Gordonia phage Neville

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DYK6|A0A385DYK6_9CAUD Membrane protein OS=Gordonia phage Neville OX=2301693 GN=131 PE=4 SV=1
MM1 pKa = 7.4TSIQALHH8 pKa = 5.57EE9 pKa = 4.23QEE11 pKa = 3.93VDD13 pKa = 2.78EE14 pKa = 4.91AYY16 pKa = 10.52RR17 pKa = 11.84RR18 pKa = 11.84GYY20 pKa = 10.7DD21 pKa = 2.95EE22 pKa = 6.24GYY24 pKa = 10.91DD25 pKa = 3.4EE26 pKa = 5.27GRR28 pKa = 11.84EE29 pKa = 3.87DD30 pKa = 4.6GYY32 pKa = 11.69NDD34 pKa = 3.98GYY36 pKa = 11.17EE37 pKa = 4.55DD38 pKa = 4.53GSAEE42 pKa = 4.28PIAA45 pKa = 5.22

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DYD9|A0A385DYD9_9CAUD Uncharacterized protein OS=Gordonia phage Neville OX=2301693 GN=109 PE=4 SV=1
MM1 pKa = 7.13TVKK4 pKa = 10.41QAVRR8 pKa = 11.84YY9 pKa = 6.29MTSEE13 pKa = 4.12DD14 pKa = 3.51VAEE17 pKa = 4.46SLGTTPKK24 pKa = 10.34NVRR27 pKa = 11.84NKK29 pKa = 9.95AVEE32 pKa = 3.99YY33 pKa = 10.42VQTQGRR39 pKa = 11.84RR40 pKa = 11.84GLRR43 pKa = 11.84GFQTGPKK50 pKa = 9.07GRR52 pKa = 11.84WRR54 pKa = 11.84FRR56 pKa = 11.84PSDD59 pKa = 3.47VEE61 pKa = 4.16AFAEE65 pKa = 4.52GRR67 pKa = 11.84LVKK70 pKa = 10.72

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

23539

25

2278

182.5

20.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.992 ± 0.365

0.922 ± 0.13

6.789 ± 0.197

6.054 ± 0.229

3.535 ± 0.113

8.611 ± 0.4

2.099 ± 0.173

4.495 ± 0.149

4.32 ± 0.231

7.957 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.09

3.352 ± 0.219

5.276 ± 0.224

3.501 ± 0.172

6.202 ± 0.182

5.952 ± 0.189

6.033 ± 0.204

7.383 ± 0.227

2.162 ± 0.117

2.863 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski