Stenotrophomonas phage SMA9

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Staminivirus; Stenotrophomonas virus SMA9

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4LAU3|Q4LAU3_9VIRU Uncharacterized protein OS=Stenotrophomonas phage SMA9 OX=334856 PE=4 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84LALLMLATAALMLSPRR19 pKa = 11.84VLAADD24 pKa = 4.81ASCSTSPSVLEE35 pKa = 4.34SSCPDD40 pKa = 2.97EE41 pKa = 4.58GLAYY45 pKa = 10.04AAAWATANAQAAKK58 pKa = 10.46SNTGSSGWCAGVKK71 pKa = 9.73KK72 pKa = 10.53QGVGSFLAYY81 pKa = 7.82VTPCSSPGPAYY92 pKa = 7.67EE93 pKa = 3.79TRR95 pKa = 11.84TRR97 pKa = 11.84SFSALCSARR106 pKa = 11.84AEE108 pKa = 4.13EE109 pKa = 4.87FGWQGGEE116 pKa = 3.77TAASVNACHH125 pKa = 6.51NGCMYY130 pKa = 10.45TSSLDD135 pKa = 3.65PKK137 pKa = 9.04GTAGVSFTPTGGTCTEE153 pKa = 4.42ADD155 pKa = 3.64APAPTPAGDD164 pKa = 3.92GGDD167 pKa = 3.66PGEE170 pKa = 4.8GGGGDD175 pKa = 3.88GGGNDD180 pKa = 4.15PGGGDD185 pKa = 3.31GGGDD189 pKa = 3.22NGGGDD194 pKa = 3.97GDD196 pKa = 4.19GGGNNPGGGDD206 pKa = 3.51GGSGGGDD213 pKa = 3.22GDD215 pKa = 4.49GDD217 pKa = 4.06GDD219 pKa = 4.55GDD221 pKa = 4.55GDD223 pKa = 4.12GDD225 pKa = 4.32GDD227 pKa = 4.15GGGPGPGPGPGEE239 pKa = 4.18GDD241 pKa = 3.01GDD243 pKa = 4.18GPGEE247 pKa = 4.22VGSDD251 pKa = 3.29GGPLYY256 pKa = 10.86EE257 pKa = 4.6RR258 pKa = 11.84DD259 pKa = 4.0KK260 pKa = 11.65NLTLDD265 pKa = 4.08KK266 pKa = 11.01VFNDD270 pKa = 4.57FKK272 pKa = 11.13QQAEE276 pKa = 4.41KK277 pKa = 11.05LPIIQATKK285 pKa = 10.46SFFTVSVGGSCPVFTLPASQYY306 pKa = 8.54WDD308 pKa = 2.86TMTFDD313 pKa = 3.4LHH315 pKa = 7.4CYY317 pKa = 8.76GVIYY321 pKa = 10.34EE322 pKa = 4.63SFLLMGWVLLAIAAFMAAKK341 pKa = 10.12IALTT345 pKa = 3.68

Molecular weight: 33.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4LAU4|Q4LAU4_9VIRU Putative zonula occludens toxin OS=Stenotrophomonas phage SMA9 OX=334856 GN=zot PE=4 SV=1
MM1 pKa = 7.21MNFLAFLMPHH11 pKa = 6.79AGWLTDD17 pKa = 3.43LTQWLLRR24 pKa = 11.84QIQRR28 pKa = 11.84FWDD31 pKa = 3.45ALVAFFNDD39 pKa = 3.73LLILAIQTMLALMIKK54 pKa = 9.71MIGALPVPDD63 pKa = 3.53VLKK66 pKa = 10.6NYY68 pKa = 10.66SIGTLLGNAGSTVGWFVQTFKK89 pKa = 11.14LGEE92 pKa = 4.18CLALIGSAVAFRR104 pKa = 11.84ILRR107 pKa = 11.84KK108 pKa = 10.17VMTMGKK114 pKa = 7.64WW115 pKa = 2.89

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1631

92

442

233.0

25.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.668 ± 0.841

1.778 ± 0.397

5.886 ± 0.613

4.905 ± 0.762

3.74 ± 0.402

11.772 ± 2.047

1.717 ± 0.4

3.924 ± 0.6

4.414 ± 0.492

8.645 ± 1.001

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.529

3.004 ± 0.25

5.641 ± 0.805

3.311 ± 0.469

5.947 ± 1.276

5.518 ± 0.902

4.537 ± 0.768

7.48 ± 0.991

2.023 ± 0.481

2.575 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski