Amino acid dipepetide frequency for Streptomyces phage Manuel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.392AlaAla: 11.392 ± 1.382
0.847AlaCys: 0.847 ± 0.256
5.696AlaAsp: 5.696 ± 0.674
5.927AlaGlu: 5.927 ± 0.554
2.54AlaPhe: 2.54 ± 0.337
10.006AlaGly: 10.006 ± 0.999
1.616AlaHis: 1.616 ± 0.273
3.31AlaIle: 3.31 ± 0.674
5.003AlaLys: 5.003 ± 0.722
7.851AlaLeu: 7.851 ± 0.88
2.001AlaMet: 2.001 ± 0.392
4.002AlaAsn: 4.002 ± 0.447
3.695AlaPro: 3.695 ± 0.519
4.926AlaGln: 4.926 ± 0.841
4.233AlaArg: 4.233 ± 0.499
5.619AlaSer: 5.619 ± 0.635
6.466AlaThr: 6.466 ± 0.805
7.235AlaVal: 7.235 ± 0.963
1.693AlaTrp: 1.693 ± 0.301
3.618AlaTyr: 3.618 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
0.308CysAla: 0.308 ± 0.162
0.077CysCys: 0.077 ± 0.077
0.539CysAsp: 0.539 ± 0.292
0.462CysGlu: 0.462 ± 0.205
0.154CysPhe: 0.154 ± 0.113
0.693CysGly: 0.693 ± 0.371
0.231CysHis: 0.231 ± 0.14
0.231CysIle: 0.231 ± 0.179
0.616CysLys: 0.616 ± 0.292
0.693CysLeu: 0.693 ± 0.251
0.385CysMet: 0.385 ± 0.196
0.077CysAsn: 0.077 ± 0.077
0.77CysPro: 0.77 ± 0.29
0.539CysGln: 0.539 ± 0.195
0.539CysArg: 0.539 ± 0.215
0.616CysSer: 0.616 ± 0.183
0.385CysThr: 0.385 ± 0.283
1.155CysVal: 1.155 ± 0.397
0.154CysTrp: 0.154 ± 0.12
0.154CysTyr: 0.154 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
6.619AspAla: 6.619 ± 0.84
0.385AspCys: 0.385 ± 0.177
4.387AspAsp: 4.387 ± 0.637
4.926AspGlu: 4.926 ± 0.675
2.771AspPhe: 2.771 ± 0.379
4.849AspGly: 4.849 ± 0.632
1.078AspHis: 1.078 ± 0.29
4.233AspIle: 4.233 ± 0.556
3.079AspLys: 3.079 ± 0.484
4.387AspLeu: 4.387 ± 0.552
2.309AspMet: 2.309 ± 0.489
2.463AspAsn: 2.463 ± 0.557
4.926AspPro: 4.926 ± 0.73
3.156AspGln: 3.156 ± 0.59
3.233AspArg: 3.233 ± 0.414
3.387AspSer: 3.387 ± 0.51
3.156AspThr: 3.156 ± 0.442
4.387AspVal: 4.387 ± 0.643
1.616AspTrp: 1.616 ± 0.374
2.848AspTyr: 2.848 ± 0.443
0.0AspXaa: 0.0 ± 0.0
Glu
5.388GluAla: 5.388 ± 0.613
0.693GluCys: 0.693 ± 0.283
3.618GluAsp: 3.618 ± 0.44
4.079GluGlu: 4.079 ± 0.541
2.617GluPhe: 2.617 ± 0.557
4.695GluGly: 4.695 ± 0.596
0.924GluHis: 0.924 ± 0.387
3.31GluIle: 3.31 ± 0.492
2.694GluLys: 2.694 ± 0.484
6.004GluLeu: 6.004 ± 0.75
1.539GluMet: 1.539 ± 0.309
1.77GluAsn: 1.77 ± 0.384
2.232GluPro: 2.232 ± 0.492
3.002GluGln: 3.002 ± 0.532
3.002GluArg: 3.002 ± 0.467
3.156GluSer: 3.156 ± 0.508
3.387GluThr: 3.387 ± 0.532
4.464GluVal: 4.464 ± 0.706
1.155GluTrp: 1.155 ± 0.282
2.771GluTyr: 2.771 ± 0.453
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.412
0.231PheCys: 0.231 ± 0.138
3.156PheAsp: 3.156 ± 0.486
2.54PheGlu: 2.54 ± 0.387
1.155PhePhe: 1.155 ± 0.27
3.31PheGly: 3.31 ± 0.528
0.77PheHis: 0.77 ± 0.243
0.847PheIle: 0.847 ± 0.22
1.232PheLys: 1.232 ± 0.26
1.539PheLeu: 1.539 ± 0.346
0.847PheMet: 0.847 ± 0.208
1.77PheAsn: 1.77 ± 0.267
1.847PhePro: 1.847 ± 0.441
1.462PheGln: 1.462 ± 0.358
1.77PheArg: 1.77 ± 0.462
2.617PheSer: 2.617 ± 0.424
2.309PheThr: 2.309 ± 0.445
1.693PheVal: 1.693 ± 0.354
0.308PheTrp: 0.308 ± 0.147
1.232PheTyr: 1.232 ± 0.409
0.0PheXaa: 0.0 ± 0.0
Gly
8.082GlyAla: 8.082 ± 0.82
0.847GlyCys: 0.847 ± 0.362
5.542GlyAsp: 5.542 ± 0.674
5.003GlyGlu: 5.003 ± 0.65
3.233GlyPhe: 3.233 ± 0.576
8.698GlyGly: 8.698 ± 1.196
1.155GlyHis: 1.155 ± 0.348
4.233GlyIle: 4.233 ± 0.745
5.465GlyLys: 5.465 ± 0.712
6.312GlyLeu: 6.312 ± 0.664
2.848GlyMet: 2.848 ± 0.474
3.079GlyAsn: 3.079 ± 0.505
2.694GlyPro: 2.694 ± 0.37
4.233GlyGln: 4.233 ± 0.478
4.233GlyArg: 4.233 ± 0.493
5.234GlySer: 5.234 ± 0.59
5.542GlyThr: 5.542 ± 0.736
5.234GlyVal: 5.234 ± 0.609
1.924GlyTrp: 1.924 ± 0.297
2.848GlyTyr: 2.848 ± 0.515
0.0GlyXaa: 0.0 ± 0.0
His
1.232HisAla: 1.232 ± 0.243
0.231HisCys: 0.231 ± 0.146
1.462HisAsp: 1.462 ± 0.488
1.308HisGlu: 1.308 ± 0.327
0.616HisPhe: 0.616 ± 0.175
1.847HisGly: 1.847 ± 0.37
0.462HisHis: 0.462 ± 0.178
1.001HisIle: 1.001 ± 0.232
0.385HisLys: 0.385 ± 0.174
1.232HisLeu: 1.232 ± 0.383
0.385HisMet: 0.385 ± 0.148
0.539HisAsn: 0.539 ± 0.193
0.462HisPro: 0.462 ± 0.199
0.308HisGln: 0.308 ± 0.146
1.001HisArg: 1.001 ± 0.246
1.155HisSer: 1.155 ± 0.323
0.847HisThr: 0.847 ± 0.315
1.078HisVal: 1.078 ± 0.259
0.231HisTrp: 0.231 ± 0.173
0.693HisTyr: 0.693 ± 0.265
0.0HisXaa: 0.0 ± 0.0
Ile
3.31IleAla: 3.31 ± 0.56
0.308IleCys: 0.308 ± 0.143
3.387IleAsp: 3.387 ± 0.448
3.079IleGlu: 3.079 ± 0.479
1.385IlePhe: 1.385 ± 0.33
3.233IleGly: 3.233 ± 0.482
0.847IleHis: 0.847 ± 0.252
2.232IleIle: 2.232 ± 0.479
3.695IleLys: 3.695 ± 0.635
3.541IleLeu: 3.541 ± 0.429
1.385IleMet: 1.385 ± 0.339
1.385IleAsn: 1.385 ± 0.282
1.77IlePro: 1.77 ± 0.412
1.308IleGln: 1.308 ± 0.318
2.694IleArg: 2.694 ± 0.438
2.155IleSer: 2.155 ± 0.379
3.156IleThr: 3.156 ± 0.471
3.079IleVal: 3.079 ± 0.49
0.308IleTrp: 0.308 ± 0.149
1.693IleTyr: 1.693 ± 0.376
0.0IleXaa: 0.0 ± 0.0
Lys
6.081LysAla: 6.081 ± 0.729
0.385LysCys: 0.385 ± 0.184
4.772LysAsp: 4.772 ± 0.885
3.233LysGlu: 3.233 ± 0.535
2.001LysPhe: 2.001 ± 0.334
3.925LysGly: 3.925 ± 0.508
1.385LysHis: 1.385 ± 0.349
1.77LysIle: 1.77 ± 0.329
3.464LysLys: 3.464 ± 0.496
4.541LysLeu: 4.541 ± 0.655
1.385LysMet: 1.385 ± 0.307
2.617LysAsn: 2.617 ± 0.537
2.54LysPro: 2.54 ± 0.44
2.617LysGln: 2.617 ± 0.433
2.771LysArg: 2.771 ± 0.551
2.771LysSer: 2.771 ± 0.476
2.848LysThr: 2.848 ± 0.495
4.464LysVal: 4.464 ± 0.572
1.001LysTrp: 1.001 ± 0.319
1.693LysTyr: 1.693 ± 0.531
0.0LysXaa: 0.0 ± 0.0
Leu
6.85LeuAla: 6.85 ± 0.832
0.385LeuCys: 0.385 ± 0.169
5.234LeuAsp: 5.234 ± 0.711
3.618LeuGlu: 3.618 ± 0.471
2.848LeuPhe: 2.848 ± 0.426
5.465LeuGly: 5.465 ± 0.612
1.078LeuHis: 1.078 ± 0.281
2.848LeuIle: 2.848 ± 0.45
4.387LeuLys: 4.387 ± 0.64
3.772LeuLeu: 3.772 ± 0.471
1.155LeuMet: 1.155 ± 0.28
3.079LeuAsn: 3.079 ± 0.474
2.925LeuPro: 2.925 ± 0.448
3.925LeuGln: 3.925 ± 0.679
4.926LeuArg: 4.926 ± 0.625
5.542LeuSer: 5.542 ± 0.759
4.464LeuThr: 4.464 ± 0.524
5.696LeuVal: 5.696 ± 0.79
1.155LeuTrp: 1.155 ± 0.303
2.001LeuTyr: 2.001 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
4.849MetAla: 4.849 ± 0.617
0.154MetCys: 0.154 ± 0.126
2.078MetAsp: 2.078 ± 0.391
1.462MetGlu: 1.462 ± 0.313
0.616MetPhe: 0.616 ± 0.217
2.617MetGly: 2.617 ± 0.469
0.0MetHis: 0.0 ± 0.0
1.155MetIle: 1.155 ± 0.33
1.462MetLys: 1.462 ± 0.469
1.462MetLeu: 1.462 ± 0.292
0.77MetMet: 0.77 ± 0.265
1.232MetAsn: 1.232 ± 0.276
0.924MetPro: 0.924 ± 0.335
1.078MetGln: 1.078 ± 0.25
1.462MetArg: 1.462 ± 0.365
2.386MetSer: 2.386 ± 0.44
1.539MetThr: 1.539 ± 0.403
1.616MetVal: 1.616 ± 0.266
0.385MetTrp: 0.385 ± 0.125
0.539MetTyr: 0.539 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
3.772AsnAla: 3.772 ± 0.56
0.77AsnCys: 0.77 ± 0.293
2.001AsnAsp: 2.001 ± 0.486
2.232AsnGlu: 2.232 ± 0.403
1.078AsnPhe: 1.078 ± 0.261
3.925AsnGly: 3.925 ± 0.587
0.77AsnHis: 0.77 ± 0.235
1.308AsnIle: 1.308 ± 0.244
2.617AsnLys: 2.617 ± 0.377
3.387AsnLeu: 3.387 ± 0.528
1.001AsnMet: 1.001 ± 0.25
1.539AsnAsn: 1.539 ± 0.394
2.463AsnPro: 2.463 ± 0.325
1.462AsnGln: 1.462 ± 0.333
1.539AsnArg: 1.539 ± 0.371
2.155AsnSer: 2.155 ± 0.451
2.771AsnThr: 2.771 ± 0.368
1.616AsnVal: 1.616 ± 0.293
0.693AsnTrp: 0.693 ± 0.232
1.462AsnTyr: 1.462 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
4.156ProAla: 4.156 ± 0.464
0.385ProCys: 0.385 ± 0.202
3.079ProAsp: 3.079 ± 0.413
2.694ProGlu: 2.694 ± 0.433
1.616ProPhe: 1.616 ± 0.332
4.31ProGly: 4.31 ± 0.774
0.847ProHis: 0.847 ± 0.259
1.462ProIle: 1.462 ± 0.404
2.001ProLys: 2.001 ± 0.362
3.002ProLeu: 3.002 ± 0.373
1.232ProMet: 1.232 ± 0.275
1.847ProAsn: 1.847 ± 0.35
1.308ProPro: 1.308 ± 0.379
1.539ProGln: 1.539 ± 0.387
2.386ProArg: 2.386 ± 0.442
2.232ProSer: 2.232 ± 0.403
3.695ProThr: 3.695 ± 0.55
3.233ProVal: 3.233 ± 0.453
0.693ProTrp: 0.693 ± 0.223
1.693ProTyr: 1.693 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
5.465GlnAla: 5.465 ± 0.737
0.154GlnCys: 0.154 ± 0.113
2.925GlnAsp: 2.925 ± 0.363
2.54GlnGlu: 2.54 ± 0.438
1.232GlnPhe: 1.232 ± 0.311
2.309GlnGly: 2.309 ± 0.462
0.462GlnHis: 0.462 ± 0.161
2.155GlnIle: 2.155 ± 0.44
2.617GlnLys: 2.617 ± 0.494
4.002GlnLeu: 4.002 ± 0.592
1.539GlnMet: 1.539 ± 0.453
1.462GlnAsn: 1.462 ± 0.403
2.155GlnPro: 2.155 ± 0.418
2.232GlnGln: 2.232 ± 0.396
3.618GlnArg: 3.618 ± 0.579
3.002GlnSer: 3.002 ± 0.41
3.156GlnThr: 3.156 ± 0.471
2.694GlnVal: 2.694 ± 0.446
0.77GlnTrp: 0.77 ± 0.255
2.155GlnTyr: 2.155 ± 0.357
0.0GlnXaa: 0.0 ± 0.0
Arg
5.773ArgAla: 5.773 ± 0.64
0.847ArgCys: 0.847 ± 0.315
3.618ArgAsp: 3.618 ± 0.443
3.618ArgGlu: 3.618 ± 0.613
1.616ArgPhe: 1.616 ± 0.398
3.849ArgGly: 3.849 ± 0.482
0.539ArgHis: 0.539 ± 0.176
2.463ArgIle: 2.463 ± 0.429
2.771ArgLys: 2.771 ± 0.477
3.541ArgLeu: 3.541 ± 0.578
1.77ArgMet: 1.77 ± 0.339
2.463ArgAsn: 2.463 ± 0.463
1.847ArgPro: 1.847 ± 0.34
2.386ArgGln: 2.386 ± 0.391
3.002ArgArg: 3.002 ± 0.576
2.694ArgSer: 2.694 ± 0.547
3.464ArgThr: 3.464 ± 0.473
4.156ArgVal: 4.156 ± 0.503
0.77ArgTrp: 0.77 ± 0.245
2.155ArgTyr: 2.155 ± 0.47
0.0ArgXaa: 0.0 ± 0.0
Ser
5.465SerAla: 5.465 ± 0.699
0.462SerCys: 0.462 ± 0.181
3.695SerAsp: 3.695 ± 0.544
3.156SerGlu: 3.156 ± 0.598
1.847SerPhe: 1.847 ± 0.361
6.312SerGly: 6.312 ± 0.909
0.77SerHis: 0.77 ± 0.197
3.002SerIle: 3.002 ± 0.461
2.617SerLys: 2.617 ± 0.586
3.925SerLeu: 3.925 ± 0.455
1.693SerMet: 1.693 ± 0.378
2.617SerAsn: 2.617 ± 0.381
2.463SerPro: 2.463 ± 0.381
2.848SerGln: 2.848 ± 0.508
3.618SerArg: 3.618 ± 0.692
4.233SerSer: 4.233 ± 0.643
4.079SerThr: 4.079 ± 0.626
4.233SerVal: 4.233 ± 0.523
1.232SerTrp: 1.232 ± 0.33
2.001SerTyr: 2.001 ± 0.33
0.0SerXaa: 0.0 ± 0.0
Thr
5.927ThrAla: 5.927 ± 0.852
0.616ThrCys: 0.616 ± 0.281
4.233ThrAsp: 4.233 ± 0.675
3.079ThrGlu: 3.079 ± 0.576
2.617ThrPhe: 2.617 ± 0.425
7.004ThrGly: 7.004 ± 0.959
1.078ThrHis: 1.078 ± 0.346
3.079ThrIle: 3.079 ± 0.493
3.31ThrLys: 3.31 ± 0.636
4.002ThrLeu: 4.002 ± 0.645
1.924ThrMet: 1.924 ± 0.419
1.847ThrAsn: 1.847 ± 0.362
4.156ThrPro: 4.156 ± 0.649
3.541ThrGln: 3.541 ± 0.478
2.232ThrArg: 2.232 ± 0.381
3.233ThrSer: 3.233 ± 0.653
4.079ThrThr: 4.079 ± 0.774
4.618ThrVal: 4.618 ± 0.637
0.924ThrTrp: 0.924 ± 0.223
2.309ThrTyr: 2.309 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
5.234ValAla: 5.234 ± 0.666
0.462ValCys: 0.462 ± 0.18
5.388ValAsp: 5.388 ± 0.521
4.079ValGlu: 4.079 ± 0.675
2.155ValPhe: 2.155 ± 0.371
4.772ValGly: 4.772 ± 0.483
1.847ValHis: 1.847 ± 0.421
2.925ValIle: 2.925 ± 0.459
4.772ValLys: 4.772 ± 0.558
4.849ValLeu: 4.849 ± 0.647
2.309ValMet: 2.309 ± 0.323
2.386ValAsn: 2.386 ± 0.397
2.463ValPro: 2.463 ± 0.56
3.233ValGln: 3.233 ± 0.703
4.541ValArg: 4.541 ± 0.692
5.08ValSer: 5.08 ± 0.62
4.772ValThr: 4.772 ± 0.724
3.849ValVal: 3.849 ± 0.728
1.001ValTrp: 1.001 ± 0.241
2.694ValTyr: 2.694 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
1.539TrpAla: 1.539 ± 0.29
0.154TrpCys: 0.154 ± 0.1
1.232TrpAsp: 1.232 ± 0.308
1.155TrpGlu: 1.155 ± 0.259
0.462TrpPhe: 0.462 ± 0.187
1.078TrpGly: 1.078 ± 0.305
0.0TrpHis: 0.0 ± 0.0
0.77TrpIle: 0.77 ± 0.234
1.385TrpLys: 1.385 ± 0.374
0.693TrpLeu: 0.693 ± 0.224
0.231TrpMet: 0.231 ± 0.186
0.616TrpAsn: 0.616 ± 0.265
0.616TrpPro: 0.616 ± 0.243
0.847TrpGln: 0.847 ± 0.275
0.924TrpArg: 0.924 ± 0.27
0.924TrpSer: 0.924 ± 0.248
1.693TrpThr: 1.693 ± 0.359
1.385TrpVal: 1.385 ± 0.222
0.308TrpTrp: 0.308 ± 0.144
0.924TrpTyr: 0.924 ± 0.303
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.156TyrAla: 3.156 ± 0.729
0.385TyrCys: 0.385 ± 0.177
2.155TyrAsp: 2.155 ± 0.492
2.309TyrGlu: 2.309 ± 0.488
1.155TyrPhe: 1.155 ± 0.288
3.541TyrGly: 3.541 ± 0.469
0.616TyrHis: 0.616 ± 0.229
1.616TyrIle: 1.616 ± 0.355
2.694TyrLys: 2.694 ± 0.4
2.617TyrLeu: 2.617 ± 0.319
1.001TyrMet: 1.001 ± 0.304
1.77TyrAsn: 1.77 ± 0.346
1.155TyrPro: 1.155 ± 0.301
2.001TyrGln: 2.001 ± 0.345
1.616TyrArg: 1.616 ± 0.296
2.155TyrSer: 2.155 ± 0.392
2.001TyrThr: 2.001 ± 0.403
2.925TyrVal: 2.925 ± 0.42
0.616TyrTrp: 0.616 ± 0.205
1.616TyrTyr: 1.616 ± 0.284
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski