Novosphingobium sp. Rr 2-17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I9WLG0|I9WLG0_9SPHN RNA 3'-terminal phosphate cyclase OS=Novosphingobium sp. Rr 2-17 OX=555793 GN=rtcA PE=3 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84STHH5 pKa = 5.44QDD7 pKa = 2.66ASVRR11 pKa = 11.84LYY13 pKa = 10.78HH14 pKa = 7.32LDD16 pKa = 3.7GGPEE20 pKa = 3.87GGMATTLFYY29 pKa = 11.29GPLAQAMMIAAAEE42 pKa = 4.06PEE44 pKa = 4.32EE45 pKa = 4.18VQEE48 pKa = 4.39GLFLATDD55 pKa = 3.86NDD57 pKa = 3.68VVAYY61 pKa = 10.78LDD63 pKa = 4.43LLDD66 pKa = 4.06GG67 pKa = 4.45

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I9CB11|I9CB11_9SPHN Glutamate 5-kinase OS=Novosphingobium sp. Rr 2-17 OX=555793 GN=proB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84GATVGGRR28 pKa = 11.84KK29 pKa = 8.2VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4301

0

4301

1333011

22

2078

309.9

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.946 ± 0.056

0.818 ± 0.011

5.813 ± 0.029

5.217 ± 0.033

3.496 ± 0.019

8.727 ± 0.043

2.042 ± 0.018

4.96 ± 0.027

3.117 ± 0.032

9.947 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.017

2.639 ± 0.027

5.295 ± 0.031

3.318 ± 0.022

7.078 ± 0.041

5.628 ± 0.032

5.534 ± 0.031

7.284 ± 0.025

1.426 ± 0.016

2.345 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski