Amino acid dipepetide frequency for Lysobacter sp. Root494

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.974AlaAla: 16.974 ± 0.209
1.202AlaCys: 1.202 ± 0.041
6.816AlaAsp: 6.816 ± 0.091
6.845AlaGlu: 6.845 ± 0.105
4.225AlaPhe: 4.225 ± 0.079
10.611AlaGly: 10.611 ± 0.117
2.654AlaHis: 2.654 ± 0.057
5.85AlaIle: 5.85 ± 0.077
4.003AlaLys: 4.003 ± 0.084
14.335AlaLeu: 14.335 ± 0.178
3.418AlaMet: 3.418 ± 0.053
3.231AlaAsn: 3.231 ± 0.067
5.886AlaPro: 5.886 ± 0.098
5.03AlaGln: 5.03 ± 0.088
9.452AlaArg: 9.452 ± 0.108
6.835AlaSer: 6.835 ± 0.092
6.565AlaThr: 6.565 ± 0.097
8.424AlaVal: 8.424 ± 0.094
1.945AlaTrp: 1.945 ± 0.054
2.526AlaTyr: 2.526 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.984CysAla: 0.984 ± 0.033
0.11CysCys: 0.11 ± 0.011
0.499CysAsp: 0.499 ± 0.027
0.492CysGlu: 0.492 ± 0.025
0.29CysPhe: 0.29 ± 0.017
0.889CysGly: 0.889 ± 0.031
0.23CysHis: 0.23 ± 0.015
0.35CysIle: 0.35 ± 0.022
0.21CysLys: 0.21 ± 0.016
0.708CysLeu: 0.708 ± 0.028
0.149CysMet: 0.149 ± 0.014
0.235CysAsn: 0.235 ± 0.017
0.396CysPro: 0.396 ± 0.024
0.207CysGln: 0.207 ± 0.013
0.56CysArg: 0.56 ± 0.022
0.442CysSer: 0.442 ± 0.023
0.461CysThr: 0.461 ± 0.024
0.693CysVal: 0.693 ± 0.029
0.117CysTrp: 0.117 ± 0.013
0.207CysTyr: 0.207 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
8.539AspAla: 8.539 ± 0.116
0.466AspCys: 0.466 ± 0.025
3.36AspAsp: 3.36 ± 0.074
3.709AspGlu: 3.709 ± 0.066
2.194AspPhe: 2.194 ± 0.048
5.398AspGly: 5.398 ± 0.085
1.169AspHis: 1.169 ± 0.037
2.549AspIle: 2.549 ± 0.05
1.602AspLys: 1.602 ± 0.051
5.538AspLeu: 5.538 ± 0.069
1.132AspMet: 1.132 ± 0.036
1.46AspAsn: 1.46 ± 0.043
3.372AspPro: 3.372 ± 0.061
1.501AspGln: 1.501 ± 0.035
4.108AspArg: 4.108 ± 0.073
2.597AspSer: 2.597 ± 0.057
2.858AspThr: 2.858 ± 0.052
4.55AspVal: 4.55 ± 0.074
0.993AspTrp: 0.993 ± 0.034
1.651AspTyr: 1.651 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
7.464GluAla: 7.464 ± 0.116
0.38GluCys: 0.38 ± 0.021
2.621GluAsp: 2.621 ± 0.057
2.755GluGlu: 2.755 ± 0.071
1.957GluPhe: 1.957 ± 0.045
4.252GluGly: 4.252 ± 0.071
1.488GluHis: 1.488 ± 0.043
2.672GluIle: 2.672 ± 0.054
1.719GluLys: 1.719 ± 0.048
6.186GluLeu: 6.186 ± 0.096
1.227GluMet: 1.227 ± 0.041
1.365GluAsn: 1.365 ± 0.039
2.587GluPro: 2.587 ± 0.055
2.495GluGln: 2.495 ± 0.051
5.281GluArg: 5.281 ± 0.084
2.835GluSer: 2.835 ± 0.056
2.904GluThr: 2.904 ± 0.065
4.039GluVal: 4.039 ± 0.074
0.833GluTrp: 0.833 ± 0.028
1.229GluTyr: 1.229 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
4.713PheAla: 4.713 ± 0.08
0.332PheCys: 0.332 ± 0.02
2.844PheAsp: 2.844 ± 0.051
2.145PheGlu: 2.145 ± 0.051
1.205PhePhe: 1.205 ± 0.043
3.484PheGly: 3.484 ± 0.073
0.76PheHis: 0.76 ± 0.026
1.317PheIle: 1.317 ± 0.041
0.896PheLys: 0.896 ± 0.035
3.084PheLeu: 3.084 ± 0.058
0.648PheMet: 0.648 ± 0.028
1.125PheAsn: 1.125 ± 0.035
1.472PhePro: 1.472 ± 0.043
0.92PheGln: 0.92 ± 0.031
2.242PheArg: 2.242 ± 0.054
1.886PheSer: 1.886 ± 0.038
1.868PheThr: 1.868 ± 0.046
2.809PheVal: 2.809 ± 0.058
0.526PheTrp: 0.526 ± 0.026
0.838PheTyr: 0.838 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
8.774GlyAla: 8.774 ± 0.103
0.82GlyCys: 0.82 ± 0.031
4.996GlyAsp: 4.996 ± 0.076
5.222GlyGlu: 5.222 ± 0.088
3.568GlyPhe: 3.568 ± 0.064
6.992GlyGly: 6.992 ± 0.109
2.093GlyHis: 2.093 ± 0.048
4.068GlyIle: 4.068 ± 0.069
3.257GlyLys: 3.257 ± 0.071
8.14GlyLeu: 8.14 ± 0.092
2.172GlyMet: 2.172 ± 0.053
2.636GlyAsn: 2.636 ± 0.06
2.99GlyPro: 2.99 ± 0.054
2.784GlyGln: 2.784 ± 0.061
5.9GlyArg: 5.9 ± 0.086
4.679GlySer: 4.679 ± 0.088
4.712GlyThr: 4.712 ± 0.089
6.574GlyVal: 6.574 ± 0.096
1.603GlyTrp: 1.603 ± 0.047
2.407GlyTyr: 2.407 ± 0.059
0.001GlyXaa: 0.001 ± 0.001
His
3.171HisAla: 3.171 ± 0.058
0.275HisCys: 0.275 ± 0.021
1.341HisAsp: 1.341 ± 0.039
1.29HisGlu: 1.29 ± 0.031
0.88HisPhe: 0.88 ± 0.03
2.317HisGly: 2.317 ± 0.055
0.62HisHis: 0.62 ± 0.03
0.782HisIle: 0.782 ± 0.025
0.495HisLys: 0.495 ± 0.024
2.162HisLeu: 2.162 ± 0.045
0.46HisMet: 0.46 ± 0.025
0.513HisAsn: 0.513 ± 0.02
1.425HisPro: 1.425 ± 0.042
0.592HisGln: 0.592 ± 0.024
1.742HisArg: 1.742 ± 0.044
1.053HisSer: 1.053 ± 0.031
0.951HisThr: 0.951 ± 0.032
1.766HisVal: 1.766 ± 0.045
0.421HisTrp: 0.421 ± 0.023
0.648HisTyr: 0.648 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.912IleAla: 6.912 ± 0.09
0.348IleCys: 0.348 ± 0.021
3.536IleAsp: 3.536 ± 0.063
3.383IleGlu: 3.383 ± 0.064
1.147IlePhe: 1.147 ± 0.042
4.387IleGly: 4.387 ± 0.081
0.834IleHis: 0.834 ± 0.032
1.324IleIle: 1.324 ± 0.041
1.129IleLys: 1.129 ± 0.041
2.932IleLeu: 2.932 ± 0.057
0.51IleMet: 0.51 ± 0.022
1.29IleAsn: 1.29 ± 0.047
2.158IlePro: 2.158 ± 0.046
1.007IleGln: 1.007 ± 0.033
2.923IleArg: 2.923 ± 0.057
2.218IleSer: 2.218 ± 0.058
2.419IleThr: 2.419 ± 0.057
3.533IleVal: 3.533 ± 0.072
0.404IleTrp: 0.404 ± 0.023
0.834IleTyr: 0.834 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.627LysAla: 3.627 ± 0.082
0.147LysCys: 0.147 ± 0.012
1.555LysAsp: 1.555 ± 0.048
1.365LysGlu: 1.365 ± 0.046
0.863LysPhe: 0.863 ± 0.034
2.119LysGly: 2.119 ± 0.055
0.68LysHis: 0.68 ± 0.027
1.272LysIle: 1.272 ± 0.041
1.136LysLys: 1.136 ± 0.054
3.396LysLeu: 3.396 ± 0.058
0.685LysMet: 0.685 ± 0.025
0.783LysAsn: 0.783 ± 0.033
2.225LysPro: 2.225 ± 0.051
1.332LysGln: 1.332 ± 0.038
2.329LysArg: 2.329 ± 0.057
1.591LysSer: 1.591 ± 0.044
1.643LysThr: 1.643 ± 0.044
2.23LysVal: 2.23 ± 0.05
0.359LysTrp: 0.359 ± 0.02
0.7LysTyr: 0.7 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
14.034LeuAla: 14.034 ± 0.13
0.871LeuCys: 0.871 ± 0.031
6.524LeuAsp: 6.524 ± 0.083
5.376LeuGlu: 5.376 ± 0.091
3.421LeuPhe: 3.421 ± 0.07
8.305LeuGly: 8.305 ± 0.111
2.54LeuHis: 2.54 ± 0.054
3.917LeuIle: 3.917 ± 0.075
3.135LeuLys: 3.135 ± 0.066
11.163LeuLeu: 11.163 ± 0.145
2.129LeuMet: 2.129 ± 0.047
2.366LeuAsn: 2.366 ± 0.043
6.217LeuPro: 6.217 ± 0.088
3.848LeuGln: 3.848 ± 0.072
8.848LeuArg: 8.848 ± 0.107
5.725LeuSer: 5.725 ± 0.074
4.771LeuThr: 4.771 ± 0.066
7.709LeuVal: 7.709 ± 0.085
1.344LeuTrp: 1.344 ± 0.047
2.113LeuTyr: 2.113 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.624MetAla: 2.624 ± 0.054
0.142MetCys: 0.142 ± 0.013
1.122MetAsp: 1.122 ± 0.037
0.994MetGlu: 0.994 ± 0.032
0.675MetPhe: 0.675 ± 0.028
1.503MetGly: 1.503 ± 0.039
0.531MetHis: 0.531 ± 0.024
0.833MetIle: 0.833 ± 0.036
0.957MetLys: 0.957 ± 0.029
2.44MetLeu: 2.44 ± 0.058
0.454MetMet: 0.454 ± 0.024
0.754MetAsn: 0.754 ± 0.033
1.51MetPro: 1.51 ± 0.043
0.947MetGln: 0.947 ± 0.031
1.809MetArg: 1.809 ± 0.043
1.423MetSer: 1.423 ± 0.039
1.402MetThr: 1.402 ± 0.044
1.483MetVal: 1.483 ± 0.04
0.206MetTrp: 0.206 ± 0.014
0.395MetTyr: 0.395 ± 0.022
0.001MetXaa: 0.001 ± 0.001
Asn
3.694AsnAla: 3.694 ± 0.071
0.242AsnCys: 0.242 ± 0.018
1.591AsnAsp: 1.591 ± 0.037
1.365AsnGlu: 1.365 ± 0.042
0.961AsnPhe: 0.961 ± 0.03
2.613AsnGly: 2.613 ± 0.059
0.538AsnHis: 0.538 ± 0.022
1.168AsnIle: 1.168 ± 0.039
0.738AsnLys: 0.738 ± 0.031
2.54AsnLeu: 2.54 ± 0.052
0.467AsnMet: 0.467 ± 0.023
0.733AsnAsn: 0.733 ± 0.029
1.828AsnPro: 1.828 ± 0.043
0.788AsnGln: 0.788 ± 0.033
1.839AsnArg: 1.839 ± 0.045
1.266AsnSer: 1.266 ± 0.043
1.395AsnThr: 1.395 ± 0.037
2.01AsnVal: 2.01 ± 0.048
0.387AsnTrp: 0.387 ± 0.025
0.689AsnTyr: 0.689 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.73ProAla: 6.73 ± 0.107
0.308ProCys: 0.308 ± 0.02
3.529ProAsp: 3.529 ± 0.062
3.402ProGlu: 3.402 ± 0.061
1.698ProPhe: 1.698 ± 0.042
4.708ProGly: 4.708 ± 0.074
1.144ProHis: 1.144 ± 0.035
1.932ProIle: 1.932 ± 0.05
1.474ProLys: 1.474 ± 0.039
5.218ProLeu: 5.218 ± 0.088
1.268ProMet: 1.268 ± 0.035
1.313ProAsn: 1.313 ± 0.039
2.617ProPro: 2.617 ± 0.061
2.032ProGln: 2.032 ± 0.048
3.47ProArg: 3.47 ± 0.075
2.759ProSer: 2.759 ± 0.055
2.339ProThr: 2.339 ± 0.051
4.158ProVal: 4.158 ± 0.067
0.795ProTrp: 0.795 ± 0.03
1.199ProTyr: 1.199 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
4.633GlnAla: 4.633 ± 0.086
0.264GlnCys: 0.264 ± 0.017
1.463GlnAsp: 1.463 ± 0.035
1.43GlnGlu: 1.43 ± 0.04
1.209GlnPhe: 1.209 ± 0.035
2.721GlnGly: 2.721 ± 0.062
0.91GlnHis: 0.91 ± 0.033
1.496GlnIle: 1.496 ± 0.039
0.977GlnLys: 0.977 ± 0.036
4.188GlnLeu: 4.188 ± 0.061
0.805GlnMet: 0.805 ± 0.03
0.756GlnAsn: 0.756 ± 0.029
2.173GlnPro: 2.173 ± 0.043
1.791GlnGln: 1.791 ± 0.048
3.486GlnArg: 3.486 ± 0.069
1.758GlnSer: 1.758 ± 0.04
1.473GlnThr: 1.473 ± 0.038
2.773GlnVal: 2.773 ± 0.052
0.563GlnTrp: 0.563 ± 0.023
0.747GlnTyr: 0.747 ± 0.027
0.001GlnXaa: 0.001 ± 0.001
Arg
8.486ArgAla: 8.486 ± 0.117
0.537ArgCys: 0.537 ± 0.025
4.81ArgAsp: 4.81 ± 0.073
5.216ArgGlu: 5.216 ± 0.083
3.061ArgPhe: 3.061 ± 0.064
5.527ArgGly: 5.527 ± 0.089
1.875ArgHis: 1.875 ± 0.046
4.086ArgIle: 4.086 ± 0.075
2.296ArgLys: 2.296 ± 0.059
8.014ArgLeu: 8.014 ± 0.111
2.02ArgMet: 2.02 ± 0.048
2.298ArgAsn: 2.298 ± 0.051
3.258ArgPro: 3.258 ± 0.06
2.707ArgGln: 2.707 ± 0.057
5.892ArgArg: 5.892 ± 0.098
3.677ArgSer: 3.677 ± 0.066
3.499ArgThr: 3.499 ± 0.061
5.431ArgVal: 5.431 ± 0.084
1.434ArgTrp: 1.434 ± 0.044
2.245ArgTyr: 2.245 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
6.074SerAla: 6.074 ± 0.078
0.389SerCys: 0.389 ± 0.024
2.943SerAsp: 2.943 ± 0.061
2.712SerGlu: 2.712 ± 0.058
1.994SerPhe: 1.994 ± 0.052
5.194SerGly: 5.194 ± 0.087
1.118SerHis: 1.118 ± 0.031
2.259SerIle: 2.259 ± 0.054
1.588SerLys: 1.588 ± 0.046
5.412SerLeu: 5.412 ± 0.078
1.136SerMet: 1.136 ± 0.032
1.556SerAsn: 1.556 ± 0.048
2.784SerPro: 2.784 ± 0.053
1.942SerGln: 1.942 ± 0.047
3.925SerArg: 3.925 ± 0.064
2.896SerSer: 2.896 ± 0.065
2.869SerThr: 2.869 ± 0.064
3.733SerVal: 3.733 ± 0.068
0.783SerTrp: 0.783 ± 0.033
1.325SerTyr: 1.325 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
5.941ThrAla: 5.941 ± 0.083
0.427ThrCys: 0.427 ± 0.023
2.661ThrAsp: 2.661 ± 0.055
2.255ThrGlu: 2.255 ± 0.05
1.635ThrPhe: 1.635 ± 0.047
4.882ThrGly: 4.882 ± 0.089
1.191ThrHis: 1.191 ± 0.035
2.262ThrIle: 2.262 ± 0.053
1.22ThrLys: 1.22 ± 0.042
5.916ThrLeu: 5.916 ± 0.084
1.049ThrMet: 1.049 ± 0.029
1.177ThrAsn: 1.177 ± 0.04
3.4ThrPro: 3.4 ± 0.058
1.735ThrGln: 1.735 ± 0.042
3.747ThrArg: 3.747 ± 0.06
2.678ThrSer: 2.678 ± 0.06
2.864ThrThr: 2.864 ± 0.073
4.069ThrVal: 4.069 ± 0.075
0.702ThrTrp: 0.702 ± 0.027
1.138ThrTyr: 1.138 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
9.32ValAla: 9.32 ± 0.093
0.629ValCys: 0.629 ± 0.026
4.392ValAsp: 4.392 ± 0.072
4.478ValGlu: 4.478 ± 0.073
2.661ValPhe: 2.661 ± 0.06
5.361ValGly: 5.361 ± 0.082
1.658ValHis: 1.658 ± 0.047
3.598ValIle: 3.598 ± 0.077
2.076ValLys: 2.076 ± 0.045
8.407ValLeu: 8.407 ± 0.104
1.611ValMet: 1.611 ± 0.043
2.067ValAsn: 2.067 ± 0.053
3.889ValPro: 3.889 ± 0.063
2.477ValGln: 2.477 ± 0.059
5.351ValArg: 5.351 ± 0.077
4.14ValSer: 4.14 ± 0.067
3.981ValThr: 3.981 ± 0.072
6.031ValVal: 6.031 ± 0.098
0.886ValTrp: 0.886 ± 0.031
1.604ValTyr: 1.604 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.211TrpAla: 1.211 ± 0.043
0.147TrpCys: 0.147 ± 0.012
0.668TrpAsp: 0.668 ± 0.025
0.602TrpGlu: 0.602 ± 0.028
0.57TrpPhe: 0.57 ± 0.026
0.941TrpGly: 0.941 ± 0.033
0.379TrpHis: 0.379 ± 0.02
0.719TrpIle: 0.719 ± 0.031
0.554TrpLys: 0.554 ± 0.025
2.19TrpLeu: 2.19 ± 0.053
0.435TrpMet: 0.435 ± 0.021
0.551TrpAsn: 0.551 ± 0.027
0.761TrpPro: 0.761 ± 0.032
0.701TrpGln: 0.701 ± 0.029
1.418TrpArg: 1.418 ± 0.04
0.908TrpSer: 0.908 ± 0.038
0.736TrpThr: 0.736 ± 0.026
0.868TrpVal: 0.868 ± 0.03
0.306TrpTrp: 0.306 ± 0.021
0.368TrpTyr: 0.368 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.92TyrAla: 2.92 ± 0.053
0.195TyrCys: 0.195 ± 0.014
1.475TyrAsp: 1.475 ± 0.045
1.253TyrGlu: 1.253 ± 0.04
0.901TyrPhe: 0.901 ± 0.035
2.188TyrGly: 2.188 ± 0.048
0.489TyrHis: 0.489 ± 0.021
0.773TyrIle: 0.773 ± 0.027
0.572TyrLys: 0.572 ± 0.025
2.386TyrLeu: 2.386 ± 0.047
0.429TyrMet: 0.429 ± 0.021
0.642TyrAsn: 0.642 ± 0.033
1.143TyrPro: 1.143 ± 0.035
0.78TyrGln: 0.78 ± 0.027
2.05TyrArg: 2.05 ± 0.049
1.267TyrSer: 1.267 ± 0.036
1.241TyrThr: 1.241 ± 0.043
1.734TyrVal: 1.734 ± 0.043
0.428TyrTrp: 0.428 ± 0.023
0.654TyrTyr: 0.654 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.001XaaGln: 0.001 ± 0.001
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.001XaaVal: 0.001 ± 0.001
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3007 proteins (981173 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski