 ± 0.464
5.509LeuGly: 5.509 ± 1.096
2.204LeuHis: 2.204 ± 0.415
4.328LeuIle: 4.328 ± 0.828
2.912LeuLys: 2.912 ± 0.419
6.611LeuLeu: 6.611 ± 0.498
1.967LeuMet: 1.967 ± 0.386
3.541LeuAsn: 3.541 ± 0.539
4.25LeuPro: 4.25 ± 0.596
2.754LeuGln: 2.754 ± 0.362
7.24LeuArg: 7.24 ± 0.877
5.587LeuSer: 5.587 ± 0.556
6.217LeuThr: 6.217 ± 0.653
6.611LeuVal: 6.611 ± 0.642
0.63LeuTrp: 0.63 ± 0.201
2.361LeuTyr: 2.361 ± 0.492
0.0LeuXaa: 0.0 ± 0.0
Met
2.361MetAla: 2.361 ± 0.416
0.079MetCys: 0.079 ± 0.079
1.653MetAsp: 1.653 ± 0.479
1.023MetGlu: 1.023 ± 0.235
0.551MetPhe: 0.551 ± 0.262
1.731MetGly: 1.731 ± 0.392
0.472MetHis: 0.472 ± 0.196
0.944MetIle: 0.944 ± 0.276
1.259MetLys: 1.259 ± 0.268
2.282MetLeu: 2.282 ± 0.37
0.393MetMet: 0.393 ± 0.151
1.102MetAsn: 1.102 ± 0.316
1.259MetPro: 1.259 ± 0.314
0.708MetGln: 0.708 ± 0.363
1.967MetArg: 1.967 ± 0.471
1.102MetSer: 1.102 ± 0.345
2.282MetThr: 2.282 ± 0.321
1.338MetVal: 1.338 ± 0.344
0.079MetTrp: 0.079 ± 0.063
0.472MetTyr: 0.472 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.384AsnAla: 3.384 ± 0.446
0.236AsnCys: 0.236 ± 0.119
3.069AsnAsp: 3.069 ± 0.619
1.889AsnGlu: 1.889 ± 0.365
0.944AsnPhe: 0.944 ± 0.279
3.699AsnGly: 3.699 ± 0.514
0.708AsnHis: 0.708 ± 0.247
1.495AsnIle: 1.495 ± 0.321
1.023AsnLys: 1.023 ± 0.209
2.597AsnLeu: 2.597 ± 0.535
0.866AsnMet: 0.866 ± 0.387
0.866AsnAsn: 0.866 ± 0.243
1.417AsnPro: 1.417 ± 0.279
1.023AsnGln: 1.023 ± 0.228
1.889AsnArg: 1.889 ± 0.34
1.18AsnSer: 1.18 ± 0.31
1.81AsnThr: 1.81 ± 0.493
2.597AsnVal: 2.597 ± 0.465
0.393AsnTrp: 0.393 ± 0.195
0.787AsnTyr: 0.787 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
6.138ProAla: 6.138 ± 0.655
0.315ProCys: 0.315 ± 0.193
4.014ProAsp: 4.014 ± 0.586
2.754ProGlu: 2.754 ± 0.457
1.259ProPhe: 1.259 ± 0.372
3.069ProGly: 3.069 ± 0.65
0.866ProHis: 0.866 ± 0.263
3.069ProIle: 3.069 ± 0.401
1.81ProLys: 1.81 ± 0.383
3.463ProLeu: 3.463 ± 0.57
0.787ProMet: 0.787 ± 0.288
1.731ProAsn: 1.731 ± 0.375
2.754ProPro: 2.754 ± 0.587
1.338ProGln: 1.338 ± 0.291
3.384ProArg: 3.384 ± 0.505
2.99ProSer: 2.99 ± 0.378
2.597ProThr: 2.597 ± 0.46
3.541ProVal: 3.541 ± 0.575
0.63ProTrp: 0.63 ± 0.217
0.944ProTyr: 0.944 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
4.722GlnAla: 4.722 ± 0.584
0.236GlnCys: 0.236 ± 0.112
1.653GlnAsp: 1.653 ± 0.364
1.338GlnGlu: 1.338 ± 0.37
1.338GlnPhe: 1.338 ± 0.338
2.597GlnGly: 2.597 ± 0.416
0.708GlnHis: 0.708 ± 0.215
1.653GlnIle: 1.653 ± 0.588
1.495GlnLys: 1.495 ± 0.335
3.541GlnLeu: 3.541 ± 0.612
1.102GlnMet: 1.102 ± 0.43
0.63GlnAsn: 0.63 ± 0.199
1.259GlnPro: 1.259 ± 0.345
1.417GlnGln: 1.417 ± 0.35
3.227GlnArg: 3.227 ± 0.475
1.889GlnSer: 1.889 ± 0.352
2.282GlnThr: 2.282 ± 0.435
1.495GlnVal: 1.495 ± 0.304
0.708GlnTrp: 0.708 ± 0.201
1.259GlnTyr: 1.259 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
9.444ArgAla: 9.444 ± 0.957
0.63ArgCys: 0.63 ± 0.234
4.328ArgAsp: 4.328 ± 0.569
5.194ArgGlu: 5.194 ± 0.525
2.282ArgPhe: 2.282 ± 0.531
5.273ArgGly: 5.273 ± 0.835
1.495ArgHis: 1.495 ± 0.313
4.328ArgIle: 4.328 ± 0.661
3.305ArgLys: 3.305 ± 0.447
7.476ArgLeu: 7.476 ± 0.84
1.259ArgMet: 1.259 ± 0.265
2.912ArgAsn: 2.912 ± 0.564
2.518ArgPro: 2.518 ± 0.389
3.541ArgGln: 3.541 ± 0.523
6.138ArgArg: 6.138 ± 0.921
4.486ArgSer: 4.486 ± 0.64
4.092ArgThr: 4.092 ± 0.578
5.43ArgVal: 5.43 ± 0.502
1.18ArgTrp: 1.18 ± 0.261
2.44ArgTyr: 2.44 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
6.06SerAla: 6.06 ± 0.619
0.157SerCys: 0.157 ± 0.097
2.754SerAsp: 2.754 ± 0.45
2.361SerGlu: 2.361 ± 0.492
1.574SerPhe: 1.574 ± 0.395
4.486SerGly: 4.486 ± 0.526
1.023SerHis: 1.023 ± 0.263
3.227SerIle: 3.227 ± 0.481
1.653SerLys: 1.653 ± 0.354
5.43SerLeu: 5.43 ± 0.536
0.866SerMet: 0.866 ± 0.299
1.889SerAsn: 1.889 ± 0.515
2.676SerPro: 2.676 ± 0.372
1.731SerGln: 1.731 ± 0.421
4.171SerArg: 4.171 ± 0.457
2.99SerSer: 2.99 ± 0.655
4.328SerThr: 4.328 ± 0.614
3.384SerVal: 3.384 ± 0.422
0.551SerTrp: 0.551 ± 0.216
0.787SerTyr: 0.787 ± 0.208
0.0SerXaa: 0.0 ± 0.0
Thr
6.925ThrAla: 6.925 ± 0.685
0.551ThrCys: 0.551 ± 0.179
3.935ThrAsp: 3.935 ± 0.593
3.463ThrGlu: 3.463 ± 0.533
2.833ThrPhe: 2.833 ± 0.523
5.587ThrGly: 5.587 ± 0.788
1.338ThrHis: 1.338 ± 0.378
2.125ThrIle: 2.125 ± 0.387
2.597ThrLys: 2.597 ± 0.512
6.611ThrLeu: 6.611 ± 0.857
1.259ThrMet: 1.259 ± 0.289
2.046ThrAsn: 2.046 ± 0.372
4.25ThrPro: 4.25 ± 0.763
1.967ThrGln: 1.967 ± 0.385
3.699ThrArg: 3.699 ± 0.504
3.541ThrSer: 3.541 ± 0.502
3.935ThrThr: 3.935 ± 0.583
5.273ThrVal: 5.273 ± 0.7
0.708ThrTrp: 0.708 ± 0.204
1.102ThrTyr: 1.102 ± 0.366
0.0ThrXaa: 0.0 ± 0.0
Val
8.735ValAla: 8.735 ± 0.828
0.472ValCys: 0.472 ± 0.255
4.879ValAsp: 4.879 ± 0.642
3.069ValGlu: 3.069 ± 0.543
2.44ValPhe: 2.44 ± 0.419
5.037ValGly: 5.037 ± 0.628
0.944ValHis: 0.944 ± 0.265
3.148ValIle: 3.148 ± 0.492
2.833ValLys: 2.833 ± 0.323
5.587ValLeu: 5.587 ± 0.664
1.967ValMet: 1.967 ± 0.375
2.204ValAsn: 2.204 ± 0.438
2.754ValPro: 2.754 ± 0.544
2.125ValGln: 2.125 ± 0.456
5.666ValArg: 5.666 ± 0.58
3.777ValSer: 3.777 ± 0.718
4.722ValThr: 4.722 ± 0.725
5.115ValVal: 5.115 ± 0.52
1.023ValTrp: 1.023 ± 0.293
1.81ValTyr: 1.81 ± 0.328
0.0ValXaa: 0.0 ± 0.0
Trp
1.889TrpAla: 1.889 ± 0.295
0.157TrpCys: 0.157 ± 0.116
0.551TrpAsp: 0.551 ± 0.255
0.472TrpGlu: 0.472 ± 0.159
0.63TrpPhe: 0.63 ± 0.209
0.866TrpGly: 0.866 ± 0.418
0.708TrpHis: 0.708 ± 0.215
0.708TrpIle: 0.708 ± 0.21
0.393TrpLys: 0.393 ± 0.173
2.518TrpLeu: 2.518 ± 0.515
0.079TrpMet: 0.079 ± 0.077
0.315TrpAsn: 0.315 ± 0.141
0.472TrpPro: 0.472 ± 0.246
0.472TrpGln: 0.472 ± 0.204
1.889TrpArg: 1.889 ± 0.392
0.472TrpSer: 0.472 ± 0.141
1.023TrpThr: 1.023 ± 0.249
0.866TrpVal: 0.866 ± 0.297
0.079TrpTrp: 0.079 ± 0.071
0.315TrpTyr: 0.315 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.384TyrAla: 3.384 ± 0.556
0.315TyrCys: 0.315 ± 0.122
1.259TyrAsp: 1.259 ± 0.304
1.259TyrGlu: 1.259 ± 0.369
1.574TyrPhe: 1.574 ± 0.416
1.102TyrGly: 1.102 ± 0.272
1.18TyrHis: 1.18 ± 0.283
0.393TyrIle: 0.393 ± 0.203
0.63TyrLys: 0.63 ± 0.19
2.676TyrLeu: 2.676 ± 0.456
0.393TyrMet: 0.393 ± 0.164
0.551TyrAsn: 0.551 ± 0.156
0.787TyrPro: 0.787 ± 0.237
1.653TyrGln: 1.653 ± 0.276
1.889TyrArg: 1.889 ± 0.452
1.259TyrSer: 1.259 ± 0.301
1.417TyrThr: 1.417 ± 0.287
2.44TyrVal: 2.44 ± 0.449
0.472TyrTrp: 0.472 ± 0.139
0.472TyrTyr: 0.472 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski