Amino acid dipepetide frequency for Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.005AlaAla: 4.005 ± 0.074
0.642AlaCys: 0.642 ± 0.022
2.934AlaAsp: 2.934 ± 0.054
3.859AlaGlu: 3.859 ± 0.075
2.651AlaPhe: 2.651 ± 0.052
3.704AlaGly: 3.704 ± 0.071
0.792AlaHis: 0.792 ± 0.026
5.883AlaIle: 5.883 ± 0.085
4.63AlaLys: 4.63 ± 0.061
5.8AlaLeu: 5.8 ± 0.079
1.676AlaMet: 1.676 ± 0.039
2.892AlaAsn: 2.892 ± 0.053
1.415AlaPro: 1.415 ± 0.034
1.536AlaGln: 1.536 ± 0.032
1.943AlaArg: 1.943 ± 0.038
3.545AlaSer: 3.545 ± 0.056
3.623AlaThr: 3.623 ± 0.082
4.324AlaVal: 4.324 ± 0.067
0.45AlaTrp: 0.45 ± 0.018
2.325AlaTyr: 2.325 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.611CysAla: 0.611 ± 0.022
0.194CysCys: 0.194 ± 0.019
0.703CysAsp: 0.703 ± 0.024
0.74CysGlu: 0.74 ± 0.027
0.48CysPhe: 0.48 ± 0.02
0.994CysGly: 0.994 ± 0.031
0.23CysHis: 0.23 ± 0.011
0.955CysIle: 0.955 ± 0.028
0.862CysLys: 0.862 ± 0.029
0.773CysLeu: 0.773 ± 0.022
0.22CysMet: 0.22 ± 0.013
0.676CysAsn: 0.676 ± 0.024
0.416CysPro: 0.416 ± 0.019
0.244CysGln: 0.244 ± 0.014
0.37CysArg: 0.37 ± 0.019
0.795CysSer: 0.795 ± 0.025
0.616CysThr: 0.616 ± 0.025
0.609CysVal: 0.609 ± 0.024
0.095CysTrp: 0.095 ± 0.008
0.483CysTyr: 0.483 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
2.921AspAla: 2.921 ± 0.054
0.573AspCys: 0.573 ± 0.02
2.92AspAsp: 2.92 ± 0.065
4.417AspGlu: 4.417 ± 0.064
2.825AspPhe: 2.825 ± 0.046
3.822AspGly: 3.822 ± 0.083
0.578AspHis: 0.578 ± 0.02
6.165AspIle: 6.165 ± 0.082
5.208AspLys: 5.208 ± 0.071
4.775AspLeu: 4.775 ± 0.06
1.435AspMet: 1.435 ± 0.037
3.607AspAsn: 3.607 ± 0.054
1.418AspPro: 1.418 ± 0.039
1.046AspGln: 1.046 ± 0.034
1.804AspArg: 1.804 ± 0.041
3.564AspSer: 3.564 ± 0.066
3.176AspThr: 3.176 ± 0.075
3.633AspVal: 3.633 ± 0.063
0.443AspTrp: 0.443 ± 0.017
2.871AspTyr: 2.871 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
4.574GluAla: 4.574 ± 0.075
0.587GluCys: 0.587 ± 0.024
4.234GluAsp: 4.234 ± 0.069
6.874GluGlu: 6.874 ± 0.123
3.037GluPhe: 3.037 ± 0.052
4.026GluGly: 4.026 ± 0.062
0.932GluHis: 0.932 ± 0.026
6.995GluIle: 6.995 ± 0.095
7.182GluLys: 7.182 ± 0.084
6.702GluLeu: 6.702 ± 0.08
1.848GluMet: 1.848 ± 0.041
5.121GluAsn: 5.121 ± 0.065
1.479GluPro: 1.479 ± 0.033
1.932GluGln: 1.932 ± 0.044
2.563GluArg: 2.563 ± 0.041
3.536GluSer: 3.536 ± 0.055
3.523GluThr: 3.523 ± 0.053
4.96GluVal: 4.96 ± 0.067
0.473GluTrp: 0.473 ± 0.021
3.048GluTyr: 3.048 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
2.322PheAla: 2.322 ± 0.047
0.52PheCys: 0.52 ± 0.022
2.762PheAsp: 2.762 ± 0.047
2.784PheGlu: 2.784 ± 0.057
1.999PhePhe: 1.999 ± 0.042
2.732PheGly: 2.732 ± 0.051
0.601PheHis: 0.601 ± 0.023
4.313PheIle: 4.313 ± 0.067
3.574PheLys: 3.574 ± 0.052
3.678PheLeu: 3.678 ± 0.06
1.088PheMet: 1.088 ± 0.025
2.871PheAsn: 2.871 ± 0.049
1.186PhePro: 1.186 ± 0.034
1.2PheGln: 1.2 ± 0.026
1.293PheArg: 1.293 ± 0.032
3.274PheSer: 3.274 ± 0.053
2.371PheThr: 2.371 ± 0.039
2.644PheVal: 2.644 ± 0.049
0.359PheTrp: 0.359 ± 0.02
1.906PheTyr: 1.906 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
3.762GlyAla: 3.762 ± 0.07
0.833GlyCys: 0.833 ± 0.026
3.591GlyAsp: 3.591 ± 0.062
4.329GlyGlu: 4.329 ± 0.071
3.059GlyPhe: 3.059 ± 0.048
4.099GlyGly: 4.099 ± 0.089
0.996GlyHis: 0.996 ± 0.029
6.302GlyIle: 6.302 ± 0.073
5.261GlyLys: 5.261 ± 0.065
4.875GlyLeu: 4.875 ± 0.061
1.564GlyMet: 1.564 ± 0.035
3.763GlyAsn: 3.763 ± 0.1
1.136GlyPro: 1.136 ± 0.031
1.624GlyGln: 1.624 ± 0.039
2.146GlyArg: 2.146 ± 0.042
3.831GlySer: 3.831 ± 0.063
4.102GlyThr: 4.102 ± 0.087
4.482GlyVal: 4.482 ± 0.069
0.611GlyTrp: 0.611 ± 0.025
3.198GlyTyr: 3.198 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
0.634HisAla: 0.634 ± 0.02
0.232HisCys: 0.232 ± 0.013
0.695HisAsp: 0.695 ± 0.027
0.843HisGlu: 0.843 ± 0.027
0.646HisPhe: 0.646 ± 0.023
1.038HisGly: 1.038 ± 0.031
0.342HisHis: 0.342 ± 0.02
1.305HisIle: 1.305 ± 0.029
1.048HisLys: 1.048 ± 0.028
1.194HisLeu: 1.194 ± 0.028
0.345HisMet: 0.345 ± 0.017
0.796HisAsn: 0.796 ± 0.022
0.594HisPro: 0.594 ± 0.023
0.391HisGln: 0.391 ± 0.018
0.552HisArg: 0.552 ± 0.02
0.913HisSer: 0.913 ± 0.023
0.667HisThr: 0.667 ± 0.023
0.737HisVal: 0.737 ± 0.025
0.145HisTrp: 0.145 ± 0.011
0.62HisTyr: 0.62 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.834IleAla: 5.834 ± 0.072
1.101IleCys: 1.101 ± 0.029
6.137IleAsp: 6.137 ± 0.071
7.075IleGlu: 7.075 ± 0.086
4.076IlePhe: 4.076 ± 0.068
5.637IleGly: 5.637 ± 0.075
1.262IleHis: 1.262 ± 0.032
8.547IleIle: 8.547 ± 0.108
8.016IleLys: 8.016 ± 0.08
8.177IleLeu: 8.177 ± 0.106
2.069IleMet: 2.069 ± 0.044
6.236IleAsn: 6.236 ± 0.081
3.113IlePro: 3.113 ± 0.055
2.314IleGln: 2.314 ± 0.041
2.912IleArg: 2.912 ± 0.049
6.977IleSer: 6.977 ± 0.08
5.338IleThr: 5.338 ± 0.066
5.809IleVal: 5.809 ± 0.083
0.631IleTrp: 0.631 ± 0.022
3.603IleTyr: 3.603 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
5.276LysAla: 5.276 ± 0.074
0.737LysCys: 0.737 ± 0.026
5.43LysAsp: 5.43 ± 0.07
8.095LysGlu: 8.095 ± 0.105
2.917LysPhe: 2.917 ± 0.046
4.898LysGly: 4.898 ± 0.068
1.118LysHis: 1.118 ± 0.031
7.646LysIle: 7.646 ± 0.075
7.046LysLys: 7.046 ± 0.092
7.292LysLeu: 7.292 ± 0.072
1.96LysMet: 1.96 ± 0.042
5.726LysAsn: 5.726 ± 0.069
2.069LysPro: 2.069 ± 0.04
2.164LysGln: 2.164 ± 0.04
2.741LysArg: 2.741 ± 0.045
4.807LysSer: 4.807 ± 0.054
4.251LysThr: 4.251 ± 0.063
5.973LysVal: 5.973 ± 0.072
0.648LysTrp: 0.648 ± 0.023
3.858LysTyr: 3.858 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
5.307LeuAla: 5.307 ± 0.071
1.062LeuCys: 1.062 ± 0.031
4.985LeuAsp: 4.985 ± 0.061
6.249LeuGlu: 6.249 ± 0.076
3.544LeuPhe: 3.544 ± 0.059
5.652LeuGly: 5.652 ± 0.072
1.108LeuHis: 1.108 ± 0.027
7.66LeuIle: 7.66 ± 0.089
8.091LeuLys: 8.091 ± 0.095
7.795LeuLeu: 7.795 ± 0.098
2.187LeuMet: 2.187 ± 0.041
5.39LeuAsn: 5.39 ± 0.068
2.782LeuPro: 2.782 ± 0.052
2.42LeuGln: 2.42 ± 0.043
3.099LeuArg: 3.099 ± 0.051
6.677LeuSer: 6.677 ± 0.077
4.69LeuThr: 4.69 ± 0.068
5.3LeuVal: 5.3 ± 0.065
0.636LeuTrp: 0.636 ± 0.022
3.255LeuTyr: 3.255 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
1.564MetAla: 1.564 ± 0.037
0.238MetCys: 0.238 ± 0.013
1.481MetAsp: 1.481 ± 0.033
1.692MetGlu: 1.692 ± 0.036
0.948MetPhe: 0.948 ± 0.029
1.487MetGly: 1.487 ± 0.038
0.337MetHis: 0.337 ± 0.016
2.089MetIle: 2.089 ± 0.042
2.391MetLys: 2.391 ± 0.042
2.125MetLeu: 2.125 ± 0.041
0.626MetMet: 0.626 ± 0.021
1.509MetAsn: 1.509 ± 0.036
0.869MetPro: 0.869 ± 0.028
0.704MetGln: 0.704 ± 0.021
0.872MetArg: 0.872 ± 0.027
1.561MetSer: 1.561 ± 0.033
1.158MetThr: 1.158 ± 0.03
1.546MetVal: 1.546 ± 0.033
0.165MetTrp: 0.165 ± 0.011
0.873MetTyr: 0.873 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.211AsnAla: 3.211 ± 0.06
0.743AsnCys: 0.743 ± 0.028
3.336AsnAsp: 3.336 ± 0.091
4.148AsnGlu: 4.148 ± 0.06
2.644AsnPhe: 2.644 ± 0.049
4.035AsnGly: 4.035 ± 0.076
0.915AsnHis: 0.915 ± 0.025
6.435AsnIle: 6.435 ± 0.069
5.096AsnLys: 5.096 ± 0.067
5.711AsnLeu: 5.711 ± 0.073
1.406AsnMet: 1.406 ± 0.03
4.323AsnAsn: 4.323 ± 0.067
2.22AsnPro: 2.22 ± 0.048
1.667AsnGln: 1.667 ± 0.036
2.033AsnArg: 2.033 ± 0.04
4.398AsnSer: 4.398 ± 0.062
3.381AsnThr: 3.381 ± 0.065
3.636AsnVal: 3.636 ± 0.06
0.515AsnTrp: 0.515 ± 0.019
2.878AsnTyr: 2.878 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
1.46ProAla: 1.46 ± 0.041
0.29ProCys: 0.29 ± 0.016
1.432ProAsp: 1.432 ± 0.036
2.188ProGlu: 2.188 ± 0.044
1.28ProPhe: 1.28 ± 0.035
1.592ProGly: 1.592 ± 0.043
0.438ProHis: 0.438 ± 0.018
2.583ProIle: 2.583 ± 0.043
2.151ProLys: 2.151 ± 0.045
2.409ProLeu: 2.409 ± 0.042
0.717ProMet: 0.717 ± 0.023
1.608ProAsn: 1.608 ± 0.036
0.608ProPro: 0.608 ± 0.021
0.842ProGln: 0.842 ± 0.024
0.852ProArg: 0.852 ± 0.024
1.905ProSer: 1.905 ± 0.044
1.815ProThr: 1.815 ± 0.048
2.087ProVal: 2.087 ± 0.049
0.283ProTrp: 0.283 ± 0.016
1.283ProTyr: 1.283 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
1.413GlnAla: 1.413 ± 0.033
0.32GlnCys: 0.32 ± 0.018
1.311GlnAsp: 1.311 ± 0.034
2.002GlnGlu: 2.002 ± 0.039
1.056GlnPhe: 1.056 ± 0.026
1.866GlnGly: 1.866 ± 0.037
0.327GlnHis: 0.327 ± 0.014
2.127GlnIle: 2.127 ± 0.046
2.083GlnLys: 2.083 ± 0.039
2.501GlnLeu: 2.501 ± 0.044
0.671GlnMet: 0.671 ± 0.025
1.607GlnAsn: 1.607 ± 0.035
0.588GlnPro: 0.588 ± 0.021
0.816GlnGln: 0.816 ± 0.026
1.009GlnArg: 1.009 ± 0.029
1.511GlnSer: 1.511 ± 0.036
1.197GlnThr: 1.197 ± 0.035
1.787GlnVal: 1.787 ± 0.035
0.358GlnTrp: 0.358 ± 0.017
1.297GlnTyr: 1.297 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
1.79ArgAla: 1.79 ± 0.038
0.384ArgCys: 0.384 ± 0.018
1.806ArgAsp: 1.806 ± 0.038
2.69ArgGlu: 2.69 ± 0.051
1.466ArgPhe: 1.466 ± 0.036
1.92ArgGly: 1.92 ± 0.035
0.486ArgHis: 0.486 ± 0.017
3.264ArgIle: 3.264 ± 0.052
2.927ArgLys: 2.927 ± 0.052
2.936ArgLeu: 2.936 ± 0.058
0.899ArgMet: 0.899 ± 0.026
2.157ArgAsn: 2.157 ± 0.041
0.834ArgPro: 0.834 ± 0.028
0.886ArgGln: 0.886 ± 0.024
1.346ArgArg: 1.346 ± 0.035
1.655ArgSer: 1.655 ± 0.036
1.678ArgThr: 1.678 ± 0.035
2.132ArgVal: 2.132 ± 0.041
0.316ArgTrp: 0.316 ± 0.016
1.47ArgTyr: 1.47 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.56SerAla: 3.56 ± 0.064
0.72SerCys: 0.72 ± 0.026
3.447SerAsp: 3.447 ± 0.051
4.288SerGlu: 4.288 ± 0.061
3.101SerPhe: 3.101 ± 0.045
4.515SerGly: 4.515 ± 0.068
0.873SerHis: 0.873 ± 0.024
6.464SerIle: 6.464 ± 0.075
5.661SerLys: 5.661 ± 0.07
5.84SerLeu: 5.84 ± 0.077
1.595SerMet: 1.595 ± 0.036
4.023SerAsn: 4.023 ± 0.058
1.683SerPro: 1.683 ± 0.038
1.73SerGln: 1.73 ± 0.034
2.119SerArg: 2.119 ± 0.038
4.765SerSer: 4.765 ± 0.081
3.737SerThr: 3.737 ± 0.074
4.073SerVal: 4.073 ± 0.064
0.578SerTrp: 0.578 ± 0.021
2.943SerTyr: 2.943 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
3.596ThrAla: 3.596 ± 0.07
0.513ThrCys: 0.513 ± 0.022
2.836ThrAsp: 2.836 ± 0.062
3.476ThrGlu: 3.476 ± 0.053
2.437ThrPhe: 2.437 ± 0.044
3.887ThrGly: 3.887 ± 0.07
0.759ThrHis: 0.759 ± 0.022
5.432ThrIle: 5.432 ± 0.079
3.948ThrLys: 3.948 ± 0.057
5.245ThrLeu: 5.245 ± 0.079
1.229ThrMet: 1.229 ± 0.027
3.161ThrAsn: 3.161 ± 0.068
1.932ThrPro: 1.932 ± 0.046
1.222ThrGln: 1.222 ± 0.032
1.569ThrArg: 1.569 ± 0.035
3.825ThrSer: 3.825 ± 0.069
3.467ThrThr: 3.467 ± 0.098
3.996ThrVal: 3.996 ± 0.082
0.512ThrTrp: 0.512 ± 0.023
2.313ThrTyr: 2.313 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
4.289ValAla: 4.289 ± 0.063
0.695ValCys: 0.695 ± 0.022
3.994ValAsp: 3.994 ± 0.059
4.466ValGlu: 4.466 ± 0.066
2.9ValPhe: 2.9 ± 0.042
4.106ValGly: 4.106 ± 0.059
0.845ValHis: 0.845 ± 0.026
5.973ValIle: 5.973 ± 0.078
5.186ValLys: 5.186 ± 0.075
5.756ValLeu: 5.756 ± 0.073
1.513ValMet: 1.513 ± 0.035
3.841ValAsn: 3.841 ± 0.061
1.986ValPro: 1.986 ± 0.043
1.697ValGln: 1.697 ± 0.039
2.015ValArg: 2.015 ± 0.036
4.52ValSer: 4.52 ± 0.067
3.936ValThr: 3.936 ± 0.084
4.659ValVal: 4.659 ± 0.081
0.464ValTrp: 0.464 ± 0.022
2.469ValTyr: 2.469 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.461TrpAla: 0.461 ± 0.021
0.126TrpCys: 0.126 ± 0.009
0.519TrpAsp: 0.519 ± 0.022
0.491TrpGlu: 0.491 ± 0.021
0.352TrpPhe: 0.352 ± 0.017
0.631TrpGly: 0.631 ± 0.023
0.166TrpHis: 0.166 ± 0.011
0.691TrpIle: 0.691 ± 0.024
0.546TrpLys: 0.546 ± 0.022
0.657TrpLeu: 0.657 ± 0.024
0.216TrpMet: 0.216 ± 0.012
0.58TrpAsn: 0.58 ± 0.022
0.16TrpPro: 0.16 ± 0.011
0.315TrpGln: 0.315 ± 0.015
0.293TrpArg: 0.293 ± 0.014
0.587TrpSer: 0.587 ± 0.023
0.429TrpThr: 0.429 ± 0.018
0.49TrpVal: 0.49 ± 0.021
0.107TrpTrp: 0.107 ± 0.008
0.353TrpTyr: 0.353 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.041TyrAla: 2.041 ± 0.043
0.556TyrCys: 0.556 ± 0.02
2.65TyrAsp: 2.65 ± 0.072
2.918TyrGlu: 2.918 ± 0.051
2.062TyrPhe: 2.062 ± 0.041
2.921TyrGly: 2.921 ± 0.067
0.634TyrHis: 0.634 ± 0.02
4.034TyrIle: 4.034 ± 0.055
3.744TyrLys: 3.744 ± 0.057
3.648TyrLeu: 3.648 ± 0.058
0.97TyrMet: 0.97 ± 0.029
2.843TyrAsn: 2.843 ± 0.054
1.334TyrPro: 1.334 ± 0.034
1.099TyrGln: 1.099 ± 0.033
1.511TyrArg: 1.511 ± 0.032
3.065TyrSer: 3.065 ± 0.059
2.201TyrThr: 2.201 ± 0.042
2.435TyrVal: 2.435 ± 0.048
0.381TyrTrp: 0.381 ± 0.017
2.005TyrTyr: 2.005 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4211 proteins (1429936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski