Amino acid dipepetide frequency for Clostridium phage phiCD111

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.244AlaAla: 0.244 ± 0.174
0.488AlaCys: 0.488 ± 0.186
2.845AlaAsp: 2.845 ± 0.407
4.877AlaGlu: 4.877 ± 0.557
2.52AlaPhe: 2.52 ± 0.51
4.39AlaGly: 4.39 ± 0.746
0.325AlaHis: 0.325 ± 0.169
6.503AlaIle: 6.503 ± 0.883
4.877AlaLys: 4.877 ± 0.668
4.796AlaLeu: 4.796 ± 0.845
1.87AlaMet: 1.87 ± 0.471
3.333AlaAsn: 3.333 ± 0.456
0.975AlaPro: 0.975 ± 0.252
1.626AlaGln: 1.626 ± 0.358
3.333AlaArg: 3.333 ± 0.629
3.333AlaSer: 3.333 ± 0.46
4.308AlaThr: 4.308 ± 0.643
3.17AlaVal: 3.17 ± 0.65
0.406AlaTrp: 0.406 ± 0.143
1.87AlaTyr: 1.87 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.21
0.406CysCys: 0.406 ± 0.277
0.569CysAsp: 0.569 ± 0.236
1.382CysGlu: 1.382 ± 0.369
0.325CysPhe: 0.325 ± 0.155
0.732CysGly: 0.732 ± 0.247
0.081CysHis: 0.081 ± 0.09
0.65CysIle: 0.65 ± 0.301
0.813CysLys: 0.813 ± 0.235
0.894CysLeu: 0.894 ± 0.281
0.081CysMet: 0.081 ± 0.077
0.488CysAsn: 0.488 ± 0.205
0.244CysPro: 0.244 ± 0.138
0.163CysGln: 0.163 ± 0.102
0.244CysArg: 0.244 ± 0.149
1.057CysSer: 1.057 ± 0.368
0.325CysThr: 0.325 ± 0.132
0.406CysVal: 0.406 ± 0.163
0.081CysTrp: 0.081 ± 0.078
0.406CysTyr: 0.406 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
3.983AspAla: 3.983 ± 0.537
0.813AspCys: 0.813 ± 0.301
2.357AspAsp: 2.357 ± 0.421
3.333AspGlu: 3.333 ± 0.532
2.682AspPhe: 2.682 ± 0.539
3.821AspGly: 3.821 ± 0.619
0.163AspHis: 0.163 ± 0.12
7.235AspIle: 7.235 ± 0.801
5.446AspLys: 5.446 ± 0.655
5.934AspLeu: 5.934 ± 0.54
1.544AspMet: 1.544 ± 0.292
3.983AspAsn: 3.983 ± 0.609
0.894AspPro: 0.894 ± 0.311
0.488AspGln: 0.488 ± 0.163
2.195AspArg: 2.195 ± 0.356
4.552AspSer: 4.552 ± 0.529
2.439AspThr: 2.439 ± 0.427
3.414AspVal: 3.414 ± 0.433
0.569AspTrp: 0.569 ± 0.258
3.495AspTyr: 3.495 ± 0.645
0.0AspXaa: 0.0 ± 0.0
Glu
4.796GluAla: 4.796 ± 0.679
0.732GluCys: 0.732 ± 0.229
3.17GluAsp: 3.17 ± 0.425
8.454GluGlu: 8.454 ± 0.755
3.821GluPhe: 3.821 ± 0.782
4.715GluGly: 4.715 ± 0.611
0.894GluHis: 0.894 ± 0.259
7.722GluIle: 7.722 ± 1.13
9.267GluLys: 9.267 ± 1.085
8.535GluLeu: 8.535 ± 1.058
2.926GluMet: 2.926 ± 0.599
6.909GluAsn: 6.909 ± 0.918
0.65GluPro: 0.65 ± 0.257
3.252GluGln: 3.252 ± 0.497
2.357GluArg: 2.357 ± 0.526
4.796GluSer: 4.796 ± 0.839
5.284GluThr: 5.284 ± 0.594
5.365GluVal: 5.365 ± 0.803
0.813GluTrp: 0.813 ± 0.399
4.39GluTyr: 4.39 ± 0.818
0.0GluXaa: 0.0 ± 0.0
Phe
3.008PheAla: 3.008 ± 0.555
0.325PheCys: 0.325 ± 0.165
2.439PheAsp: 2.439 ± 0.36
2.926PheGlu: 2.926 ± 0.445
2.764PhePhe: 2.764 ± 0.783
2.439PheGly: 2.439 ± 0.756
0.244PheHis: 0.244 ± 0.172
3.252PheIle: 3.252 ± 0.478
4.39PheLys: 4.39 ± 0.594
2.926PheLeu: 2.926 ± 0.442
0.813PheMet: 0.813 ± 0.285
4.633PheAsn: 4.633 ± 0.672
0.569PhePro: 0.569 ± 0.209
0.732PheGln: 0.732 ± 0.197
1.788PheArg: 1.788 ± 0.386
3.577PheSer: 3.577 ± 0.532
2.601PheThr: 2.601 ± 0.355
1.87PheVal: 1.87 ± 0.453
0.081PheTrp: 0.081 ± 0.09
2.113PheTyr: 2.113 ± 0.511
0.0PheXaa: 0.0 ± 0.0
Gly
4.39GlyAla: 4.39 ± 0.639
0.163GlyCys: 0.163 ± 0.107
4.715GlyAsp: 4.715 ± 0.557
4.471GlyGlu: 4.471 ± 0.581
3.252GlyPhe: 3.252 ± 1.058
3.658GlyGly: 3.658 ± 0.738
0.65GlyHis: 0.65 ± 0.252
5.934GlyIle: 5.934 ± 0.755
5.69GlyLys: 5.69 ± 0.616
5.121GlyLeu: 5.121 ± 1.04
0.813GlyMet: 0.813 ± 0.2
3.495GlyAsn: 3.495 ± 0.739
0.081GlyPro: 0.081 ± 0.065
2.113GlyGln: 2.113 ± 0.594
1.788GlyArg: 1.788 ± 0.334
4.064GlySer: 4.064 ± 0.567
2.926GlyThr: 2.926 ± 0.548
3.008GlyVal: 3.008 ± 0.538
0.732GlyTrp: 0.732 ± 0.27
2.276GlyTyr: 2.276 ± 0.506
0.0GlyXaa: 0.0 ± 0.0
His
0.569HisAla: 0.569 ± 0.248
0.163HisCys: 0.163 ± 0.105
0.325HisAsp: 0.325 ± 0.195
0.894HisGlu: 0.894 ± 0.279
0.325HisPhe: 0.325 ± 0.156
0.732HisGly: 0.732 ± 0.199
0.081HisHis: 0.081 ± 0.09
0.325HisIle: 0.325 ± 0.158
0.975HisLys: 0.975 ± 0.317
0.65HisLeu: 0.65 ± 0.2
0.081HisMet: 0.081 ± 0.066
0.488HisAsn: 0.488 ± 0.193
0.0HisPro: 0.0 ± 0.0
0.406HisGln: 0.406 ± 0.187
0.163HisArg: 0.163 ± 0.134
1.138HisSer: 1.138 ± 0.285
0.569HisThr: 0.569 ± 0.361
0.488HisVal: 0.488 ± 0.204
0.244HisTrp: 0.244 ± 0.129
0.569HisTyr: 0.569 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.715IleAla: 4.715 ± 0.625
0.813IleCys: 0.813 ± 0.243
6.259IleAsp: 6.259 ± 0.622
9.917IleGlu: 9.917 ± 1.353
3.089IlePhe: 3.089 ± 0.577
5.284IleGly: 5.284 ± 1.253
1.219IleHis: 1.219 ± 0.312
6.097IleIle: 6.097 ± 0.667
11.299IleLys: 11.299 ± 1.047
5.365IleLeu: 5.365 ± 1.018
2.195IleMet: 2.195 ± 0.399
7.153IleAsn: 7.153 ± 0.767
2.52IlePro: 2.52 ± 0.427
2.926IleGln: 2.926 ± 0.579
3.577IleArg: 3.577 ± 0.625
5.69IleSer: 5.69 ± 0.67
3.739IleThr: 3.739 ± 0.43
5.04IleVal: 5.04 ± 0.583
0.569IleTrp: 0.569 ± 0.171
3.17IleTyr: 3.17 ± 0.444
0.0IleXaa: 0.0 ± 0.0
Lys
5.446LysAla: 5.446 ± 0.732
0.894LysCys: 0.894 ± 0.256
6.666LysAsp: 6.666 ± 0.665
11.624LysGlu: 11.624 ± 1.478
4.471LysPhe: 4.471 ± 0.535
5.284LysGly: 5.284 ± 0.876
0.569LysHis: 0.569 ± 0.301
9.185LysIle: 9.185 ± 0.778
9.673LysLys: 9.673 ± 1.152
7.478LysLeu: 7.478 ± 1.088
2.195LysMet: 2.195 ± 0.532
8.129LysAsn: 8.129 ± 0.812
0.894LysPro: 0.894 ± 0.339
2.926LysGln: 2.926 ± 0.463
2.52LysArg: 2.52 ± 0.507
6.666LysSer: 6.666 ± 0.539
4.552LysThr: 4.552 ± 0.621
6.178LysVal: 6.178 ± 0.479
0.975LysTrp: 0.975 ± 0.281
4.552LysTyr: 4.552 ± 0.724
0.0LysXaa: 0.0 ± 0.0
Leu
3.983LeuAla: 3.983 ± 0.597
0.813LeuCys: 0.813 ± 0.279
5.365LeuAsp: 5.365 ± 0.682
7.56LeuGlu: 7.56 ± 0.889
3.17LeuPhe: 3.17 ± 0.409
5.284LeuGly: 5.284 ± 0.629
0.406LeuHis: 0.406 ± 0.215
7.153LeuIle: 7.153 ± 0.751
9.023LeuLys: 9.023 ± 0.608
6.34LeuLeu: 6.34 ± 0.787
1.463LeuMet: 1.463 ± 0.456
6.828LeuAsn: 6.828 ± 0.8
2.357LeuPro: 2.357 ± 0.532
2.357LeuGln: 2.357 ± 0.51
2.195LeuArg: 2.195 ± 0.425
4.064LeuSer: 4.064 ± 0.69
4.064LeuThr: 4.064 ± 0.546
3.739LeuVal: 3.739 ± 0.597
0.65LeuTrp: 0.65 ± 0.277
2.682LeuTyr: 2.682 ± 0.548
0.0LeuXaa: 0.0 ± 0.0
Met
1.057MetAla: 1.057 ± 0.261
0.163MetCys: 0.163 ± 0.105
1.138MetAsp: 1.138 ± 0.32
1.626MetGlu: 1.626 ± 0.39
1.057MetPhe: 1.057 ± 0.267
1.219MetGly: 1.219 ± 0.282
0.244MetHis: 0.244 ± 0.114
1.463MetIle: 1.463 ± 0.415
2.276MetLys: 2.276 ± 0.373
2.52MetLeu: 2.52 ± 0.486
0.488MetMet: 0.488 ± 0.178
1.707MetAsn: 1.707 ± 0.418
0.569MetPro: 0.569 ± 0.212
0.732MetGln: 0.732 ± 0.2
0.894MetArg: 0.894 ± 0.252
1.87MetSer: 1.87 ± 0.351
1.219MetThr: 1.219 ± 0.391
0.406MetVal: 0.406 ± 0.272
0.081MetTrp: 0.081 ± 0.09
0.813MetTyr: 0.813 ± 0.299
0.0MetXaa: 0.0 ± 0.0
Asn
4.39AsnAla: 4.39 ± 0.847
0.732AsnCys: 0.732 ± 0.227
3.577AsnAsp: 3.577 ± 0.526
4.877AsnGlu: 4.877 ± 0.598
3.577AsnPhe: 3.577 ± 0.508
3.577AsnGly: 3.577 ± 0.596
0.488AsnHis: 0.488 ± 0.209
7.966AsnIle: 7.966 ± 0.981
6.747AsnLys: 6.747 ± 0.839
6.178AsnLeu: 6.178 ± 0.706
1.301AsnMet: 1.301 ± 0.352
5.121AsnAsn: 5.121 ± 0.774
1.544AsnPro: 1.544 ± 0.292
2.113AsnGln: 2.113 ± 0.357
2.601AsnArg: 2.601 ± 0.494
5.853AsnSer: 5.853 ± 0.845
4.471AsnThr: 4.471 ± 0.6
4.227AsnVal: 4.227 ± 0.532
1.544AsnTrp: 1.544 ± 0.528
2.195AsnTyr: 2.195 ± 0.465
0.0AsnXaa: 0.0 ± 0.0
Pro
1.626ProAla: 1.626 ± 0.416
0.081ProCys: 0.081 ± 0.083
0.488ProAsp: 0.488 ± 0.192
1.138ProGlu: 1.138 ± 0.343
1.138ProPhe: 1.138 ± 0.312
1.301ProGly: 1.301 ± 0.331
0.488ProHis: 0.488 ± 0.179
1.463ProIle: 1.463 ± 0.289
1.707ProLys: 1.707 ± 0.347
0.813ProLeu: 0.813 ± 0.212
0.081ProMet: 0.081 ± 0.074
1.544ProAsn: 1.544 ± 0.457
0.325ProPro: 0.325 ± 0.199
0.732ProGln: 0.732 ± 0.199
0.488ProArg: 0.488 ± 0.235
1.138ProSer: 1.138 ± 0.268
1.219ProThr: 1.219 ± 0.371
1.382ProVal: 1.382 ± 0.272
0.081ProTrp: 0.081 ± 0.08
0.732ProTyr: 0.732 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
1.707GlnAla: 1.707 ± 0.337
0.244GlnCys: 0.244 ± 0.139
1.301GlnAsp: 1.301 ± 0.28
3.089GlnGlu: 3.089 ± 0.559
1.463GlnPhe: 1.463 ± 0.427
1.463GlnGly: 1.463 ± 0.31
0.325GlnHis: 0.325 ± 0.146
3.008GlnIle: 3.008 ± 0.554
3.821GlnLys: 3.821 ± 0.609
2.276GlnLeu: 2.276 ± 0.472
0.732GlnMet: 0.732 ± 0.278
1.788GlnAsn: 1.788 ± 0.376
0.163GlnPro: 0.163 ± 0.133
0.894GlnGln: 0.894 ± 0.247
1.057GlnArg: 1.057 ± 0.265
1.463GlnSer: 1.463 ± 0.316
1.788GlnThr: 1.788 ± 0.439
1.707GlnVal: 1.707 ± 0.255
0.0GlnTrp: 0.0 ± 0.0
1.626GlnTyr: 1.626 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
2.113ArgAla: 2.113 ± 0.396
0.244ArgCys: 0.244 ± 0.143
2.52ArgAsp: 2.52 ± 0.412
3.739ArgGlu: 3.739 ± 0.73
1.138ArgPhe: 1.138 ± 0.298
2.682ArgGly: 2.682 ± 0.595
0.569ArgHis: 0.569 ± 0.202
3.008ArgIle: 3.008 ± 0.589
2.926ArgLys: 2.926 ± 0.658
2.764ArgLeu: 2.764 ± 0.654
1.057ArgMet: 1.057 ± 0.28
1.788ArgAsn: 1.788 ± 0.375
0.65ArgPro: 0.65 ± 0.324
1.463ArgGln: 1.463 ± 0.389
0.975ArgArg: 0.975 ± 0.276
0.813ArgSer: 0.813 ± 0.296
1.707ArgThr: 1.707 ± 0.469
1.951ArgVal: 1.951 ± 0.538
0.406ArgTrp: 0.406 ± 0.225
0.65ArgTyr: 0.65 ± 0.246
0.0ArgXaa: 0.0 ± 0.0
Ser
4.227SerAla: 4.227 ± 0.546
1.219SerCys: 1.219 ± 0.355
3.902SerAsp: 3.902 ± 0.679
4.877SerGlu: 4.877 ± 0.619
1.87SerPhe: 1.87 ± 0.493
3.821SerGly: 3.821 ± 0.659
0.569SerHis: 0.569 ± 0.199
5.771SerIle: 5.771 ± 0.854
5.934SerLys: 5.934 ± 0.744
5.365SerLeu: 5.365 ± 0.522
1.138SerMet: 1.138 ± 0.257
4.39SerAsn: 4.39 ± 0.852
1.626SerPro: 1.626 ± 0.385
1.87SerGln: 1.87 ± 0.399
1.463SerArg: 1.463 ± 0.433
4.308SerSer: 4.308 ± 0.81
3.902SerThr: 3.902 ± 0.701
4.227SerVal: 4.227 ± 0.572
0.813SerTrp: 0.813 ± 0.184
3.008SerTyr: 3.008 ± 0.576
0.0SerXaa: 0.0 ± 0.0
Thr
3.739ThrAla: 3.739 ± 0.652
0.325ThrCys: 0.325 ± 0.201
3.577ThrAsp: 3.577 ± 0.6
4.877ThrGlu: 4.877 ± 0.687
2.682ThrPhe: 2.682 ± 0.507
3.902ThrGly: 3.902 ± 0.46
0.488ThrHis: 0.488 ± 0.246
5.446ThrIle: 5.446 ± 0.758
5.121ThrLys: 5.121 ± 0.624
4.308ThrLeu: 4.308 ± 0.619
1.138ThrMet: 1.138 ± 0.275
2.845ThrAsn: 2.845 ± 0.466
1.301ThrPro: 1.301 ± 0.296
1.87ThrGln: 1.87 ± 0.393
1.626ThrArg: 1.626 ± 0.396
2.926ThrSer: 2.926 ± 0.538
3.821ThrThr: 3.821 ± 0.781
3.902ThrVal: 3.902 ± 0.647
0.975ThrTrp: 0.975 ± 0.26
2.357ThrTyr: 2.357 ± 0.509
0.0ThrXaa: 0.0 ± 0.0
Val
3.17ValAla: 3.17 ± 0.587
0.325ValCys: 0.325 ± 0.164
4.146ValAsp: 4.146 ± 0.691
4.959ValGlu: 4.959 ± 0.646
1.544ValPhe: 1.544 ± 0.325
3.495ValGly: 3.495 ± 0.474
0.65ValHis: 0.65 ± 0.225
3.577ValIle: 3.577 ± 0.532
5.284ValLys: 5.284 ± 0.63
4.064ValLeu: 4.064 ± 0.663
0.975ValMet: 0.975 ± 0.267
4.633ValAsn: 4.633 ± 0.624
1.951ValPro: 1.951 ± 0.369
1.382ValGln: 1.382 ± 0.316
1.626ValArg: 1.626 ± 0.458
3.902ValSer: 3.902 ± 0.642
4.715ValThr: 4.715 ± 0.845
2.601ValVal: 2.601 ± 0.433
0.325ValTrp: 0.325 ± 0.167
1.951ValTyr: 1.951 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
0.65TrpAla: 0.65 ± 0.198
0.244TrpCys: 0.244 ± 0.138
1.626TrpAsp: 1.626 ± 0.586
0.894TrpGlu: 0.894 ± 0.227
0.732TrpPhe: 0.732 ± 0.304
0.813TrpGly: 0.813 ± 0.333
0.081TrpHis: 0.081 ± 0.066
0.569TrpIle: 0.569 ± 0.251
0.813TrpLys: 0.813 ± 0.306
0.975TrpLeu: 0.975 ± 0.324
0.081TrpMet: 0.081 ± 0.08
0.65TrpAsn: 0.65 ± 0.251
0.0TrpPro: 0.0 ± 0.0
0.406TrpGln: 0.406 ± 0.246
0.163TrpArg: 0.163 ± 0.135
0.163TrpSer: 0.163 ± 0.122
0.244TrpThr: 0.244 ± 0.13
0.081TrpVal: 0.081 ± 0.078
0.163TrpTrp: 0.163 ± 0.107
0.488TrpTyr: 0.488 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.788TyrAla: 1.788 ± 0.38
0.65TyrCys: 0.65 ± 0.236
2.357TyrAsp: 2.357 ± 0.456
3.333TyrGlu: 3.333 ± 0.5
1.87TyrPhe: 1.87 ± 0.389
0.732TyrGly: 0.732 ± 0.257
0.65TyrHis: 0.65 ± 0.269
4.308TyrIle: 4.308 ± 0.779
4.796TyrLys: 4.796 ± 0.777
2.276TyrLeu: 2.276 ± 0.481
0.488TyrMet: 0.488 ± 0.221
3.17TyrAsn: 3.17 ± 0.499
0.65TyrPro: 0.65 ± 0.213
1.463TyrGln: 1.463 ± 0.295
2.113TyrArg: 2.113 ± 0.461
2.926TyrSer: 2.926 ± 0.616
3.333TyrThr: 3.333 ± 0.957
2.113TyrVal: 2.113 ± 0.414
0.325TyrTrp: 0.325 ± 0.168
1.219TyrTyr: 1.219 ± 0.427
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski