Calocera viscosa (strain TUFC12733)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Dacrymycetes; Dacrymycetales; Dacrymycetaceae; Calocera; Calocera viscosa

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12323 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167HV73|A0A167HV73_CALVF Uncharacterized protein (Fragment) OS=Calocera viscosa (strain TUFC12733) OX=1330018 GN=CALVIDRAFT_463058 PE=4 SV=1
MM1 pKa = 7.01QRR3 pKa = 11.84LSALVLALLSASQLAHH19 pKa = 6.96ALPLRR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 4.28VPQEE30 pKa = 3.98HH31 pKa = 6.06SHH33 pKa = 5.98QPFLTSVQTSLQLNNPDD50 pKa = 4.75GITDD54 pKa = 3.75SVFGLLGDD62 pKa = 4.22AAAAAGAGKK71 pKa = 8.25ITDD74 pKa = 3.94MDD76 pKa = 4.44CLQQATADD84 pKa = 3.63QAFTNAKK91 pKa = 9.95AAGDD95 pKa = 3.64VQGQVDD101 pKa = 3.91ALIYY105 pKa = 10.19RR106 pKa = 11.84ALEE109 pKa = 4.22RR110 pKa = 11.84NTGAIGLASAACTSLTAVNPEE131 pKa = 3.4IAAIAQHH138 pKa = 5.94QDD140 pKa = 3.26PASSNAAAVNKK151 pKa = 10.55AIVLTLAQQIAAVGGNPLDD170 pKa = 4.76AIKK173 pKa = 10.75SGTFAPGTIGDD184 pKa = 3.82PTAAGNTCDD193 pKa = 5.38DD194 pKa = 4.24ANDD197 pKa = 3.79ANGCIFTQNLLVPDD211 pKa = 4.64ASDD214 pKa = 4.37DD215 pKa = 4.35EE216 pKa = 4.96IDD218 pKa = 3.64AAVAGISSSSGNSTTTDD235 pKa = 2.9SSTGSTDD242 pKa = 3.08TSSTDD247 pKa = 3.19SDD249 pKa = 4.38SDD251 pKa = 3.86TATCPPPSTVTVTVTSTAAAEE272 pKa = 4.19TATGSSSAAAATSTAISTASAGTGSSTASSNGTNLQTFTGALGGILPPAVVAGGEE327 pKa = 4.46GFITDD332 pKa = 3.67NGSQFVNLAAALGRR346 pKa = 11.84SCDD349 pKa = 3.71VQHH352 pKa = 6.16NQCADD357 pKa = 3.2QANSQNQSPFSVGDD371 pKa = 3.98CDD373 pKa = 4.8TQDD376 pKa = 4.05TEE378 pKa = 4.69CKK380 pKa = 10.19AAGTGGSTTGTSAASSSTASTTTTQAAAVGATSGSISTVATSGTNLQTFTGALGGILPPPVVAGGEE446 pKa = 4.27GFITDD451 pKa = 3.67NGSQFVNLAAALGRR465 pKa = 11.84SCDD468 pKa = 3.67MQHH471 pKa = 5.92NQCADD476 pKa = 3.61LANSQHH482 pKa = 5.54QFSVSDD488 pKa = 4.24CDD490 pKa = 4.52QQDD493 pKa = 4.2TEE495 pKa = 4.78CKK497 pKa = 10.09AAA499 pKa = 4.27

Molecular weight: 49.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167LED9|A0A167LED9_CALVF Uncharacterized protein OS=Calocera viscosa (strain TUFC12733) OX=1330018 GN=CALVIDRAFT_537994 PE=4 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84HH4 pKa = 6.71LIRR7 pKa = 11.84RR8 pKa = 11.84AITAAARR15 pKa = 11.84TATRR19 pKa = 11.84PAAPTTTAVSAVALVRR35 pKa = 11.84PSSSRR40 pKa = 11.84IGTSTLPSPFRR51 pKa = 11.84TLTPFSPRR59 pKa = 11.84PSALTTLSPVRR70 pKa = 11.84PSIALSPLTAALPLLQARR88 pKa = 11.84FLGNEE93 pKa = 3.95YY94 pKa = 10.37QPSQRR99 pKa = 11.84KK100 pKa = 8.82RR101 pKa = 11.84KK102 pKa = 9.39RR103 pKa = 11.84KK104 pKa = 9.34FGFLQRR110 pKa = 11.84LRR112 pKa = 11.84SKK114 pKa = 10.83NGRR117 pKa = 11.84KK118 pKa = 7.28TLAARR123 pKa = 11.84RR124 pKa = 11.84WKK126 pKa = 9.4GRR128 pKa = 11.84RR129 pKa = 11.84YY130 pKa = 9.93LSHH133 pKa = 7.01

Molecular weight: 14.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12317

6

12323

5084671

49

5013

412.6

45.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.386 ± 0.023

1.257 ± 0.009

5.323 ± 0.017

6.157 ± 0.025

3.483 ± 0.016

7.059 ± 0.02

2.426 ± 0.01

4.419 ± 0.015

4.133 ± 0.021

9.6 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.009

2.999 ± 0.012

6.895 ± 0.03

3.798 ± 0.017

6.503 ± 0.021

7.886 ± 0.025

5.834 ± 0.013

6.391 ± 0.016

1.581 ± 0.009

2.634 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski