Daldinia sp. EC12

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Hypoxylaceae; Daldinia; unclassified Daldinia

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2WLD1|A0A1Y2WLD1_9PEZI FtsJ domain-containing protein OS=Daldinia sp. EC12 OX=1001832 GN=K445DRAFT_71247 PE=3 SV=1
MM1 pKa = 7.49SIKK4 pKa = 10.53SVFGISLLLGGSLARR19 pKa = 11.84DD20 pKa = 3.61VPSNIRR26 pKa = 11.84NFYY29 pKa = 11.36NNIKK33 pKa = 10.99NNGQCNSPLASGFYY47 pKa = 10.24SQYY50 pKa = 11.53DD51 pKa = 3.59DD52 pKa = 5.33DD53 pKa = 6.16NSFAYY58 pKa = 10.58CGDD61 pKa = 3.67HH62 pKa = 7.67LEE64 pKa = 4.43DD65 pKa = 3.72SGVIFLQGSNGQFANMDD82 pKa = 3.59IDD84 pKa = 4.49CDD86 pKa = 3.98GTQGGPADD94 pKa = 5.24DD95 pKa = 4.44GRR97 pKa = 11.84CGNSDD102 pKa = 3.6DD103 pKa = 4.27TQSITSFADD112 pKa = 3.37TVKK115 pKa = 10.93GYY117 pKa = 11.34GKK119 pKa = 10.82GVDD122 pKa = 4.15DD123 pKa = 5.87LDD125 pKa = 4.96AKK127 pKa = 9.59IHH129 pKa = 6.26PYY131 pKa = 10.47VVFGNVGDD139 pKa = 4.09VPGFTSFDD147 pKa = 3.78PQEE150 pKa = 4.59HH151 pKa = 6.39GIEE154 pKa = 4.29PLSVMAVVCGDD165 pKa = 3.4KK166 pKa = 10.75LIYY169 pKa = 10.0GVWGDD174 pKa = 3.69EE175 pKa = 4.08NGVDD179 pKa = 3.91GEE181 pKa = 4.3KK182 pKa = 10.63SVVGEE187 pKa = 4.16ASISLATACFGKK199 pKa = 10.89SMTGNNGHH207 pKa = 7.2DD208 pKa = 4.16EE209 pKa = 4.15NDD211 pKa = 3.19VLYY214 pKa = 10.34IAFTGGDD221 pKa = 3.94AVPGADD227 pKa = 3.33GAKK230 pKa = 9.37WDD232 pKa = 3.71AQNYY236 pKa = 9.22EE237 pKa = 4.31EE238 pKa = 5.14FEE240 pKa = 4.56SSIQDD245 pKa = 4.2LGDD248 pKa = 3.38QLIQRR253 pKa = 11.84IQAA256 pKa = 3.44

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2WVU2|A0A1Y2WVU2_9PEZI FAD-binding PCMH-type domain-containing protein OS=Daldinia sp. EC12 OX=1001832 GN=K445DRAFT_76648 PE=3 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84SFALAALPRR11 pKa = 11.84ARR13 pKa = 11.84GASKK17 pKa = 10.83NVLTTASASIQSLSMRR33 pKa = 11.84RR34 pKa = 11.84AFSSLPTLRR43 pKa = 11.84PTIQASTSSVFRR55 pKa = 11.84SPNPNNGLLQSFAPQPTGATGGATLDD81 pKa = 4.02LVPKK85 pKa = 9.87TSLTAHH91 pKa = 7.18PSLSGAAQVRR101 pKa = 11.84FGPRR105 pKa = 11.84PTMARR110 pKa = 11.84SSRR113 pKa = 11.84LVRR116 pKa = 11.84KK117 pKa = 9.31RR118 pKa = 11.84RR119 pKa = 11.84HH120 pKa = 5.32GFLSRR125 pKa = 11.84VRR127 pKa = 11.84THH129 pKa = 6.56NGRR132 pKa = 11.84KK133 pKa = 6.48TLQRR137 pKa = 11.84RR138 pKa = 11.84KK139 pKa = 8.33EE140 pKa = 4.02KK141 pKa = 10.32KK142 pKa = 10.01RR143 pKa = 11.84SVLSQQ148 pKa = 2.98

Molecular weight: 16.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11156

0

11156

5199705

50

4947

466.1

51.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.264 ± 0.023

1.205 ± 0.01

5.755 ± 0.017

6.242 ± 0.022

3.761 ± 0.014

6.825 ± 0.02

2.303 ± 0.009

5.197 ± 0.015

5.028 ± 0.021

8.818 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.009

3.886 ± 0.012

6.087 ± 0.025

3.867 ± 0.015

6.097 ± 0.02

8.185 ± 0.027

5.94 ± 0.015

6.075 ± 0.017

1.486 ± 0.009

2.908 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski