Streptomyces phage Manuel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Beephvirinae; Manuelvirus; Streptomyces virus Manuel

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PR27|A0A2H4PR27_9CAUD Uncharacterized protein OS=Streptomyces phage Manuel OX=2053812 GN=SEA_MANUEL_81 PE=4 SV=1
MM1 pKa = 7.29YY2 pKa = 10.48SGIAYY7 pKa = 9.69AVTCHH12 pKa = 7.02DD13 pKa = 3.39GRR15 pKa = 11.84GNAVKK20 pKa = 10.64PEE22 pKa = 3.96VAPLDD27 pKa = 5.07DD28 pKa = 5.32LDD30 pKa = 4.32QVYY33 pKa = 10.44LPLSDD38 pKa = 4.76FIEE41 pKa = 4.25WTEE44 pKa = 5.0LDD46 pKa = 5.5DD47 pKa = 6.87LMDD50 pKa = 4.8DD51 pKa = 4.57CLDD54 pKa = 3.45RR55 pKa = 11.84MYY57 pKa = 10.76DD58 pKa = 2.94AHH60 pKa = 6.27YY61 pKa = 10.34AQDD64 pKa = 3.67AEE66 pKa = 4.59EE67 pKa = 5.43PGLTWEE73 pKa = 4.17VRR75 pKa = 11.84AYY77 pKa = 10.83ALLKK81 pKa = 10.77VII83 pKa = 5.13

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PR19|A0A2H4PR19_9CAUD Uncharacterized protein OS=Streptomyces phage Manuel OX=2053812 GN=SEA_MANUEL_69 PE=4 SV=1
MM1 pKa = 7.62ISFDD5 pKa = 4.03EE6 pKa = 4.56TFMSAGDD13 pKa = 3.6PLVRR17 pKa = 11.84SRR19 pKa = 11.84VPGFGSANRR28 pKa = 11.84TPQTSRR34 pKa = 11.84VHH36 pKa = 6.27KK37 pKa = 10.7SRR39 pKa = 11.84AKK41 pKa = 9.26SAKK44 pKa = 8.31PAKK47 pKa = 9.67AAKK50 pKa = 9.73KK51 pKa = 9.78GLARR55 pKa = 11.84SCVSVKK61 pKa = 10.74SIGEE65 pKa = 3.85RR66 pKa = 11.84TFEE69 pKa = 3.97VRR71 pKa = 11.84VKK73 pKa = 9.95GTLHH77 pKa = 5.99ILGFIRR83 pKa = 11.84MSKK86 pKa = 9.84GRR88 pKa = 11.84AGKK91 pKa = 9.79AYY93 pKa = 9.93SYY95 pKa = 11.31KK96 pKa = 10.43LVSEE100 pKa = 4.29QRR102 pKa = 11.84SHH104 pKa = 7.48NGFSSLNAAVSRR116 pKa = 11.84MLEE119 pKa = 3.9KK120 pKa = 10.87CC121 pKa = 3.45

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12993

38

1646

206.2

22.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.767 ± 0.385

0.877 ± 0.187

6.819 ± 0.309

6.049 ± 0.312

3.371 ± 0.175

8.643 ± 0.354

1.647 ± 0.141

4.395 ± 0.21

5.626 ± 0.434

7.135 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.249

3.856 ± 0.191

4.341 ± 0.218

4.664 ± 0.255

5.334 ± 0.239

6.034 ± 0.304

6.38 ± 0.47

6.881 ± 0.337

1.693 ± 0.117

3.71 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski