Amino acid dipepetide frequency for Pseudomonas phage vB_PaeM_PA5oct

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.836AlaAla: 3.836 ± 0.273
0.538AlaCys: 0.538 ± 0.085
3.193AlaAsp: 3.193 ± 0.236
3.439AlaGlu: 3.439 ± 0.269
1.977AlaPhe: 1.977 ± 0.186
3.731AlaGly: 3.731 ± 0.285
0.936AlaHis: 0.936 ± 0.11
4.164AlaIle: 4.164 ± 0.266
3.953AlaLys: 3.953 ± 0.249
4.269AlaLeu: 4.269 ± 0.27
1.252AlaMet: 1.252 ± 0.13
3.287AlaAsn: 3.287 ± 0.204
1.684AlaPro: 1.684 ± 0.163
1.708AlaGln: 1.708 ± 0.16
2.421AlaArg: 2.421 ± 0.192
4.047AlaSer: 4.047 ± 0.259
3.497AlaThr: 3.497 ± 0.314
3.1AlaVal: 3.1 ± 0.254
0.772AlaTrp: 0.772 ± 0.114
2.374AlaTyr: 2.374 ± 0.155
0.0AlaXaa: 0.0 ± 0.0
Cys
0.585CysAla: 0.585 ± 0.093
0.117CysCys: 0.117 ± 0.035
0.503CysAsp: 0.503 ± 0.073
0.632CysGlu: 0.632 ± 0.089
0.456CysPhe: 0.456 ± 0.087
0.655CysGly: 0.655 ± 0.1
0.175CysHis: 0.175 ± 0.051
1.474CysIle: 1.474 ± 0.165
1.17CysLys: 1.17 ± 0.137
0.772CysLeu: 0.772 ± 0.104
0.398CysMet: 0.398 ± 0.077
1.018CysAsn: 1.018 ± 0.142
0.374CysPro: 0.374 ± 0.069
0.304CysGln: 0.304 ± 0.072
0.526CysArg: 0.526 ± 0.084
0.994CysSer: 0.994 ± 0.107
0.83CysThr: 0.83 ± 0.106
0.924CysVal: 0.924 ± 0.104
0.129CysTrp: 0.129 ± 0.038
0.667CysTyr: 0.667 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
3.392AspAla: 3.392 ± 0.23
0.667AspCys: 0.667 ± 0.102
3.24AspAsp: 3.24 ± 0.253
3.626AspGlu: 3.626 ± 0.252
2.877AspPhe: 2.877 ± 0.187
3.228AspGly: 3.228 ± 0.255
0.702AspHis: 0.702 ± 0.101
6.105AspIle: 6.105 ± 0.297
3.755AspLys: 3.755 ± 0.267
4.667AspLeu: 4.667 ± 0.375
1.439AspMet: 1.439 ± 0.111
4.246AspAsn: 4.246 ± 0.211
2.211AspPro: 2.211 ± 0.171
1.404AspGln: 1.404 ± 0.114
1.883AspArg: 1.883 ± 0.16
3.942AspSer: 3.942 ± 0.247
4.386AspThr: 4.386 ± 0.306
4.047AspVal: 4.047 ± 0.282
0.784AspTrp: 0.784 ± 0.11
2.655AspTyr: 2.655 ± 0.179
0.0AspXaa: 0.0 ± 0.0
Glu
2.667GluAla: 2.667 ± 0.264
0.69GluCys: 0.69 ± 0.106
3.216GluAsp: 3.216 ± 0.246
3.918GluGlu: 3.918 ± 0.314
3.088GluPhe: 3.088 ± 0.181
2.351GluGly: 2.351 ± 0.158
1.509GluHis: 1.509 ± 0.18
5.228GluIle: 5.228 ± 0.255
3.509GluLys: 3.509 ± 0.297
6.281GluLeu: 6.281 ± 0.327
1.532GluMet: 1.532 ± 0.134
3.298GluAsn: 3.298 ± 0.201
1.287GluPro: 1.287 ± 0.134
2.269GluGln: 2.269 ± 0.202
2.012GluArg: 2.012 ± 0.171
3.743GluSer: 3.743 ± 0.246
3.415GluThr: 3.415 ± 0.226
3.45GluVal: 3.45 ± 0.19
0.842GluTrp: 0.842 ± 0.108
3.357GluTyr: 3.357 ± 0.203
0.0GluXaa: 0.0 ± 0.0
Phe
2.105PheAla: 2.105 ± 0.148
0.632PheCys: 0.632 ± 0.096
2.866PheAsp: 2.866 ± 0.156
2.409PheGlu: 2.409 ± 0.198
1.462PhePhe: 1.462 ± 0.145
2.269PheGly: 2.269 ± 0.203
0.725PheHis: 0.725 ± 0.099
4.117PheIle: 4.117 ± 0.243
3.17PheLys: 3.17 ± 0.224
3.497PheLeu: 3.497 ± 0.243
0.842PheMet: 0.842 ± 0.108
3.006PheAsn: 3.006 ± 0.228
1.24PhePro: 1.24 ± 0.126
0.854PheGln: 0.854 ± 0.095
1.591PheArg: 1.591 ± 0.15
3.521PheSer: 3.521 ± 0.194
2.597PheThr: 2.597 ± 0.239
2.983PheVal: 2.983 ± 0.191
0.409PheTrp: 0.409 ± 0.071
2.409PheTyr: 2.409 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
2.749GlyAla: 2.749 ± 0.209
0.632GlyCys: 0.632 ± 0.094
2.678GlyAsp: 2.678 ± 0.27
2.526GlyGlu: 2.526 ± 0.161
2.246GlyPhe: 2.246 ± 0.193
2.69GlyGly: 2.69 ± 0.267
1.053GlyHis: 1.053 ± 0.132
4.585GlyIle: 4.585 ± 0.25
4.14GlyLys: 4.14 ± 0.235
4.094GlyLeu: 4.094 ± 0.297
1.333GlyMet: 1.333 ± 0.141
3.953GlyAsn: 3.953 ± 0.242
1.275GlyPro: 1.275 ± 0.146
2.07GlyGln: 2.07 ± 0.214
2.187GlyArg: 2.187 ± 0.162
4.62GlySer: 4.62 ± 0.323
4.222GlyThr: 4.222 ± 0.311
3.252GlyVal: 3.252 ± 0.198
0.573GlyTrp: 0.573 ± 0.079
2.585GlyTyr: 2.585 ± 0.214
0.0GlyXaa: 0.0 ± 0.0
His
1.193HisAla: 1.193 ± 0.129
0.363HisCys: 0.363 ± 0.074
0.912HisAsp: 0.912 ± 0.11
0.749HisGlu: 0.749 ± 0.102
0.889HisPhe: 0.889 ± 0.121
0.924HisGly: 0.924 ± 0.114
0.398HisHis: 0.398 ± 0.082
1.836HisIle: 1.836 ± 0.177
1.298HisLys: 1.298 ± 0.144
1.754HisLeu: 1.754 ± 0.173
0.363HisMet: 0.363 ± 0.07
1.392HisAsn: 1.392 ± 0.139
0.62HisPro: 0.62 ± 0.1
0.48HisGln: 0.48 ± 0.066
0.713HisArg: 0.713 ± 0.091
1.146HisSer: 1.146 ± 0.14
1.263HisThr: 1.263 ± 0.131
1.158HisVal: 1.158 ± 0.147
0.164HisTrp: 0.164 ± 0.046
0.994HisTyr: 0.994 ± 0.097
0.0HisXaa: 0.0 ± 0.0
Ile
4.328IleAla: 4.328 ± 0.196
1.111IleCys: 1.111 ± 0.142
5.369IleAsp: 5.369 ± 0.307
4.994IleGlu: 4.994 ± 0.26
3.579IlePhe: 3.579 ± 0.216
3.895IleGly: 3.895 ± 0.236
1.673IleHis: 1.673 ± 0.156
6.913IleIle: 6.913 ± 0.337
6.527IleLys: 6.527 ± 0.375
7.076IleLeu: 7.076 ± 0.308
1.836IleMet: 1.836 ± 0.156
5.883IleAsn: 5.883 ± 0.289
3.181IlePro: 3.181 ± 0.241
2.62IleGln: 2.62 ± 0.181
3.369IleArg: 3.369 ± 0.206
7.322IleSer: 7.322 ± 0.274
5.415IleThr: 5.415 ± 0.265
5.415IleVal: 5.415 ± 0.285
0.76IleTrp: 0.76 ± 0.091
3.743IleTyr: 3.743 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
2.912LysAla: 2.912 ± 0.275
1.064LysCys: 1.064 ± 0.155
4.234LysAsp: 4.234 ± 0.264
5.076LysGlu: 5.076 ± 0.347
2.947LysPhe: 2.947 ± 0.201
3.123LysGly: 3.123 ± 0.201
1.731LysHis: 1.731 ± 0.168
6.059LysIle: 6.059 ± 0.281
5.205LysLys: 5.205 ± 0.477
6.62LysLeu: 6.62 ± 0.316
1.953LysMet: 1.953 ± 0.179
4.421LysAsn: 4.421 ± 0.268
2.257LysPro: 2.257 ± 0.232
3.17LysGln: 3.17 ± 0.193
2.994LysArg: 2.994 ± 0.285
4.831LysSer: 4.831 ± 0.277
4.374LysThr: 4.374 ± 0.242
3.755LysVal: 3.755 ± 0.223
0.947LysTrp: 0.947 ± 0.125
3.953LysTyr: 3.953 ± 0.245
0.0LysXaa: 0.0 ± 0.0
Leu
4.538LeuAla: 4.538 ± 0.298
1.263LeuCys: 1.263 ± 0.134
5.275LeuAsp: 5.275 ± 0.261
5.181LeuGlu: 5.181 ± 0.319
3.193LeuPhe: 3.193 ± 0.216
4.316LeuGly: 4.316 ± 0.227
1.579LeuHis: 1.579 ± 0.174
5.638LeuIle: 5.638 ± 0.261
6.117LeuLys: 6.117 ± 0.365
6.141LeuLeu: 6.141 ± 0.299
1.708LeuMet: 1.708 ± 0.152
5.603LeuAsn: 5.603 ± 0.279
3.076LeuPro: 3.076 ± 0.185
2.76LeuGln: 2.76 ± 0.186
2.994LeuArg: 2.994 ± 0.175
7.006LeuSer: 7.006 ± 0.404
4.983LeuThr: 4.983 ± 0.287
5.205LeuVal: 5.205 ± 0.254
0.842LeuTrp: 0.842 ± 0.107
3.988LeuTyr: 3.988 ± 0.236
0.0LeuXaa: 0.0 ± 0.0
Met
1.287MetAla: 1.287 ± 0.104
0.234MetCys: 0.234 ± 0.047
1.216MetAsp: 1.216 ± 0.119
0.877MetGlu: 0.877 ± 0.111
1.064MetPhe: 1.064 ± 0.107
1.006MetGly: 1.006 ± 0.113
0.585MetHis: 0.585 ± 0.091
1.45MetIle: 1.45 ± 0.123
1.754MetLys: 1.754 ± 0.153
2.082MetLeu: 2.082 ± 0.176
0.269MetMet: 0.269 ± 0.062
1.614MetAsn: 1.614 ± 0.132
0.924MetPro: 0.924 ± 0.093
0.842MetGln: 0.842 ± 0.102
0.994MetArg: 0.994 ± 0.112
2.0MetSer: 2.0 ± 0.18
1.287MetThr: 1.287 ± 0.123
1.029MetVal: 1.029 ± 0.107
0.269MetTrp: 0.269 ± 0.058
1.322MetTyr: 1.322 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.474AsnAla: 3.474 ± 0.256
0.924AsnCys: 0.924 ± 0.131
3.719AsnAsp: 3.719 ± 0.247
3.895AsnGlu: 3.895 ± 0.214
2.445AsnPhe: 2.445 ± 0.165
4.445AsnGly: 4.445 ± 0.325
0.877AsnHis: 0.877 ± 0.116
6.877AsnIle: 6.877 ± 0.384
6.012AsnLys: 6.012 ± 0.351
4.784AsnLeu: 4.784 ± 0.215
1.38AsnMet: 1.38 ± 0.139
5.649AsnAsn: 5.649 ± 0.345
2.597AsnPro: 2.597 ± 0.234
1.474AsnGln: 1.474 ± 0.142
2.714AsnArg: 2.714 ± 0.147
5.345AsnSer: 5.345 ± 0.259
5.696AsnThr: 5.696 ± 0.303
4.246AsnVal: 4.246 ± 0.237
0.854AsnTrp: 0.854 ± 0.112
3.1AsnTyr: 3.1 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
2.234ProAla: 2.234 ± 0.202
0.316ProCys: 0.316 ± 0.065
2.152ProAsp: 2.152 ± 0.149
2.211ProGlu: 2.211 ± 0.156
1.404ProPhe: 1.404 ± 0.122
2.117ProGly: 2.117 ± 0.179
0.503ProHis: 0.503 ± 0.068
2.48ProIle: 2.48 ± 0.223
1.731ProLys: 1.731 ± 0.141
2.129ProLeu: 2.129 ± 0.146
0.503ProMet: 0.503 ± 0.071
2.316ProAsn: 2.316 ± 0.2
0.912ProPro: 0.912 ± 0.127
0.69ProGln: 0.69 ± 0.098
0.936ProArg: 0.936 ± 0.105
2.445ProSer: 2.445 ± 0.2
2.655ProThr: 2.655 ± 0.276
2.784ProVal: 2.784 ± 0.231
0.351ProTrp: 0.351 ± 0.068
1.497ProTyr: 1.497 ± 0.15
0.0ProXaa: 0.0 ± 0.0
Gln
1.731GlnAla: 1.731 ± 0.188
0.339GlnCys: 0.339 ± 0.073
1.345GlnAsp: 1.345 ± 0.133
1.813GlnGlu: 1.813 ± 0.166
1.205GlnPhe: 1.205 ± 0.122
1.368GlnGly: 1.368 ± 0.127
0.713GlnHis: 0.713 ± 0.114
2.234GlnIle: 2.234 ± 0.147
1.813GlnLys: 1.813 ± 0.181
3.1GlnLeu: 3.1 ± 0.171
0.807GlnMet: 0.807 ± 0.112
2.035GlnAsn: 2.035 ± 0.159
1.029GlnPro: 1.029 ± 0.107
1.228GlnGln: 1.228 ± 0.122
1.193GlnArg: 1.193 ± 0.137
2.047GlnSer: 2.047 ± 0.143
1.731GlnThr: 1.731 ± 0.157
1.731GlnVal: 1.731 ± 0.139
0.444GlnTrp: 0.444 ± 0.073
1.988GlnTyr: 1.988 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
2.047ArgAla: 2.047 ± 0.185
0.597ArgCys: 0.597 ± 0.099
1.825ArgAsp: 1.825 ± 0.141
1.918ArgGlu: 1.918 ± 0.201
1.696ArgPhe: 1.696 ± 0.136
1.521ArgGly: 1.521 ± 0.138
0.842ArgHis: 0.842 ± 0.102
3.24ArgIle: 3.24 ± 0.175
2.994ArgLys: 2.994 ± 0.206
3.661ArgLeu: 3.661 ± 0.244
0.912ArgMet: 0.912 ± 0.099
2.526ArgAsn: 2.526 ± 0.206
1.146ArgPro: 1.146 ± 0.113
1.298ArgGln: 1.298 ± 0.129
1.649ArgArg: 1.649 ± 0.146
2.164ArgSer: 2.164 ± 0.174
2.374ArgThr: 2.374 ± 0.153
2.14ArgVal: 2.14 ± 0.192
0.573ArgTrp: 0.573 ± 0.107
2.281ArgTyr: 2.281 ± 0.174
0.0ArgXaa: 0.0 ± 0.0
Ser
4.585SerAla: 4.585 ± 0.264
0.912SerCys: 0.912 ± 0.107
4.597SerAsp: 4.597 ± 0.3
3.766SerGlu: 3.766 ± 0.206
3.509SerPhe: 3.509 ± 0.204
4.538SerGly: 4.538 ± 0.332
1.216SerHis: 1.216 ± 0.135
6.445SerIle: 6.445 ± 0.282
5.813SerLys: 5.813 ± 0.326
6.082SerLeu: 6.082 ± 0.261
1.684SerMet: 1.684 ± 0.177
5.392SerAsn: 5.392 ± 0.244
2.012SerPro: 2.012 ± 0.157
1.708SerGln: 1.708 ± 0.146
2.421SerArg: 2.421 ± 0.153
6.971SerSer: 6.971 ± 0.381
5.088SerThr: 5.088 ± 0.331
5.86SerVal: 5.86 ± 0.297
0.877SerTrp: 0.877 ± 0.091
4.14SerTyr: 4.14 ± 0.249
0.0SerXaa: 0.0 ± 0.0
Thr
3.392ThrAla: 3.392 ± 0.269
0.491ThrCys: 0.491 ± 0.077
4.491ThrAsp: 4.491 ± 0.272
3.871ThrGlu: 3.871 ± 0.189
3.053ThrPhe: 3.053 ± 0.224
4.667ThrGly: 4.667 ± 0.325
1.205ThrHis: 1.205 ± 0.13
5.462ThrIle: 5.462 ± 0.293
4.374ThrLys: 4.374 ± 0.246
5.427ThrLeu: 5.427 ± 0.269
1.228ThrMet: 1.228 ± 0.132
5.123ThrAsn: 5.123 ± 0.382
2.538ThrPro: 2.538 ± 0.248
1.778ThrGln: 1.778 ± 0.129
2.012ThrArg: 2.012 ± 0.152
5.17ThrSer: 5.17 ± 0.332
4.445ThrThr: 4.445 ± 0.327
4.445ThrVal: 4.445 ± 0.311
0.819ThrTrp: 0.819 ± 0.101
2.889ThrTyr: 2.889 ± 0.181
0.0ThrXaa: 0.0 ± 0.0
Val
3.871ValAla: 3.871 ± 0.303
0.877ValCys: 0.877 ± 0.104
4.211ValAsp: 4.211 ± 0.247
3.591ValGlu: 3.591 ± 0.223
3.17ValPhe: 3.17 ± 0.196
3.427ValGly: 3.427 ± 0.191
1.053ValHis: 1.053 ± 0.115
5.041ValIle: 5.041 ± 0.243
4.117ValLys: 4.117 ± 0.232
4.877ValLeu: 4.877 ± 0.241
1.298ValMet: 1.298 ± 0.13
4.094ValAsn: 4.094 ± 0.266
2.281ValPro: 2.281 ± 0.181
1.661ValGln: 1.661 ± 0.138
2.515ValArg: 2.515 ± 0.166
5.673ValSer: 5.673 ± 0.278
4.105ValThr: 4.105 ± 0.24
4.339ValVal: 4.339 ± 0.252
0.69ValTrp: 0.69 ± 0.086
3.333ValTyr: 3.333 ± 0.195
0.0ValXaa: 0.0 ± 0.0
Trp
0.749TrpAla: 0.749 ± 0.084
0.187TrpCys: 0.187 ± 0.046
0.924TrpAsp: 0.924 ± 0.121
0.608TrpGlu: 0.608 ± 0.088
0.433TrpPhe: 0.433 ± 0.081
0.515TrpGly: 0.515 ± 0.091
0.292TrpHis: 0.292 ± 0.053
0.947TrpIle: 0.947 ± 0.106
0.737TrpLys: 0.737 ± 0.079
0.784TrpLeu: 0.784 ± 0.1
0.327TrpMet: 0.327 ± 0.064
1.053TrpAsn: 1.053 ± 0.133
0.292TrpPro: 0.292 ± 0.059
0.386TrpGln: 0.386 ± 0.063
0.374TrpArg: 0.374 ± 0.064
0.643TrpSer: 0.643 ± 0.096
0.678TrpThr: 0.678 ± 0.097
1.006TrpVal: 1.006 ± 0.134
0.187TrpTrp: 0.187 ± 0.047
0.678TrpTyr: 0.678 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.503TyrAla: 2.503 ± 0.18
0.749TyrCys: 0.749 ± 0.1
3.287TyrAsp: 3.287 ± 0.221
2.561TyrGlu: 2.561 ± 0.164
2.012TyrPhe: 2.012 ± 0.15
2.678TyrGly: 2.678 ± 0.195
0.842TyrHis: 0.842 ± 0.11
4.714TyrIle: 4.714 ± 0.266
3.439TyrLys: 3.439 ± 0.236
3.31TyrLeu: 3.31 ± 0.207
1.029TyrMet: 1.029 ± 0.133
4.597TyrAsn: 4.597 ± 0.282
1.427TyrPro: 1.427 ± 0.146
1.205TyrGln: 1.205 ± 0.12
1.906TyrArg: 1.906 ± 0.154
3.988TyrSer: 3.988 ± 0.235
3.836TyrThr: 3.836 ± 0.304
3.357TyrVal: 3.357 ± 0.188
0.515TyrTrp: 0.515 ± 0.078
2.632TyrTyr: 2.632 ± 0.246
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 445 proteins (85498 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski