Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium cellulovorans

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9SPW3|D9SPW3_CLOC7 Uncharacterized protein OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=Clocel_2383 PE=4 SV=1
MM1 pKa = 7.56ILTYY5 pKa = 9.22SEE7 pKa = 4.95KK8 pKa = 10.96GDD10 pKa = 3.67GQDD13 pKa = 4.48IIVDD17 pKa = 3.54YY18 pKa = 11.08SGEE21 pKa = 4.19ANTIIDD27 pKa = 3.55TYY29 pKa = 10.64IFRR32 pKa = 11.84KK33 pKa = 10.4GYY35 pKa = 8.9GQVNIYY41 pKa = 9.91EE42 pKa = 4.27YY43 pKa = 10.87SGEE46 pKa = 4.24ANTIIDD52 pKa = 3.98TLKK55 pKa = 11.24LEE57 pKa = 5.3DD58 pKa = 4.12INPNEE63 pKa = 4.44ISLNCNRR70 pKa = 11.84YY71 pKa = 9.81DD72 pKa = 5.82LIMKK76 pKa = 10.19LNEE79 pKa = 4.33SKK81 pKa = 11.06DD82 pKa = 3.71SITIQGYY89 pKa = 9.42FSSDD93 pKa = 2.57LHH95 pKa = 8.25KK96 pKa = 10.45IEE98 pKa = 5.57KK99 pKa = 9.92IQFADD104 pKa = 3.76GTVWDD109 pKa = 4.14INTIKK114 pKa = 10.8SSLVYY119 pKa = 10.98SNGTVGIDD127 pKa = 3.27SLNAEE132 pKa = 4.94GEE134 pKa = 4.51VIMNGYY140 pKa = 9.89EE141 pKa = 4.05GDD143 pKa = 3.55DD144 pKa = 3.78TLRR147 pKa = 11.84SGAGNDD153 pKa = 3.68KK154 pKa = 10.69IYY156 pKa = 11.07GGTGNDD162 pKa = 2.88QVYY165 pKa = 10.95GNGGNDD171 pKa = 3.63LLYY174 pKa = 11.3GEE176 pKa = 5.81DD177 pKa = 5.05GDD179 pKa = 4.75DD180 pKa = 4.39KK181 pKa = 11.54LYY183 pKa = 11.24GGNGDD188 pKa = 5.14DD189 pKa = 4.78ILDD192 pKa = 3.97GGEE195 pKa = 4.22GKK197 pKa = 10.29DD198 pKa = 3.54YY199 pKa = 11.16LYY201 pKa = 11.39GEE203 pKa = 4.71NGNDD207 pKa = 4.56LLDD210 pKa = 4.12GKK212 pKa = 10.6NGGGYY217 pKa = 10.26LEE219 pKa = 5.26GGAGVDD225 pKa = 3.21TYY227 pKa = 11.08IFRR230 pKa = 11.84KK231 pKa = 10.39GYY233 pKa = 8.9GQVNIYY239 pKa = 9.91EE240 pKa = 4.27YY241 pKa = 10.87SGEE244 pKa = 4.24ANTIIDD250 pKa = 3.98TLKK253 pKa = 11.24LEE255 pKa = 5.3DD256 pKa = 4.12INPNEE261 pKa = 4.44ISLNCNRR268 pKa = 11.84YY269 pKa = 9.81DD270 pKa = 5.82LIMKK274 pKa = 10.19LNEE277 pKa = 4.33SKK279 pKa = 11.06DD280 pKa = 3.71SITIQGYY287 pKa = 9.42FSSDD291 pKa = 2.57LHH293 pKa = 8.25KK294 pKa = 10.45IEE296 pKa = 5.57KK297 pKa = 9.92IQFADD302 pKa = 3.76GTVWDD307 pKa = 4.14INTIKK312 pKa = 10.8SSLVYY317 pKa = 10.98SNGTVGIDD325 pKa = 3.27SLNAEE330 pKa = 4.94GEE332 pKa = 4.51VIMNGYY338 pKa = 9.89EE339 pKa = 4.05GDD341 pKa = 3.55DD342 pKa = 3.78TLRR345 pKa = 11.84SGAGNDD351 pKa = 3.68KK352 pKa = 10.69IYY354 pKa = 11.07GGTGNDD360 pKa = 2.88QVYY363 pKa = 10.95GNGGNDD369 pKa = 3.63LLYY372 pKa = 11.3GEE374 pKa = 5.81DD375 pKa = 5.05GDD377 pKa = 4.75DD378 pKa = 4.39KK379 pKa = 11.54LYY381 pKa = 11.24GGNGDD386 pKa = 5.14DD387 pKa = 4.78ILDD390 pKa = 3.97GGEE393 pKa = 4.22GKK395 pKa = 10.29DD396 pKa = 3.54YY397 pKa = 11.16LYY399 pKa = 11.39GEE401 pKa = 4.71NGNDD405 pKa = 4.56LLDD408 pKa = 4.12GKK410 pKa = 10.6NGGGYY415 pKa = 10.45LEE417 pKa = 5.18GGAGNDD423 pKa = 3.28TYY425 pKa = 11.04IFSKK429 pKa = 11.08GDD431 pKa = 3.49GQDD434 pKa = 2.95IIYY437 pKa = 10.53NSSSLDD443 pKa = 3.29TDD445 pKa = 3.39NDD447 pKa = 3.87KK448 pKa = 11.58LSLNDD453 pKa = 3.35VNLSEE458 pKa = 4.21TAFIKK463 pKa = 11.02SNTDD467 pKa = 4.01LIIKK471 pKa = 10.2LNEE474 pKa = 4.0TTDD477 pKa = 3.26SVTILGYY484 pKa = 10.45FNYY487 pKa = 10.25GKK489 pKa = 10.8YY490 pKa = 10.19SLEE493 pKa = 4.2EE494 pKa = 3.74IEE496 pKa = 4.76FMNGEE501 pKa = 4.36TLTPEE506 pKa = 4.2DD507 pKa = 4.04VNNVILGKK515 pKa = 10.13YY516 pKa = 8.84VSHH519 pKa = 7.65TIPQYY524 pKa = 11.02NISLDD529 pKa = 3.46KK530 pKa = 10.98AIEE533 pKa = 4.08ILSEE537 pKa = 3.98ASNPSIFNITGTSTSSDD554 pKa = 2.6IMTDD558 pKa = 2.4ILLNMNSS565 pKa = 3.3

Molecular weight: 61.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9SRQ7|D9SRQ7_CLOC7 Uncharacterized protein OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=Clocel_0653 PE=4 SV=1
MM1 pKa = 7.5LKK3 pKa = 10.2SKK5 pKa = 10.87KK6 pKa = 10.42NIIARR11 pKa = 11.84LRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 9.15IVNLPISKK23 pKa = 9.71FPYY26 pKa = 8.08YY27 pKa = 10.21RR28 pKa = 11.84LYY30 pKa = 10.75RR31 pKa = 11.84PTYY34 pKa = 10.09ALTLPEE40 pKa = 4.0VNRR43 pKa = 11.84LKK45 pKa = 10.72GRR47 pKa = 11.84RR48 pKa = 11.84IRR50 pKa = 11.84TTVPNIVGTITATVGEE66 pKa = 4.71YY67 pKa = 10.66NPNTNRR73 pKa = 11.84VEE75 pKa = 4.19LKK77 pKa = 10.8NIISDD82 pKa = 3.74RR83 pKa = 11.84TGVSYY88 pKa = 11.66GNLSYY93 pKa = 11.26LLDD96 pKa = 5.04EE97 pKa = 4.57IAGLQVLPKK106 pKa = 10.97DD107 pKa = 3.34NGTPGGSKK115 pKa = 10.2DD116 pKa = 3.68CQITGGKK123 pKa = 9.06GKK125 pKa = 10.59YY126 pKa = 7.59ITSGEE131 pKa = 4.12TSIGNVSVKK140 pKa = 8.62YY141 pKa = 8.95TIHH144 pKa = 6.25EE145 pKa = 4.2CMIEE149 pKa = 4.05IIPRR153 pKa = 11.84VDD155 pKa = 3.17GLPPRR160 pKa = 11.84RR161 pKa = 11.84LIINRR166 pKa = 11.84NNTRR170 pKa = 11.84VQTMFRR176 pKa = 11.84TSPRR180 pKa = 11.84NRR182 pKa = 11.84IKK184 pKa = 9.85FTAWIQNNTVWVEE197 pKa = 3.25IEE199 pKa = 3.93YY200 pKa = 10.31QRR202 pKa = 11.84RR203 pKa = 11.84TRR205 pKa = 11.84FGRR208 pKa = 11.84WQRR211 pKa = 11.84INEE214 pKa = 3.86VRR216 pKa = 11.84TKK218 pKa = 10.89LGTWQDD224 pKa = 3.49LKK226 pKa = 11.09II227 pKa = 4.32

Molecular weight: 26.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4211

0

4211

1429936

30

9858

339.6

38.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.863 ± 0.041

1.107 ± 0.014

5.72 ± 0.034

7.362 ± 0.06

4.348 ± 0.031

6.356 ± 0.049

1.374 ± 0.016

9.486 ± 0.048

8.465 ± 0.041

8.789 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.018

6.273 ± 0.036

2.782 ± 0.024

2.585 ± 0.019

3.304 ± 0.025

6.571 ± 0.037

5.536 ± 0.046

6.531 ± 0.033

0.817 ± 0.014

4.305 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski