Lactobacillus vaccinostercus DSM 20634

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Paucilactobacillus; Paucilactobacillus vaccinostercus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2A7D5|A0A0R2A7D5_9LACO Mevalonate kinase OS=Lactobacillus vaccinostercus DSM 20634 OX=1423813 GN=FC26_GL002174 PE=4 SV=1
MM1 pKa = 7.54ASALVVYY8 pKa = 8.24ATITGNNEE16 pKa = 3.46DD17 pKa = 3.72VADD20 pKa = 4.59VITEE24 pKa = 3.9ALEE27 pKa = 4.18DD28 pKa = 3.81LGVDD32 pKa = 3.3VDD34 pKa = 3.96EE35 pKa = 4.97TEE37 pKa = 3.91ITQADD42 pKa = 3.56AEE44 pKa = 4.37EE45 pKa = 5.2FEE47 pKa = 5.11DD48 pKa = 5.27ADD50 pKa = 3.54ICVVCPYY57 pKa = 10.11TYY59 pKa = 10.79DD60 pKa = 3.34EE61 pKa = 4.73GALPEE66 pKa = 4.34EE67 pKa = 4.73GMDD70 pKa = 4.13FYY72 pKa = 11.74EE73 pKa = 5.79DD74 pKa = 3.77LQEE77 pKa = 5.29LDD79 pKa = 4.27LSGKK83 pKa = 9.75IYY85 pKa = 10.61GVAGSGDD92 pKa = 3.77TFYY95 pKa = 11.52LDD97 pKa = 4.59DD98 pKa = 4.08YY99 pKa = 10.62CVSVDD104 pKa = 3.22KK105 pKa = 10.68FAAAFEE111 pKa = 4.32KK112 pKa = 10.95AKK114 pKa = 9.33ATKK117 pKa = 10.01GAEE120 pKa = 3.71AVKK123 pKa = 10.6INLEE127 pKa = 4.14PEE129 pKa = 4.04NEE131 pKa = 4.94DD132 pKa = 3.74INTLNAFAKK141 pKa = 10.64SLVEE145 pKa = 4.13KK146 pKa = 11.22VNDD149 pKa = 3.57

Molecular weight: 16.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2A596|A0A0R2A596_9LACO Uncharacterized protein OS=Lactobacillus vaccinostercus DSM 20634 OX=1423813 GN=FC26_GL002472 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84RR3 pKa = 11.84IKK5 pKa = 10.31LWQLNVLLALVLISVAVLGLCTGVKK30 pKa = 9.18WEE32 pKa = 4.55SLTLLWRR39 pKa = 11.84APASLDD45 pKa = 3.34GRR47 pKa = 11.84IMWTIRR53 pKa = 11.84IPRR56 pKa = 11.84ILSVVMVGALLSVSGLLMQSLSRR79 pKa = 11.84NPIADD84 pKa = 3.74PSILGVNAGAMLALIIGSLLGMSLSIVTTIWLSLVGAAVAFTAVLLIAMTRR135 pKa = 11.84QGLDD139 pKa = 2.94VLRR142 pKa = 11.84FILGGTVFSSFISCLAYY159 pKa = 10.3AGSLLTNTTMQFRR172 pKa = 11.84NLLVGGFSAVTYY184 pKa = 9.2AHH186 pKa = 6.31VRR188 pKa = 11.84VLCGGLIIVLATILLMRR205 pKa = 11.84KK206 pKa = 8.51QLSLLVLDD214 pKa = 4.52DD215 pKa = 3.48TTARR219 pKa = 11.84SLGAKK224 pKa = 9.66PGLTRR229 pKa = 11.84VVAALLVVISAGISVAVAGNIGFVGLGIPQMINYY263 pKa = 6.97VHH265 pKa = 7.76PDD267 pKa = 2.96RR268 pKa = 11.84LEE270 pKa = 4.4RR271 pKa = 11.84NVVPTVLLGAIFMMVVDD288 pKa = 5.24IVAKK292 pKa = 9.57TLTAPSEE299 pKa = 4.3LPLSALSAICGGIFLLLVIMKK320 pKa = 8.86GARR323 pKa = 11.84VTEE326 pKa = 4.23RR327 pKa = 3.33

Molecular weight: 34.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2398

0

2398

735279

50

2674

306.6

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.062

0.543 ± 0.013

5.83 ± 0.054

5.207 ± 0.054

4.214 ± 0.043

6.689 ± 0.045

2.431 ± 0.026

7.076 ± 0.055

5.516 ± 0.049

9.646 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.028

4.529 ± 0.037

3.526 ± 0.034

5.2 ± 0.055

4.155 ± 0.04

5.988 ± 0.111

6.618 ± 0.067

7.249 ± 0.043

1.045 ± 0.021

3.485 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski