Pseudomonas phage vB_PaeM_PA5oct

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5JWD4|A0A4Y5JWD4_9CAUD tRNA(Ile)-2-lysyl-cytidine synthase OS=Pseudomonas phage vB_PaeM_PA5oct OX=2163605 GN=EST35_0433 PE=3 SV=1
MM1 pKa = 7.37YY2 pKa = 9.73DD3 pKa = 3.32KK4 pKa = 11.26NFVSMDD10 pKa = 3.41QMPVIVSDD18 pKa = 4.1LVSEE22 pKa = 4.03IDD24 pKa = 3.48EE25 pKa = 4.8AGISPKK31 pKa = 10.68VIIAISKK38 pKa = 10.28GGLIPATMISYY49 pKa = 10.67YY50 pKa = 9.52YY51 pKa = 10.03DD52 pKa = 4.03CPIVTYY58 pKa = 10.68DD59 pKa = 3.69PVEE62 pKa = 4.53DD63 pKa = 4.45CFSTNNDD70 pKa = 2.96ISSEE74 pKa = 4.22CIIVDD79 pKa = 4.37DD80 pKa = 5.92VIDD83 pKa = 3.56TGNTVNSVISLLEE96 pKa = 4.07DD97 pKa = 3.04EE98 pKa = 5.6FFFTKK103 pKa = 10.52EE104 pKa = 4.33DD105 pKa = 3.12ISKK108 pKa = 10.13IRR110 pKa = 11.84VASIFATRR118 pKa = 11.84NNTIVDD124 pKa = 5.05FYY126 pKa = 10.9HH127 pKa = 7.8DD128 pKa = 4.81EE129 pKa = 4.02ISYY132 pKa = 10.36HH133 pKa = 6.76DD134 pKa = 3.79SVSFEE139 pKa = 3.95FKK141 pKa = 10.83QFII144 pKa = 3.86

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5JU37|A0A4Y5JU37_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_PA5oct OX=2163605 GN=EST35_0327 PE=4 SV=1
MM1 pKa = 7.3NLVHH5 pKa = 6.78LWINGRR11 pKa = 11.84VVTARR16 pKa = 11.84KK17 pKa = 9.32VVSKK21 pKa = 9.14HH22 pKa = 4.21TVRR25 pKa = 11.84NPNSPIDD32 pKa = 3.54IEE34 pKa = 4.5TIDD37 pKa = 3.79VVRR40 pKa = 11.84LRR42 pKa = 11.84LPRR45 pKa = 11.84GVRR48 pKa = 11.84ASSWALRR55 pKa = 11.84KK56 pKa = 9.27AVYY59 pKa = 10.51QLMEE63 pKa = 4.12QSCSCSRR70 pKa = 11.84DD71 pKa = 3.55CCGHH75 pKa = 6.02WFGGARR81 pKa = 11.84KK82 pKa = 9.49IYY84 pKa = 10.36INGRR88 pKa = 11.84DD89 pKa = 3.34ISVVAGYY96 pKa = 9.42QRR98 pKa = 11.84NYY100 pKa = 10.37

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

445

0

445

85498

35

3857

192.1

21.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.418 ± 0.158

1.27 ± 0.077

5.925 ± 0.118

5.766 ± 0.195

4.27 ± 0.097

5.346 ± 0.15

1.861 ± 0.084

8.501 ± 0.137

7.163 ± 0.254

8.141 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.091

7.05 ± 0.151

3.236 ± 0.11

2.991 ± 0.077

3.74 ± 0.097

7.762 ± 0.147

6.688 ± 0.201

6.422 ± 0.117

1.18 ± 0.058

5.059 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski