Amino acid dipepetide frequency for Gordonia phage Neville

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.409AlaAla: 10.409 ± 0.94
0.765AlaCys: 0.765 ± 0.202
5.99AlaAsp: 5.99 ± 0.565
6.713AlaGlu: 6.713 ± 0.672
3.101AlaPhe: 3.101 ± 0.336
8.2AlaGly: 8.2 ± 0.604
1.827AlaHis: 1.827 ± 0.252
4.121AlaIle: 4.121 ± 0.446
4.588AlaLys: 4.588 ± 0.427
8.794AlaLeu: 8.794 ± 0.694
3.399AlaMet: 3.399 ± 0.421
3.059AlaAsn: 3.059 ± 0.413
4.503AlaPro: 4.503 ± 0.501
3.696AlaGln: 3.696 ± 0.361
6.458AlaArg: 6.458 ± 0.551
4.928AlaSer: 4.928 ± 0.429
6.967AlaThr: 6.967 ± 0.666
7.392AlaVal: 7.392 ± 0.513
2.507AlaTrp: 2.507 ± 0.275
2.507AlaTyr: 2.507 ± 0.347
0.0AlaXaa: 0.0 ± 0.0
Cys
0.85CysAla: 0.85 ± 0.183
0.17CysCys: 0.17 ± 0.132
0.595CysAsp: 0.595 ± 0.17
0.51CysGlu: 0.51 ± 0.166
0.127CysPhe: 0.127 ± 0.075
1.147CysGly: 1.147 ± 0.225
0.127CysHis: 0.127 ± 0.071
0.467CysIle: 0.467 ± 0.165
0.467CysLys: 0.467 ± 0.158
0.765CysLeu: 0.765 ± 0.226
0.255CysMet: 0.255 ± 0.107
0.297CysAsn: 0.297 ± 0.134
0.51CysPro: 0.51 ± 0.183
0.34CysGln: 0.34 ± 0.109
0.722CysArg: 0.722 ± 0.204
0.425CysSer: 0.425 ± 0.121
0.425CysThr: 0.425 ± 0.133
0.595CysVal: 0.595 ± 0.166
0.127CysTrp: 0.127 ± 0.078
0.297CysTyr: 0.297 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
7.137AspAla: 7.137 ± 0.554
0.68AspCys: 0.68 ± 0.156
6.075AspAsp: 6.075 ± 0.615
4.971AspGlu: 4.971 ± 0.668
1.869AspPhe: 1.869 ± 0.273
6.415AspGly: 6.415 ± 0.52
1.36AspHis: 1.36 ± 0.187
2.846AspIle: 2.846 ± 0.321
2.337AspLys: 2.337 ± 0.387
5.141AspLeu: 5.141 ± 0.425
1.742AspMet: 1.742 ± 0.311
1.912AspAsn: 1.912 ± 0.355
4.376AspPro: 4.376 ± 0.441
1.954AspGln: 1.954 ± 0.342
3.611AspArg: 3.611 ± 0.396
4.163AspSer: 4.163 ± 0.543
3.016AspThr: 3.016 ± 0.346
4.631AspVal: 4.631 ± 0.426
1.699AspTrp: 1.699 ± 0.26
1.954AspTyr: 1.954 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
5.183GluAla: 5.183 ± 0.5
0.722GluCys: 0.722 ± 0.184
3.229GluAsp: 3.229 ± 0.387
3.016GluGlu: 3.016 ± 0.389
3.101GluPhe: 3.101 ± 0.32
3.526GluGly: 3.526 ± 0.451
1.784GluHis: 1.784 ± 0.36
3.016GluIle: 3.016 ± 0.372
2.167GluLys: 2.167 ± 0.294
6.245GluLeu: 6.245 ± 0.689
1.444GluMet: 1.444 ± 0.241
2.294GluAsn: 2.294 ± 0.291
3.399GluPro: 3.399 ± 0.628
2.209GluGln: 2.209 ± 0.313
4.546GluArg: 4.546 ± 0.416
3.484GluSer: 3.484 ± 0.336
3.314GluThr: 3.314 ± 0.399
4.248GluVal: 4.248 ± 0.431
1.657GluTrp: 1.657 ± 0.238
1.954GluTyr: 1.954 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
3.101PheAla: 3.101 ± 0.395
0.297PheCys: 0.297 ± 0.111
2.209PheAsp: 2.209 ± 0.236
2.337PheGlu: 2.337 ± 0.291
0.977PhePhe: 0.977 ± 0.167
3.824PheGly: 3.824 ± 0.372
0.68PheHis: 0.68 ± 0.213
1.657PheIle: 1.657 ± 0.287
1.275PheLys: 1.275 ± 0.22
2.252PheLeu: 2.252 ± 0.31
1.105PheMet: 1.105 ± 0.241
1.402PheAsn: 1.402 ± 0.256
1.699PhePro: 1.699 ± 0.25
1.402PheGln: 1.402 ± 0.202
1.954PheArg: 1.954 ± 0.287
2.507PheSer: 2.507 ± 0.303
2.464PheThr: 2.464 ± 0.34
2.719PheVal: 2.719 ± 0.445
0.595PheTrp: 0.595 ± 0.148
0.892PheTyr: 0.892 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
6.628GlyAla: 6.628 ± 0.681
0.595GlyCys: 0.595 ± 0.212
5.693GlyAsp: 5.693 ± 0.679
5.311GlyGlu: 5.311 ± 0.545
3.441GlyPhe: 3.441 ± 0.436
8.115GlyGly: 8.115 ± 1.034
1.614GlyHis: 1.614 ± 0.294
4.631GlyIle: 4.631 ± 0.516
4.503GlyLys: 4.503 ± 0.462
5.565GlyLeu: 5.565 ± 0.399
2.592GlyMet: 2.592 ± 0.33
2.379GlyAsn: 2.379 ± 0.387
3.739GlyPro: 3.739 ± 0.435
3.314GlyGln: 3.314 ± 0.48
4.843GlyArg: 4.843 ± 0.439
5.863GlySer: 5.863 ± 0.549
5.438GlyThr: 5.438 ± 0.458
6.585GlyVal: 6.585 ± 0.474
1.869GlyTrp: 1.869 ± 0.306
3.399GlyTyr: 3.399 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
1.402HisAla: 1.402 ± 0.287
0.127HisCys: 0.127 ± 0.079
1.317HisAsp: 1.317 ± 0.25
1.19HisGlu: 1.19 ± 0.23
0.637HisPhe: 0.637 ± 0.151
1.827HisGly: 1.827 ± 0.388
0.467HisHis: 0.467 ± 0.131
0.977HisIle: 0.977 ± 0.173
0.85HisLys: 0.85 ± 0.181
1.869HisLeu: 1.869 ± 0.281
0.467HisMet: 0.467 ± 0.124
0.68HisAsn: 0.68 ± 0.213
1.147HisPro: 1.147 ± 0.227
0.722HisGln: 0.722 ± 0.193
1.444HisArg: 1.444 ± 0.237
1.444HisSer: 1.444 ± 0.251
1.19HisThr: 1.19 ± 0.265
1.784HisVal: 1.784 ± 0.264
0.68HisTrp: 0.68 ± 0.186
0.765HisTyr: 0.765 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.778IleAla: 5.778 ± 0.536
0.68IleCys: 0.68 ± 0.204
2.931IleAsp: 2.931 ± 0.402
3.229IleGlu: 3.229 ± 0.357
1.572IlePhe: 1.572 ± 0.256
4.036IleGly: 4.036 ± 0.413
0.935IleHis: 0.935 ± 0.165
1.869IleIle: 1.869 ± 0.33
1.487IleLys: 1.487 ± 0.313
2.804IleLeu: 2.804 ± 0.386
0.68IleMet: 0.68 ± 0.215
1.275IleAsn: 1.275 ± 0.216
2.761IlePro: 2.761 ± 0.417
1.997IleGln: 1.997 ± 0.313
3.186IleArg: 3.186 ± 0.381
2.252IleSer: 2.252 ± 0.365
2.719IleThr: 2.719 ± 0.246
2.974IleVal: 2.974 ± 0.318
0.637IleTrp: 0.637 ± 0.139
1.147IleTyr: 1.147 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
5.141LysAla: 5.141 ± 0.575
0.425LysCys: 0.425 ± 0.15
2.294LysAsp: 2.294 ± 0.332
1.997LysGlu: 1.997 ± 0.283
1.402LysPhe: 1.402 ± 0.227
2.549LysGly: 2.549 ± 0.368
1.19LysHis: 1.19 ± 0.209
1.275LysIle: 1.275 ± 0.193
2.804LysLys: 2.804 ± 0.49
4.971LysLeu: 4.971 ± 0.522
1.105LysMet: 1.105 ± 0.223
1.529LysAsn: 1.529 ± 0.278
2.761LysPro: 2.761 ± 0.444
1.232LysGln: 1.232 ± 0.213
2.464LysArg: 2.464 ± 0.365
2.337LysSer: 2.337 ± 0.295
2.167LysThr: 2.167 ± 0.365
3.569LysVal: 3.569 ± 0.435
0.765LysTrp: 0.765 ± 0.164
1.232LysTyr: 1.232 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
8.242LeuAla: 8.242 ± 0.629
0.595LeuCys: 0.595 ± 0.17
6.543LeuAsp: 6.543 ± 0.601
3.781LeuGlu: 3.781 ± 0.404
2.889LeuPhe: 2.889 ± 0.358
6.288LeuGly: 6.288 ± 0.594
1.699LeuHis: 1.699 ± 0.319
3.824LeuIle: 3.824 ± 0.515
3.484LeuLys: 3.484 ± 0.558
6.458LeuLeu: 6.458 ± 0.597
2.124LeuMet: 2.124 ± 0.285
2.846LeuAsn: 2.846 ± 0.383
4.418LeuPro: 4.418 ± 0.435
2.167LeuGln: 2.167 ± 0.282
5.438LeuArg: 5.438 ± 0.41
4.716LeuSer: 4.716 ± 0.575
4.928LeuThr: 4.928 ± 0.547
5.693LeuVal: 5.693 ± 0.483
1.614LeuTrp: 1.614 ± 0.265
1.827LeuTyr: 1.827 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
2.634MetAla: 2.634 ± 0.381
0.17MetCys: 0.17 ± 0.082
1.19MetAsp: 1.19 ± 0.226
1.232MetGlu: 1.232 ± 0.21
0.637MetPhe: 0.637 ± 0.169
2.167MetGly: 2.167 ± 0.299
0.382MetHis: 0.382 ± 0.119
0.892MetIle: 0.892 ± 0.17
1.147MetLys: 1.147 ± 0.216
1.614MetLeu: 1.614 ± 0.303
0.595MetMet: 0.595 ± 0.149
0.892MetAsn: 0.892 ± 0.195
1.827MetPro: 1.827 ± 0.244
0.595MetGln: 0.595 ± 0.174
1.784MetArg: 1.784 ± 0.261
2.422MetSer: 2.422 ± 0.309
2.422MetThr: 2.422 ± 0.265
1.232MetVal: 1.232 ± 0.221
0.467MetTrp: 0.467 ± 0.121
0.722MetTyr: 0.722 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
3.314AsnAla: 3.314 ± 0.453
0.212AsnCys: 0.212 ± 0.089
1.869AsnAsp: 1.869 ± 0.31
1.997AsnGlu: 1.997 ± 0.33
1.19AsnPhe: 1.19 ± 0.266
3.654AsnGly: 3.654 ± 0.385
0.51AsnHis: 0.51 ± 0.134
1.232AsnIle: 1.232 ± 0.17
1.232AsnLys: 1.232 ± 0.263
3.441AsnLeu: 3.441 ± 0.479
0.552AsnMet: 0.552 ± 0.168
0.722AsnAsn: 0.722 ± 0.195
2.677AsnPro: 2.677 ± 0.376
0.892AsnGln: 0.892 ± 0.194
2.167AsnArg: 2.167 ± 0.358
1.784AsnSer: 1.784 ± 0.292
1.742AsnThr: 1.742 ± 0.262
2.846AsnVal: 2.846 ± 0.444
0.467AsnTrp: 0.467 ± 0.124
0.977AsnTyr: 0.977 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
5.013ProAla: 5.013 ± 0.398
0.807ProCys: 0.807 ± 0.215
4.588ProAsp: 4.588 ± 0.601
4.163ProGlu: 4.163 ± 0.422
1.742ProPhe: 1.742 ± 0.275
3.781ProGly: 3.781 ± 0.553
0.977ProHis: 0.977 ± 0.224
2.167ProIle: 2.167 ± 0.341
2.677ProLys: 2.677 ± 0.438
3.399ProLeu: 3.399 ± 0.418
1.36ProMet: 1.36 ± 0.245
2.252ProAsn: 2.252 ± 0.322
1.997ProPro: 1.997 ± 0.337
1.699ProGln: 1.699 ± 0.253
2.422ProArg: 2.422 ± 0.33
3.271ProSer: 3.271 ± 0.376
3.696ProThr: 3.696 ± 0.45
3.739ProVal: 3.739 ± 0.518
1.614ProTrp: 1.614 ± 0.312
1.402ProTyr: 1.402 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
4.461GlnAla: 4.461 ± 0.412
0.17GlnCys: 0.17 ± 0.084
1.444GlnAsp: 1.444 ± 0.235
1.784GlnGlu: 1.784 ± 0.265
1.572GlnPhe: 1.572 ± 0.255
2.846GlnGly: 2.846 ± 0.491
0.807GlnHis: 0.807 ± 0.246
1.869GlnIle: 1.869 ± 0.241
1.657GlnLys: 1.657 ± 0.345
3.016GlnLeu: 3.016 ± 0.376
1.105GlnMet: 1.105 ± 0.219
1.105GlnAsn: 1.105 ± 0.182
1.742GlnPro: 1.742 ± 0.223
1.742GlnGln: 1.742 ± 0.278
2.082GlnArg: 2.082 ± 0.264
1.912GlnSer: 1.912 ± 0.249
1.912GlnThr: 1.912 ± 0.269
2.379GlnVal: 2.379 ± 0.314
0.467GlnTrp: 0.467 ± 0.147
0.935GlnTyr: 0.935 ± 0.173
0.0GlnXaa: 0.0 ± 0.0
Arg
5.65ArgAla: 5.65 ± 0.525
0.552ArgCys: 0.552 ± 0.171
4.206ArgAsp: 4.206 ± 0.437
4.588ArgGlu: 4.588 ± 0.479
2.549ArgPhe: 2.549 ± 0.364
4.503ArgGly: 4.503 ± 0.474
1.36ArgHis: 1.36 ± 0.262
3.101ArgIle: 3.101 ± 0.359
3.314ArgLys: 3.314 ± 0.343
5.226ArgLeu: 5.226 ± 0.486
1.402ArgMet: 1.402 ± 0.243
2.422ArgAsn: 2.422 ± 0.516
2.337ArgPro: 2.337 ± 0.351
2.846ArgGln: 2.846 ± 0.333
3.569ArgArg: 3.569 ± 0.391
3.144ArgSer: 3.144 ± 0.357
3.144ArgThr: 3.144 ± 0.411
4.673ArgVal: 4.673 ± 0.505
1.572ArgTrp: 1.572 ± 0.335
1.869ArgTyr: 1.869 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
6.118SerAla: 6.118 ± 0.484
0.51SerCys: 0.51 ± 0.168
4.376SerAsp: 4.376 ± 0.463
2.634SerGlu: 2.634 ± 0.305
1.912SerPhe: 1.912 ± 0.309
6.373SerGly: 6.373 ± 0.576
1.317SerHis: 1.317 ± 0.228
2.507SerIle: 2.507 ± 0.363
1.742SerLys: 1.742 ± 0.282
4.461SerLeu: 4.461 ± 0.46
1.827SerMet: 1.827 ± 0.26
2.209SerAsn: 2.209 ± 0.339
2.719SerPro: 2.719 ± 0.38
2.252SerGln: 2.252 ± 0.286
3.781SerArg: 3.781 ± 0.486
4.376SerSer: 4.376 ± 0.707
3.399SerThr: 3.399 ± 0.436
4.376SerVal: 4.376 ± 0.369
0.935SerTrp: 0.935 ± 0.176
1.699SerTyr: 1.699 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
5.48ThrAla: 5.48 ± 0.635
0.382ThrCys: 0.382 ± 0.136
4.079ThrAsp: 4.079 ± 0.359
3.441ThrGlu: 3.441 ± 0.452
2.294ThrPhe: 2.294 ± 0.345
6.118ThrGly: 6.118 ± 0.535
1.19ThrHis: 1.19 ± 0.271
2.974ThrIle: 2.974 ± 0.348
2.846ThrLys: 2.846 ± 0.283
4.588ThrLeu: 4.588 ± 0.382
0.935ThrMet: 0.935 ± 0.166
2.252ThrAsn: 2.252 ± 0.286
4.248ThrPro: 4.248 ± 0.449
1.402ThrGln: 1.402 ± 0.297
3.314ThrArg: 3.314 ± 0.426
3.059ThrSer: 3.059 ± 0.441
3.696ThrThr: 3.696 ± 0.404
4.886ThrVal: 4.886 ± 0.571
1.232ThrTrp: 1.232 ± 0.228
1.912ThrTyr: 1.912 ± 0.241
0.0ThrXaa: 0.0 ± 0.0
Val
7.86ValAla: 7.86 ± 0.698
1.062ValCys: 1.062 ± 0.228
6.203ValAsp: 6.203 ± 0.521
4.801ValGlu: 4.801 ± 0.498
2.634ValPhe: 2.634 ± 0.353
5.693ValGly: 5.693 ± 0.501
1.784ValHis: 1.784 ± 0.339
3.739ValIle: 3.739 ± 0.39
2.804ValLys: 2.804 ± 0.329
4.546ValLeu: 4.546 ± 0.439
1.232ValMet: 1.232 ± 0.211
1.954ValAsn: 1.954 ± 0.25
3.739ValPro: 3.739 ± 0.432
2.422ValGln: 2.422 ± 0.312
4.673ValArg: 4.673 ± 0.501
4.758ValSer: 4.758 ± 0.481
4.121ValThr: 4.121 ± 0.545
6.203ValVal: 6.203 ± 0.53
1.529ValTrp: 1.529 ± 0.279
2.082ValTyr: 2.082 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
2.379TrpAla: 2.379 ± 0.305
0.17TrpCys: 0.17 ± 0.079
1.147TrpAsp: 1.147 ± 0.179
0.935TrpGlu: 0.935 ± 0.214
0.68TrpPhe: 0.68 ± 0.176
1.742TrpGly: 1.742 ± 0.283
0.382TrpHis: 0.382 ± 0.129
1.19TrpIle: 1.19 ± 0.231
0.807TrpLys: 0.807 ± 0.158
1.954TrpLeu: 1.954 ± 0.292
0.595TrpMet: 0.595 ± 0.132
0.892TrpAsn: 0.892 ± 0.194
1.02TrpPro: 1.02 ± 0.229
0.977TrpGln: 0.977 ± 0.207
1.657TrpArg: 1.657 ± 0.283
1.147TrpSer: 1.147 ± 0.251
1.742TrpThr: 1.742 ± 0.286
1.275TrpVal: 1.275 ± 0.228
0.552TrpTrp: 0.552 ± 0.126
0.382TrpTyr: 0.382 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.144TyrAla: 3.144 ± 0.328
0.127TyrCys: 0.127 ± 0.077
1.912TyrAsp: 1.912 ± 0.334
1.912TyrGlu: 1.912 ± 0.342
1.02TyrPhe: 1.02 ± 0.186
3.314TyrGly: 3.314 ± 0.396
0.595TyrHis: 0.595 ± 0.17
0.595TyrIle: 0.595 ± 0.143
1.02TyrLys: 1.02 ± 0.201
2.464TyrLeu: 2.464 ± 0.308
0.467TyrMet: 0.467 ± 0.143
1.147TyrAsn: 1.147 ± 0.22
1.147TyrPro: 1.147 ± 0.237
1.147TyrGln: 1.147 ± 0.217
1.912TyrArg: 1.912 ± 0.292
1.529TyrSer: 1.529 ± 0.264
1.827TyrThr: 1.827 ± 0.26
2.039TyrVal: 2.039 ± 0.328
0.637TyrTrp: 0.637 ± 0.154
0.68TyrTyr: 0.68 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 129 proteins (23539 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski