Amino acid dipepetide frequency for Pusillimonas sp. (strain T7-7)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.134AlaAla: 14.134 ± 0.159	1.257AlaCys: 1.257 ± 0.032	5.941AlaAsp: 5.941 ± 0.073	5.789AlaGlu: 5.789 ± 0.073	3.753AlaPhe: 3.753 ± 0.056	9.959AlaGly: 9.959 ± 0.139	2.298AlaHis: 2.298 ± 0.048	6.011AlaIle: 6.011 ± 0.08	3.671AlaLys: 3.671 ± 0.073	13.026AlaLeu: 13.026 ± 0.149	3.314AlaMet: 3.314 ± 0.054	3.031AlaAsn: 3.031 ± 0.058	4.697AlaPro: 4.697 ± 0.084	5.499AlaGln: 5.499 ± 0.074	6.998AlaArg: 6.998 ± 0.088	6.872AlaSer: 6.872 ± 0.086	5.428AlaThr: 5.428 ± 0.074	8.026AlaVal: 8.026 ± 0.098	1.69AlaTrp: 1.69 ± 0.047	2.737AlaTyr: 2.737 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.041CysAla: 1.041 ± 0.034	0.142CysCys: 0.142 ± 0.011	0.474CysAsp: 0.474 ± 0.023	0.441CysGlu: 0.441 ± 0.018	0.346CysPhe: 0.346 ± 0.019	0.893CysGly: 0.893 ± 0.031	0.254CysHis: 0.254 ± 0.014	0.46CysIle: 0.46 ± 0.021	0.278CysLys: 0.278 ± 0.015	0.892CysLeu: 0.892 ± 0.03	0.239CysMet: 0.239 ± 0.015	0.277CysAsn: 0.277 ± 0.015	0.462CysPro: 0.462 ± 0.021	0.304CysGln: 0.304 ± 0.017	0.506CysArg: 0.506 ± 0.024	0.543CysSer: 0.543 ± 0.023	0.469CysThr: 0.469 ± 0.022	0.726CysVal: 0.726 ± 0.023	0.127CysTrp: 0.127 ± 0.011	0.214CysTyr: 0.214 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.989AspAla: 5.989 ± 0.081	0.451AspCys: 0.451 ± 0.021	2.908AspAsp: 2.908 ± 0.053	3.163AspGlu: 3.163 ± 0.057	2.051AspPhe: 2.051 ± 0.046	4.333AspGly: 4.333 ± 0.085	1.224AspHis: 1.224 ± 0.032	3.242AspIle: 3.242 ± 0.063	1.87AspLys: 1.87 ± 0.044	5.366AspLeu: 5.366 ± 0.068	1.423AspMet: 1.423 ± 0.032	1.442AspAsn: 1.442 ± 0.039	2.766AspPro: 2.766 ± 0.052	2.084AspGln: 2.084 ± 0.046	2.926AspArg: 2.926 ± 0.046	2.682AspSer: 2.682 ± 0.048	2.909AspThr: 2.909 ± 0.052	3.93AspVal: 3.93 ± 0.07	0.895AspTrp: 0.895 ± 0.027	1.619AspTyr: 1.619 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.864GluAla: 5.864 ± 0.09	0.404GluCys: 0.404 ± 0.023	2.197GluAsp: 2.197 ± 0.046	2.535GluGlu: 2.535 ± 0.053	1.818GluPhe: 1.818 ± 0.047	3.55GluGly: 3.55 ± 0.059	1.495GluHis: 1.495 ± 0.042	2.863GluIle: 2.863 ± 0.054	2.045GluLys: 2.045 ± 0.051	5.89GluLeu: 5.89 ± 0.09	1.263GluMet: 1.263 ± 0.034	1.626GluAsn: 1.626 ± 0.035	2.476GluPro: 2.476 ± 0.053	3.148GluGln: 3.148 ± 0.068	4.088GluArg: 4.088 ± 0.07	2.906GluSer: 2.906 ± 0.05	2.67GluThr: 2.67 ± 0.05	3.665GluVal: 3.665 ± 0.061	0.706GluTrp: 0.706 ± 0.022	1.252GluTyr: 1.252 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.667PheAla: 3.667 ± 0.054	0.383PheCys: 0.383 ± 0.017	2.419PheAsp: 2.419 ± 0.047	1.947PheGlu: 1.947 ± 0.041	1.391PhePhe: 1.391 ± 0.044	3.21PheGly: 3.21 ± 0.059	0.732PheHis: 0.732 ± 0.029	1.851PheIle: 1.851 ± 0.041	1.244PheLys: 1.244 ± 0.038	3.242PheLeu: 3.242 ± 0.057	0.945PheMet: 0.945 ± 0.028	1.27PheAsn: 1.27 ± 0.034	1.5PhePro: 1.5 ± 0.038	1.191PheGln: 1.191 ± 0.032	1.744PheArg: 1.744 ± 0.045	2.548PheSer: 2.548 ± 0.052	1.903PheThr: 1.903 ± 0.038	2.643PheVal: 2.643 ± 0.055	0.534PheTrp: 0.534 ± 0.023	0.95PheTyr: 0.95 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	8.319GlyAla: 8.319 ± 0.122	0.802GlyCys: 0.802 ± 0.028	3.836GlyAsp: 3.836 ± 0.106	3.929GlyGlu: 3.929 ± 0.07	3.128GlyPhe: 3.128 ± 0.061	6.667GlyGly: 6.667 ± 0.226	1.922GlyHis: 1.922 ± 0.039	4.631GlyIle: 4.631 ± 0.078	3.587GlyLys: 3.587 ± 0.064	9.041GlyLeu: 9.041 ± 0.1	2.476GlyMet: 2.476 ± 0.046	2.432GlyAsn: 2.432 ± 0.069	2.928GlyPro: 2.928 ± 0.05	3.62GlyGln: 3.62 ± 0.063	4.918GlyArg: 4.918 ± 0.069	5.019GlySer: 5.019 ± 0.1	4.368GlyThr: 4.368 ± 0.095	6.225GlyVal: 6.225 ± 0.077	1.304GlyTrp: 1.304 ± 0.036	2.48GlyTyr: 2.48 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.623HisAla: 2.623 ± 0.055	0.26HisCys: 0.26 ± 0.016	1.248HisAsp: 1.248 ± 0.035	1.274HisGlu: 1.274 ± 0.034	0.846HisPhe: 0.846 ± 0.027	1.932HisGly: 1.932 ± 0.038	0.621HisHis: 0.621 ± 0.028	1.299HisIle: 1.299 ± 0.033	0.676HisLys: 0.676 ± 0.025	2.166HisLeu: 2.166 ± 0.049	0.561HisMet: 0.561 ± 0.023	0.659HisAsn: 0.659 ± 0.027	1.363HisPro: 1.363 ± 0.036	0.872HisGln: 0.872 ± 0.033	1.248HisArg: 1.248 ± 0.038	1.167HisSer: 1.167 ± 0.031	1.215HisThr: 1.215 ± 0.03	1.544HisVal: 1.544 ± 0.038	0.346HisTrp: 0.346 ± 0.016	0.67HisTyr: 0.67 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.411IleAla: 6.411 ± 0.082	0.457IleCys: 0.457 ± 0.022	3.292IleAsp: 3.292 ± 0.063	3.489IleGlu: 3.489 ± 0.059	1.69IlePhe: 1.69 ± 0.042	4.512IleGly: 4.512 ± 0.064	1.061IleHis: 1.061 ± 0.034	2.621IleIle: 2.621 ± 0.053	2.055IleLys: 2.055 ± 0.043	4.539IleLeu: 4.539 ± 0.07	1.244IleMet: 1.244 ± 0.034	1.872IleAsn: 1.872 ± 0.046	2.63IlePro: 2.63 ± 0.052	1.889IleGln: 1.889 ± 0.04	2.884IleArg: 2.884 ± 0.052	3.446IleSer: 3.446 ± 0.056	3.076IleThr: 3.076 ± 0.052	4.114IleVal: 4.114 ± 0.064	0.62IleTrp: 0.62 ± 0.025	1.206IleTyr: 1.206 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.996LysAla: 3.996 ± 0.083	0.164LysCys: 0.164 ± 0.011	1.725LysAsp: 1.725 ± 0.042	1.837LysGlu: 1.837 ± 0.047	0.954LysPhe: 0.954 ± 0.033	2.56LysGly: 2.56 ± 0.051	0.813LysHis: 0.813 ± 0.029	1.783LysIle: 1.783 ± 0.049	1.616LysLys: 1.616 ± 0.055	3.695LysLeu: 3.695 ± 0.066	0.936LysMet: 0.936 ± 0.026	1.178LysAsn: 1.178 ± 0.034	2.076LysPro: 2.076 ± 0.055	1.717LysGln: 1.717 ± 0.04	2.27LysArg: 2.27 ± 0.05	1.985LysSer: 1.985 ± 0.042	2.129LysThr: 2.129 ± 0.051	2.615LysVal: 2.615 ± 0.052	0.436LysTrp: 0.436 ± 0.02	0.733LysTyr: 0.733 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.663LeuAla: 13.663 ± 0.141	1.065LeuCys: 1.065 ± 0.029	6.135LeuAsp: 6.135 ± 0.079	5.393LeuGlu: 5.393 ± 0.083	3.734LeuPhe: 3.734 ± 0.066	8.574LeuGly: 8.574 ± 0.094	2.301LeuHis: 2.301 ± 0.051	5.512LeuIle: 5.512 ± 0.076	3.719LeuLys: 3.719 ± 0.064	11.072LeuLeu: 11.072 ± 0.137	2.637LeuMet: 2.637 ± 0.056	3.36LeuAsn: 3.36 ± 0.061	5.717LeuPro: 5.717 ± 0.083	4.138LeuGln: 4.138 ± 0.061	6.694LeuArg: 6.694 ± 0.093	6.96LeuSer: 6.96 ± 0.079	5.718LeuThr: 5.718 ± 0.076	7.175LeuVal: 7.175 ± 0.089	1.198LeuTrp: 1.198 ± 0.031	2.3LeuTyr: 2.3 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.132MetAla: 3.132 ± 0.059	0.174MetCys: 0.174 ± 0.011	1.166MetAsp: 1.166 ± 0.033	1.096MetGlu: 1.096 ± 0.032	0.81MetPhe: 0.81 ± 0.026	2.021MetGly: 2.021 ± 0.044	0.578MetHis: 0.578 ± 0.021	1.238MetIle: 1.238 ± 0.031	1.042MetLys: 1.042 ± 0.025	3.049MetLeu: 3.049 ± 0.06	0.727MetMet: 0.727 ± 0.026	0.967MetAsn: 0.967 ± 0.028	1.504MetPro: 1.504 ± 0.033	1.273MetGln: 1.273 ± 0.034	1.648MetArg: 1.648 ± 0.042	1.871MetSer: 1.871 ± 0.04	1.602MetThr: 1.602 ± 0.035	1.894MetVal: 1.894 ± 0.044	0.241MetTrp: 0.241 ± 0.015	0.467MetTyr: 0.467 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.312AsnAla: 3.312 ± 0.059	0.251AsnCys: 0.251 ± 0.012	1.64AsnAsp: 1.64 ± 0.054	1.581AsnGlu: 1.581 ± 0.034	1.091AsnPhe: 1.091 ± 0.033	2.494AsnGly: 2.494 ± 0.057	0.616AsnHis: 0.616 ± 0.02	1.788AsnIle: 1.788 ± 0.038	1.084AsnLys: 1.084 ± 0.033	2.994AsnLeu: 2.994 ± 0.055	0.809AsnMet: 0.809 ± 0.026	1.022AsnAsn: 1.022 ± 0.035	1.958AsnPro: 1.958 ± 0.044	1.169AsnGln: 1.169 ± 0.031	1.809AsnArg: 1.809 ± 0.036	1.623AsnSer: 1.623 ± 0.041	1.909AsnThr: 1.909 ± 0.048	2.135AsnVal: 2.135 ± 0.051	0.497AsnTrp: 0.497 ± 0.021	0.784AsnTyr: 0.784 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.635ProAla: 5.635 ± 0.074	0.353ProCys: 0.353 ± 0.017	3.42ProAsp: 3.42 ± 0.055	3.395ProGlu: 3.395 ± 0.059	1.706ProPhe: 1.706 ± 0.037	4.2ProGly: 4.2 ± 0.071	0.967ProHis: 0.967 ± 0.026	2.276ProIle: 2.276 ± 0.046	1.579ProLys: 1.579 ± 0.036	4.729ProLeu: 4.729 ± 0.076	1.246ProMet: 1.246 ± 0.032	1.425ProAsn: 1.425 ± 0.029	2.096ProPro: 2.096 ± 0.053	1.942ProGln: 1.942 ± 0.046	2.308ProArg: 2.308 ± 0.048	2.848ProSer: 2.848 ± 0.054	2.367ProThr: 2.367 ± 0.044	4.106ProVal: 4.106 ± 0.061	0.683ProTrp: 0.683 ± 0.025	1.279ProTyr: 1.279 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	6.386GlnAla: 6.386 ± 0.084	0.325GlnCys: 0.325 ± 0.017	2.064GlnAsp: 2.064 ± 0.042	2.181GlnGlu: 2.181 ± 0.051	1.291GlnPhe: 1.291 ± 0.029	3.508GlnGly: 3.508 ± 0.068	1.097GlnHis: 1.097 ± 0.035	2.199GlnIle: 2.199 ± 0.044	1.374GlnLys: 1.374 ± 0.039	4.337GlnLeu: 4.337 ± 0.075	1.046GlnMet: 1.046 ± 0.026	1.194GlnAsn: 1.194 ± 0.035	1.996GlnPro: 1.996 ± 0.044	2.249GlnGln: 2.249 ± 0.049	2.925GlnArg: 2.925 ± 0.054	2.292GlnSer: 2.292 ± 0.046	2.28GlnThr: 2.28 ± 0.047	3.104GlnVal: 3.104 ± 0.05	0.687GlnTrp: 0.687 ± 0.027	0.988GlnTyr: 0.988 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	5.877ArgAla: 5.877 ± 0.076	0.5ArgCys: 0.5 ± 0.022	2.988ArgAsp: 2.988 ± 0.048	3.379ArgGlu: 3.379 ± 0.06	2.295ArgPhe: 2.295 ± 0.043	3.92ArgGly: 3.92 ± 0.062	1.703ArgHis: 1.703 ± 0.044	3.561ArgIle: 3.561 ± 0.055	2.316ArgLys: 2.316 ± 0.05	7.111ArgLeu: 7.111 ± 0.092	1.69ArgMet: 1.69 ± 0.044	2.054ArgAsn: 2.054 ± 0.041	2.756ArgPro: 2.756 ± 0.056	3.008ArgGln: 3.008 ± 0.06	4.122ArgArg: 4.122 ± 0.076	3.557ArgSer: 3.557 ± 0.054	2.835ArgThr: 2.835 ± 0.055	4.284ArgVal: 4.284 ± 0.068	0.961ArgTrp: 0.961 ± 0.03	2.002ArgTyr: 2.002 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.264SerAla: 6.264 ± 0.085	0.526SerCys: 0.526 ± 0.021	3.052SerAsp: 3.052 ± 0.051	2.752SerGlu: 2.752 ± 0.046	2.286SerPhe: 2.286 ± 0.044	5.797SerGly: 5.797 ± 0.097	1.273SerHis: 1.273 ± 0.04	3.35SerIle: 3.35 ± 0.052	1.938SerLys: 1.938 ± 0.046	6.519SerLeu: 6.519 ± 0.079	1.726SerMet: 1.726 ± 0.039	1.814SerAsn: 1.814 ± 0.041	2.769SerPro: 2.769 ± 0.053	2.276SerGln: 2.276 ± 0.048	3.595SerArg: 3.595 ± 0.057	3.899SerSer: 3.899 ± 0.069	3.388SerThr: 3.388 ± 0.064	4.445SerVal: 4.445 ± 0.071	0.912SerTrp: 0.912 ± 0.03	1.578SerTyr: 1.578 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.562ThrAla: 5.562 ± 0.084	0.422ThrCys: 0.422 ± 0.019	2.763ThrAsp: 2.763 ± 0.052	2.639ThrGlu: 2.639 ± 0.046	1.664ThrPhe: 1.664 ± 0.038	4.835ThrGly: 4.835 ± 0.075	1.257ThrHis: 1.257 ± 0.032	2.752ThrIle: 2.752 ± 0.05	1.304ThrLys: 1.304 ± 0.041	6.464ThrLeu: 6.464 ± 0.083	1.247ThrMet: 1.247 ± 0.036	1.382ThrAsn: 1.382 ± 0.039	3.477ThrPro: 3.477 ± 0.06	2.243ThrGln: 2.243 ± 0.042	3.124ThrArg: 3.124 ± 0.054	2.925ThrSer: 2.925 ± 0.057	2.886ThrThr: 2.886 ± 0.058	4.295ThrVal: 4.295 ± 0.07	0.633ThrTrp: 0.633 ± 0.023	1.175ThrTyr: 1.175 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.275ValAla: 8.275 ± 0.093	0.746ValCys: 0.746 ± 0.027	3.951ValAsp: 3.951 ± 0.068	3.754ValGlu: 3.754 ± 0.064	2.9ValPhe: 2.9 ± 0.046	5.426ValGly: 5.426 ± 0.077	1.524ValHis: 1.524 ± 0.033	3.849ValIle: 3.849 ± 0.072	2.504ValLys: 2.504 ± 0.049	8.323ValLeu: 8.323 ± 0.09	1.985ValMet: 1.985 ± 0.047	2.403ValAsn: 2.403 ± 0.049	3.512ValPro: 3.512 ± 0.057	3.087ValGln: 3.087 ± 0.051	4.303ValArg: 4.303 ± 0.064	4.735ValSer: 4.735 ± 0.075	3.784ValThr: 3.784 ± 0.066	5.989ValVal: 5.989 ± 0.089	0.91ValTrp: 0.91 ± 0.024	1.696ValTyr: 1.696 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.268TrpAla: 1.268 ± 0.031	0.137TrpCys: 0.137 ± 0.012	0.577TrpAsp: 0.577 ± 0.022	0.555TrpGlu: 0.555 ± 0.022	0.583TrpPhe: 0.583 ± 0.023	0.875TrpGly: 0.875 ± 0.029	0.372TrpHis: 0.372 ± 0.02	0.699TrpIle: 0.699 ± 0.023	0.474TrpLys: 0.474 ± 0.021	2.061TrpLeu: 2.061 ± 0.051	0.403TrpMet: 0.403 ± 0.017	0.497TrpAsn: 0.497 ± 0.023	0.654TrpPro: 0.654 ± 0.025	0.759TrpGln: 0.759 ± 0.028	1.103TrpArg: 1.103 ± 0.036	0.806TrpSer: 0.806 ± 0.026	0.665TrpThr: 0.665 ± 0.026	0.982TrpVal: 0.982 ± 0.033	0.245TrpTrp: 0.245 ± 0.018	0.338TrpTyr: 0.338 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.709TyrAla: 2.709 ± 0.045	0.27TyrCys: 0.27 ± 0.017	1.476TyrAsp: 1.476 ± 0.037	1.336TyrGlu: 1.336 ± 0.032	0.976TyrPhe: 0.976 ± 0.03	2.367TyrGly: 2.367 ± 0.05	0.486TyrHis: 0.486 ± 0.022	1.121TyrIle: 1.121 ± 0.034	0.842TyrLys: 0.842 ± 0.029	2.548TyrLeu: 2.548 ± 0.047	0.582TyrMet: 0.582 ± 0.025	0.664TyrAsn: 0.664 ± 0.024	1.222TyrPro: 1.222 ± 0.03	1.054TyrGln: 1.054 ± 0.031	1.707TyrArg: 1.707 ± 0.035	1.475TyrSer: 1.475 ± 0.041	1.449TyrThr: 1.449 ± 0.036	1.759TyrVal: 1.759 ± 0.041	0.425TyrTrp: 0.425 ± 0.019	0.672TyrTyr: 0.672 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3751 proteins (1182568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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