Symphysodon discus adomavirus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA viruses; Adomaviridae

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1MK48|A0A2S1MK48_9VIRU LO8 OS=Symphysodon discus adomavirus 1 OX=2175118 PE=4 SV=1
MM1 pKa = 6.94ATSRR5 pKa = 11.84QVQSLGRR12 pKa = 11.84TPGGMGYY19 pKa = 10.26SFLGTFDD26 pKa = 6.3DD27 pKa = 4.23NDD29 pKa = 3.43QNNYY33 pKa = 9.74CVLDD37 pKa = 4.38LSAHH41 pKa = 5.84PVACEE46 pKa = 3.41RR47 pKa = 11.84DD48 pKa = 3.61PYY50 pKa = 11.5GNGLAAISVTQITVSNCALHH70 pKa = 6.77IGNHH74 pKa = 6.1LGNHH78 pKa = 6.25YY79 pKa = 10.06IYY81 pKa = 11.03VGGARR86 pKa = 11.84VQLPDD91 pKa = 3.32VSNTSVTQFLDD102 pKa = 3.35YY103 pKa = 11.05LQACNPSLSFEE114 pKa = 4.32FKK116 pKa = 10.54LGRR119 pKa = 11.84LTYY122 pKa = 8.8TGNSAVIIPVRR133 pKa = 11.84TGIGQHH139 pKa = 6.26AKK141 pKa = 10.17PIPHH145 pKa = 7.09NLTHH149 pKa = 6.3MLGFTGLGEE158 pKa = 4.18VEE160 pKa = 4.86YY161 pKa = 10.26EE162 pKa = 3.64NDD164 pKa = 3.32MCVVITPDD172 pKa = 3.22VLGNSAPDD180 pKa = 3.24LFGPVRR186 pKa = 11.84DD187 pKa = 4.26LVVSCAEE194 pKa = 3.75VTYY197 pKa = 10.63NRR199 pKa = 11.84HH200 pKa = 4.94SRR202 pKa = 11.84QSVAVCSPCGTPGQAYY218 pKa = 8.22TRR220 pKa = 11.84DD221 pKa = 3.6CSDD224 pKa = 4.04LVRR227 pKa = 11.84EE228 pKa = 4.12LNIPGGYY235 pKa = 9.71LCSLTFTLTDD245 pKa = 3.38AADD248 pKa = 3.7RR249 pKa = 11.84PVRR252 pKa = 11.84FVHH255 pKa = 6.55GVPLICVRR263 pKa = 11.84ILPITLQQ270 pKa = 3.33

Molecular weight: 29.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1MK68|A0A2S1MK68_9VIRU EO4 OS=Symphysodon discus adomavirus 1 OX=2175118 PE=4 SV=1
MM1 pKa = 7.77AKK3 pKa = 9.01TRR5 pKa = 11.84LLPSKK10 pKa = 10.64KK11 pKa = 9.44KK12 pKa = 10.52GKK14 pKa = 9.69KK15 pKa = 7.4HH16 pKa = 5.39TPNKK20 pKa = 9.94VKK22 pKa = 10.25IKK24 pKa = 10.38KK25 pKa = 8.79EE26 pKa = 3.98PKK28 pKa = 9.99SGITALRR35 pKa = 11.84SYY37 pKa = 11.16KK38 pKa = 10.52HH39 pKa = 5.53FTRR42 pKa = 11.84RR43 pKa = 11.84WNKK46 pKa = 10.25KK47 pKa = 5.85NTQEE51 pKa = 4.29KK52 pKa = 9.9SSPVQTLVKK61 pKa = 10.46VEE63 pKa = 4.34NNIQSSHH70 pKa = 5.73TGGKK74 pKa = 8.78QRR76 pKa = 11.84STMPKK81 pKa = 9.49LRR83 pKa = 11.84AKK85 pKa = 10.21PVVKK89 pKa = 10.26KK90 pKa = 8.89RR91 pKa = 11.84TRR93 pKa = 11.84GSVRR97 pKa = 11.84RR98 pKa = 11.84TPYY101 pKa = 9.43TKK103 pKa = 10.02PNHH106 pKa = 5.82SQASKK111 pKa = 10.5YY112 pKa = 10.58NSTRR116 pKa = 11.84ITKK119 pKa = 7.54STRR122 pKa = 11.84KK123 pKa = 8.3NTTTAPIITVSPNMTFNPQNSNVSSPRR150 pKa = 11.84VSNALVPYY158 pKa = 9.73NHH160 pKa = 6.32NQAQQGLRR168 pKa = 11.84RR169 pKa = 11.84MVEE172 pKa = 4.2KK173 pKa = 10.56EE174 pKa = 3.77SSAPGLASRR183 pKa = 11.84VLRR186 pKa = 11.84RR187 pKa = 11.84MINVALPTLGSAALASMTGIPSFLTNAAVNAVSSAITSDD226 pKa = 3.39EE227 pKa = 4.09DD228 pKa = 3.6VGPVTPTIPIYY239 pKa = 10.76HH240 pKa = 7.49EE241 pKa = 4.56STDD244 pKa = 3.59SAQPALRR251 pKa = 11.84YY252 pKa = 9.72QPIPQYY258 pKa = 11.11SGGPKK263 pKa = 9.72PDD265 pKa = 4.57DD266 pKa = 4.86DD267 pKa = 5.04SPGLLSTAQNLFEE280 pKa = 5.21NMLAYY285 pKa = 10.59VPTLTTLAGAGHH297 pKa = 6.93KK298 pKa = 10.04VYY300 pKa = 10.62QAMRR304 pKa = 11.84GGVAPYY310 pKa = 10.62VSGTHH315 pKa = 6.6TYY317 pKa = 9.2TPQASYY323 pKa = 10.42IQDD326 pKa = 3.33RR327 pKa = 11.84YY328 pKa = 10.3YY329 pKa = 10.92RR330 pKa = 11.84PAPYY334 pKa = 9.62ISSQQYY340 pKa = 9.78RR341 pKa = 11.84GGSGYY346 pKa = 9.96IPSAACRR353 pKa = 11.84VSVHH357 pKa = 5.99NPRR360 pKa = 11.84QYY362 pKa = 11.48ALRR365 pKa = 11.84GTKK368 pKa = 8.13HH369 pKa = 5.6TYY371 pKa = 10.04RR372 pKa = 11.84NRR374 pKa = 11.84AVHH377 pKa = 6.39NSGKK381 pKa = 10.46YY382 pKa = 7.77YY383 pKa = 10.12VRR385 pKa = 11.84KK386 pKa = 8.28PQTPIEE392 pKa = 4.38ALLQQLVTASKK403 pKa = 10.81AQDD406 pKa = 3.36QEE408 pKa = 4.22HH409 pKa = 6.03TLFRR413 pKa = 11.84PKK415 pKa = 10.84GLLXLPAPEE424 pKa = 5.14ASMLNNPSTTFVPSAFSGFRR444 pKa = 11.84PIPPHH449 pKa = 5.2DD450 pKa = 4.02HH451 pKa = 6.78KK452 pKa = 11.27EE453 pKa = 4.06LVLEE457 pKa = 4.54EE458 pKa = 4.37PQPAFPPPPPPPPPPPPPPATMPVHH483 pKa = 4.93QRR485 pKa = 11.84KK486 pKa = 6.95TEE488 pKa = 4.12SVPFLTPPSSPIILGGPNNLLGRR511 pKa = 11.84TQAFLNEE518 pKa = 3.8IQQGKK523 pKa = 9.21KK524 pKa = 9.78LRR526 pKa = 11.84AAQDD530 pKa = 3.0RR531 pKa = 11.84MLAPLPVTKK540 pKa = 10.25HH541 pKa = 5.56GSPVADD547 pKa = 4.17FHH549 pKa = 7.26SQLADD554 pKa = 3.47VLKK557 pKa = 10.04TGPKK561 pKa = 9.14LRR563 pKa = 11.84KK564 pKa = 8.85VQPKK568 pKa = 8.51HH569 pKa = 4.16TVKK572 pKa = 10.81EE573 pKa = 4.15EE574 pKa = 4.05TMPTNPGDD582 pKa = 3.83RR583 pKa = 11.84IFNSLITKK591 pKa = 9.6IRR593 pKa = 11.84TRR595 pKa = 11.84VSPEE599 pKa = 3.48EE600 pKa = 4.16GLDD603 pKa = 3.5EE604 pKa = 5.65DD605 pKa = 4.14SWW607 pKa = 4.45

Molecular weight: 66.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5200

172

1101

520.0

57.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.904 ± 0.398

2.692 ± 0.43

4.423 ± 0.397

5.942 ± 0.594

3.615 ± 0.466

5.692 ± 0.457

3.481 ± 0.221

4.019 ± 0.357

4.654 ± 0.51

8.115 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.312

4.154 ± 0.214

6.827 ± 0.834

4.635 ± 0.318

6.308 ± 0.5

8.154 ± 0.478

7.077 ± 0.535

7.269 ± 0.309

0.75 ± 0.168

2.769 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski