Nitrosomonas mobilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5SAJ0|A0A1G5SAJ0_9PROT Putative DNA transport competence protein ComEA OS=Nitrosomonas mobilis OX=51642 GN=NSMM_110015 PE=4 SV=1
MM1 pKa = 6.66TQTYY5 pKa = 10.54QDD7 pKa = 3.88FLNALGFRR15 pKa = 11.84EE16 pKa = 4.39SSSIPDD22 pKa = 3.47GAQNYY27 pKa = 9.53DD28 pKa = 3.33VEE30 pKa = 4.43NSFGFIGKK38 pKa = 8.38YY39 pKa = 7.97QFGEE43 pKa = 3.81AALYY47 pKa = 10.66DD48 pKa = 4.02LGYY51 pKa = 10.96YY52 pKa = 10.13GADD55 pKa = 3.28NSDD58 pKa = 3.53SNLFKK63 pKa = 10.98NDD65 pKa = 3.59WIGNWSGKK73 pKa = 9.86NGIYY77 pKa = 10.37SKK79 pKa = 10.88QDD81 pKa = 3.21YY82 pKa = 9.96LDD84 pKa = 3.62NGFVQEE90 pKa = 4.16IIIRR94 pKa = 11.84EE95 pKa = 4.17WQGVLWNRR103 pKa = 11.84IEE105 pKa = 4.67FLALDD110 pKa = 3.83KK111 pKa = 11.92YY112 pKa = 10.62EE113 pKa = 4.21GQVLNGQSITLSGMLATAHH132 pKa = 6.98LIGAGSRR139 pKa = 11.84SSDD142 pKa = 3.12TAGLKK147 pKa = 10.56GYY149 pKa = 9.47LLSGATFSPSDD160 pKa = 3.82GNGTSANDD168 pKa = 3.24YY169 pKa = 7.84MALFEE174 pKa = 4.89GFQTPFSVDD183 pKa = 3.03HH184 pKa = 6.92SIDD187 pKa = 3.72EE188 pKa = 4.74IIAGGTGNDD197 pKa = 3.04ILQGFGGNDD206 pKa = 3.26TLIGNEE212 pKa = 4.4AIDD215 pKa = 3.59VAIYY219 pKa = 8.6TGPSTNYY226 pKa = 8.44TVQRR230 pKa = 11.84QSDD233 pKa = 4.1NLWTVHH239 pKa = 5.56ARR241 pKa = 11.84NGFPDD246 pKa = 3.83GTDD249 pKa = 2.97TLIDD253 pKa = 3.25IEE255 pKa = 4.77RR256 pKa = 11.84IRR258 pKa = 11.84FSDD261 pKa = 3.49TLLALDD267 pKa = 4.64LDD269 pKa = 4.59GNAGITAKK277 pKa = 10.49IIGAVFGADD286 pKa = 3.16SVADD290 pKa = 3.84TQLAGIGLNLLDD302 pKa = 5.88DD303 pKa = 4.28GTDD306 pKa = 3.41YY307 pKa = 8.74EE308 pKa = 4.82TLMQLAIHH316 pKa = 6.71AALGDD321 pKa = 3.85EE322 pKa = 4.69AASHH326 pKa = 5.64ATVVDD331 pKa = 4.14LLYY334 pKa = 10.97EE335 pKa = 4.13NVAGFSPPAADD346 pKa = 3.41EE347 pKa = 4.42ACFVDD352 pKa = 5.22LLDD355 pKa = 4.63SGAHH359 pKa = 5.08SVASIGIFAAEE370 pKa = 3.71TAINLEE376 pKa = 4.41NINFAGLSQFGLEE389 pKa = 3.95FGLWSVV395 pKa = 3.68

Molecular weight: 42.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5SDK7|A0A1G5SDK7_9PROT Transcription elongation factor GreA OS=Nitrosomonas mobilis OX=51642 GN=greA PE=3 SV=1
MM1 pKa = 7.42ATTSTAAIIVLAVITLTRR19 pKa = 11.84MPKK22 pKa = 9.98LRR24 pKa = 11.84PNHH27 pKa = 5.54SLNRR31 pKa = 11.84IRR33 pKa = 11.84NGALLRR39 pKa = 11.84SGPFTSTLL47 pKa = 3.4

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3034

0

3034

867340

20

2969

285.9

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.311 ± 0.054

1.066 ± 0.017

5.208 ± 0.034

5.772 ± 0.042

4.068 ± 0.032

6.939 ± 0.048

2.512 ± 0.026

6.551 ± 0.038

4.494 ± 0.043

10.842 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.023

3.864 ± 0.036

4.379 ± 0.028

4.467 ± 0.034

5.866 ± 0.035

6.064 ± 0.036

5.419 ± 0.035

6.558 ± 0.043

1.304 ± 0.021

2.79 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski