Amino acid dipepetide frequency for Stenotrophomonas phage SMA9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.11AlaAla: 14.11 ± 2.92
1.84AlaCys: 1.84 ± 0.789
2.454AlaAsp: 2.454 ± 1.026
6.135AlaGlu: 6.135 ± 1.374
4.294AlaPhe: 4.294 ± 2.03
10.429AlaGly: 10.429 ± 1.733
0.613AlaHis: 0.613 ± 0.524
3.067AlaIle: 3.067 ± 0.936
5.521AlaLys: 5.521 ± 2.181
15.337AlaLeu: 15.337 ± 3.106
3.067AlaMet: 3.067 ± 1.608
1.227AlaAsn: 1.227 ± 0.513
3.681AlaPro: 3.681 ± 1.677
4.294AlaGln: 4.294 ± 0.959
4.294AlaArg: 4.294 ± 1.163
2.454AlaSer: 2.454 ± 1.733
5.521AlaThr: 5.521 ± 1.66
12.27AlaVal: 12.27 ± 3.783
2.454AlaTrp: 2.454 ± 1.64
3.067AlaTyr: 3.067 ± 1.4
0.0AlaXaa: 0.0 ± 0.0
Cys
1.84CysAla: 1.84 ± 1.1
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.613CysHis: 0.613 ± 0.551
0.613CysIle: 0.613 ± 0.49
1.227CysLys: 1.227 ± 1.048
3.067CysLeu: 3.067 ± 1.413
0.613CysMet: 0.613 ± 0.551
0.0CysAsn: 0.0 ± 0.0
1.84CysPro: 1.84 ± 1.139
0.613CysGln: 0.613 ± 0.864
1.227CysArg: 1.227 ± 0.878
1.84CysSer: 1.84 ± 1.653
1.84CysThr: 1.84 ± 1.1
1.227CysVal: 1.227 ± 0.598
0.0CysTrp: 0.0 ± 0.0
1.227CysTyr: 1.227 ± 0.513
0.0CysXaa: 0.0 ± 0.0
Asp
7.362AspAla: 7.362 ± 1.892
0.613AspCys: 0.613 ± 0.524
1.84AspAsp: 1.84 ± 0.96
3.067AspGlu: 3.067 ± 1.083
1.84AspPhe: 1.84 ± 0.761
11.043AspGly: 11.043 ± 9.296
0.0AspHis: 0.0 ± 0.0
1.84AspIle: 1.84 ± 1.415
1.84AspLys: 1.84 ± 0.945
6.748AspLeu: 6.748 ± 3.183
0.0AspMet: 0.0 ± 0.0
1.84AspAsn: 1.84 ± 0.695
4.908AspPro: 4.908 ± 1.406
1.84AspGln: 1.84 ± 1.471
3.067AspArg: 3.067 ± 1.613
3.067AspSer: 3.067 ± 1.039
1.227AspThr: 1.227 ± 0.513
4.908AspVal: 4.908 ± 1.286
0.613AspTrp: 0.613 ± 0.524
1.227AspTyr: 1.227 ± 1.048
0.0AspXaa: 0.0 ± 0.0
Glu
2.454GluAla: 2.454 ± 0.632
0.613GluCys: 0.613 ± 0.755
1.84GluAsp: 1.84 ± 1.085
4.908GluGlu: 4.908 ± 1.615
1.84GluPhe: 1.84 ± 0.789
6.748GluGly: 6.748 ± 2.107
0.0GluHis: 0.0 ± 0.0
1.84GluIle: 1.84 ± 1.071
4.908GluLys: 4.908 ± 1.342
2.454GluLeu: 2.454 ± 1.305
0.613GluMet: 0.613 ± 0.634
1.84GluAsn: 1.84 ± 0.96
1.227GluPro: 1.227 ± 0.882
1.227GluGln: 1.227 ± 0.598
5.521GluArg: 5.521 ± 1.646
1.84GluSer: 1.84 ± 0.945
1.227GluThr: 1.227 ± 1.102
5.521GluVal: 5.521 ± 2.85
1.227GluTrp: 1.227 ± 0.877
1.227GluTyr: 1.227 ± 0.752
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 1.567
0.613PheCys: 0.613 ± 0.634
1.84PheAsp: 1.84 ± 0.789
0.613PheGlu: 0.613 ± 0.586
2.454PhePhe: 2.454 ± 1.017
3.067PheGly: 3.067 ± 0.916
0.613PheHis: 0.613 ± 0.49
1.227PheIle: 1.227 ± 0.598
1.84PheLys: 1.84 ± 0.821
4.908PheLeu: 4.908 ± 1.491
1.227PheMet: 1.227 ± 0.541
1.84PheAsn: 1.84 ± 0.821
0.613PhePro: 0.613 ± 0.49
0.613PheGln: 0.613 ± 0.49
4.294PheArg: 4.294 ± 1.92
1.84PheSer: 1.84 ± 0.903
3.067PheThr: 3.067 ± 1.803
1.84PheVal: 1.84 ± 1.412
1.227PheTrp: 1.227 ± 0.855
1.227PheTyr: 1.227 ± 0.598
0.0PheXaa: 0.0 ± 0.0
Gly
7.975GlyAla: 7.975 ± 2.633
2.454GlyCys: 2.454 ± 1.577
14.724GlyAsp: 14.724 ± 9.028
6.748GlyGlu: 6.748 ± 2.224
4.294GlyPhe: 4.294 ± 1.511
24.54GlyGly: 24.54 ± 11.602
1.84GlyHis: 1.84 ± 1.572
2.454GlyIle: 2.454 ± 1.196
5.521GlyLys: 5.521 ± 1.847
4.294GlyLeu: 4.294 ± 1.154
2.454GlyMet: 2.454 ± 1.402
4.294GlyAsn: 4.294 ± 1.414
7.362GlyPro: 7.362 ± 4.042
2.454GlyGln: 2.454 ± 1.196
3.067GlyArg: 3.067 ± 1.428
7.975GlySer: 7.975 ± 2.463
4.294GlyThr: 4.294 ± 1.704
7.362GlyVal: 7.362 ± 2.222
3.067GlyTrp: 3.067 ± 2.128
0.613GlyTyr: 0.613 ± 0.49
0.0GlyXaa: 0.0 ± 0.0
His
1.227HisAla: 1.227 ± 0.806
0.613HisCys: 0.613 ± 0.551
1.84HisAsp: 1.84 ± 1.236
1.227HisGlu: 1.227 ± 0.98
0.0HisPhe: 0.0 ± 0.0
1.84HisGly: 1.84 ± 0.96
0.613HisHis: 0.613 ± 0.864
0.613HisIle: 0.613 ± 0.49
0.613HisLys: 0.613 ± 0.524
0.0HisLeu: 0.0 ± 0.0
0.613HisMet: 0.613 ± 0.524
1.84HisAsn: 1.84 ± 0.761
0.613HisPro: 0.613 ± 0.524
0.613HisGln: 0.613 ± 0.49
1.84HisArg: 1.84 ± 1.166
0.0HisSer: 0.0 ± 0.0
0.613HisThr: 0.613 ± 0.49
1.227HisVal: 1.227 ± 0.707
0.0HisTrp: 0.0 ± 0.0
1.227HisTyr: 1.227 ± 1.268
0.0HisXaa: 0.0 ± 0.0
Ile
4.908IleAla: 4.908 ± 1.245
0.613IleCys: 0.613 ± 0.586
3.681IleAsp: 3.681 ± 1.765
1.84IleGlu: 1.84 ± 1.085
0.613IlePhe: 0.613 ± 0.524
4.908IleGly: 4.908 ± 2.101
1.227IleHis: 1.227 ± 1.268
1.84IleIle: 1.84 ± 0.761
1.84IleLys: 1.84 ± 0.809
3.067IleLeu: 3.067 ± 1.911
0.613IleMet: 0.613 ± 0.49
0.613IleAsn: 0.613 ± 0.49
1.84IlePro: 1.84 ± 0.96
2.454IleGln: 2.454 ± 1.612
3.681IleArg: 3.681 ± 1.429
1.227IleSer: 1.227 ± 0.598
1.227IleThr: 1.227 ± 0.882
1.227IleVal: 1.227 ± 0.598
0.613IleTrp: 0.613 ± 0.634
1.227IleTyr: 1.227 ± 0.513
0.0IleXaa: 0.0 ± 0.0
Lys
3.681LysAla: 3.681 ± 1.426
0.0LysCys: 0.0 ± 0.0
2.454LysAsp: 2.454 ± 1.466
1.227LysGlu: 1.227 ± 0.598
0.0LysPhe: 0.0 ± 0.0
6.135LysGly: 6.135 ± 2.846
1.84LysHis: 1.84 ± 1.071
2.454LysIle: 2.454 ± 1.284
2.454LysLys: 2.454 ± 0.924
3.681LysLeu: 3.681 ± 0.718
0.613LysMet: 0.613 ± 0.755
4.294LysAsn: 4.294 ± 1.03
1.227LysPro: 1.227 ± 0.98
3.681LysGln: 3.681 ± 0.953
1.227LysArg: 1.227 ± 1.131
4.294LysSer: 4.294 ± 0.785
1.227LysThr: 1.227 ± 0.752
1.227LysVal: 1.227 ± 0.891
1.227LysTrp: 1.227 ± 0.806
1.227LysTyr: 1.227 ± 0.98
0.0LysXaa: 0.0 ± 0.0
Leu
9.816LeuAla: 9.816 ± 3.588
3.067LeuCys: 3.067 ± 0.848
4.908LeuAsp: 4.908 ± 1.879
1.227LeuGlu: 1.227 ± 0.694
1.84LeuPhe: 1.84 ± 1.236
6.748LeuGly: 6.748 ± 3.087
3.067LeuHis: 3.067 ± 0.848
4.294LeuIle: 4.294 ± 1.727
1.84LeuLys: 1.84 ± 1.038
7.975LeuLeu: 7.975 ± 2.173
3.067LeuMet: 3.067 ± 2.128
2.454LeuAsn: 2.454 ± 1.196
7.975LeuPro: 7.975 ± 2.521
0.613LeuGln: 0.613 ± 0.524
4.294LeuArg: 4.294 ± 1.908
6.748LeuSer: 6.748 ± 2.207
7.975LeuThr: 7.975 ± 1.872
4.294LeuVal: 4.294 ± 2.541
1.227LeuTrp: 1.227 ± 1.268
3.067LeuTyr: 3.067 ± 0.99
0.0LeuXaa: 0.0 ± 0.0
Met
4.294MetAla: 4.294 ± 2.039
0.0MetCys: 0.0 ± 0.0
0.613MetAsp: 0.613 ± 0.49
1.227MetGlu: 1.227 ± 0.882
0.0MetPhe: 0.0 ± 0.0
3.067MetGly: 3.067 ± 0.839
0.0MetHis: 0.0 ± 0.0
1.84MetIle: 1.84 ± 1.798
0.613MetLys: 0.613 ± 0.49
3.067MetLeu: 3.067 ± 0.976
1.227MetMet: 1.227 ± 1.192
0.613MetAsn: 0.613 ± 0.755
2.454MetPro: 2.454 ± 1.566
0.613MetGln: 0.613 ± 0.49
0.0MetArg: 0.0 ± 0.0
1.227MetSer: 1.227 ± 0.814
1.84MetThr: 1.84 ± 0.821
1.227MetVal: 1.227 ± 0.752
0.613MetTrp: 0.613 ± 0.634
0.613MetTyr: 0.613 ± 0.551
0.0MetXaa: 0.0 ± 0.0
Asn
3.681AsnAla: 3.681 ± 2.169
0.613AsnCys: 0.613 ± 0.524
1.84AsnAsp: 1.84 ± 1.274
1.227AsnGlu: 1.227 ± 0.598
1.84AsnPhe: 1.84 ± 0.809
3.067AsnGly: 3.067 ± 1.028
1.227AsnHis: 1.227 ± 0.98
0.613AsnIle: 0.613 ± 0.49
0.0AsnLys: 0.0 ± 0.0
2.454AsnLeu: 2.454 ± 1.573
0.0AsnMet: 0.0 ± 0.0
1.227AsnAsn: 1.227 ± 0.513
1.227AsnPro: 1.227 ± 0.694
0.0AsnGln: 0.0 ± 0.0
1.84AsnArg: 1.84 ± 1.471
1.84AsnSer: 1.84 ± 1.351
3.067AsnThr: 3.067 ± 0.99
1.84AsnVal: 1.84 ± 0.96
1.227AsnTrp: 1.227 ± 1.048
1.227AsnTyr: 1.227 ± 0.855
0.0AsnXaa: 0.0 ± 0.0
Pro
6.748ProAla: 6.748 ± 2.573
0.613ProCys: 0.613 ± 0.551
4.294ProAsp: 4.294 ± 1.733
3.681ProGlu: 3.681 ± 2.028
1.227ProPhe: 1.227 ± 0.723
9.816ProGly: 9.816 ± 4.328
1.84ProHis: 1.84 ± 1.369
1.84ProIle: 1.84 ± 1.123
1.227ProLys: 1.227 ± 0.513
4.908ProLeu: 4.908 ± 1.798
1.84ProMet: 1.84 ± 1.127
0.613ProAsn: 0.613 ± 0.49
3.067ProPro: 3.067 ± 1.473
1.84ProGln: 1.84 ± 1.415
3.681ProArg: 3.681 ± 1.004
2.454ProSer: 2.454 ± 0.632
2.454ProThr: 2.454 ± 1.026
1.84ProVal: 1.84 ± 1.002
1.227ProTrp: 1.227 ± 0.877
1.227ProTyr: 1.227 ± 0.752
0.0ProXaa: 0.0 ± 0.0
Gln
4.908GlnAla: 4.908 ± 1.701
0.613GlnCys: 0.613 ± 0.524
1.227GlnAsp: 1.227 ± 1.048
0.613GlnGlu: 0.613 ± 0.864
0.613GlnPhe: 0.613 ± 0.49
3.681GlnGly: 3.681 ± 1.055
0.0GlnHis: 0.0 ± 0.0
0.613GlnIle: 0.613 ± 0.755
2.454GlnLys: 2.454 ± 1.155
2.454GlnLeu: 2.454 ± 1.961
0.613GlnMet: 0.613 ± 0.586
0.613GlnAsn: 0.613 ± 0.49
0.613GlnPro: 0.613 ± 0.49
1.84GlnGln: 1.84 ± 0.533
3.067GlnArg: 3.067 ± 1.921
3.067GlnSer: 3.067 ± 1.271
1.227GlnThr: 1.227 ± 1.51
1.227GlnVal: 1.227 ± 0.98
2.454GlnTrp: 2.454 ± 1.186
1.227GlnTyr: 1.227 ± 0.703
0.0GlnXaa: 0.0 ± 0.0
Arg
4.294ArgAla: 4.294 ± 1.221
0.0ArgCys: 0.0 ± 0.0
2.454ArgAsp: 2.454 ± 1.14
3.681ArgGlu: 3.681 ± 1.887
2.454ArgPhe: 2.454 ± 1.568
2.454ArgGly: 2.454 ± 1.578
1.227ArgHis: 1.227 ± 0.723
5.521ArgIle: 5.521 ± 2.013
2.454ArgLys: 2.454 ± 1.021
4.908ArgLeu: 4.908 ± 1.258
1.84ArgMet: 1.84 ± 1.071
0.0ArgAsn: 0.0 ± 0.0
2.454ArgPro: 2.454 ± 1.31
3.681ArgGln: 3.681 ± 1.433
4.294ArgArg: 4.294 ± 1.956
3.067ArgSer: 3.067 ± 2.048
1.227ArgThr: 1.227 ± 0.694
9.816ArgVal: 9.816 ± 3.269
1.227ArgTrp: 1.227 ± 0.707
2.454ArgTyr: 2.454 ± 1.066
0.0ArgXaa: 0.0 ± 0.0
Ser
9.202SerAla: 9.202 ± 2.289
2.454SerCys: 2.454 ± 1.733
1.84SerAsp: 1.84 ± 0.814
1.227SerGlu: 1.227 ± 0.71
5.521SerPhe: 5.521 ± 2.272
1.84SerGly: 1.84 ± 0.945
0.0SerHis: 0.0 ± 0.0
1.84SerIle: 1.84 ± 1.404
3.067SerLys: 3.067 ± 1.653
3.067SerLeu: 3.067 ± 0.766
0.0SerMet: 0.0 ± 0.0
2.454SerAsn: 2.454 ± 0.571
4.908SerPro: 4.908 ± 1.642
1.84SerGln: 1.84 ± 1.171
4.294SerArg: 4.294 ± 1.626
4.294SerSer: 4.294 ± 1.821
3.067SerThr: 3.067 ± 0.839
2.454SerVal: 2.454 ± 1.724
0.0SerTrp: 0.0 ± 0.0
1.84SerTyr: 1.84 ± 0.666
0.0SerXaa: 0.0 ± 0.0
Thr
6.135ThrAla: 6.135 ± 2.057
0.613ThrCys: 0.613 ± 0.551
1.227ThrAsp: 1.227 ± 0.806
2.454ThrGlu: 2.454 ± 0.753
3.681ThrPhe: 3.681 ± 1.788
4.908ThrGly: 4.908 ± 1.115
0.0ThrHis: 0.0 ± 0.0
2.454ThrIle: 2.454 ± 1.196
1.227ThrLys: 1.227 ± 0.513
4.294ThrLeu: 4.294 ± 1.131
2.454ThrMet: 2.454 ± 1.788
1.227ThrAsn: 1.227 ± 0.752
3.681ThrPro: 3.681 ± 1.346
1.227ThrGln: 1.227 ± 0.855
2.454ThrArg: 2.454 ± 1.388
1.84ThrSer: 1.84 ± 0.945
0.613ThrThr: 0.613 ± 0.49
4.294ThrVal: 4.294 ± 1.309
0.613ThrTrp: 0.613 ± 0.864
0.0ThrTyr: 0.0 ± 0.0
0.0ThrXaa: 0.0 ± 0.0
Val
6.748ValAla: 6.748 ± 2.432
1.227ValCys: 1.227 ± 1.048
5.521ValAsp: 5.521 ± 1.735
3.067ValGlu: 3.067 ± 1.245
4.294ValPhe: 4.294 ± 2.101
11.043ValGly: 11.043 ± 2.014
1.84ValHis: 1.84 ± 0.96
2.454ValIle: 2.454 ± 1.275
1.84ValLys: 1.84 ± 0.661
4.294ValLeu: 4.294 ± 2.02
3.067ValMet: 3.067 ± 1.641
2.454ValAsn: 2.454 ± 1.14
4.294ValPro: 4.294 ± 1.568
2.454ValGln: 2.454 ± 1.371
4.908ValArg: 4.908 ± 2.392
4.294ValSer: 4.294 ± 1.362
3.067ValThr: 3.067 ± 1.243
5.521ValVal: 5.521 ± 2.259
0.613ValTrp: 0.613 ± 0.49
1.84ValTyr: 1.84 ± 1.012
0.0ValXaa: 0.0 ± 0.0
Trp
3.067TrpAla: 3.067 ± 0.766
1.227TrpCys: 1.227 ± 0.513
1.84TrpAsp: 1.84 ± 1.214
0.0TrpGlu: 0.0 ± 0.0
0.613TrpPhe: 0.613 ± 0.755
0.0TrpGly: 0.0 ± 0.0
0.0TrpHis: 0.0 ± 0.0
1.227TrpIle: 1.227 ± 0.598
1.84TrpLys: 1.84 ± 1.254
3.681TrpLeu: 3.681 ± 2.013
0.613TrpMet: 0.613 ± 0.68
0.613TrpAsn: 0.613 ± 0.524
0.613TrpPro: 0.613 ± 0.49
1.227TrpGln: 1.227 ± 0.814
1.227TrpArg: 1.227 ± 1.137
0.613TrpSer: 0.613 ± 0.634
0.0TrpThr: 0.0 ± 0.0
1.84TrpVal: 1.84 ± 0.533
0.0TrpTrp: 0.0 ± 0.0
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.84TyrAla: 1.84 ± 0.695
0.0TyrCys: 0.0 ± 0.0
2.454TyrAsp: 2.454 ± 1.192
4.908TyrGlu: 4.908 ± 1.634
0.0TyrPhe: 0.0 ± 0.0
2.454TyrGly: 2.454 ± 0.603
0.613TyrHis: 0.613 ± 0.49
0.613TyrIle: 0.613 ± 0.634
1.84TyrLys: 1.84 ± 0.96
1.84TyrLeu: 1.84 ± 1.012
0.0TyrMet: 0.0 ± 0.0
0.0TyrAsn: 0.0 ± 0.0
1.84TyrPro: 1.84 ± 1.127
0.0TyrGln: 0.0 ± 0.0
1.227TyrArg: 1.227 ± 0.98
1.227TyrSer: 1.227 ± 0.806
0.613TyrThr: 0.613 ± 0.551
3.681TyrVal: 3.681 ± 0.974
0.613TyrTrp: 0.613 ± 0.551
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7 proteins (1631 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski